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The map label for this gene is leuB

Identifier: 15673200

GI number: 15673200

Start: 1241356

End: 1242318

Strand: Direct

Name: leuB

Synonym: L0074

Alternate gene names: 15673200

Gene position: 1241356-1242318 (Clockwise)

Preceding gene: 15673198

Following gene: 15673202

Centisome position: 52.48

GC content: 38.63

Gene sequence:

>963_bases
TTGTCTAAAAAAATTGTGACACTTGCGGGAGATGGAATTGGGCCAGAAATTATGTCAGCTGGTTTAAGTGTTTTAAAAGC
TGTCAGTAAAAAAATTGATTTTGAGTATGAATTAGAAGCTAAAGATTTTGGAGGAATTGCAATTGATAAGCATGGTCATC
CTTTACCAGAAGAAACTTTGCAAGCAGTTAAAAATGCTGACGCAATCTTGCTCGCTGCAATTGGTCATCCTAAATACAAC
AATGCAAAAGTTAGACCAGAACAAGGGCTACTTGCTTTACGAAAAGAATTAGGACTGTATGCTAATGTTCGTCCATTAAA
AATTTATCCGGCTCTAAAAAAACTTTCTCCTATCCGAAATGTTGAAAATGTTGATTTCCTAATGATTCGCGAACTGACAG
GGGGAATCTATTTCGGTCAGCATGAATTGGCAGATGACAAAGCACGGGATGTCAATGATTATTCTGCTGATGAAATAAGA
AGAATTCTTCATTTTGCTTTCAAAAGTGCTCAAAGTCGGCCCAGAAAATTACTGACTTCGGTTGATAAACAAAATGTCCT
TGCAACTTCTAAATTATGGCGAAAAATGGCTGATGAAATTGCTGACGAATATCCTGATGTACGATTAGAGCACCAATTGG
TCGATTCTTGTGCGATGTTACTGATTACTAATCCGCAACAATTTGATGTGATAGTCACTGAAAATCTATTTGGTGATATT
CTCTCTGATGAAGCAAGTAGTTTGGCCGGTAGCTTAGGAGTGATGCCTTCGAGTTCGCATGGATTTAACGGTTTAGCACT
CTATGAGCCAATTCATGGTTCGGCACCAGACATTGCAGGAAAAGGAATTGCGAACCCTGTTTCGATGATTCTATCAATTG
CCATGATGCTAAGAGAATCTTTTGGGCAAGAAGATGGGGCTACGATGATTGAGAAAGCCGTAACCCAAACTTTTACTGAC
TGA

Upstream 100 bases:

>100_bases
CCATTGCTTACATGAACGCTAATGTTTTAGTTCAAAAAGAAAATTTACAAGGAAAGGTTGAACAAATTTCAGCTCATGAT
GGAATTTAAGGTGAAAAATA

Downstream 100 bases:

>100_bases
ATTTTGACTAAAGCTTTAGGTGGGACTGCAACAACTAAAGAAATGACAGAAGCAATCCTGAAAAATTGTCAGTAAAATGC
GATTGAATAGTGAGCATTTT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSKKIVTLAGDGIGPEIMSAGLSVLKAVSKKIDFEYELEAKDFGGIAIDKHGHPLPEETLQAVKNADAILLAAIGHPKYN
NAKVRPEQGLLALRKELGLYANVRPLKIYPALKKLSPIRNVENVDFLMIRELTGGIYFGQHELADDKARDVNDYSADEIR
RILHFAFKSAQSRPRKLLTSVDKQNVLATSKLWRKMADEIADEYPDVRLEHQLVDSCAMLLITNPQQFDVIVTENLFGDI
LSDEASSLAGSLGVMPSSSHGFNGLALYEPIHGSAPDIAGKGIANPVSMILSIAMMLRESFGQEDGATMIEKAVTQTFTD

Sequences:

>Translated_320_residues
MSKKIVTLAGDGIGPEIMSAGLSVLKAVSKKIDFEYELEAKDFGGIAIDKHGHPLPEETLQAVKNADAILLAAIGHPKYN
NAKVRPEQGLLALRKELGLYANVRPLKIYPALKKLSPIRNVENVDFLMIRELTGGIYFGQHELADDKARDVNDYSADEIR
RILHFAFKSAQSRPRKLLTSVDKQNVLATSKLWRKMADEIADEYPDVRLEHQLVDSCAMLLITNPQQFDVIVTENLFGDI
LSDEASSLAGSLGVMPSSSHGFNGLALYEPIHGSAPDIAGKGIANPVSMILSIAMMLRESFGQEDGATMIEKAVTQTFTD
>Mature_319_residues
SKKIVTLAGDGIGPEIMSAGLSVLKAVSKKIDFEYELEAKDFGGIAIDKHGHPLPEETLQAVKNADAILLAAIGHPKYNN
AKVRPEQGLLALRKELGLYANVRPLKIYPALKKLSPIRNVENVDFLMIRELTGGIYFGQHELADDKARDVNDYSADEIRR
ILHFAFKSAQSRPRKLLTSVDKQNVLATSKLWRKMADEIADEYPDVRLEHQLVDSCAMLLITNPQQFDVIVTENLFGDIL
SDEASSLAGSLGVMPSSSHGFNGLALYEPIHGSAPDIAGKGIANPVSMILSIAMMLRESFGQEDGATMIEKAVTQTFTD

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=326, Percent_Identity=34.6625766871166, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI28178821, Length=296, Percent_Identity=30.4054054054054, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI28178816, Length=296, Percent_Identity=30.4054054054054, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI4758582, Length=320, Percent_Identity=29.375, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI28178838, Length=320, Percent_Identity=29.375, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI28178819, Length=141, Percent_Identity=35.4609929078014, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87081683, Length=333, Percent_Identity=47.1471471471471, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1788101, Length=309, Percent_Identity=34.3042071197411, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1787381, Length=360, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=333, Percent_Identity=33.3333333333333, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=300, Percent_Identity=31.3333333333333, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=324, Percent_Identity=31.4814814814815, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=327, Percent_Identity=29.0519877675841, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=334, Percent_Identity=46.7065868263473, Blast_Score=274, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=326, Percent_Identity=38.6503067484663, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324709, Length=323, Percent_Identity=36.2229102167183, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=326, Percent_Identity=31.9018404907975, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24643270, Length=306, Percent_Identity=36.2745098039216, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24643268, Length=306, Percent_Identity=36.2745098039216, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24661184, Length=326, Percent_Identity=31.2883435582822, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI161078637, Length=321, Percent_Identity=29.595015576324, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078635, Length=321, Percent_Identity=29.595015576324, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078633, Length=321, Percent_Identity=29.595015576324, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24650122, Length=321, Percent_Identity=29.595015576324, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078639, Length=318, Percent_Identity=29.874213836478, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281362242, Length=320, Percent_Identity=29.375, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24648872, Length=320, Percent_Identity=29.375, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI20130355, Length=324, Percent_Identity=25, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 35031; Mature: 34899

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKIVTLAGDGIGPEIMSAGLSVLKAVSKKIDFEYELEAKDFGGIAIDKHGHPLPEETL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCHHHH
QAVKNADAILLAAIGHPKYNNAKVRPEQGLLALRKELGLYANVRPLKIYPALKKLSPIRN
HHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCC
VENVDFLMIRELTGGIYFGQHELADDKARDVNDYSADEIRRILHFAFKSAQSRPRKLLTS
CCCCHHEEEEHHHCCEEECCHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VDKQNVLATSKLWRKMADEIADEYPDVRLEHQLVDSCAMLLITNPQQFDVIVTENLFGDI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHH
LSDEASSLAGSLGVMPSSSHGFNGLALYEPIHGSAPDIAGKGIANPVSMILSIAMMLRES
HHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FGQEDGATMIEKAVTQTFTD
HCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKKIVTLAGDGIGPEIMSAGLSVLKAVSKKIDFEYELEAKDFGGIAIDKHGHPLPEETL
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCHHHH
QAVKNADAILLAAIGHPKYNNAKVRPEQGLLALRKELGLYANVRPLKIYPALKKLSPIRN
HHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCC
VENVDFLMIRELTGGIYFGQHELADDKARDVNDYSADEIRRILHFAFKSAQSRPRKLLTS
CCCCHHEEEEHHHCCEEECCHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VDKQNVLATSKLWRKMADEIADEYPDVRLEHQLVDSCAMLLITNPQQFDVIVTENLFGDI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHH
LSDEASSLAGSLGVMPSSSHGFNGLALYEPIHGSAPDIAGKGIANPVSMILSIAMMLRES
HHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FGQEDGATMIEKAVTQTFTD
HCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400210; 11337471 [H]