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The map label for this gene is menE [H]

Identifier: 15602222

GI number: 15602222

Start: 416287

End: 417705

Strand: Direct

Name: menE [H]

Synonym: PM0357

Alternate gene names: 15602222

Gene position: 416287-417705 (Clockwise)

Preceding gene: 15602221

Following gene: 15602223

Centisome position: 18.44

GC content: 43.13

Gene sequence:

>1419_bases
ATGTTTCCTTGGCAGCGATTCGCATCTGATCCTATTAAACAAAACCAAATCGCCTTGCGAGATCCGCAAGGCGAGGTTTT
TACATGGCGACAGCTTTTTGAGCAAATCAGCCAGTATGCTAAGCTTTTGCAATACCATGGCGTTCAACCTAATTCCGGTG
TCGCTTTATGTGGTAAGAATGAACTGGAATTGTTATTACTCTACTTAGCCACTATTCAGATTGGCGCACGAGTCTTTCCG
CTTAATCCTGCTTTTCCTGTCGAAAAAATTCAGCAATATTGCCGTGCGGCAGATATTGAGTTTTATTATGCACCACATGC
CTTACTCTTACGTGACTGCCAAGCGATTTCGTTAACAGTGAATGTTCAAGTGGAACAAGTTGAGCCAGAAGTGCGGTCAG
ATTGTGAAAACTTTTTTATTCCCGCTACTATGACTTTAACCTCTGGGTCTAGTAGTGTTCCTAAAGCGGTTGTTCATCAC
CTACAAGCGCATTTAGATAATGCCAAAGGCGTTTGTGAACTCATGCAGTTTGACGCAAATGCGTCATGGTTACTTTCTTT
GCCTTTATATCATGTTTCAGGGCAGGGGATCCTTTGGCGTTGGTTACGTGCAGGCGCAGTCTTACATTTTCCACAAGCGG
ACTTTTATGCGTCTGTCGGGCAAGCGACACATGTTTCTTTAGTCCCGACTCAATTACAACGTTTATTATCTTATTGGCAG
GAAAATACGATTTTAGACAGCTCGTTAAAAACCCAGCATATTTTGCTCGGTGGGGCGCAGATTCCGACGGCATTAACTGC
ACAATTAGCAAAATGGGGCGTGCAAAGTTATTCGGGATATGGCATGACGGAAATGGCGTCGACCGTATTTGCGAAACGAA
GTGATGATAAAATGGGCGTTGGTCAACCGTTATGTGGACGTGAATATCGACTCGTTGAGGGCGAAATCTGGCTGAAAGGT
GCGGGATTAGCGCTTGGTTATTGGCAACAGGGAACGATTTTACCGCTCACCAATAAACAAGGTTGGTTTGCCACAAAAGA
TAAAGGGATATGGCGTGATAATGAGTTGGTAATTTTAGGACGAATTGATAATATGTTCATTTCCGGTGGCGAAAACATAC
AGCCCGAAGAAATCGAACGGATAATTCAGCAATGGCAATATGTCAAACAAGTGTTTGTGTTGCCGAAAGCGGATATTGAG
TTTGGACAACGCCCCGTGGCGTTCATCGAATTTTCTGTGCCTTTTTCTGACGCACTTGTTGAGGCATTGACGCTTTGGCT
TGCGGATAAATTGGAAAAATTTAAACACCCCGTGGCTTATTTCCCTTTGAATACACAACAATTACAACAAGGTGCAATTA
AAATTTCTCGAACAAGATTAAAATTAGCCTTACAGGATTTACTAGGAACTCACGAATGA

Upstream 100 bases:

>100_bases
GTGGTCGCAAAATGTTGATGCTAGAAGGCGCAATGCAATCGATGCATTTACCGGCGGACTTAATTGAAGAAGTGCGTAGC
TATTTTGTTGCCAATTAGTT

Downstream 100 bases:

>100_bases
AAAAAACACATTTAGTTAAAAAGGCCTTTTTTGCCCTGAGCTTATGTTTTATGTTCAACGCATACACATTCGCTCAACTT
CCTACACCAAATGATATTCA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MFPWQRFASDPIKQNQIALRDPQGEVFTWRQLFEQISQYAKLLQYHGVQPNSGVALCGKNELELLLLYLATIQIGARVFP
LNPAFPVEKIQQYCRAADIEFYYAPHALLLRDCQAISLTVNVQVEQVEPEVRSDCENFFIPATMTLTSGSSSVPKAVVHH
LQAHLDNAKGVCELMQFDANASWLLSLPLYHVSGQGILWRWLRAGAVLHFPQADFYASVGQATHVSLVPTQLQRLLSYWQ
ENTILDSSLKTQHILLGGAQIPTALTAQLAKWGVQSYSGYGMTEMASTVFAKRSDDKMGVGQPLCGREYRLVEGEIWLKG
AGLALGYWQQGTILPLTNKQGWFATKDKGIWRDNELVILGRIDNMFISGGENIQPEEIERIIQQWQYVKQVFVLPKADIE
FGQRPVAFIEFSVPFSDALVEALTLWLADKLEKFKHPVAYFPLNTQQLQQGAIKISRTRLKLALQDLLGTHE

Sequences:

>Translated_472_residues
MFPWQRFASDPIKQNQIALRDPQGEVFTWRQLFEQISQYAKLLQYHGVQPNSGVALCGKNELELLLLYLATIQIGARVFP
LNPAFPVEKIQQYCRAADIEFYYAPHALLLRDCQAISLTVNVQVEQVEPEVRSDCENFFIPATMTLTSGSSSVPKAVVHH
LQAHLDNAKGVCELMQFDANASWLLSLPLYHVSGQGILWRWLRAGAVLHFPQADFYASVGQATHVSLVPTQLQRLLSYWQ
ENTILDSSLKTQHILLGGAQIPTALTAQLAKWGVQSYSGYGMTEMASTVFAKRSDDKMGVGQPLCGREYRLVEGEIWLKG
AGLALGYWQQGTILPLTNKQGWFATKDKGIWRDNELVILGRIDNMFISGGENIQPEEIERIIQQWQYVKQVFVLPKADIE
FGQRPVAFIEFSVPFSDALVEALTLWLADKLEKFKHPVAYFPLNTQQLQQGAIKISRTRLKLALQDLLGTHE
>Mature_472_residues
MFPWQRFASDPIKQNQIALRDPQGEVFTWRQLFEQISQYAKLLQYHGVQPNSGVALCGKNELELLLLYLATIQIGARVFP
LNPAFPVEKIQQYCRAADIEFYYAPHALLLRDCQAISLTVNVQVEQVEPEVRSDCENFFIPATMTLTSGSSSVPKAVVHH
LQAHLDNAKGVCELMQFDANASWLLSLPLYHVSGQGILWRWLRAGAVLHFPQADFYASVGQATHVSLVPTQLQRLLSYWQ
ENTILDSSLKTQHILLGGAQIPTALTAQLAKWGVQSYSGYGMTEMASTVFAKRSDDKMGVGQPLCGREYRLVEGEIWLKG
AGLALGYWQQGTILPLTNKQGWFATKDKGIWRDNELVILGRIDNMFISGGENIQPEEIERIIQQWQYVKQVFVLPKADIE
FGQRPVAFIEFSVPFSDALVEALTLWLADKLEKFKHPVAYFPLNTQQLQQGAIKISRTRLKLALQDLLGTHE

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=461, Percent_Identity=41.648590021692, Blast_Score=327, Evalue=7e-91,
Organism=Escherichia coli, GI145693145, Length=478, Percent_Identity=22.5941422594142, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788107, Length=469, Percent_Identity=22.6012793176972, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=461, Percent_Identity=27.765726681128, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=442, Percent_Identity=26.4705882352941, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=400, Percent_Identity=24.25, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=468, Percent_Identity=21.7948717948718, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 53245; Mature: 53245

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPWQRFASDPIKQNQIALRDPQGEVFTWRQLFEQISQYAKLLQYHGVQPNSGVALCGKN
CCCCHHHCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
ELELLLLYLATIQIGARVFPLNPAFPVEKIQQYCRAADIEFYYAPHALLLRDCQAISLTV
CHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCEEEECCHHHHHHCCCEEEEEE
NVQVEQVEPEVRSDCENFFIPATMTLTSGSSSVPKAVVHHLQAHLDNAKGVCELMQFDAN
EEEEEECCCHHHCCHHHEEECEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCC
ASWLLSLPLYHVSGQGILWRWLRAGAVLHFPQADFYASVGQATHVSLVPTQLQRLLSYWQ
CEEEEEECEEEECCCHHHHHHHHCCCEEECCCHHHHHHCCCCCEEEECHHHHHHHHHHHH
ENTILDSSLKTQHILLGGAQIPTALTAQLAKWGVQSYSGYGMTEMASTVFAKRSDDKMGV
CCCEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
GQPLCGREYRLVEGEIWLKGAGLALGYWQQGTILPLTNKQGWFATKDKGIWRDNELVILG
CCCCCCCCEEEEECEEEEEECCEEEEEECCCEEEEECCCCCCEEECCCCCCCCCCEEEEE
RIDNMFISGGENIQPEEIERIIQQWQYVKQVFVLPKADIEFGQRPVAFIEFSVPFSDALV
ECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHH
EALTLWLADKLEKFKHPVAYFPLNTQQLQQGAIKISRTRLKLALQDLLGTHE
HHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFPWQRFASDPIKQNQIALRDPQGEVFTWRQLFEQISQYAKLLQYHGVQPNSGVALCGKN
CCCCHHHCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
ELELLLLYLATIQIGARVFPLNPAFPVEKIQQYCRAADIEFYYAPHALLLRDCQAISLTV
CHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCEEEECCHHHHHHCCCEEEEEE
NVQVEQVEPEVRSDCENFFIPATMTLTSGSSSVPKAVVHHLQAHLDNAKGVCELMQFDAN
EEEEEECCCHHHCCHHHEEECEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCC
ASWLLSLPLYHVSGQGILWRWLRAGAVLHFPQADFYASVGQATHVSLVPTQLQRLLSYWQ
CEEEEEECEEEECCCHHHHHHHHCCCEEECCCHHHHHHCCCCCEEEECHHHHHHHHHHHH
ENTILDSSLKTQHILLGGAQIPTALTAQLAKWGVQSYSGYGMTEMASTVFAKRSDDKMGV
CCCEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
GQPLCGREYRLVEGEIWLKGAGLALGYWQQGTILPLTNKQGWFATKDKGIWRDNELVILG
CCCCCCCCEEEEECEEEEEECCEEEEEECCCEEEEECCCCCCEEECCCCCCCCCCEEEEE
RIDNMFISGGENIQPEEIERIIQQWQYVKQVFVLPKADIEFGQRPVAFIEFSVPFSDALV
ECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHH
EALTLWLADKLEKFKHPVAYFPLNTQQLQQGAIKISRTRLKLALQDLLGTHE
HHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]