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The map label for this gene is hisI

Identifier: 15673197

GI number: 15673197

Start: 1236981

End: 1237619

Strand: Direct

Name: hisI

Synonym: L0072

Alternate gene names: 15673197

Gene position: 1236981-1237619 (Clockwise)

Preceding gene: 15673196

Following gene: 15673198

Centisome position: 52.29

GC content: 33.65

Gene sequence:

>639_bases
ATGAGACCAGATTTTCATAAGCAGGAACTTATTCCTGTTATTGTTCAAGATTATCAAACAAATCAAGTATTAATGCTTGC
CTATACAAATGAAGTGGCTTTTGAGAAAATGCTTGAAACTGGTGAAACTTGGTTTTGGTCACGTTCACGACAAAAACTTT
GGCACAAAGGAGAAGAATCAGGTCATTTTCAAAAAATAAAAGGAATGCGTTTGGATTGCGACCAAGATACTTTATTGGTT
TTTGTTGAACAAATTGGTAATGCTTGCCATACCGGAACTTACTCTTGTTTTTATGATGAACTTATTCCTTTTGATGACTC
TGATATTTTTAGTGAGTTGGAGAAGCAAATCATCGACCGAAAGTTACATCCAGTCGAAAAATCTTATACGAATTATTTGC
TTGGAGAGGGCATTGATAAAGTATTAAAAAAAGTTGGTGAAGAAGCAAGTGAAGTGATTATTGCTAGTAAAAATTCAGAT
AAAGGAGAACTTTTAGGAGAAATTGATGATTTACTTTATCATTTGTTTGTATTAATGAATCAGCAAGGTATTTCTCTAGA
AGAAGTACGGCAAAAAGCAAAAGAACGGCATCAGCTAGAAGGAAATAAGAAGGAATTTCATACAAGGACGGCAGATTAA

Upstream 100 bases:

>100_bases
TTGCTTCATTATTTCATTATGGCGAAGCAACAGTTGATGAAGTTAAAGACGAACTTATAAAAAATAATATTCCAGCAAGA
ATTATTAAGAAGGAGACATT

Downstream 100 bases:

>100_bases
ATGAAAAAATTAGATTATCATTTCCACTCTCATTTTTCGGCTGATAGCGAGGAGCTTCCTAGAAAGCATGTGACAGAAGC
TATAGCACATGGTTTAGAAG

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRPDFHKQELIPVIVQDYQTNQVLMLAYTNEVAFEKMLETGETWFWSRSRQKLWHKGEESGHFQKIKGMRLDCDQDTLLV
FVEQIGNACHTGTYSCFYDELIPFDDSDIFSELEKQIIDRKLHPVEKSYTNYLLGEGIDKVLKKVGEEASEVIIASKNSD
KGELLGEIDDLLYHLFVLMNQQGISLEEVRQKAKERHQLEGNKKEFHTRTAD

Sequences:

>Translated_212_residues
MRPDFHKQELIPVIVQDYQTNQVLMLAYTNEVAFEKMLETGETWFWSRSRQKLWHKGEESGHFQKIKGMRLDCDQDTLLV
FVEQIGNACHTGTYSCFYDELIPFDDSDIFSELEKQIIDRKLHPVEKSYTNYLLGEGIDKVLKKVGEEASEVIIASKNSD
KGELLGEIDDLLYHLFVLMNQQGISLEEVRQKAKERHQLEGNKKEFHTRTAD
>Mature_212_residues
MRPDFHKQELIPVIVQDYQTNQVLMLAYTNEVAFEKMLETGETWFWSRSRQKLWHKGEESGHFQKIKGMRLDCDQDTLLV
FVEQIGNACHTGTYSCFYDELIPFDDSDIFSELEKQIIDRKLHPVEKSYTNYLLGEGIDKVLKKVGEEASEVIIASKNSD
KGELLGEIDDLLYHLFVLMNQQGISLEEVRQKAKERHQLEGNKKEFHTRTAD

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=189, Percent_Identity=43.3862433862434, Blast_Score=158, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319819, Length=200, Percent_Identity=33, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS2_LACLA (Q02130)

Other databases:

- EMBL:   U92974
- EMBL:   AE005176
- PIR:   C47754
- PIR:   G86776
- RefSeq:   NP_267371.1
- ProteinModelPortal:   Q02130
- GeneID:   1114863
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0072
- NMPDR:   fig|272623.1.peg.1250
- HOGENOM:   HBG294308
- OMA:   VMACKDD
- ProtClustDB:   PRK02759
- BioCyc:   LLAC272623:L0072-MONOMER
- BRENDA:   3.5.4.19
- BRENDA:   3.6.1.31
- GO:   GO:0005737
- HAMAP:   MF_01019
- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610
- TIGRFAMs:   TIGR03188

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH

EC number: =3.5.4.19; =3.6.1.31

Molecular weight: Translated: 24717; Mature: 24717

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPDFHKQELIPVIVQDYQTNQVLMLAYTNEVAFEKMLETGETWFWSRSRQKLWHKGEES
CCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
GHFQKIKGMRLDCDQDTLLVFVEQIGNACHTGTYSCFYDELIPFDDSDIFSELEKQIIDR
CCHHHHCCCCCCCCCHHHHHHHHHHCCHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
KLHPVEKSYTNYLLGEGIDKVLKKVGEEASEVIIASKNSDKGELLGEIDDLLYHLFVLMN
HCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHC
QQGISLEEVRQKAKERHQLEGNKKEFHTRTAD
CCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCC
>Mature Secondary Structure
MRPDFHKQELIPVIVQDYQTNQVLMLAYTNEVAFEKMLETGETWFWSRSRQKLWHKGEES
CCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
GHFQKIKGMRLDCDQDTLLVFVEQIGNACHTGTYSCFYDELIPFDDSDIFSELEKQIIDR
CCHHHHCCCCCCCCCHHHHHHHHHHCCHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
KLHPVEKSYTNYLLGEGIDKVLKKVGEEASEVIIASKNSDKGELLGEIDDLLYHLFVLMN
HCCCHHHHHHHHHHHCCHHHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHC
QQGISLEEVRQKAKERHQLEGNKKEFHTRTAD
CCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400209; 11337471