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The map label for this gene is hisF

Identifier: 15673196

GI number: 15673196

Start: 1236205

End: 1236984

Strand: Direct

Name: hisF

Synonym: L0071

Alternate gene names: 15673196

Gene position: 1236205-1236984 (Clockwise)

Preceding gene: 15673195

Following gene: 15673197

Centisome position: 52.26

GC content: 37.05

Gene sequence:

>780_bases
ATGCTAACTAAAAGAATCATCCCTTGTCTTGATATTAAAAATGGTAAGGTTGTTAAAGGAATTAATTTTGTGAGTTTAAG
AGAAATAGGTGATTCCGTGGAATTGGCCAAAATATATGAAGAACAGTGTGCAGATGAAATTGTTTTTCTTGATATTACAG
CATCTTTTGAAGAACGTGAAATTATTGGTGAATTAATTGGTCGGGCGGCGCGTGAACTTTCAATTCCATTAACCTTTGGT
GGAGGAATTCGTTCAATTGACGATTTTAGAAGAATTCTTGCTAGAGGAGCTGATAAAGTATCCGTCAACTCAGCTGCAAT
TGAAAATCCTGAACTTATTCGTCAAGCGGCTAACGAACTTGGCGTTCAATGTGTTGTTGTTGCTATTGATGCTAAAAAAC
GAGCAGACCATAGGGGATATGATGTCTATATCAAAGGTGGGCGTGAAAATGCAGGTCTTGACTTAGTTGATTGGGCTAAA
AAGTGTGAAAGATTAGGAGCAGGCGAAATTTTACTAACCTCAATGGATAAGGATGGAACAAAGACAGGCTACGATTTGGA
AATGCTGAATGACGTTTGTACTGCTGTTAATATTCCTGTTGTAGCAAGTGGCGGTTGTGGGAAGATTTCGGACATTGTTG
AAGTTTTTCAAAATACAAGAAGCGATGCTGCCCTTGTTGCTTCATTATTTCATTATGGCGAAGCAACAGTTGATGAAGTT
AAAGACGAACTTATAAAAAATAATATTCCAGCAAGAATTATTAAGAAGGAGACATTATGA

Upstream 100 bases:

>100_bases
AATTCTGATATTCAACGTGCAACTCGTTCTGACTTCTATGGAATTATCGTTGGGAAAGCTTACTATGAGAAGAAAATTAA
TCTTGAAAAGGAGTTCAGGA

Downstream 100 bases:

>100_bases
GACCAGATTTTCATAAGCAGGAACTTATTCCTGTTATTGTTCAAGATTATCAAACAAATCAAGTATTAATGCTTGCCTAT
ACAAATGAAGTGGCTTTTGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLTKRIIPCLDIKNGKVVKGINFVSLREIGDSVELAKIYEEQCADEIVFLDITASFEEREIIGELIGRAARELSIPLTFG
GGIRSIDDFRRILARGADKVSVNSAAIENPELIRQAANELGVQCVVVAIDAKKRADHRGYDVYIKGGRENAGLDLVDWAK
KCERLGAGEILLTSMDKDGTKTGYDLEMLNDVCTAVNIPVVASGGCGKISDIVEVFQNTRSDAALVASLFHYGEATVDEV
KDELIKNNIPARIIKKETL

Sequences:

>Translated_259_residues
MLTKRIIPCLDIKNGKVVKGINFVSLREIGDSVELAKIYEEQCADEIVFLDITASFEEREIIGELIGRAARELSIPLTFG
GGIRSIDDFRRILARGADKVSVNSAAIENPELIRQAANELGVQCVVVAIDAKKRADHRGYDVYIKGGRENAGLDLVDWAK
KCERLGAGEILLTSMDKDGTKTGYDLEMLNDVCTAVNIPVVASGGCGKISDIVEVFQNTRSDAALVASLFHYGEATVDEV
KDELIKNNIPARIIKKETL
>Mature_259_residues
MLTKRIIPCLDIKNGKVVKGINFVSLREIGDSVELAKIYEEQCADEIVFLDITASFEEREIIGELIGRAARELSIPLTFG
GGIRSIDDFRRILARGADKVSVNSAAIENPELIRQAANELGVQCVVVAIDAKKRADHRGYDVYIKGGRENAGLDLVDWAK
KCERLGAGEILLTSMDKDGTKTGYDLEMLNDVCTAVNIPVVASGGCGKISDIVEVFQNTRSDAALVASLFHYGEATVDEV
KDELIKNNIPARIIKKETL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=42.8015564202335, Blast_Score=208, Evalue=2e-55,
Organism=Escherichia coli, GI87082028, Length=241, Percent_Identity=28.6307053941909, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=35.8730158730159, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28282; Mature: 28282

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDIKNGKVVKGINFVSLREIGDSVELAKIYEEQCADEIVFLDITASFEERE
CCCCCCCCCEECCCCCEEECCCEEEHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHH
IIGELIGRAARELSIPLTFGGGIRSIDDFRRILARGADKVSVNSAAIENPELIRQAANEL
HHHHHHHHHHHHEECEEEECCCCCCHHHHHHHHHCCCCCEECCHHHCCCHHHHHHHHHHC
GVQCVVVAIDAKKRADHRGYDVYIKGGRENAGLDLVDWAKKCERLGAGEILLTSMDKDGT
CCEEEEEEEECHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
KTGYDLEMLNDVCTAVNIPVVASGGCGKISDIVEVFQNTRSDAALVASLFHYGEATVDEV
CCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHH
KDELIKNNIPARIIKKETL
HHHHHHCCCCHHHHCCCCC
>Mature Secondary Structure
MLTKRIIPCLDIKNGKVVKGINFVSLREIGDSVELAKIYEEQCADEIVFLDITASFEERE
CCCCCCCCCEECCCCCEEECCCEEEHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHH
IIGELIGRAARELSIPLTFGGGIRSIDDFRRILARGADKVSVNSAAIENPELIRQAANEL
HHHHHHHHHHHHEECEEEECCCCCCHHHHHHHHHCCCCCEECCHHHCCCHHHHHHHHHHC
GVQCVVVAIDAKKRADHRGYDVYIKGGRENAGLDLVDWAKKCERLGAGEILLTSMDKDGT
CCEEEEEEEECHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
KTGYDLEMLNDVCTAVNIPVVASGGCGKISDIVEVFQNTRSDAALVASLFHYGEATVDEV
CCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHH
KDELIKNNIPARIIKKETL
HHHHHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400209; 11337471 [H]