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The map label for this gene is seqA [H]

Identifier: 15602221

GI number: 15602221

Start: 415664

End: 416284

Strand: Direct

Name: seqA [H]

Synonym: PM0356

Alternate gene names: 15602221

Gene position: 415664-416284 (Clockwise)

Preceding gene: 15602216

Following gene: 15602222

Centisome position: 18.41

GC content: 41.22

Gene sequence:

>621_bases
ATGAAAATTATTGAAGTTGATGAAGAGCTTTATCAATATATTGCGAGTCAAACGCAGTCTATTGGCGAAAGCGCATCGGA
TATTTTGCGCCGTTTGCTTAATTTTTCTAATAAAGCTGTTGAAAAAACAGAAGTGCCTCCCGTGGCGGTTAAAACGACAG
AGCCTGCACCAACAGCACCACAACCAGCCGTCCAAGCAGAAACAGAAGCGGTTTTTGAAAATCCAAAAATCTTACCTAAA
AAACAATCTGATGAGTCTATTCAGCAAGTGGTCACCAAAGTTCGTGACTTACTCCGTTCTGATGCATTTCAACAAGAAAG
TAAAGCGGTTGTACGTTTTTTAAATATTTTGAAAGTGCTTTATCGTACGAACCCAGAAAGTTTTGCGCAAGCAACTGAAT
CATTGCAAGGGCGTACCCGTGTTTATTTTGCGCGTGATGAAGGCACATTATTAATGGCGGGAAATCAAACGAAGCCAAAG
CAAATTCCCGATACGCCATATTGGGTGATCACCAATACCAATAGTGGTCGCAAAATGTTGATGCTAGAAGGCGCAATGCA
ATCGATGCATTTACCGGCGGACTTAATTGAAGAAGTGCGTAGCTATTTTGTTGCCAATTAG

Upstream 100 bases:

>100_bases
AAGTAAATTGTTCTCAGACATAATTTGTATATAGAGTATTTGTAAAGAAGTCGTTATAATGTTGCAAAATCTGAAAGAGA
TCAAGCAAGTGGAGGAAAAA

Downstream 100 bases:

>100_bases
TTATGTTTCCTTGGCAGCGATTCGCATCTGATCCTATTAAACAAAACCAAATCGCCTTGCGAGATCCGCAAGGCGAGGTT
TTTACATGGCGACAGCTTTT

Product: replication initiation regulator SeqA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKIIEVDEELYQYIASQTQSIGESASDILRRLLNFSNKAVEKTEVPPVAVKTTEPAPTAPQPAVQAETEAVFENPKILPK
KQSDESIQQVVTKVRDLLRSDAFQQESKAVVRFLNILKVLYRTNPESFAQATESLQGRTRVYFARDEGTLLMAGNQTKPK
QIPDTPYWVITNTNSGRKMLMLEGAMQSMHLPADLIEEVRSYFVAN

Sequences:

>Translated_206_residues
MKIIEVDEELYQYIASQTQSIGESASDILRRLLNFSNKAVEKTEVPPVAVKTTEPAPTAPQPAVQAETEAVFENPKILPK
KQSDESIQQVVTKVRDLLRSDAFQQESKAVVRFLNILKVLYRTNPESFAQATESLQGRTRVYFARDEGTLLMAGNQTKPK
QIPDTPYWVITNTNSGRKMLMLEGAMQSMHLPADLIEEVRSYFVAN
>Mature_206_residues
MKIIEVDEELYQYIASQTQSIGESASDILRRLLNFSNKAVEKTEVPPVAVKTTEPAPTAPQPAVQAETEAVFENPKILPK
KQSDESIQQVVTKVRDLLRSDAFQQESKAVVRFLNILKVLYRTNPESFAQATESLQGRTRVYFARDEGTLLMAGNQTKPK
QIPDTPYWVITNTNSGRKMLMLEGAMQSMHLPADLIEEVRSYFVAN

Specific function: Negative regulator of replication initiation. Required for sequestration, a process that blocks secondary initiation events. Could block the allosteric transition of dnaA from an inactive form to an active form [H]

COG id: COG3057

COG function: function code L; Negative regulator of replication initiationR

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786903, Length=199, Percent_Identity=51.7587939698493, Blast_Score=195, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005621
- InterPro:   IPR010985 [H]

Pfam domain/function: PF03925 SeqA [H]

EC number: NA

Molecular weight: Translated: 23190; Mature: 23190

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIEVDEELYQYIASQTQSIGESASDILRRLLNFSNKAVEKTEVPPVAVKTTEPAPTAP
CCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCC
QPAVQAETEAVFENPKILPKKQSDESIQQVVTKVRDLLRSDAFQQESKAVVRFLNILKVL
CCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRTNPESFAQATESLQGRTRVYFARDEGTLLMAGNQTKPKQIPDTPYWVITNTNSGRKML
HHCCHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCEEE
MLEGAMQSMHLPADLIEEVRSYFVAN
EEHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIIEVDEELYQYIASQTQSIGESASDILRRLLNFSNKAVEKTEVPPVAVKTTEPAPTAP
CCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCC
QPAVQAETEAVFENPKILPKKQSDESIQQVVTKVRDLLRSDAFQQESKAVVRFLNILKVL
CCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRTNPESFAQATESLQGRTRVYFARDEGTLLMAGNQTKPKQIPDTPYWVITNTNSGRKML
HHCCHHHHHHHHHHHCCCEEEEEEECCCEEEEECCCCCCCCCCCCCEEEEEECCCCCEEE
MLEGAMQSMHLPADLIEEVRSYFVAN
EEHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]