Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 15603069

GI number: 15603069

Start: 1397640

End: 1398410

Strand: Direct

Name: hisF

Synonym: PM1204

Alternate gene names: 15603069

Gene position: 1397640-1398410 (Clockwise)

Preceding gene: 15603068

Following gene: 15603070

Centisome position: 61.91

GC content: 45.27

Gene sequence:

>771_bases
ATGTTGGCAAAACGCATAATTCCTTGCTTAGACGTACGTGATGGGCAGGTGGTCAAAGGTGTACAATTTCGCAATCACGA
GATTATTGGCGATATTGTGCCGCTGGCGCAACGCTATGCGGAAGAAGGCGCAGATGAATTAGTGTTCTATGATATTACCG
CCTCCTCTGACGGCAGAACTATTGATAAAAGCTGGGTGGAACGTGTGGCACAAGTGATTGATATTCCATTCTGTGTCGCC
GGTGGGATTAAAAGCGTGGAAGATGCCGAAAAACTATTCGCCTTTGGCGCAGATAAAATTTCCATAAATTCACCCGCACT
TGCTGATCCCGATTTAATTAATCGCTTAGCGGATCGTTTTGGTGTGCAAGCCATTGTGGTGGGGATCGATAGCTGGTTTG
AAAAAGAAACCGGCAAATATTGGGTTAATCAATATACTGGCGATGAAAGTCGCACACGCCAAACCCATTGGCAATTACTC
GACTGGGTCAAGGAAGTACAACAACGTGGCGCGGGCGAAATTGTGTTAAATATGATGAACCAAGACGGTGTTCGTCAAGG
TTATGACATCGCCCAATTAAAACTCGTGCGTAACCTCTGTCATATTCCCCTGATTGCCTCAGGCGGTGCAGGTGAAATGG
TGCATTTCCGTGATGCCTTTATTGAAGCCAATGTGGATGGTGCGCTCGCTGCCAGCGTGTTCCATAAACGGATTATTGAT
ATTGGGGAATTAAAGGATTATTTGAGAAAGGAAAAGATTAAGATTAGATAG

Upstream 100 bases:

>100_bases
CCATCGCGGATATTGACGCCTTAAAAGGCACGGGGGTCGCTGGTGTGATTGTGGGGCGTGCTTTATTGGAAGGTAAGTTT
AACGTGCAGGAGGCGATCGC

Downstream 100 bases:

>100_bases
AAAATATTTAAACTAAATTAATAAGGTAAATTCATGAAAAAGTCTGAATTCAAAAATAGTTTTATAGAATTACTTAGTAA
TAATATTGATAACTTAAATT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRTIDKSWVERVAQVIDIPFCVA
GGIKSVEDAEKLFAFGADKISINSPALADPDLINRLADRFGVQAIVVGIDSWFEKETGKYWVNQYTGDESRTRQTHWQLL
DWVKEVQQRGAGEIVLNMMNQDGVRQGYDIAQLKLVRNLCHIPLIASGGAGEMVHFRDAFIEANVDGALAASVFHKRIID
IGELKDYLRKEKIKIR

Sequences:

>Translated_256_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRTIDKSWVERVAQVIDIPFCVA
GGIKSVEDAEKLFAFGADKISINSPALADPDLINRLADRFGVQAIVVGIDSWFEKETGKYWVNQYTGDESRTRQTHWQLL
DWVKEVQQRGAGEIVLNMMNQDGVRQGYDIAQLKLVRNLCHIPLIASGGAGEMVHFRDAFIEANVDGALAASVFHKRIID
IGELKDYLRKEKIKIR
>Mature_256_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRTIDKSWVERVAQVIDIPFCVA
GGIKSVEDAEKLFAFGADKISINSPALADPDLINRLADRFGVQAIVVGIDSWFEKETGKYWVNQYTGDESRTRQTHWQLL
DWVKEVQQRGAGEIVLNMMNQDGVRQGYDIAQLKLVRNLCHIPLIASGGAGEMVHFRDAFIEANVDGALAASVFHKRIID
IGELKDYLRKEKIKIR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=81.640625, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI87082028, Length=215, Percent_Identity=25.1162790697674, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319725, Length=317, Percent_Identity=32.4921135646688, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_PASMU (Q9CLM0)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246141.1
- ProteinModelPortal:   Q9CLM0
- SMR:   Q9CLM0
- GeneID:   1244551
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1204
- NMPDR:   fig|272843.1.peg.1204
- HOGENOM:   HBG541613
- OMA:   ASVFHKG
- ProtClustDB:   PRK02083
- BioCyc:   PMUL272843:PM1204-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28713; Mature: 28713

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRT
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
IDKSWVERVAQVIDIPFCVAGGIKSVEDAEKLFAFGADKISINSPALADPDLINRLADRF
CCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQAIVVGIDSWFEKETGKYWVNQYTGDESRTRQTHWQLLDWVKEVQQRGAGEIVLNMMN
CCEEEEEEHHHHHHHCCCCEEHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHC
QDGVRQGYDIAQLKLVRNLCHIPLIASGGAGEMVHFRDAFIEANVDGALAASVFHKRIID
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKDYLRKEKIKIR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAEEGADELVFYDITASSDGRT
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCC
IDKSWVERVAQVIDIPFCVAGGIKSVEDAEKLFAFGADKISINSPALADPDLINRLADRF
CCHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHH
GVQAIVVGIDSWFEKETGKYWVNQYTGDESRTRQTHWQLLDWVKEVQQRGAGEIVLNMMN
CCEEEEEEHHHHHHHCCCCEEHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHC
QDGVRQGYDIAQLKLVRNLCHIPLIASGGAGEMVHFRDAFIEANVDGALAASVFHKRIID
CCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCEEHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKDYLRKEKIKIR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100