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The map label for this gene is murE

Identifier: 15602002

GI number: 15602002

Start: 161615

End: 163099

Strand: Direct

Name: murE

Synonym: PM0137

Alternate gene names: 15602002

Gene position: 161615-163099 (Clockwise)

Preceding gene: 15602001

Following gene: 15602003

Centisome position: 7.16

GC content: 44.65

Gene sequence:

>1485_bases
ATGCGAAGATTAACCGCACTTTTGGGGCAAGTTGAACTTGCCCCAAATATTGAATTGACGGAAATGATTCTAGACAGTCG
GGCTGTAAAGCAAGGCTGTCTTTTTGTTGCATTACAGGGGCACCAAGTTGATGGACGTCAGTATATTCCGCAAGCCATCG
CCAAGGGGGCAAGTGCGGTGTTAGCTGAAACAGAGGATGCACAGCAGCATTTGAGCACGAAAATAGAACAAGGGGTGCCG
ATCATTTCGTTTTATCAATTAGGCTCTCACCTTTCTGCGTTAGCGGGGCTTTTTTATGATAATCCGTCGCACAAATTGAC
CTTGGTTGGGGTGACGGGCACGAATGGCAAAACTACTATTTCGCAATTATTAGCGCAATGGACAACATTACTGGGGCATC
GCAGTGCTGTGATGGGAACGATTGGCAATGGGTTGTTGGGGCAAGTGAAAGAAGCAACCAATACCACTGGATCTGCGGTG
GAAGTTCAAGCTTCTTTAGCGGATTTTGTTAAGCGAGGTGCAGATTTCGCCGCGATTGAGGTTTCATCACATGGACTAGT
GCAGCACCGCGTTGAAGCGTTAGCCTTTGATGTTGCTATTTTTACTAATCTCAGCCGTGATCATTTGGATTATCATCAAA
GTATGGAAAATTATGCGTTGGCCAAAAAACGCTTATTTACCGAATTGAATTCCCGACACCAAATTATTAATGCAGACGAT
AGCGTCGGACAAACTTGGTTACAGGAACAACCCAATGCAGTAGCCGTCAGCTGTCAGACAGATTTTAAACCTCATCAAGC
TCGTTGGTTGAAAGCAACTGCGATTCAGTTTAGTCAGAAAGGTGCACGAATTCAATTTGAATCAAGTTGGGGAAAAGGCG
AATTACATAGTGCGTTGATAGGGCAGTTTAATGTTAGTAATTTGCTGTTGGTGTTTGCTACCTTACTTTCATTGGGCTAT
GACATTGAAAAACTCATGAAGACGGTGCCTCAATTAACGGGGGTATGTGGTCGGATGGAAAGATTAAGTGCATCAAACCA
ACCTACCGCGATTGTGGATTATGCCCATACGCCAGATGCGTTAGAGAAAGCTTTGCAAGCAGCGCGATTACATTGTCAGG
GGAAATTGTGGTGTGTGTTTGGTTGTGGTGGTGATCGTGATCGTGGTAAACGTCCTATTATGGCGAAAATTGCGGAGCAA
TTTGCTGATCACGTGATTGTGACAGATGATAATCCGCGTACTGAATCCGCGGCGCAAATTGTGCAAGATATTCTGGCTGG
ATTTGAACATCCGCAACACGTTGAGGTCTGCCACGCTCGAGATCAAGCGATTATTCAGGCATTACAAAAAGCAGATAGCG
ATGATGTCGTGCTGATTGCGGGAAAAGGGCATGAAGACTATCAAATTATCGGCACACAGAAACAGCATTTCTCTGATCAA
GAAACCGTACAACAGTATTTTAAGGTAAATCATCACCATGCTTAA

Upstream 100 bases:

>100_bases
GAATAATATTCCACCAGATGCGATGCCACAAGATAAAATGGCAAGACGCGTTGTGCGTTTGAATAGTGATAATGGAACAA
CGACAAACTAAGAAGAAATT

Downstream 100 bases:

>100_bases
ATTAACAACAAAACAACTGGCAACGATTTTACAAGCCGAATTGGTGGGCGAGGCAAATGTTGTTGTAGAGAATATCAGTA
CAGATACGCGTCAGCGTGTT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MRRLTALLGQVELAPNIELTEMILDSRAVKQGCLFVALQGHQVDGRQYIPQAIAKGASAVLAETEDAQQHLSTKIEQGVP
IISFYQLGSHLSALAGLFYDNPSHKLTLVGVTGTNGKTTISQLLAQWTTLLGHRSAVMGTIGNGLLGQVKEATNTTGSAV
EVQASLADFVKRGADFAAIEVSSHGLVQHRVEALAFDVAIFTNLSRDHLDYHQSMENYALAKKRLFTELNSRHQIINADD
SVGQTWLQEQPNAVAVSCQTDFKPHQARWLKATAIQFSQKGARIQFESSWGKGELHSALIGQFNVSNLLLVFATLLSLGY
DIEKLMKTVPQLTGVCGRMERLSASNQPTAIVDYAHTPDALEKALQAARLHCQGKLWCVFGCGGDRDRGKRPIMAKIAEQ
FADHVIVTDDNPRTESAAQIVQDILAGFEHPQHVEVCHARDQAIIQALQKADSDDVVLIAGKGHEDYQIIGTQKQHFSDQ
ETVQQYFKVNHHHA

Sequences:

>Translated_494_residues
MRRLTALLGQVELAPNIELTEMILDSRAVKQGCLFVALQGHQVDGRQYIPQAIAKGASAVLAETEDAQQHLSTKIEQGVP
IISFYQLGSHLSALAGLFYDNPSHKLTLVGVTGTNGKTTISQLLAQWTTLLGHRSAVMGTIGNGLLGQVKEATNTTGSAV
EVQASLADFVKRGADFAAIEVSSHGLVQHRVEALAFDVAIFTNLSRDHLDYHQSMENYALAKKRLFTELNSRHQIINADD
SVGQTWLQEQPNAVAVSCQTDFKPHQARWLKATAIQFSQKGARIQFESSWGKGELHSALIGQFNVSNLLLVFATLLSLGY
DIEKLMKTVPQLTGVCGRMERLSASNQPTAIVDYAHTPDALEKALQAARLHCQGKLWCVFGCGGDRDRGKRPIMAKIAEQ
FADHVIVTDDNPRTESAAQIVQDILAGFEHPQHVEVCHARDQAIIQALQKADSDDVVLIAGKGHEDYQIIGTQKQHFSDQ
ETVQQYFKVNHHHA
>Mature_494_residues
MRRLTALLGQVELAPNIELTEMILDSRAVKQGCLFVALQGHQVDGRQYIPQAIAKGASAVLAETEDAQQHLSTKIEQGVP
IISFYQLGSHLSALAGLFYDNPSHKLTLVGVTGTNGKTTISQLLAQWTTLLGHRSAVMGTIGNGLLGQVKEATNTTGSAV
EVQASLADFVKRGADFAAIEVSSHGLVQHRVEALAFDVAIFTNLSRDHLDYHQSMENYALAKKRLFTELNSRHQIINADD
SVGQTWLQEQPNAVAVSCQTDFKPHQARWLKATAIQFSQKGARIQFESSWGKGELHSALIGQFNVSNLLLVFATLLSLGY
DIEKLMKTVPQLTGVCGRMERLSASNQPTAIVDYAHTPDALEKALQAARLHCQGKLWCVFGCGGDRDRGKRPIMAKIAEQ
FADHVIVTDDNPRTESAAQIVQDILAGFEHPQHVEVCHARDQAIIQALQKADSDDVVLIAGKGHEDYQIIGTQKQHFSDQ
ETVQQYFKVNHHHA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=490, Percent_Identity=59.1836734693878, Blast_Score=536, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_PASMU (P57815)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245074.1
- ProteinModelPortal:   P57815
- SMR:   P57815
- GeneID:   1243484
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0137
- NMPDR:   fig|272843.1.peg.137
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   PRK00139
- BioCyc:   PMUL272843:PM0137-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54172; Mature: 54172

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 26-26 BINDING 154-154 BINDING 182-182 BINDING 188-188 BINDING 190-190 BINDING 386-386 BINDING 461-461 BINDING 465-465

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLTALLGQVELAPNIELTEMILDSRAVKQGCLFVALQGHQVDGRQYIPQAIAKGASAV
CCHHHHHHHCEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCHHH
LAETEDAQQHLSTKIEQGVPIISFYQLGSHLSALAGLFYDNPSHKLTLVGVTGTNGKTTI
HHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHH
SQLLAQWTTLLGHRSAVMGTIGNGLLGQVKEATNTTGSAVEVQASLADFVKRGADFAAIE
HHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCEEEEE
VSSHGLVQHRVEALAFDVAIFTNLSRDHLDYHQSMENYALAKKRLFTELNSRHQIINADD
ECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
SVGQTWLQEQPNAVAVSCQTDFKPHQARWLKATAIQFSQKGARIQFESSWGKGELHSALI
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
GQFNVSNLLLVFATLLSLGYDIEKLMKTVPQLTGVCGRMERLSASNQPTAIVDYAHTPDA
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHH
LEKALQAARLHCQGKLWCVFGCGGDRDRGKRPIMAKIAEQFADHVIVTDDNPRTESAAQI
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCHHHHHH
VQDILAGFEHPQHVEVCHARDQAIIQALQKADSDDVVLIAGKGHEDYQIIGTQKQHFSDQ
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHCCCH
ETVQQYFKVNHHHA
HHHHHHHHHCCCCC
>Mature Secondary Structure
MRRLTALLGQVELAPNIELTEMILDSRAVKQGCLFVALQGHQVDGRQYIPQAIAKGASAV
CCHHHHHHHCEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCHHH
LAETEDAQQHLSTKIEQGVPIISFYQLGSHLSALAGLFYDNPSHKLTLVGVTGTNGKTTI
HHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHH
SQLLAQWTTLLGHRSAVMGTIGNGLLGQVKEATNTTGSAVEVQASLADFVKRGADFAAIE
HHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCEEEEE
VSSHGLVQHRVEALAFDVAIFTNLSRDHLDYHQSMENYALAKKRLFTELNSRHQIINADD
ECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
SVGQTWLQEQPNAVAVSCQTDFKPHQARWLKATAIQFSQKGARIQFESSWGKGELHSALI
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
GQFNVSNLLLVFATLLSLGYDIEKLMKTVPQLTGVCGRMERLSASNQPTAIVDYAHTPDA
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHH
LEKALQAARLHCQGKLWCVFGCGGDRDRGKRPIMAKIAEQFADHVIVTDDNPRTESAAQI
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCHHHHHH
VQDILAGFEHPQHVEVCHARDQAIIQALQKADSDDVVLIAGKGHEDYQIIGTQKQHFSDQ
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCHHHCCCH
ETVQQYFKVNHHHA
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100