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The map label for this gene is hisH

Identifier: 15603067

GI number: 15603067

Start: 1396267

End: 1396863

Strand: Direct

Name: hisH

Synonym: PM1202

Alternate gene names: 15603067

Gene position: 1396267-1396863 (Clockwise)

Preceding gene: 15603066

Following gene: 15603068

Centisome position: 61.85

GC content: 43.22

Gene sequence:

>597_bases
ATGCAAAATATAACTATAATCAACACCCGTTGCGCGAACCTTTCTTCCGTCAAATTTGCTTTTGACCGTTTAGGTTACCA
CACAGAAATTACCGATAATATCGAAAAAATCCAATCCGCCGACAAGCTGATTTTGCCTGGTGTGGGAACGGCAAAAGCAG
CGATGCAAAATTTGGCGGATTTAGGCTTAATTGAGGTTATCCAAAGCCTAACTCAACCTGTTTTGGGGATTTGTTTGGGA
ATGCAATTAATGACCGACTATTCCGAAGAAGGCAATTTGGACTTATTAAAACTGATGTCAGGCAAAACCGAAAAATTACC
GGATTGTCAGTTGCCGTTACCGCATATGGGCTGGAATAAGGTGCATTATGCCGCCGATCACCCGCTCTTTGCAGAGATTG
CTCAAGACAGCTATTTCTATTTTGTGCATAGCTACGGCGTGTTGCCCAATCCGCACACCGTTGCCACTTGCGACTATGGT
GTGCCATTTTCTGCGGTAATCCAACATAACAATTTTTATGGTGCACAATTCCACCCTGAGCGTTCTGGCAAGGCAGGCGC
GTTATTTTTGCGTAATTTTGTGGAAAACATTAAATAA

Upstream 100 bases:

>100_bases
TAGAAAAATATAAAGCTCTATCCAAACGGAAGAATGATATTTATTCTAGGTTTATTGAGTCAGTTAATATTGAATTTAAT
GTGATAGAAAAGATCTAAAT

Downstream 100 bases:

>100_bases
AGTGCGGTCGATTTTTTAAAAATTTTGCAAACACAGGACAACACAATGAAAACATCACAAATTATCCCCGCTCTTGATCT
GATTGATGGTCAAGTGGTGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MQNITIINTRCANLSSVKFAFDRLGYHTEITDNIEKIQSADKLILPGVGTAKAAMQNLADLGLIEVIQSLTQPVLGICLG
MQLMTDYSEEGNLDLLKLMSGKTEKLPDCQLPLPHMGWNKVHYAADHPLFAEIAQDSYFYFVHSYGVLPNPHTVATCDYG
VPFSAVIQHNNFYGAQFHPERSGKAGALFLRNFVENIK

Sequences:

>Translated_198_residues
MQNITIINTRCANLSSVKFAFDRLGYHTEITDNIEKIQSADKLILPGVGTAKAAMQNLADLGLIEVIQSLTQPVLGICLG
MQLMTDYSEEGNLDLLKLMSGKTEKLPDCQLPLPHMGWNKVHYAADHPLFAEIAQDSYFYFVHSYGVLPNPHTVATCDYG
VPFSAVIQHNNFYGAQFHPERSGKAGALFLRNFVENIK
>Mature_198_residues
MQNITIINTRCANLSSVKFAFDRLGYHTEITDNIEKIQSADKLILPGVGTAKAAMQNLADLGLIEVIQSLTQPVLGICLG
MQLMTDYSEEGNLDLLKLMSGKTEKLPDCQLPLPHMGWNKVHYAADHPLFAEIAQDSYFYFVHSYGVLPNPHTVATCDYG
VPFSAVIQHNNFYGAQFHPERSGKAGALFLRNFVENIK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=194, Percent_Identity=53.6082474226804, Blast_Score=215, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6319725, Length=212, Percent_Identity=30.188679245283, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_PASMU (P57921)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246139.1
- ProteinModelPortal:   P57921
- SMR:   P57921
- GeneID:   1244549
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1202
- NMPDR:   fig|272843.1.peg.1202
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13170
- BioCyc:   PMUL272843:PM1202-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR006220
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- PRINTS:   PR00097
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 21989; Mature: 21989

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 78-78 ACT_SITE 178-178 ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNITIINTRCANLSSVKFAFDRLGYHTEITDNIEKIQSADKLILPGVGTAKAAMQNLAD
CCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHC
LGLIEVIQSLTQPVLGICLGMQLMTDYSEEGNLDLLKLMSGKTEKLPDCQLPLPHMGWNK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCC
VHYAADHPLFAEIAQDSYFYFVHSYGVLPNPHTVATCDYGVPFSAVIQHNNFYGAQFHPE
EEECCCCCHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCHHHEEECCCEECCEECCC
RSGKAGALFLRNFVENIK
CCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNITIINTRCANLSSVKFAFDRLGYHTEITDNIEKIQSADKLILPGVGTAKAAMQNLAD
CCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHC
LGLIEVIQSLTQPVLGICLGMQLMTDYSEEGNLDLLKLMSGKTEKLPDCQLPLPHMGWNK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCCCC
VHYAADHPLFAEIAQDSYFYFVHSYGVLPNPHTVATCDYGVPFSAVIQHNNFYGAQFHPE
EEECCCCCHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCHHHEEECCCEECCEECCC
RSGKAGALFLRNFVENIK
CCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100