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The map label for this gene is proS

Identifier: 15603235

GI number: 15603235

Start: 1561161

End: 1562876

Strand: Reverse

Name: proS

Synonym: PM1370

Alternate gene names: 15603235

Gene position: 1562876-1561161 (Counterclockwise)

Preceding gene: 15603236

Following gene: 15603233

Centisome position: 69.23

GC content: 43.76

Gene sequence:

>1716_bases
ATGCGTACAAGTCAATATTTATTCTCAACCTTAAAAGAAACGCCTGCTGAGGCGAGTATTGTTAGCCATCAATTAATGTT
ACGTGCGGGTATGATTCGTCCGTTAGCATCGGGGCTCTATACTTGGTTGCCGACCGGGTTGCGTGTCTTAAATAAAGTAG
AAAAGATTATCCGTGAAGAAATGGATAAAAGCGGTGCGTTAGAAGTGAAAATGGCGGTCACGCAACCAGCAGAATTATGG
CAAGAGTCTGGTCGTTGGGAAGAATATGGTCCTGAGTTATTGCGCTTTAAAGATCGTGGCGAGCGTGATTTTGTGATTGG
ACCAACCAATGAAGAAGTGATTACCGATTTAGTGCGCCGTGAATTGAGTTCTTATAAGCAGTTACCATTGAATTTATATC
ATATTCAAACCAAATTCCGTGATGAAGTTCGTCCGCGTTTCGGCGTAATGCGTTCGCGTGAGTTTGTGATGAAAGATGCA
TACTCTTTCCATACTACTCATGAGTGTTTGCAAAAAACCTATGATGTGATGTATGAAACCTATTCGAATATTTTTAACCG
CTTAGGGTTAGATTTTCGCGCAGTGCAAGCGGATACAGGTTCAATTGGTGGGAGTGCATCTCATGAATTCCAAGTGCTAG
CACAAAGTGGTGAAGATGATGTGGTGTTTTCCACCGAGTCAGATTTTGCCGCAAACATTGAACTTGCCGAAGCGGTGGCA
TTAGGTGAACGTGGTGCGGCAACAGAAGAATTACGTGTAGTCGATACGCCTAATGCAAAAACCATTGCGGAATTGGTAGA
ACAATTCAATCAACCGATTGAAAAAACCGTCAAAACCTTAGTCGTCCACGCGACGGAAGAAAGTGGTCATAAATTGGTAG
CATTGTTAGTGCGTGGTGATCACGAGTTAAACGAAATTAAAGCGGAAAAAGTCGATATCGTGGCAAGCCCGTTACAATTC
GCCACAGACGAAGAAATCCGTGCGGTGGTGGGCGCTGGTACGGGTTCACTGGGTCCGATTAATTTACCAATGCCGATAGT
GATTGATCGCACCGTCGCCAATATGGATAACTTCTCAGCCGGTGCAAATCAAGATGGTAAGCATTATTTTGGTATTAACT
GGGAACGTGATTTACCTGTGCCACACATTGCCGATTTGCGTAATGTAGTGGAAGGCGATCCAAGTCCAGATGGTAAAGGT
GTGCTACAAATTAAACGTGGTATCGAGGTTGGGCATATTTTCCAATTAGGCACCAAATATTCTGCGGCAATGAACGCCAC
TGTACAAGGTGAAGATGGTCGCCCGCAAACTATGATTATGGGCTGTTATGGGATTGGTGTTACTCGTGTGATTGCGGCTG
CGATTGAACAGCATCATGATGAGCGTGGCATTATTTGGCCAGACAATATTGCCCCATTCAAAGTGGCGATTGTGCCGATG
AATATGCATAAATCCGAAAGCGTGCAACAATTTGCGGAAGAATTATACCGCACTTTAACTGCACAGGGCGTGGAAGTGAT
TTTTGATGATCGTAAAGAGCGTCCCGGTGTGATGTTTGCGGATATGGAATTGATCGGCGTGCCACACATGATTGTGATTG
GTGAGAAAAATCTCGAGAAAGGTGAAATTGAATATAAATATCGTCGCTCTGGTGAAAAAGAAATGATTGCCAAAGATCAA
TTATTAGATGTCTTAAAGGCGAAATTCGCCAGCTAA

Upstream 100 bases:

>100_bases
TGCAAATTTGCGTATAACTCTTTGTGCAAACCCCACACAGTGATAAACTAACCCACAATTTTTTCGATTTATAAACCGTT
TTTCATTTACAGGTATCTCA

Downstream 100 bases:

>100_bases
GTCGATGAATAGACAACAAAAAATGCGAGTTAATTACTCGCATTTTTTATGTTTGAAAAGCGAAAATAACGACGTAATTA
TTTTCTGGCTAATGGTGGCA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTSQYLFSTLKETPAEASIVSHQLMLRAGMIRPLASGLYTWLPTGLRVLNKVEKIIREEMDKSGALEVKMAVTQPAELW
QESGRWEEYGPELLRFKDRGERDFVIGPTNEEVITDLVRRELSSYKQLPLNLYHIQTKFRDEVRPRFGVMRSREFVMKDA
YSFHTTHECLQKTYDVMYETYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSTESDFAANIELAEAVA
LGERGAATEELRVVDTPNAKTIAELVEQFNQPIEKTVKTLVVHATEESGHKLVALLVRGDHELNEIKAEKVDIVASPLQF
ATDEEIRAVVGAGTGSLGPINLPMPIVIDRTVANMDNFSAGANQDGKHYFGINWERDLPVPHIADLRNVVEGDPSPDGKG
VLQIKRGIEVGHIFQLGTKYSAAMNATVQGEDGRPQTMIMGCYGIGVTRVIAAAIEQHHDERGIIWPDNIAPFKVAIVPM
NMHKSESVQQFAEELYRTLTAQGVEVIFDDRKERPGVMFADMELIGVPHMIVIGEKNLEKGEIEYKYRRSGEKEMIAKDQ
LLDVLKAKFAS

Sequences:

>Translated_571_residues
MRTSQYLFSTLKETPAEASIVSHQLMLRAGMIRPLASGLYTWLPTGLRVLNKVEKIIREEMDKSGALEVKMAVTQPAELW
QESGRWEEYGPELLRFKDRGERDFVIGPTNEEVITDLVRRELSSYKQLPLNLYHIQTKFRDEVRPRFGVMRSREFVMKDA
YSFHTTHECLQKTYDVMYETYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSTESDFAANIELAEAVA
LGERGAATEELRVVDTPNAKTIAELVEQFNQPIEKTVKTLVVHATEESGHKLVALLVRGDHELNEIKAEKVDIVASPLQF
ATDEEIRAVVGAGTGSLGPINLPMPIVIDRTVANMDNFSAGANQDGKHYFGINWERDLPVPHIADLRNVVEGDPSPDGKG
VLQIKRGIEVGHIFQLGTKYSAAMNATVQGEDGRPQTMIMGCYGIGVTRVIAAAIEQHHDERGIIWPDNIAPFKVAIVPM
NMHKSESVQQFAEELYRTLTAQGVEVIFDDRKERPGVMFADMELIGVPHMIVIGEKNLEKGEIEYKYRRSGEKEMIAKDQ
LLDVLKAKFAS
>Mature_571_residues
MRTSQYLFSTLKETPAEASIVSHQLMLRAGMIRPLASGLYTWLPTGLRVLNKVEKIIREEMDKSGALEVKMAVTQPAELW
QESGRWEEYGPELLRFKDRGERDFVIGPTNEEVITDLVRRELSSYKQLPLNLYHIQTKFRDEVRPRFGVMRSREFVMKDA
YSFHTTHECLQKTYDVMYETYSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSTESDFAANIELAEAVA
LGERGAATEELRVVDTPNAKTIAELVEQFNQPIEKTVKTLVVHATEESGHKLVALLVRGDHELNEIKAEKVDIVASPLQF
ATDEEIRAVVGAGTGSLGPINLPMPIVIDRTVANMDNFSAGANQDGKHYFGINWERDLPVPHIADLRNVVEGDPSPDGKG
VLQIKRGIEVGHIFQLGTKYSAAMNATVQGEDGRPQTMIMGCYGIGVTRVIAAAIEQHHDERGIIWPDNIAPFKVAIVPM
NMHKSESVQQFAEELYRTLTAQGVEVIFDDRKERPGVMFADMELIGVPHMIVIGEKNLEKGEIEYKYRRSGEKEMIAKDQ
LLDVLKAKFAS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=44.6902654867257, Blast_Score=218, Evalue=2e-56,
Organism=Escherichia coli, GI1786392, Length=566, Percent_Identity=76.5017667844523, Blast_Score=915, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=209, Percent_Identity=42.5837320574163, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=92, Percent_Identity=36.9565217391304, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=554, Percent_Identity=30.8664259927798, Blast_Score=249, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321808, Length=181, Percent_Identity=25.9668508287293, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24656200, Length=220, Percent_Identity=34.5454545454545, Blast_Score=162, Evalue=7e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PASMU (Q9CL72)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246309.1
- ProteinModelPortal:   Q9CL72
- SMR:   Q9CL72
- GeneID:   1244717
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1370
- NMPDR:   fig|272843.1.peg.1371
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   PMUL272843:PM1370-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63894; Mature: 63894

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLFSTLKETPAEASIVSHQLMLRAGMIRPLASGLYTWLPTGLRVLNKVEKIIREE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDKSGALEVKMAVTQPAELWQESGRWEEYGPELLRFKDRGERDFVIGPTNEEVITDLVRR
HCCCCCEEEEEEECCCHHHHHHCCCCHHHCCHHHHHHCCCCCEEEECCCCHHHHHHHHHH
ELSSYKQLPLNLYHIQTKFRDEVRPRFGVMRSREFVMKDAYSFHTTHECLQKTYDVMYET
HHHHHHCCCCEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSTESDFAANIELAEAVA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHH
LGERGAATEELRVVDTPNAKTIAELVEQFNQPIEKTVKTLVVHATEESGHKLVALLVRGD
HCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHHHHHEEEECCCCCCEEEEEEEECC
HELNEIKAEKVDIVASPLQFATDEEIRAVVGAGTGSLGPINLPMPIVIDRTVANMDNFSA
CHHHHHHHHHHHEEECCHHHCCCHHHHHEEECCCCCCCCCCCCCCEEEEHHHHCCCCCCC
GANQDGKHYFGINWERDLPVPHIADLRNVVEGDPSPDGKGVLQIKRGIEVGHIFQLGTKY
CCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEEHHHHCCCCH
SAAMNATVQGEDGRPQTMIMGCYGIGVTRVIAAAIEQHHDERGIIWPDNIAPFKVAIVPM
HHHEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
NMHKSESVQQFAEELYRTLTAQGVEVIFDDRKERPGVMFADMELIGVPHMIVIGEKNLEK
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHCCCEEEEEECCCCCC
GEIEYKYRRSGEKEMIAKDQLLDVLKAKFAS
CCEEEHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSQYLFSTLKETPAEASIVSHQLMLRAGMIRPLASGLYTWLPTGLRVLNKVEKIIREE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDKSGALEVKMAVTQPAELWQESGRWEEYGPELLRFKDRGERDFVIGPTNEEVITDLVRR
HCCCCCEEEEEEECCCHHHHHHCCCCHHHCCHHHHHHCCCCCEEEECCCCHHHHHHHHHH
ELSSYKQLPLNLYHIQTKFRDEVRPRFGVMRSREFVMKDAYSFHTTHECLQKTYDVMYET
HHHHHHCCCCEEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSNIFNRLGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSTESDFAANIELAEAVA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHH
LGERGAATEELRVVDTPNAKTIAELVEQFNQPIEKTVKTLVVHATEESGHKLVALLVRGD
HCCCCCCCCCEEEEECCCHHHHHHHHHHHCHHHHHHHHHHEEEECCCCCCEEEEEEEECC
HELNEIKAEKVDIVASPLQFATDEEIRAVVGAGTGSLGPINLPMPIVIDRTVANMDNFSA
CHHHHHHHHHHHEEECCHHHCCCHHHHHEEECCCCCCCCCCCCCCEEEEHHHHCCCCCCC
GANQDGKHYFGINWERDLPVPHIADLRNVVEGDPSPDGKGVLQIKRGIEVGHIFQLGTKY
CCCCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCEEHHHHCCCCH
SAAMNATVQGEDGRPQTMIMGCYGIGVTRVIAAAIEQHHDERGIIWPDNIAPFKVAIVPM
HHHEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEE
NMHKSESVQQFAEELYRTLTAQGVEVIFDDRKERPGVMFADMELIGVPHMIVIGEKNLEK
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECCHHHCCCEEEEEECCCCCC
GEIEYKYRRSGEKEMIAKDQLLDVLKAKFAS
CCEEEHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100