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The map label for this gene is proS

Identifier: 15674109

GI number: 15674109

Start: 2196129

End: 2197979

Strand: Reverse

Name: proS

Synonym: L0356

Alternate gene names: 15674109

Gene position: 2197979-2196129 (Counterclockwise)

Preceding gene: 15674110

Following gene: 15674108

Centisome position: 92.91

GC content: 36.68

Gene sequence:

>1851_bases
ATGAAACAATCAAAAATGCTTATACCAACCCTTCGGGAAATGCCTTCTGATGCCCAGGTTATCAGCCATGCCTTACTTAT
GCGCGCAGGATATGTTCGTCAAGTTTCAGCAGGAATTTATGCTTACTTGCCATTAGCAAACCGCGTACTTGAAAAGTTGA
AAAATATCATGCGTGAAGAGTTTGACGAAATTGGTGCAGTAGAACTTCTTGCCCCAAGTCTTTTGACAGCTGACCTTTGG
CGTGAATCTGGCCGTTATGACACATATGGTGAGGATTTATATAAATTAAAAAACCGTGATAATTCTGATTTTATTTTAGG
ACCAACACATGAAGAAACAATGACTTCTCTTGTCCGTGATGAAATTACTTCTTATAAAAAATTACCACTTAATGTTTACC
AAATTGCTCCAAAATTCCGTGATGAAAAACGTCCACGTTATGGACTATTGCGCGGTCGTGAGTTCTTGATGAAAGATGGT
TATAGCTACCATGCAGATCAAGATTCATTGGACGAGACTTATAATGATTACAAAAAAGCCTACGAAAAAATCTTTGAACG
TGCTGGCTTAAACTTTAAACCAATTATTGCCGATGCTGGAGCAATGGGTGGTAAAGATAGTCAAGAATTTATTGCTATTA
CAGATGATAGAATCAATCTTGAAAAATGGCTCGTTTTGAGTAAGAATATTACATCAATCGAAGAAATTCCAGAATCTGTC
CTTTCAGAAATCAAAGAAGAACTTGGCAAATGGTTGATTGCTGGTGAAGATACAATTGTCTACGCTGAAGGTGGTGATTA
TGCGGCAAATATTGAAATGGCCAGCAGTCAATTTGAACCAAATGTAGCTTATACTGAAGAACTTGAACTTGAAAAAGTAG
CGACACCAGGTGCTAAAACAATTGATGAAGTTTCAAAATTCCTTGAAATTGATGAAGAACAAACAGTTAAAACATTGGTT
TATCACGCAGATGATGAATTAGTGGTTGTTTTGTTAAATGGTAATGACCAACTCAACGAAGTCAAATTGACCAATTATCT
TGGAGCATCATTTATTGAAGCTGCCAGTGAAGCAGAAGTTGAAGAAAAATTTGGAGCTCATTTTGGTTCACTTGGACCAA
TCGGGCTTGAAAATGTCAAGATTATTGCTGACCGAAAAGTTGAACAAATTAAAAATGCTGTGGTTGGAGCTAACGTTGAT
GGTTTCCATTATAAAAATGCTAACTTTGGCCGTGATTATGAAGTTGAAGAGTTTGTTGATTTACGTACAGTTAATGAAGG
TGAAATTTCACCAGATGGCCGTGGAACTTTGAAATTTGCGCGTGGTATCGAAATTGGACATATCTTTAAATTAGGAACTC
GCTATACAGAAGCGATGAATGCCAATATTCTTGATGCGAATGGTCGTTCAATTCCAATGCTCATGGGTTGTTACGGAATC
GGTGTAAGTCGTTTGCTTTCAGCTATTTTGGAACAATTTGCTCGTATCTATGTTGAAAAAACGCCAAGAGAAGAATTTAA
ATTTAGTTGGTCAATTAATTTCCCTAAAGAATTAGCACCATTTGATATTCATCTTGTTCCAGTTAATGTTAAAGATGAAG
CAGCAATGGAATTGACTTCTGAACTTGAAGAAAAATTGCGTGGCAAAGGTTATCAGGTCTTAGTAGATGACCGTAATGAA
CGTGCAGGTGTTAAATTTGCTGACAGCGATTTGATTGGTTTGCCAGTTCGTGTGACAATCGGTAAAAAAGCTGCTGAGGG
TGTTGTTGAAGTTAAAATTCGTGCGACAGGTGAAGTTGTTGAAATTAACAAAGATGAACTTGTCAATACTATTGAAATTT
TAAGTAAATAA

Upstream 100 bases:

>100_bases
GAATGATATTCTCCGAGCCTTTGTAAATTAATATATCTATTCGTCTTCTATTTCAAAAGGAAAGAAGCTGAACCAAACTC
ATAAAGGAAATTTTAAAATT

Downstream 100 bases:

>100_bases
TAAAAGAGATTTACTATGAAAATTGTTATTGTTGGAAGCTCCCACGCCGGAATTTGTGCCGGATTACGGGCGCTTGAAGA
ATACCCAGAAGCCGAAATTA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREEFDEIGAVELLAPSLLTADLW
RESGRYDTYGEDLYKLKNRDNSDFILGPTHEETMTSLVRDEITSYKKLPLNVYQIAPKFRDEKRPRYGLLRGREFLMKDG
YSYHADQDSLDETYNDYKKAYEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIEEIPESV
LSEIKEELGKWLIAGEDTIVYAEGGDYAANIEMASSQFEPNVAYTEELELEKVATPGAKTIDEVSKFLEIDEEQTVKTLV
YHADDELVVVLLNGNDQLNEVKLTNYLGASFIEAASEAEVEEKFGAHFGSLGPIGLENVKIIADRKVEQIKNAVVGANVD
GFHYKNANFGRDYEVEEFVDLRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTSELEEKLRGKGYQVLVDDRNE
RAGVKFADSDLIGLPVRVTIGKKAAEGVVEVKIRATGEVVEINKDELVNTIEILSK

Sequences:

>Translated_616_residues
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREEFDEIGAVELLAPSLLTADLW
RESGRYDTYGEDLYKLKNRDNSDFILGPTHEETMTSLVRDEITSYKKLPLNVYQIAPKFRDEKRPRYGLLRGREFLMKDG
YSYHADQDSLDETYNDYKKAYEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIEEIPESV
LSEIKEELGKWLIAGEDTIVYAEGGDYAANIEMASSQFEPNVAYTEELELEKVATPGAKTIDEVSKFLEIDEEQTVKTLV
YHADDELVVVLLNGNDQLNEVKLTNYLGASFIEAASEAEVEEKFGAHFGSLGPIGLENVKIIADRKVEQIKNAVVGANVD
GFHYKNANFGRDYEVEEFVDLRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTSELEEKLRGKGYQVLVDDRNE
RAGVKFADSDLIGLPVRVTIGKKAAEGVVEVKIRATGEVVEINKDELVNTIEILSK
>Mature_616_residues
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREEFDEIGAVELLAPSLLTADLW
RESGRYDTYGEDLYKLKNRDNSDFILGPTHEETMTSLVRDEITSYKKLPLNVYQIAPKFRDEKRPRYGLLRGREFLMKDG
YSYHADQDSLDETYNDYKKAYEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIEEIPESV
LSEIKEELGKWLIAGEDTIVYAEGGDYAANIEMASSQFEPNVAYTEELELEKVATPGAKTIDEVSKFLEIDEEQTVKTLV
YHADDELVVVLLNGNDQLNEVKLTNYLGASFIEAASEAEVEEKFGAHFGSLGPIGLENVKIIADRKVEQIKNAVVGANVD
GFHYKNANFGRDYEVEEFVDLRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTSELEEKLRGKGYQVLVDDRNE
RAGVKFADSDLIGLPVRVTIGKKAAEGVVEVKIRATGEVVEINKDELVNTIEILSK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=37.5545851528384, Blast_Score=175, Evalue=1e-43,
Organism=Escherichia coli, GI1786392, Length=617, Percent_Identity=41.9773095623987, Blast_Score=469, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI115532348, Length=198, Percent_Identity=36.8686868686869, Blast_Score=145, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6320931, Length=587, Percent_Identity=27.0868824531516, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24656200, Length=217, Percent_Identity=35.0230414746544, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_LACLA (Q9CDT4)

Other databases:

- EMBL:   AE005176
- PIR:   G86890
- RefSeq:   NP_268284.1
- ProteinModelPortal:   Q9CDT4
- SMR:   Q9CDT4
- GeneID:   1115804
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0356
- NMPDR:   fig|272623.1.peg.2182
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   LLAC272623:L0356-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 69251; Mature: 69251

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREE
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
FDEIGAVELLAPSLLTADLWRESGRYDTYGEDLYKLKNRDNSDFILGPTHEETMTSLVRD
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
EITSYKKLPLNVYQIAPKFRDEKRPRYGLLRGREFLMKDGYSYHADQDSLDETYNDYKKA
HHHHHHCCCCCCEECCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
YEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIEEIPESV
HHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHHEEECCCCHHHHHHHHHH
LSEIKEELGKWLIAGEDTIVYAEGGDYAANIEMASSQFEPNVAYTEELELEKVATPGAKT
HHHHHHHHCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCHH
IDEVSKFLEIDEEQTVKTLVYHADDELVVVLLNGNDQLNEVKLTNYLGASFIEAASEAEV
HHHHHHHHHCCCHHHEEEHEEECCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHH
EEKFGAHFGSLGPIGLENVKIIADRKVEQIKNAVVGANVDGFHYKNANFGRDYEVEEFVD
HHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHH
LRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
HEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTS
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHH
ELEEKLRGKGYQVLVDDRNERAGVKFADSDLIGLPVRVTIGKKAAEGVVEVKIRATGEVV
HHHHHHCCCCCEEEEECCCCCCCCEEECCCEECCEEEEEECCCCCCCEEEEEEEECCCEE
EINKDELVNTIEILSK
ECCHHHHHHHHHHHCC
>Mature Secondary Structure
MKQSKMLIPTLREMPSDAQVISHALLMRAGYVRQVSAGIYAYLPLANRVLEKLKNIMREE
CCCCCCCCHHHHHCCCHHHHHHHHHHHHHCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
FDEIGAVELLAPSLLTADLWRESGRYDTYGEDLYKLKNRDNSDFILGPTHEETMTSLVRD
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
EITSYKKLPLNVYQIAPKFRDEKRPRYGLLRGREFLMKDGYSYHADQDSLDETYNDYKKA
HHHHHHCCCCCCEECCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
YEKIFERAGLNFKPIIADAGAMGGKDSQEFIAITDDRINLEKWLVLSKNITSIEEIPESV
HHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCHHHHEEECCCCHHHHHHHHHH
LSEIKEELGKWLIAGEDTIVYAEGGDYAANIEMASSQFEPNVAYTEELELEKVATPGAKT
HHHHHHHHCCEEEECCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCHHHHCCCCCHH
IDEVSKFLEIDEEQTVKTLVYHADDELVVVLLNGNDQLNEVKLTNYLGASFIEAASEAEV
HHHHHHHHHCCCHHHEEEHEEECCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHH
EEKFGAHFGSLGPIGLENVKIIADRKVEQIKNAVVGANVDGFHYKNANFGRDYEVEEFVD
HHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHH
LRTVNEGEISPDGRGTLKFARGIEIGHIFKLGTRYTEAMNANILDANGRSIPMLMGCYGI
HEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
GVSRLLSAILEQFARIYVEKTPREEFKFSWSINFPKELAPFDIHLVPVNVKDEAAMELTS
HHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCHHHHHHHH
ELEEKLRGKGYQVLVDDRNERAGVKFADSDLIGLPVRVTIGKKAAEGVVEVKIRATGEVV
HHHHHHCCCCCEEEEECCCCCCCCEEECCCEECCEEEEEECCCCCCCEEEEEEEECCCEE
EINKDELVNTIEILSK
ECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471