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The map label for this gene is murF [H]

Identifier: 15602003

GI number: 15602003

Start: 163092

End: 164474

Strand: Direct

Name: murF [H]

Synonym: PM0138

Alternate gene names: 15602003

Gene position: 163092-164474 (Clockwise)

Preceding gene: 15602002

Following gene: 15602004

Centisome position: 7.22

GC content: 41.72

Gene sequence:

>1383_bases
ATGCTTAAATTAACAACAAAACAACTGGCAACGATTTTACAAGCCGAATTGGTGGGCGAGGCAAATGTTGTTGTAGAGAA
TATCAGTACAGATACGCGTCAGCGTGTTGCCAGTTCACTCTTTTTTGCCTTAAAAGGTGAACATTTTGATGCGCATAATT
ATTTAGATAAAGCCGTCGCACAAGGGGCAAACGCCCTTGTGGTTGAGCGTGCGGATACGCAAATTTCAGTACCGCAGTTG
ATCGTTAAGGATACACGCATTGCACTTGGTGAACTTGCCAAATGGGTGAAACAGCAAGTGAATCCACGCACGCTAGCGAT
GACAGGATCATCCGGCAAAACCACAGTAAAAGAAATGACCGCTAGCATTCTACAAAGTGCGGTCGGTGTTGAGCAGGTTT
TATTTACACAAGGTAATTTTAATAATGATATTGGTGTGCCTTTAACCTTATTGCGTTTAACCGAGCAACATCAATTTGCG
GTGATTGAATTAGGCGCAAATCATCAAGGTGAAATCGCTTATACCACGGGGTTAGTACAACCGAATGTGGCATTAGTGAA
TAATGTGGCTGCTGCTCATCTAGAAGGCTTTGGCAGTCTTGAAGGTGTGGCAACAGCCAAAGGTGAAATTTATCGTGGTT
TACAGCCGGATGGTGTGGCGATTATCAATAAAGAGCATGATTATCGCAGTACGTGGGCGCAGGATATTGGTGCTCACAGG
GTACAATATTTCGCTTATCAGGATAACACAGCCGATTTTTATGCCGATAATGTTATATTTCATGAAGCGGGATCGACTTT
TGATTTATGTACACCACAAGGGAAAACCACGATTCATCTCCCTTATTTGGGGGAACATAATGTCAAAAATGCTTTAGCTG
CCTCTGCGTTAGCCATGAATGTGGGGGCGACCTTAGCGCAAATCAAACAAGGTTTAGAAGTTCGCACACAAGTGAAAGGG
CGGTTATATCCAGTACAACCTTGTGACAATCTCTTATTACTTGATGATACTTATAATGCCAATGTGGATTCATTACAATC
AGCGATTCGTGTTCTACAAAAATATGACGCATTTCGAATTTTAGTTGTTGGAGATATGGCAGAATTAGGCGAAAATAGCC
AAATTTGTCATCAACAGGTGGCGGAGTGTGCGAAAGCGGCGAAATTAGAGAGGGTGTACTCGTTTGGCAGCGAAAGTGCG
GTGATTTCTCAAGCATGTTTAGGTAAACATTTTACGGATAAAGCGTTGTTGTGCCGTGAACTAACGCAACTGTTGAGTGA
AAAATTAAAAGAAAACCAAAAAATCGTTTTATTAGCAAAAGGATCACGTCGAATGCAAATGGAGGATGTGATTAATACTT
TAAGGGAAAACTTTGTATGTTAG

Upstream 100 bases:

>100_bases
GCGGGAAAAGGGCATGAAGACTATCAAATTATCGGCACACAGAAACAGCATTTCTCTGATCAAGAAACCGTACAACAGTA
TTTTAAGGTAAATCATCACC

Downstream 100 bases:

>100_bases
TTTGGCTCGGTGAGTTTTTACAACAATATTACAGCGGATTTAATGTGATCTCTTATATCACAGTACGTGCCATTTTAGCG
TTATTAACCGCACTTTTGGT

Product: hypothetical protein

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MLKLTTKQLATILQAELVGEANVVVENISTDTRQRVASSLFFALKGEHFDAHNYLDKAVAQGANALVVERADTQISVPQL
IVKDTRIALGELAKWVKQQVNPRTLAMTGSSGKTTVKEMTASILQSAVGVEQVLFTQGNFNNDIGVPLTLLRLTEQHQFA
VIELGANHQGEIAYTTGLVQPNVALVNNVAAAHLEGFGSLEGVATAKGEIYRGLQPDGVAIINKEHDYRSTWAQDIGAHR
VQYFAYQDNTADFYADNVIFHEAGSTFDLCTPQGKTTIHLPYLGEHNVKNALAASALAMNVGATLAQIKQGLEVRTQVKG
RLYPVQPCDNLLLLDDTYNANVDSLQSAIRVLQKYDAFRILVVGDMAELGENSQICHQQVAECAKAAKLERVYSFGSESA
VISQACLGKHFTDKALLCRELTQLLSEKLKENQKIVLLAKGSRRMQMEDVINTLRENFVC

Sequences:

>Translated_460_residues
MLKLTTKQLATILQAELVGEANVVVENISTDTRQRVASSLFFALKGEHFDAHNYLDKAVAQGANALVVERADTQISVPQL
IVKDTRIALGELAKWVKQQVNPRTLAMTGSSGKTTVKEMTASILQSAVGVEQVLFTQGNFNNDIGVPLTLLRLTEQHQFA
VIELGANHQGEIAYTTGLVQPNVALVNNVAAAHLEGFGSLEGVATAKGEIYRGLQPDGVAIINKEHDYRSTWAQDIGAHR
VQYFAYQDNTADFYADNVIFHEAGSTFDLCTPQGKTTIHLPYLGEHNVKNALAASALAMNVGATLAQIKQGLEVRTQVKG
RLYPVQPCDNLLLLDDTYNANVDSLQSAIRVLQKYDAFRILVVGDMAELGENSQICHQQVAECAKAAKLERVYSFGSESA
VISQACLGKHFTDKALLCRELTQLLSEKLKENQKIVLLAKGSRRMQMEDVINTLRENFVC
>Mature_460_residues
MLKLTTKQLATILQAELVGEANVVVENISTDTRQRVASSLFFALKGEHFDAHNYLDKAVAQGANALVVERADTQISVPQL
IVKDTRIALGELAKWVKQQVNPRTLAMTGSSGKTTVKEMTASILQSAVGVEQVLFTQGNFNNDIGVPLTLLRLTEQHQFA
VIELGANHQGEIAYTTGLVQPNVALVNNVAAAHLEGFGSLEGVATAKGEIYRGLQPDGVAIINKEHDYRSTWAQDIGAHR
VQYFAYQDNTADFYADNVIFHEAGSTFDLCTPQGKTTIHLPYLGEHNVKNALAASALAMNVGATLAQIKQGLEVRTQVKG
RLYPVQPCDNLLLLDDTYNANVDSLQSAIRVLQKYDAFRILVVGDMAELGENSQICHQQVAECAKAAKLERVYSFGSESA
VISQACLGKHFTDKALLCRELTQLLSEKLKENQKIVLLAKGSRRMQMEDVINTLRENFVC

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=461, Percent_Identity=53.1453362255965, Blast_Score=438, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50354; Mature: 50354

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLTTKQLATILQAELVGEANVVVENISTDTRQRVASSLFFALKGEHFDAHNYLDKAVA
CCEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
QGANALVVERADTQISVPQLIVKDTRIALGELAKWVKQQVNPRTLAMTGSSGKTTVKEMT
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ASILQSAVGVEQVLFTQGNFNNDIGVPLTLLRLTEQHQFAVIELGANHQGEIAYTTGLVQ
HHHHHHHHCHHHHHEECCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCEEEEECCCC
PNVALVNNVAAAHLEGFGSLEGVATAKGEIYRGLQPDGVAIINKEHDYRSTWAQDIGAHR
CCHHHHHHHHHHHHCCCCCCCCCEECCCHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCE
VQYFAYQDNTADFYADNVIFHEAGSTFDLCTPQGKTTIHLPYLGEHNVKNALAASALAMN
EEEEEEECCCCCCEECCEEEECCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHH
VGATLAQIKQGLEVRTQVKGRLYPVQPCDNLLLLDDTYNANVDSLQSAIRVLQKYDAFRI
HHHHHHHHHCCCHHHHHHCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
LVVGDMAELGENSQICHQQVAECAKAAKLERVYSFGSESAVISQACLGKHFTDKALLCRE
EEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHH
LTQLLSEKLKENQKIVLLAKGSRRMQMEDVINTLRENFVC
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKLTTKQLATILQAELVGEANVVVENISTDTRQRVASSLFFALKGEHFDAHNYLDKAVA
CCEECHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
QGANALVVERADTQISVPQLIVKDTRIALGELAKWVKQQVNPRTLAMTGSSGKTTVKEMT
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
ASILQSAVGVEQVLFTQGNFNNDIGVPLTLLRLTEQHQFAVIELGANHQGEIAYTTGLVQ
HHHHHHHHCHHHHHEECCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCCEEEEECCCC
PNVALVNNVAAAHLEGFGSLEGVATAKGEIYRGLQPDGVAIINKEHDYRSTWAQDIGAHR
CCHHHHHHHHHHHHCCCCCCCCCEECCCHHHCCCCCCCEEEEECCCCHHHHHHHHHCCCE
VQYFAYQDNTADFYADNVIFHEAGSTFDLCTPQGKTTIHLPYLGEHNVKNALAASALAMN
EEEEEEECCCCCCEECCEEEECCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHH
VGATLAQIKQGLEVRTQVKGRLYPVQPCDNLLLLDDTYNANVDSLQSAIRVLQKYDAFRI
HHHHHHHHHCCCHHHHHHCCCEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEE
LVVGDMAELGENSQICHQQVAECAKAAKLERVYSFGSESAVISQACLGKHFTDKALLCRE
EEEECHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHH
LTQLLSEKLKENQKIVLLAKGSRRMQMEDVINTLRENFVC
HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]