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The map label for this gene is menF

Identifier: 15672714

GI number: 15672714

Start: 738133

End: 739347

Strand: Reverse

Name: menF

Synonym: L0168

Alternate gene names: 15672714

Gene position: 739347-738133 (Counterclockwise)

Preceding gene: 15672718

Following gene: 15672713

Centisome position: 31.25

GC content: 35.23

Gene sequence:

>1215_bases
ATGAAGATTTGTAATTTTAGGGCTCATTATTCCCCTACTATGCTGAGCTATGTTATAATTTTTATGGTAGAAAAAATGAA
TTATATAAAAATCAATTTAAAATTAAATAACCCCTTAGCTTTCTGGGCAAACTTTTCAGAAAGTGAGCGTTTCTTTTGGT
TTGACCCTCAAAAAGAAACTTTTGTTATTGGCTGTGAGCGTTTGAAAGCCATTGACCTTGAACATGATAAGTTTTCTGAC
TATACCTATCTCTTTCATAGTCAGACTTTTTTTGATGACATTAAAGGAGAACTTTGGGAAAATTTCGGTGGCGAAACCAT
TGCTTTTAAACATTATTTCGTTGTCAATAAGGAAAATTCTTACACCCTCACTTGTGATAGCCCACGAGAAATTCCTGAAA
TCACTATTCCAAAATTTAAGCATCAACTCACTGAAAAAGCTTCTGATTTTTCGGCTTGGCAAGAACTTTTTTATGCCATT
CAAAAAAATTTTGCTGACGGAAAGAGTCGAAAAATTGTCGCTTCAAGAGAACTTGAATTTACAAGTCAAACCTCTTTTGA
ACTCGAAAGTATCATCCAAAATTTATTAGATAATAATCCAAATGCTTTTATTTTTGCTTATCAAAAAGGAAAAAGAATTT
TCCTTGGAGCATCGCCCGAAATTTTAGTTCAAAAAGAAAATGACCAACTCCTTAGTTATGCCTTAGCTGGAACTTTTCCA
AAAACAATGGAAAATGCGGGGCAAAAATTGCTGACAGACCCCAAAAATCTTTTAGAGCATGATATTGTTGTCCAAAAAAT
AAAAAGAAATCTGCTTGAAAAAGCCGAGACAGTAACGGTTGGAACAACCGAATTAATGGAGTTGAAAAATGTTTATCATC
TTCGGACAATTTTAAGTGCTAAAAATTCTGAGCTTTCACTTATTGATTGGACAAAACAGCTTCACCCAACGCCTGCTCTT
GGAGGAGAACCTCGTCATGAAGCCCTCGAATTCCTCAGAAAGCATGAAAATCATGAACGAGGACTATACGCTGCACCTCT
CGGATTAATTGACAGCAAAGGAAATGGCACAATGATTGTCGGGATTCGTTCGGCTTTGATTGTGGACAAGAAACTTTATG
CTTATGCAGGGTGTGGAATTATCCCTTCTTCCGATGCTTTAGAAGAATTTAGGGAAACCAAGGTAAAATTAAAAACCATT
TTAGAAGCTTTGTAA

Upstream 100 bases:

>100_bases
AGGTATCTAGGAAAGGCACCATAGTGGCTTCTGTCTGCGCGTTCACGCTTTAGCGTGTTATAAGCAGTGATACTTATATC
AGAAAGTTTATGAAAAATGA

Downstream 100 bases:

>100_bases
CTTTTTAAAATATTTTACGATTTTAGATTTTTAAATTAGAAATGGAGATAGTCATGACCAATGAATATTTAGCTCCCTTT
GTTGATGAACTTTTCAATTT

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKICNFRAHYSPTMLSYVIIFMVEKMNYIKINLKLNNPLAFWANFSESERFFWFDPQKETFVIGCERLKAIDLEHDKFSD
YTYLFHSQTFFDDIKGELWENFGGETIAFKHYFVVNKENSYTLTCDSPREIPEITIPKFKHQLTEKASDFSAWQELFYAI
QKNFADGKSRKIVASRELEFTSQTSFELESIIQNLLDNNPNAFIFAYQKGKRIFLGASPEILVQKENDQLLSYALAGTFP
KTMENAGQKLLTDPKNLLEHDIVVQKIKRNLLEKAETVTVGTTELMELKNVYHLRTILSAKNSELSLIDWTKQLHPTPAL
GGEPRHEALEFLRKHENHERGLYAAPLGLIDSKGNGTMIVGIRSALIVDKKLYAYAGCGIIPSSDALEEFRETKVKLKTI
LEAL

Sequences:

>Translated_404_residues
MKICNFRAHYSPTMLSYVIIFMVEKMNYIKINLKLNNPLAFWANFSESERFFWFDPQKETFVIGCERLKAIDLEHDKFSD
YTYLFHSQTFFDDIKGELWENFGGETIAFKHYFVVNKENSYTLTCDSPREIPEITIPKFKHQLTEKASDFSAWQELFYAI
QKNFADGKSRKIVASRELEFTSQTSFELESIIQNLLDNNPNAFIFAYQKGKRIFLGASPEILVQKENDQLLSYALAGTFP
KTMENAGQKLLTDPKNLLEHDIVVQKIKRNLLEKAETVTVGTTELMELKNVYHLRTILSAKNSELSLIDWTKQLHPTPAL
GGEPRHEALEFLRKHENHERGLYAAPLGLIDSKGNGTMIVGIRSALIVDKKLYAYAGCGIIPSSDALEEFRETKVKLKTI
LEAL
>Mature_404_residues
MKICNFRAHYSPTMLSYVIIFMVEKMNYIKINLKLNNPLAFWANFSESERFFWFDPQKETFVIGCERLKAIDLEHDKFSD
YTYLFHSQTFFDDIKGELWENFGGETIAFKHYFVVNKENSYTLTCDSPREIPEITIPKFKHQLTEKASDFSAWQELFYAI
QKNFADGKSRKIVASRELEFTSQTSFELESIIQNLLDNNPNAFIFAYQKGKRIFLGASPEILVQKENDQLLSYALAGTFP
KTMENAGQKLLTDPKNLLEHDIVVQKIKRNLLEKAETVTVGTTELMELKNVYHLRTILSAKNSELSLIDWTKQLHPTPAL
GGEPRHEALEFLRKHENHERGLYAAPLGLIDSKGNGTMIVGIRSALIVDKKLYAYAGCGIIPSSDALEEFRETKVKLKTI
LEAL

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=239, Percent_Identity=33.4728033472803, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI87082077, Length=337, Percent_Identity=29.080118694362, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787518, Length=294, Percent_Identity=22.7891156462585, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788114, Length=260, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=298, Percent_Identity=24.1610738255034, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 46451; Mature: 46451

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKICNFRAHYSPTMLSYVIIFMVEKMNYIKINLKLNNPLAFWANFSESERFFWFDPQKET
CCEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCCEEEEECCCCCE
FVIGCERLKAIDLEHDKFSDYTYLFHSQTFFDDIKGELWENFGGETIAFKHYFVVNKENS
EEEEEHHHEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
YTLTCDSPREIPEITIPKFKHQLTEKASDFSAWQELFYAIQKNFADGKSRKIVASRELEF
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
TSQTSFELESIIQNLLDNNPNAFIFAYQKGKRIFLGASPEILVQKENDQLLSYALAGTFP
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCEEEEECCCCHHHHHHHHCCCH
KTMENAGQKLLTDPKNLLEHDIVVQKIKRNLLEKAETVTVGTTELMELKNVYHLRTILSA
HHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHHHHC
KNSELSLIDWTKQLHPTPALGGEPRHEALEFLRKHENHERGLYAAPLGLIDSKGNGTMIV
CCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEECCCCCEEEE
GIRSALIVDKKLYAYAGCGIIPSSDALEEFRETKVKLKTILEAL
EEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKICNFRAHYSPTMLSYVIIFMVEKMNYIKINLKLNNPLAFWANFSESERFFWFDPQKET
CCEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCCEEEEECCCCCE
FVIGCERLKAIDLEHDKFSDYTYLFHSQTFFDDIKGELWENFGGETIAFKHYFVVNKENS
EEEEEHHHEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCC
YTLTCDSPREIPEITIPKFKHQLTEKASDFSAWQELFYAIQKNFADGKSRKIVASRELEF
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
TSQTSFELESIIQNLLDNNPNAFIFAYQKGKRIFLGASPEILVQKENDQLLSYALAGTFP
CCCCHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCEEEEECCCCHHHHHHHHCCCH
KTMENAGQKLLTDPKNLLEHDIVVQKIKRNLLEKAETVTVGTTELMELKNVYHLRTILSA
HHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHHHHHHC
KNSELSLIDWTKQLHPTPALGGEPRHEALEFLRKHENHERGLYAAPLGLIDSKGNGTMIV
CCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEECCCCCEEEE
GIRSALIVDKKLYAYAGCGIIPSSDALEEFRETKVKLKTILEAL
EEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]