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The map label for this gene is leuD

Identifier: 15673203

GI number: 15673203

Start: 1244281

End: 1244856

Strand: Direct

Name: leuD

Synonym: L0076

Alternate gene names: 15673203

Gene position: 1244281-1244856 (Clockwise)

Preceding gene: 15673202

Following gene: 30024028

Centisome position: 52.6

GC content: 32.64

Gene sequence:

>576_bases
ATGGAAAAATTCACGATTTACAAAGGGACAAGTGTTCCAGTCATGAACGATAATATTGACACAGACCAAATTATTCCTAA
ACAATTTTTGAAAGCAATCGATAAAAAGGGCTTTGGGAAAAATTTATTTTATGAATGGCGTTATCTTAAAGATTACGATG
AGAATCCTGATTTTATTTTGAATGCTCCAAAATACAAAAAAGCTTCTCTGTTAATTTCAGGAGATAATTTTGGTTCGGGT
TCTTCAAGAGAACATGCGGCATGGGCCTTATCAGATTACGGCTTTCGGGCAATTATTGCTGGCTCTTACTCAGATATTTT
TTATAATAATGCTTTAAAAAATGGCTTGTTACCAATTAAACAACCAAGAGAAGTTCTAAATCAACTGACAAAACTGTCAA
GTCAAGAAGAAATTACAATTGATTTACCCCATCAGCTAATCATCACAAGCCTTGGTGACTTTCATTTTGAGATTGACCCC
ATTTGGAAAGATAAATTAATTAATGGCTTAGATGATATTGGAATAACTTTGCAATATGAAGAAGCAATCTCAGCTTACGA
ACAAAAAAATCAATAA

Upstream 100 bases:

>100_bases
CACCTGTGCTCTCCAGCTATGGCCGCTGCCGCCGCAATCGCTGGTAAATTTGTAGATGTTAGAACGCTCGTAACAGATTA
GTCTGTAGAAAGAAAAAAAG

Downstream 100 bases:

>100_bases
GAGCGAGCCTAAAATGACAATTATTAATTTAAAGAATATAAATCTTACTCGAAATAAAAAAGAAATTCTTAAAGATATTA
CTTGGAAAGTAAATCCCGGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MEKFTIYKGTSVPVMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYLKDYDENPDFILNAPKYKKASLLISGDNFGSG
SSREHAAWALSDYGFRAIIAGSYSDIFYNNALKNGLLPIKQPREVLNQLTKLSSQEEITIDLPHQLIITSLGDFHFEIDP
IWKDKLINGLDDIGITLQYEEAISAYEQKNQ

Sequences:

>Translated_191_residues
MEKFTIYKGTSVPVMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYLKDYDENPDFILNAPKYKKASLLISGDNFGSG
SSREHAAWALSDYGFRAIIAGSYSDIFYNNALKNGLLPIKQPREVLNQLTKLSSQEEITIDLPHQLIITSLGDFHFEIDP
IWKDKLINGLDDIGITLQYEEAISAYEQKNQ
>Mature_191_residues
MEKFTIYKGTSVPVMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYLKDYDENPDFILNAPKYKKASLLISGDNFGSG
SSREHAAWALSDYGFRAIIAGSYSDIFYNNALKNGLLPIKQPREVLNQLTKLSSQEEITIDLPHQLIITSLGDFHFEIDP
IWKDKLINGLDDIGITLQYEEAISAYEQKNQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=193, Percent_Identity=46.6321243523316, Blast_Score=183, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6321429, Length=196, Percent_Identity=52.0408163265306, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_LACLA (Q02144)

Other databases:

- EMBL:   U92974
- EMBL:   AE005176
- PIR:   E36889
- RefSeq:   NP_267377.1
- ProteinModelPortal:   Q02144
- SMR:   Q02144
- GeneID:   1114870
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0076
- NMPDR:   fig|272623.1.peg.1256
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   LLAC272623:L0076-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21849; Mature: 21849

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTIYKGTSVPVMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYLKDYDENPDFIL
CCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEE
NAPKYKKASLLISGDNFGSGSSREHAAWALSDYGFRAIIAGSYSDIFYNNALKNGLLPIK
ECCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCC
QPREVLNQLTKLSSQEEITIDLPHQLIITSLGDFHFEIDPIWKDKLINGLDDIGITLQYE
CHHHHHHHHHHCCCCCEEEEECCHHEEEECCCCCEEEECCCHHHHHHCCCCCCCEEEEHH
EAISAYEQKNQ
HHHHHHHHCCC
>Mature Secondary Structure
MEKFTIYKGTSVPVMNDNIDTDQIIPKQFLKAIDKKGFGKNLFYEWRYLKDYDENPDFIL
CCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEE
NAPKYKKASLLISGDNFGSGSSREHAAWALSDYGFRAIIAGSYSDIFYNNALKNGLLPIK
ECCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCC
QPREVLNQLTKLSSQEEITIDLPHQLIITSLGDFHFEIDPIWKDKLINGLDDIGITLQYE
CHHHHHHHHHHCCCCCEEEEECCHHEEEECCCCCEEEECCCHHHHHHCCCCCCCEEEEHH
EAISAYEQKNQ
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471