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The map label for this gene is potD

Identifier: 15673148

GI number: 15673148

Start: 1178602

End: 1179669

Strand: Direct

Name: potD

Synonym: L178329

Alternate gene names: 15673148

Gene position: 1178602-1179669 (Clockwise)

Preceding gene: 15673147

Following gene: 15673149

Centisome position: 49.82

GC content: 34.27

Gene sequence:

>1068_bases
TTGAAAAAATTATCATACTTCTTTGTAGGAATTTTGATTATTACTGCCTCGTTAGGACTGATTACTAATAGAATGAAAGC
TGCTGATGGAGTCAAAACTCAAAATAATAGCCTGACCATTTTTAACTGGGGAGAGTATATCGACCCAGACTTGATTAGTA
AATTTCAAAAAGAAACAGGTTATAAAGTAAACTATATGACCTTTGATTCCAATGAAGCCATGTATTCAAAAGTCAAACAA
GGAGGAACAGCTTATGACCTGATTGTTCCTTCCGATTACATGATTGAAAAAATGGCCAAAGAAAATTTGCTCATTAAGCT
AGACCATAGTAAGATTAAAGGTTTAAAAGATGATGACCCTAAACTCCTAAATCCGGCTTTTGACCAAGGAAATCAATATT
CTGTTCCATACTTTTGGGGAACATTAGGGATTGTTTATAATTCAAAAACAGTAACAAGGCCACCAAAAACGTGGAATGAT
ATCTGGTCACCAACTTACAAAAACTCAATTTTACTGACAGACAGTGTGCGTGACGTTATGGCAATGGTCCTTAGTAAACA
TGGATATTCATTAAATACAACCAACCAAAAAGAGCTAGATATAGCCTATCAAGATCTTCTAAAACTTACGCCAAATGTAA
AAGCAATTTTGGGTGATGAAATCATGAATTACATGATTAATAATGAAACACCTTTAGCTGTCGTCTATAGTGGACAAGTA
GCAGAGATGACCTCTGAAAATAAAAATTTGAAATACGTTATCCCCGAGCGAACAAACATTTGGTATGACAATTTAGCCAT
TCCAAAAACAGCTAAAAACGTCAAAGCAGCTTACGCATTTATCAATTTCATGCAAGAACCCAAAAATGCAGCCCAAAATG
CGCAGTGGATTGGTTATGCAACCCCAAATTTGAAAGCCAAAGCCCTCTTACCCAAAGAAATTCGAGATAATAAATCCTTT
TATCCAGATGAAGCAACCATCAAAGACGATGAAGCCTACAAAAACCTTAATCCATATTGGACTGGAATTTATAATGATTT
ATATTTAGAATTTAAGATGAATAAATAA

Upstream 100 bases:

>100_bases
ATTATATTATGAGAGATTCAGAAACTGTTGGAAAATCTTCAAATCTGACTTAAAACTATTTTACTGATACTTTGTAGCTC
AAAGTATAGAAAGGAGAAAC

Downstream 100 bases:

>100_bases
AAAGTGATATTTAGAGTGATTTAGTCTAATTTGAAGAAAAATGCTTGGACGTAAATAAACAACGAGTATATATAAATAAG
AAATAATTTAAATTTGAATG

Product: spermidine/putrescine ABC transporter substrate binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKKLSYFFVGILIITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETGYKVNYMTFDSNEAMYSKVKQ
GGTAYDLIVPSDYMIEKMAKENLLIKLDHSKIKGLKDDDPKLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVTRPPKTWND
IWSPTYKNSILLTDSVRDVMAMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQV
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAQWIGYATPNLKAKALLPKEIRDNKSF
YPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK

Sequences:

>Translated_355_residues
MKKLSYFFVGILIITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETGYKVNYMTFDSNEAMYSKVKQ
GGTAYDLIVPSDYMIEKMAKENLLIKLDHSKIKGLKDDDPKLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVTRPPKTWND
IWSPTYKNSILLTDSVRDVMAMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQV
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAQWIGYATPNLKAKALLPKEIRDNKSF
YPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK
>Mature_355_residues
MKKLSYFFVGILIITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETGYKVNYMTFDSNEAMYSKVKQ
GGTAYDLIVPSDYMIEKMAKENLLIKLDHSKIKGLKDDDPKLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVTRPPKTWND
IWSPTYKNSILLTDSVRDVMAMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQV
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAQWIGYATPNLKAKALLPKEIRDNKSF
YPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=324, Percent_Identity=42.9012345679012, Blast_Score=250, Evalue=9e-68,
Organism=Escherichia coli, GI1787078, Length=327, Percent_Identity=31.8042813455657, Blast_Score=161, Evalue=6e-41,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40616; Mature: 40616

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSYFFVGILIITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHHHHHHHHHCC
YKVNYMTFDSNEAMYSKVKQGGTAYDLIVPSDYMIEKMAKENLLIKLDHSKIKGLKDDDP
CEEEEEEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCEEEEECHHHHCCCCCCCC
KLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVTRPPKTWNDIWSPTYKNSILLTDSVRDVM
CCCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHHH
AMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQV
HHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCE
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAQWIGYA
EEHHCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TPNLKAKALLPKEIRDNKSFYPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK
CCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEEEEEEEECC
>Mature Secondary Structure
MKKLSYFFVGILIITASLGLITNRMKAADGVKTQNNSLTIFNWGEYIDPDLISKFQKETG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCHHHHHHHHHHCC
YKVNYMTFDSNEAMYSKVKQGGTAYDLIVPSDYMIEKMAKENLLIKLDHSKIKGLKDDDP
CEEEEEEECCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCEEEEECHHHHCCCCCCCC
KLLNPAFDQGNQYSVPYFWGTLGIVYNSKTVTRPPKTWNDIWSPTYKNSILLTDSVRDVM
CCCCCCCCCCCEEECCEEEEEEEEEECCCCCCCCCCCHHHHCCCCCCCCEEEEHHHHHHH
AMVLSKHGYSLNTTNQKELDIAYQDLLKLTPNVKAILGDEIMNYMINNETPLAVVYSGQV
HHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCE
AEMTSENKNLKYVIPERTNIWYDNLAIPKTAKNVKAAYAFINFMQEPKNAAQNAQWIGYA
EEHHCCCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
TPNLKAKALLPKEIRDNKSFYPDEATIKDDEAYKNLNPYWTGIYNDLYLEFKMNK
CCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]