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The map label for this gene is rfaD

Identifier: 15603207

GI number: 15603207

Start: 1535072

End: 1536004

Strand: Reverse

Name: rfaD

Synonym: PM1342

Alternate gene names: 15603207

Gene position: 1536004-1535072 (Counterclockwise)

Preceding gene: 15603210

Following gene: 15603206

Centisome position: 68.04

GC content: 39.44

Gene sequence:

>933_bases
ATGATTATCGTAACTGGCGGTGCTGGCTTTATTGGAAGTAACATTGTAAAAGCCTTAAACGAATTAGGGCGTACGGATAT
TTTAGTGGTAGATAATTTAAAAGATGGTACAAAGTTCGCTAATTTAGTGGATTTGGATATTGCAGATTATTGTGACAAAG
AAGATTTTATTGCCTCGATCATCGCTGGTGATGATTTAGGGGATATTGAGGTCATTTTCCACGAAGGCGCCTGTTCTGCT
ACTACCGAATGGGACGGCAAGTACATCATGCACAATAACTATGAATATTCCAAAGAGTTATTGCATTATTGTTTAGATCG
TGAAATTCCGTTCTTTTATGCTTCATCTGCAGCAACTTATGGTGATAAAACTGAATTCCGTGAAGAACGCGAATTTGAAG
CACCATTAAATGTTTACGGCTATTCCAAATTCTTATTTGATCAATATGTACGTGCTATTTTACCAGAAGCTAACTCACCT
GTTTGTGGCTTCCGTTATTTCAACGTGTACGGTCCACGTGAGAATCACAAAGGCGCGATGGCAAGCGTGGCATTCCACTT
AAATAACCAAATCTTAAAAGGGGAAAAACCAAAATTATTTGCAGGCAGTGAACATTTCCGGCGTGACTTTGTGTATGTGG
GCGATGTGGCTGCTGTGAATATTTGGTGTTGGCAAAACAACATTTCTGGCATCTATAACTTAGGAACAGGAAATGCGGAA
TCTTTCCTTGAAGTAGCAAAAGCCGTCGTGAAGTTCCATGGTAAAACGGAAGCAGATATTGAAATCATTCCATTCCCAGA
ACATTTAAAAAGCCGTTACCAAGAATACACACAAGCCAATTTAGATAAATTACGCGCCACTGGCTATGACAAACCGTTTA
AAACGGTCGCACAGGGTGTAGCAGAATATATGGCATGGTTAAACCGTAAATAA

Upstream 100 bases:

>100_bases
TCTCCTTGAGAATTTTCCAGCTAAAGAGAAAATACTTAAACACAACCCACTGAAAATCCATTACAATACCCGTAATATTA
ACCAACAGAGGACAAGCTTT

Downstream 100 bases:

>100_bases
TAAATAAGCGGCTTCATCACATAGCGTCCAACGTTGGGCGCTATTTTTTTGCCTAACTAAACGTATAAAACAGCAACCGC
ACTTCGAGTATGCCAACTCG

Product: ADP-L-glycero-D-mannoheptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MIIVTGGAGFIGSNIVKALNELGRTDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDDLGDIEVIFHEGACSA
TTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDKTEFREEREFEAPLNVYGYSKFLFDQYVRAILPEANSP
VCGFRYFNVYGPRENHKGAMASVAFHLNNQILKGEKPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNNISGIYNLGTGNAE
SFLEVAKAVVKFHGKTEADIEIIPFPEHLKSRYQEYTQANLDKLRATGYDKPFKTVAQGVAEYMAWLNRK

Sequences:

>Translated_310_residues
MIIVTGGAGFIGSNIVKALNELGRTDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDDLGDIEVIFHEGACSA
TTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDKTEFREEREFEAPLNVYGYSKFLFDQYVRAILPEANSP
VCGFRYFNVYGPRENHKGAMASVAFHLNNQILKGEKPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNNISGIYNLGTGNAE
SFLEVAKAVVKFHGKTEADIEIIPFPEHLKSRYQEYTQANLDKLRATGYDKPFKTVAQGVAEYMAWLNRK
>Mature_310_residues
MIIVTGGAGFIGSNIVKALNELGRTDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASIIAGDDLGDIEVIFHEGACSA
TTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATYGDKTEFREEREFEAPLNVYGYSKFLFDQYVRAILPEANSP
VCGFRYFNVYGPRENHKGAMASVAFHLNNQILKGEKPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNNISGIYNLGTGNAE
SFLEVAKAVVKFHGKTEADIEIIPFPEHLKSRYQEYTQANLDKLRATGYDKPFKTVAQGVAEYMAWLNRK

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=310, Percent_Identity=75.1612903225806, Blast_Score=487, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71982035, Length=324, Percent_Identity=28.0864197530864, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=326, Percent_Identity=27.9141104294479, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_PASMU (Q9CL97)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246281.1
- ProteinModelPortal:   Q9CL97
- SMR:   Q9CL97
- GeneID:   1244689
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1342
- NMPDR:   fig|272843.1.peg.1343
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- ProtClustDB:   PRK11150
- BioCyc:   PMUL272843:PM1342-MONOMER
- BRENDA:   5.1.3.20
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 35055; Mature: 35055

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: ACT_SITE 139-139 ACT_SITE 177-177 BINDING 38-38 BINDING 53-53 BINDING 92-92 BINDING 143-143 BINDING 168-168 BINDING 169-169 BINDING 177-177 BINDING 179-179 BINDING 186-186 BINDING 208-208 BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTGGAGFIGSNIVKALNELGRTDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASI
CEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHEEECCHHHHCCHHHHHHHH
IAGDDLGDIEVIFHEGACSATTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATY
HCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCC
GDKTEFREEREFEAPLNVYGYSKFLFDQYVRAILPEANSPVCGFRYFNVYGPRENHKGAM
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHH
ASVAFHLNNQILKGEKPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNNISGIYNLGTGNAE
HHEEEHHCCCEECCCCCCEEECCHHHHHCEEEECCEEEEEEEEEECCCCEEEECCCCCHH
SFLEVAKAVVKFHGKTEADIEIIPFPEHLKSRYQEYTQANLDKLRATGYDKPFKTVAQGV
HHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AEYMAWLNRK
HHHHHHHCCC
>Mature Secondary Structure
MIIVTGGAGFIGSNIVKALNELGRTDILVVDNLKDGTKFANLVDLDIADYCDKEDFIASI
CEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHEEECCHHHHCCHHHHHHHH
IAGDDLGDIEVIFHEGACSATTEWDGKYIMHNNYEYSKELLHYCLDREIPFFYASSAATY
HCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCC
GDKTEFREEREFEAPLNVYGYSKFLFDQYVRAILPEANSPVCGFRYFNVYGPRENHKGAM
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHH
ASVAFHLNNQILKGEKPKLFAGSEHFRRDFVYVGDVAAVNIWCWQNNISGIYNLGTGNAE
HHEEEHHCCCEECCCCCCEEECCHHHHHCEEEECCEEEEEEEEEECCCCEEEECCCCCHH
SFLEVAKAVVKFHGKTEADIEIIPFPEHLKSRYQEYTQANLDKLRATGYDKPFKTVAQGV
HHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
AEYMAWLNRK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100