Click here to switch to the map view.

The map label for this gene is obgE

Identifier: 15602216

GI number: 15602216

Start: 412144

End: 413316

Strand: Direct

Name: obgE

Synonym: PM0351

Alternate gene names: 15602216

Gene position: 412144-413316 (Clockwise)

Preceding gene: 15602215

Following gene: 15602221

Centisome position: 18.26

GC content: 45.01

Gene sequence:

>1173_bases
ATGAAATTTATTGATGAAGCACTAATTCGTGTTGAAGCGGGTGATGGTGGTAACGGCTGTGTGAGCTTCCGACGTGAAAA
ATATATCCCTAAAGGCGGACCAGATGGTGGCGATGGAGGGGATGGGGGGGACGTGTATTTAGTTGCCGATGAAAACCTCA
ATACCTTAATTGACTATCGCTTTGAAAAACGCTTTGCTGCGGGACGTGGCGAAAACGGACGCAGTGCTGGTTGTACTGGA
CACCGTGGCAATGATATTACTTTAAGAGTGCCTGTGGGGACTCGTGCGATTGATAACGATACGAAAGAAGTCTTAGGTGA
CTTAACTAAACATGGTGCCAAAATGCTTGTGGCGAAAGGCGGTTACCATGGCTTAGGCAACACACGTTTTAAATCCTCCG
TTAACCGTGCACCTAGACAAAAAACCAATGGCACACCCGGAGAAAAGCGTGATTTGCAATTAGAGTTAATGTTGTTAGCC
GATGTCGGTATGCTAGGTTTACCGAATGCTGGAAAATCTACATTTATTCGAGCCGTTTCAGCGGCGAAACCCAAAGTAGC
GGATTATCCTTTTACGACATTAGTACCGAGTTTAGGCGTCGTGCGTGTTGATGAAAACCACAGTTTTGTGGTGGCAGATA
TCCCGGGATTAATTGAGGGAGCGGCTGAAGGCGCTGGTTTAGGTGTGCGTTTCTTAAAACATTTAGAGCGCTGTCGCGTC
TTAATTCATTTAGTTGATATTGCACCTATTGATGAGAGTGATCCTGCAGAGAATATCTCGATCATTGAATCAGAATTATT
TCAATACAGTGAAGCCCTGGCGGATAAGCCACGTTGGTTAGTGTTTAATAAAATTGACACCATGAGTGATGAAGAGGCAC
ACGAGCGTGCGCAAGCGATTACTGAACGCTTAGGCTGGGATGACGACTATTACCTCATTTCTGCAGTAACAGGCAAAAAT
GTACAGCCACTTTGTCGTGATATTATGGACTTTATTGAAGCAAATCCACGTCACGAAGTTGAGCAAACAGCGGACGAGGC
AGAAGTGAAATTTAAATGGGATGATTACCACCAAGCACAATTAGCGGATCATCAGTTCGAGGATGAAGACGAGGATTGGG
ATGATTGGTCAGAAGAAGACGAAGAAGGGGTAGAAACCATTTACAAGCCGTAA

Upstream 100 bases:

>100_bases
CAGATTTAAATTCAATCAGTTATATTGGTGCGCTGATAGTGGTTTTAGGGGCAATTACATCCGCTATTGGGCACAAATTT
ATTAAGCGTTAGGAGAAAGA

Downstream 100 bases:

>100_bases
AGTTGACAATTGACAGGCTACTAAAAAAGTGCGGTATAAACGTTGAAAATTTATACCGCACTTTTTATTTAATTCGCCTT
GTTTTTATTTTTTGCCATTC

Product: GTPase ObgE

Products: NA

Alternate protein names: GTP-binding protein obg

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKFIDEALIRVEAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGDVYLVADENLNTLIDYRFEKRFAAGRGENGRSAGCTG
HRGNDITLRVPVGTRAIDNDTKEVLGDLTKHGAKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLQLELMLLA
DVGMLGLPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRVDENHSFVVADIPGLIEGAAEGAGLGVRFLKHLERCRV
LIHLVDIAPIDESDPAENISIIESELFQYSEALADKPRWLVFNKIDTMSDEEAHERAQAITERLGWDDDYYLISAVTGKN
VQPLCRDIMDFIEANPRHEVEQTADEAEVKFKWDDYHQAQLADHQFEDEDEDWDDWSEEDEEGVETIYKP

Sequences:

>Translated_390_residues
MKFIDEALIRVEAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGDVYLVADENLNTLIDYRFEKRFAAGRGENGRSAGCTG
HRGNDITLRVPVGTRAIDNDTKEVLGDLTKHGAKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLQLELMLLA
DVGMLGLPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRVDENHSFVVADIPGLIEGAAEGAGLGVRFLKHLERCRV
LIHLVDIAPIDESDPAENISIIESELFQYSEALADKPRWLVFNKIDTMSDEEAHERAQAITERLGWDDDYYLISAVTGKN
VQPLCRDIMDFIEANPRHEVEQTADEAEVKFKWDDYHQAQLADHQFEDEDEDWDDWSEEDEEGVETIYKP
>Mature_390_residues
MKFIDEALIRVEAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGDVYLVADENLNTLIDYRFEKRFAAGRGENGRSAGCTG
HRGNDITLRVPVGTRAIDNDTKEVLGDLTKHGAKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLQLELMLLA
DVGMLGLPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRVDENHSFVVADIPGLIEGAAEGAGLGVRFLKHLERCRV
LIHLVDIAPIDESDPAENISIIESELFQYSEALADKPRWLVFNKIDTMSDEEAHERAQAITERLGWDDDYYLISAVTGKN
VQPLCRDIMDFIEANPRHEVEQTADEAEVKFKWDDYHQAQLADHQFEDEDEDWDDWSEEDEEGVETIYKP

Specific function: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in t

COG id: COG0536

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI24308117, Length=340, Percent_Identity=39.4117647058824, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI111955139, Length=388, Percent_Identity=35.0515463917526, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI111955063, Length=180, Percent_Identity=38.8888888888889, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI58761500, Length=128, Percent_Identity=35.9375, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI4758796, Length=127, Percent_Identity=33.0708661417323, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789574, Length=389, Percent_Identity=73.0077120822622, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1787454, Length=136, Percent_Identity=34.5588235294118, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508313, Length=295, Percent_Identity=41.0169491525424, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17552324, Length=309, Percent_Identity=32.0388349514563, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17509631, Length=169, Percent_Identity=33.7278106508876, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321962, Length=161, Percent_Identity=36.6459627329193, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319281, Length=108, Percent_Identity=34.2592592592593, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24585318, Length=301, Percent_Identity=39.5348837209302, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI20129375, Length=326, Percent_Identity=37.7300613496933, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24640873, Length=128, Percent_Identity=34.375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640877, Length=128, Percent_Identity=34.375, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640875, Length=128, Percent_Identity=34.375, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OBG_PASMU (Q9CNS4)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245288.1
- HSSP:   P20964
- ProteinModelPortal:   Q9CNS4
- SMR:   Q9CNS4
- GeneID:   1243698
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0351
- NMPDR:   fig|272843.1.peg.351
- HOGENOM:   HBG716038
- OMA:   LIDYRFN
- ProtClustDB:   PRK12298
- BioCyc:   PMUL272843:PM0351-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01454
- InterPro:   IPR014100
- InterPro:   IPR006074
- InterPro:   IPR006073
- InterPro:   IPR006169
- InterPro:   IPR002917
- Gene3D:   G3DSA:2.70.210.12
- PANTHER:   PTHR11702:SF3
- PIRSF:   PIRSF002401
- PRINTS:   PR00326
- TIGRFAMs:   TIGR02729

Pfam domain/function: PF01018 GTP1_OBG; PF01926 MMR_HSR1; SSF82051 GTP1_OBG_sub

EC number: NA

Molecular weight: Translated: 43120; Mature: 43120

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00905 GTP1_OBG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIDEALIRVEAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGDVYLVADENLNTLIDYR
CCCHHEEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
FEKRFAAGRGENGRSAGCTGHRGNDITLRVPVGTRAIDNDTKEVLGDLTKHGAKMLVAKG
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECC
GYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLQLELMLLADVGMLGLPNAGKSTFIRAVS
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
AAKPKVADYPFTTLVPSLGVVRVDENHSFVVADIPGLIEGAAEGAGLGVRFLKHLERCRV
HCCCCCCCCCHHHHHCCCCEEEECCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHH
LIHLVDIAPIDESDPAENISIIESELFQYSEALADKPRWLVFNKIDTMSDEEAHERAQAI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHH
TERLGWDDDYYLISAVTGKNVQPLCRDIMDFIEANPRHEVEQTADEAEVKFKWDDYHQAQ
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCHHHH
LADHQFEDEDEDWDDWSEEDEEGVETIYKP
HHCCCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKFIDEALIRVEAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGDVYLVADENLNTLIDYR
CCCHHEEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
FEKRFAAGRGENGRSAGCTGHRGNDITLRVPVGTRAIDNDTKEVLGDLTKHGAKMLVAKG
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECC
GYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLQLELMLLADVGMLGLPNAGKSTFIRAVS
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHH
AAKPKVADYPFTTLVPSLGVVRVDENHSFVVADIPGLIEGAAEGAGLGVRFLKHLERCRV
HCCCCCCCCCHHHHHCCCCEEEECCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHH
LIHLVDIAPIDESDPAENISIIESELFQYSEALADKPRWLVFNKIDTMSDEEAHERAQAI
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHH
TERLGWDDDYYLISAVTGKNVQPLCRDIMDFIEANPRHEVEQTADEAEVKFKWDDYHQAQ
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCHHHH
LADHQFEDEDEDWDDWSEEDEEGVETIYKP
HHCCCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100