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The map label for this gene is leuC

Identifier: 15673202

GI number: 15673202

Start: 1242879

End: 1244261

Strand: Direct

Name: leuC

Synonym: L0075

Alternate gene names: 15673202

Gene position: 1242879-1244261 (Clockwise)

Preceding gene: 15673200

Following gene: 15673203

Centisome position: 52.54

GC content: 39.91

Gene sequence:

>1383_bases
ATGTCAGGTAAAACAATATTTGATAAACTTTGGGATCAGCATGTGATTGCTGGAAATGAGGGAGAACCTCAACTGCTTTA
TATTGACCTTCATGTTATTCATGAGGTTACGAGTCCGCAAGCATTTCAGGGCTTACGTGAAGCAGGACGTCGTGTTCGGA
GAAAAGATTTGACATACGGAACTCTTGACCACAATGTTCCAACACAAGATATTTTTAATATTCAAGATTTGATTTCTAAA
AAACAAATTGATACTTTTACTAAAAATGTCAAAGAATTTGATGTTCCAGCGGAGACTCATGGTGGAAAAGGACAAGGAAT
TGTTCACATGGTAGCACCTGAATCTGGCAGAACTCAACCGGGAAAAACAATTGTTTGTGGCGATAGTCATACCGCAACAA
ATGGAGCATTTGGTGCAATTGCTTTTGGAATTGGTACAAGTGAAGTTGAACATGTTCTTGCAACTCAAACCATTTGGCAA
GTTAAACCCAAGCGTATGAAAATTGAATTTCAAGGTCATCCACAAAAAGGAATTTATAGCAAAGACTTTATCCTCGCATT
AATTGCTAAATATGGTGTGGATGCAGGTGTAGGTTATGCGGTTGAATATAGTGGGGATGCTATCAGTGATTTAAGCATGG
AAGAACGGATGACAATCTGTAACATGTCAATTGAATTTGGGGCAAAAATTGGCCTGATGAATCCTGATGAAAAAACTTAT
GACTATGTCAAAGGGCGTGAACATGCACCTAAAAACTTTGATGAAGCTGTCAGTAAATGGGAAAAACTTGTCAGTGATTC
TGATGCACAATACGATAAGATTTTAAGTCTTGATGTCAGCCAGTTGAAACCAATGGTGACATGGGGAACAAATCCCGGAA
TGGGCCTAGAATTTGGCGAAAAGTTTCCGGAAATTAACAATGATTTGAATTATGAACGTGCTTATCAGTACATGGATTTA
AAGCCAGGCCAAACTGCTTCTGCTATTGATTTAGGCTATATTTTCATTGGTTCTTGTACGAATGCTAGACTTGGTGATTT
AGAAGAAGCAGCAAAAATTATTGGAGACAGACATATTGCTGATGGACTGACAGGAATTGTCGTCCCTGGAAGCAGACCTG
TGAAAGAAGCGGCTGAAGCACAAGGTCTTGATAAAATTTTTAAAGAAGCTGGTTTTGAATGGCGGGAACCGGGTTGCTCA
GCCTGTCTTGGAATGAATCCTGACCAAATTCCAGAATACGTTCATTGTGCTTCAACCTCTAATCGAAATTTTGAAGGACG
TCAAGGACATAATGCAAGAACGCACCTGTGCTCTCCAGCTATGGCCGCTGCCGCCGCAATCGCTGGTAAATTTGTAGATG
TTAGAACGCTCGTAACAGATTAG

Upstream 100 bases:

>100_bases
AGTTGTAAATAAAAGAACTATAAGCGTAACTGACAATTCTGTCAGTAAATATTACTGACAGAATTTGTCAGAATAAATTT
TTAAAAAAGGAAATAAAAAA

Downstream 100 bases:

>100_bases
TCTGTAGAAAGAAAAAAAGATGGAAAAATTCACGATTTACAAAGGGACAAGTGTTCCAGTCATGAACGATAATATTGACA
CAGACCAAATTATTCCTAAA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSGKTIFDKLWDQHVIAGNEGEPQLLYIDLHVIHEVTSPQAFQGLREAGRRVRRKDLTYGTLDHNVPTQDIFNIQDLISK
KQIDTFTKNVKEFDVPAETHGGKGQGIVHMVAPESGRTQPGKTIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQ
VKPKRMKIEFQGHPQKGIYSKDFILALIAKYGVDAGVGYAVEYSGDAISDLSMEERMTICNMSIEFGAKIGLMNPDEKTY
DYVKGREHAPKNFDEAVSKWEKLVSDSDAQYDKILSLDVSQLKPMVTWGTNPGMGLEFGEKFPEINNDLNYERAYQYMDL
KPGQTASAIDLGYIFIGSCTNARLGDLEEAAKIIGDRHIADGLTGIVVPGSRPVKEAAEAQGLDKIFKEAGFEWREPGCS
ACLGMNPDQIPEYVHCASTSNRNFEGRQGHNARTHLCSPAMAAAAAIAGKFVDVRTLVTD

Sequences:

>Translated_460_residues
MSGKTIFDKLWDQHVIAGNEGEPQLLYIDLHVIHEVTSPQAFQGLREAGRRVRRKDLTYGTLDHNVPTQDIFNIQDLISK
KQIDTFTKNVKEFDVPAETHGGKGQGIVHMVAPESGRTQPGKTIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQ
VKPKRMKIEFQGHPQKGIYSKDFILALIAKYGVDAGVGYAVEYSGDAISDLSMEERMTICNMSIEFGAKIGLMNPDEKTY
DYVKGREHAPKNFDEAVSKWEKLVSDSDAQYDKILSLDVSQLKPMVTWGTNPGMGLEFGEKFPEINNDLNYERAYQYMDL
KPGQTASAIDLGYIFIGSCTNARLGDLEEAAKIIGDRHIADGLTGIVVPGSRPVKEAAEAQGLDKIFKEAGFEWREPGCS
ACLGMNPDQIPEYVHCASTSNRNFEGRQGHNARTHLCSPAMAAAAAIAGKFVDVRTLVTD
>Mature_459_residues
SGKTIFDKLWDQHVIAGNEGEPQLLYIDLHVIHEVTSPQAFQGLREAGRRVRRKDLTYGTLDHNVPTQDIFNIQDLISKK
QIDTFTKNVKEFDVPAETHGGKGQGIVHMVAPESGRTQPGKTIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQV
KPKRMKIEFQGHPQKGIYSKDFILALIAKYGVDAGVGYAVEYSGDAISDLSMEERMTICNMSIEFGAKIGLMNPDEKTYD
YVKGREHAPKNFDEAVSKWEKLVSDSDAQYDKILSLDVSQLKPMVTWGTNPGMGLEFGEKFPEINNDLNYERAYQYMDLK
PGQTASAIDLGYIFIGSCTNARLGDLEEAAKIIGDRHIADGLTGIVVPGSRPVKEAAEAQGLDKIFKEAGFEWREPGCSA
CLGMNPDQIPEYVHCASTSNRNFEGRQGHNARTHLCSPAMAAAAAIAGKFVDVRTLVTD

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI8659555, Length=446, Percent_Identity=25.7847533632287, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI4501867, Length=360, Percent_Identity=28.0555555555556, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI41352693, Length=374, Percent_Identity=25.1336898395722, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=55.1724137931034, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=26.6483516483516, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081781, Length=344, Percent_Identity=23.8372093023256, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17568399, Length=450, Percent_Identity=28, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25149337, Length=362, Percent_Identity=29.2817679558011, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564738, Length=362, Percent_Identity=29.2817679558011, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=307, Percent_Identity=28.9902280130293, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=468, Percent_Identity=51.7094017094017, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320440, Length=414, Percent_Identity=27.2946859903382, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=362, Percent_Identity=30.1104972375691, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=359, Percent_Identity=29.5264623955432, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281365315, Length=361, Percent_Identity=27.4238227146814, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17864292, Length=361, Percent_Identity=27.4238227146814, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161076999, Length=358, Percent_Identity=27.6536312849162, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28571643, Length=358, Percent_Identity=28.2122905027933, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137564, Length=462, Percent_Identity=25.5411255411255, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24645686, Length=458, Percent_Identity=25.5458515283843, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_LACLA (Q02142)

Other databases:

- EMBL:   U92974
- EMBL:   AE005176
- PIR:   D86777
- PIR:   S35134
- RefSeq:   NP_267376.1
- ProteinModelPortal:   Q02142
- GeneID:   1114869
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0075
- NMPDR:   fig|272623.1.peg.1255
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   LLAC272623:L0075-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50492; Mature: 50360

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKTIFDKLWDQHVIAGNEGEPQLLYIDLHVIHEVTSPQAFQGLREAGRRVRRKDLTYG
CCCCHHHHHHCCCEEEECCCCCCEEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHCCCEE
TLDHNVPTQDIFNIQDLISKKQIDTFTKNVKEFDVPAETHGGKGQGIVHMVAPESGRTQP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCC
GKTIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQVKPKRMKIEFQGHPQKGIYS
CCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCC
KDFILALIAKYGVDAGVGYAVEYSGDAISDLSMEERMTICNMSIEFGAKIGLMNPDEKTY
HHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHHHEEEEEECCCEECCCCCCHHHH
DYVKGREHAPKNFDEAVSKWEKLVSDSDAQYDKILSLDVSQLKPMVTWGTNPGMGLEFGE
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHCCCEEEECCCCCCCCHHHH
KFPEINNDLNYERAYQYMDLKPGQTASAIDLGYIFIGSCTNARLGDLEEAAKIIGDRHIA
HCCCCCCCCCHHHHHHEECCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHCCHHHH
DGLTGIVVPGSRPVKEAAEAQGLDKIFKEAGFEWREPGCSACLGMNPDQIPEYVHCASTS
CCCCEEEECCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHEEECCCC
NRNFEGRQGHNARTHLCSPAMAAAAAIAGKFVDVRTLVTD
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure 
SGKTIFDKLWDQHVIAGNEGEPQLLYIDLHVIHEVTSPQAFQGLREAGRRVRRKDLTYG
CCCHHHHHHCCCEEEECCCCCCEEEEEEEEEEHHCCCCHHHHHHHHHHHHHHHHCCCEE
TLDHNVPTQDIFNIQDLISKKQIDTFTKNVKEFDVPAETHGGKGQGIVHMVAPESGRTQP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCC
GKTIVCGDSHTATNGAFGAIAFGIGTSEVEHVLATQTIWQVKPKRMKIEFQGHPQKGIYS
CCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCC
KDFILALIAKYGVDAGVGYAVEYSGDAISDLSMEERMTICNMSIEFGAKIGLMNPDEKTY
HHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHHHEEEEEECCCEECCCCCCHHHH
DYVKGREHAPKNFDEAVSKWEKLVSDSDAQYDKILSLDVSQLKPMVTWGTNPGMGLEFGE
HHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCHHHCCCEEEECCCCCCCCHHHH
KFPEINNDLNYERAYQYMDLKPGQTASAIDLGYIFIGSCTNARLGDLEEAAKIIGDRHIA
HCCCCCCCCCHHHHHHEECCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHCCHHHH
DGLTGIVVPGSRPVKEAAEAQGLDKIFKEAGFEWREPGCSACLGMNPDQIPEYVHCASTS
CCCCEEEECCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCEECCCCHHHHHHHEEECCCC
NRNFEGRQGHNARTHLCSPAMAAAAAIAGKFVDVRTLVTD
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471