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The map label for this gene is hisA

Identifier: 15603068

GI number: 15603068

Start: 1396909

End: 1397658

Strand: Direct

Name: hisA

Synonym: PM1203

Alternate gene names: 15603068

Gene position: 1396909-1397658 (Clockwise)

Preceding gene: 15603067

Following gene: 15603069

Centisome position: 61.88

GC content: 45.33

Gene sequence:

>750_bases
ATGAAAACATCACAAATTATCCCCGCTCTTGATCTGATTGATGGTCAAGTGGTGCGCCTTTATCAAGGTGATTATGGACA
GAAAACCCTTTATTCCGATAACCCGATAGCTCAATTCCAAGATTATGTGGCACAAGGGGCAAAGTACTTACATTTAGTGG
ATCTGACTGGCGCGAAAGATCCAACTAAACGCCAAACGCAACTCATTGGTGAAATCATCAACGCAGTAAATTGCTCAATT
CAAGTGGGTGGCGGTATTCGTACTGAGCAAGATGTGGCAGATTTACTGGCGGTGGGCGCAAATCGTGTAGTGATTGGCTC
AACAGCAGTCAAGCAACCCGAAATGGTCAAACAATGGTTTAAAAAATACGGGGCAGAAAAATTTGTCTTAGCGTTAGATG
TCAACATTAACGCACAAGGCGAAAAACAGATCGCAGTGAGCGGTTGGCAAGAAAACAGCGGCGTGTCTTTAGAAGCCTTA
ATTGCCGATTTCCGTGAAGTCGGTTTGCAACACGTGTTATGTACCGATATTTCTAAAGATGGTACCCTGCAAGGTTCTAA
TGTAGCGCTATATCAAGAAATCTGTACCCAATTCCCGACGATTCAATTCCAATCATCAGGCGGGATCGGTTCCATCGCGG
ATATTGACGCCTTAAAAGGCACGGGGGTCGCTGGTGTGATTGTGGGGCGTGCTTTATTGGAAGGTAAGTTTAACGTGCAG
GAGGCGATCGCATGTTGGCAAAACGCATAA

Upstream 100 bases:

>100_bases
TTCTGGCAAGGCAGGCGCGTTATTTTTGCGTAATTTTGTGGAAAACATTAAATAAAGTGCGGTCGATTTTTTAAAAATTT
TGCAAACACAGGACAACACA

Downstream 100 bases:

>100_bases
TTCCTTGCTTAGACGTACGTGATGGGCAGGTGGTCAAAGGTGTACAATTTCGCAATCACGAGATTATTGGCGATATTGTG
CCGCTGGCGCAACGCTATGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKTSQIIPALDLIDGQVVRLYQGDYGQKTLYSDNPIAQFQDYVAQGAKYLHLVDLTGAKDPTKRQTQLIGEIINAVNCSI
QVGGGIRTEQDVADLLAVGANRVVIGSTAVKQPEMVKQWFKKYGAEKFVLALDVNINAQGEKQIAVSGWQENSGVSLEAL
IADFREVGLQHVLCTDISKDGTLQGSNVALYQEICTQFPTIQFQSSGGIGSIADIDALKGTGVAGVIVGRALLEGKFNVQ
EAIACWQNA

Sequences:

>Translated_249_residues
MKTSQIIPALDLIDGQVVRLYQGDYGQKTLYSDNPIAQFQDYVAQGAKYLHLVDLTGAKDPTKRQTQLIGEIINAVNCSI
QVGGGIRTEQDVADLLAVGANRVVIGSTAVKQPEMVKQWFKKYGAEKFVLALDVNINAQGEKQIAVSGWQENSGVSLEAL
IADFREVGLQHVLCTDISKDGTLQGSNVALYQEICTQFPTIQFQSSGGIGSIADIDALKGTGVAGVIVGRALLEGKFNVQ
EAIACWQNA
>Mature_249_residues
MKTSQIIPALDLIDGQVVRLYQGDYGQKTLYSDNPIAQFQDYVAQGAKYLHLVDLTGAKDPTKRQTQLIGEIINAVNCSI
QVGGGIRTEQDVADLLAVGANRVVIGSTAVKQPEMVKQWFKKYGAEKFVLALDVNINAQGEKQIAVSGWQENSGVSLEAL
IADFREVGLQHVLCTDISKDGTLQGSNVALYQEICTQFPTIQFQSSGGIGSIADIDALKGTGVAGVIVGRALLEGKFNVQ
EAIACWQNA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=67.6229508196721, Blast_Score=349, Evalue=8e-98,
Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=23.3463035019455, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_PASMU (Q9CLM1)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246140.1
- ProteinModelPortal:   Q9CLM1
- SMR:   Q9CLM1
- GeneID:   1244550
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1203
- NMPDR:   fig|272843.1.peg.1203
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   PMUL272843:PM1203-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26759; Mature: 26759

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSQIIPALDLIDGQVVRLYQGDYGQKTLYSDNPIAQFQDYVAQGAKYLHLVDLTGAKD
CCCCCCCCHHHHCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHCCCCEEEEEEECCCCC
PTKRQTQLIGEIINAVNCSIQVGGGIRTEQDVADLLAVGANRVVIGSTAVKQPEMVKQWF
CHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
KKYGAEKFVLALDVNINAQGEKQIAVSGWQENSGVSLEALIADFREVGLQHVLCTDISKD
HHCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
GTLQGSNVALYQEICTQFPTIQFQSSGGIGSIADIDALKGTGVAGVIVGRALLEGKFNVQ
CCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
EAIACWQNA
HHHHHHCCC
>Mature Secondary Structure
MKTSQIIPALDLIDGQVVRLYQGDYGQKTLYSDNPIAQFQDYVAQGAKYLHLVDLTGAKD
CCCCCCCCHHHHCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHCCCCEEEEEEECCCCC
PTKRQTQLIGEIINAVNCSIQVGGGIRTEQDVADLLAVGANRVVIGSTAVKQPEMVKQWF
CHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHH
KKYGAEKFVLALDVNINAQGEKQIAVSGWQENSGVSLEALIADFREVGLQHVLCTDISKD
HHCCCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
GTLQGSNVALYQEICTQFPTIQFQSSGGIGSIADIDALKGTGVAGVIVGRALLEGKFNVQ
CCCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
EAIACWQNA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100