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The map label for this gene is ansB

Identifier: 15672718

GI number: 15672718

Start: 742698

End: 743669

Strand: Reverse

Name: ansB

Synonym: L142722

Alternate gene names: 15672718

Gene position: 743669-742698 (Counterclockwise)

Preceding gene: 15672722

Following gene: 15672714

Centisome position: 31.44

GC content: 37.76

Gene sequence:

>972_bases
ATGATGAAAAAAATCTTAATTCTCCATACTGGTGGAACAATTTCGATGGCGGAAGATGAAAATGGTCATGTTTCCCCAAA
TGCAGTCAATCCAATGAATGCTGTGAGCATGCAGTTTGATGAAGTAAACTTAGTGACAGAGGATATTTTTAACTTACCTA
GTCCACATGTCACTTTATCAGATATGTTGGTCTTGAAAAATCGAATCAAAACAGCTTTTATTGCTGAGGATTTTGATGGA
GTGGTCATTACACATGGAACTGACACACTTGAAGAAACAGCTTTTTTCCTTGACACTACTCTTTCGCGTGGTAAACCAAT
TGTTTTAACTGGTGCCATGCGCTCAAGTAATGAATTAGGAACTGACGGAATTTATAATTTACTGACAGCTATTCGCGTCG
CTGCTGACAGTAATTCAAGTGACCGTGGCGTACTTGTTGTTATGAATGACGAGATTCATTCTGCTCGTTATGTCACTAAA
ACACACACGACTAATGTTTCAACTTTTCAAACACCTACACATGGTCCTGTCGGTTTACTGACAAAAAATAAAATTATTTA
TTTCCATAAAGATAGTGAAAATCAACATTTAGATGTTGCAACTGTTCATGGAAAAGTGCCAATTGTCAAAGCATTTGCTG
GGATGACTGGGGAAATTTTAGATTTACTCAATTTGGAACAACTAGACGGTCTTGTCATTGAAGCATTAGGTGCGGGAAAT
CTCCCACCTATTGCTAGTCAATCGCTTCAAAGATTACTAGATGCTAAGGTTCCTGTCGTTCTTGTTTCTCGTTGTTTTAA
CGGAATTGCTGAACCTGTCTATTCCTATCAAGGCGGTGGTTCACAACTTTATGAAGCAGGCGTGATGTTTGCTGATGAAG
TCAATTCTCAAAAAGCGCGTATTAAATTGATTATTGCATTGAATGCTGGCTTGAAAGATTTATCAGAATTACGACCTTTC
TTGGAGAATTAA

Upstream 100 bases:

>100_bases
TTCTGGACTAAATTTTGATATAATGCTAATGCTGGCTTTTATTCGGAGTCTTTAAATTTAAATATTTAATTATTGTTTAT
TATAGATAGATAAGGAAATA

Downstream 100 bases:

>100_bases
AAAAAATTACTGACAAAAAAGAATCATCAGCTGACTGATGATTCTTTCTTTTACTGATTTAAAGCTCATTAATCAATCGC
TACTGACAGAATTATCAGCT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MMKKILILHTGGTISMAEDENGHVSPNAVNPMNAVSMQFDEVNLVTEDIFNLPSPHVTLSDMLVLKNRIKTAFIAEDFDG
VVITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELGTDGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTK
THTTNVSTFQTPTHGPVGLLTKNKIIYFHKDSENQHLDVATVHGKVPIVKAFAGMTGEILDLLNLEQLDGLVIEALGAGN
LPPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKARIKLIIALNAGLKDLSELRPF
LEN

Sequences:

>Translated_323_residues
MMKKILILHTGGTISMAEDENGHVSPNAVNPMNAVSMQFDEVNLVTEDIFNLPSPHVTLSDMLVLKNRIKTAFIAEDFDG
VVITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELGTDGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTK
THTTNVSTFQTPTHGPVGLLTKNKIIYFHKDSENQHLDVATVHGKVPIVKAFAGMTGEILDLLNLEQLDGLVIEALGAGN
LPPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKARIKLIIALNAGLKDLSELRPF
LEN
>Mature_323_residues
MMKKILILHTGGTISMAEDENGHVSPNAVNPMNAVSMQFDEVNLVTEDIFNLPSPHVTLSDMLVLKNRIKTAFIAEDFDG
VVITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELGTDGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTK
THTTNVSTFQTPTHGPVGLLTKNKIIYFHKDSENQHLDVATVHGKVPIVKAFAGMTGEILDLLNLEQLDGLVIEALGAGN
LPPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKARIKLIIALNAGLKDLSELRPF
LEN

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=299, Percent_Identity=23.7458193979933, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789327, Length=284, Percent_Identity=36.9718309859155, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1788065, Length=321, Percent_Identity=26.791277258567, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193207372, Length=359, Percent_Identity=25.0696378830084, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558280, Length=359, Percent_Identity=25.0696378830084, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI193207374, Length=359, Percent_Identity=25.0696378830084, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=359, Percent_Identity=25.0696378830084, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320527, Length=331, Percent_Identity=32.3262839879154, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323189, Length=324, Percent_Identity=29.320987654321, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6323187, Length=324, Percent_Identity=29.320987654321, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6323186, Length=324, Percent_Identity=29.320987654321, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6323184, Length=324, Percent_Identity=29.320987654321, Blast_Score=125, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24645530, Length=344, Percent_Identity=24.7093023255814, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35012; Mature: 35012

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKILILHTGGTISMAEDENGHVSPNAVNPMNAVSMQFDEVNLVTEDIFNLPSPHVTLS
CCCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHEEEECHHHHHHHHHHCCCCCCEEHH
DMLVLKNRIKTAFIAEDFDGVVITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELG
HHHHHHHHHHEEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
TDGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTKTHTTNVSTFQTPTHGPVGLL
CHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCCEEEE
TKNKIIYFHKDSENQHLDVATVHGKVPIVKAFAGMTGEILDLLNLEQLDGLVIEALGAGN
ECCEEEEEEECCCCCEEEEEEECCCCCHHHHHCCCCHHHHHHHCHHHHCCEEEEECCCCC
LPPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKAR
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEECCCCCCCEE
IKLIIALNAGLKDLSELRPFLEN
EEEEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure
MMKKILILHTGGTISMAEDENGHVSPNAVNPMNAVSMQFDEVNLVTEDIFNLPSPHVTLS
CCCEEEEEECCCEEEEECCCCCCCCCCCCCCCHHHEEEECHHHHHHHHHHCCCCCCEEHH
DMLVLKNRIKTAFIAEDFDGVVITHGTDTLEETAFFLDTTLSRGKPIVLTGAMRSSNELG
HHHHHHHHHHEEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
TDGIYNLLTAIRVAADSNSSDRGVLVVMNDEIHSARYVTKTHTTNVSTFQTPTHGPVGLL
CHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEECCCCCCCEEEE
TKNKIIYFHKDSENQHLDVATVHGKVPIVKAFAGMTGEILDLLNLEQLDGLVIEALGAGN
ECCEEEEEEECCCCCEEEEEEECCCCCHHHHHCCCCHHHHHHHCHHHHCCEEEEECCCCC
LPPIASQSLQRLLDAKVPVVLVSRCFNGIAEPVYSYQGGGSQLYEAGVMFADEVNSQKAR
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEECCCCCCCEE
IKLIIALNAGLKDLSELRPFLEN
EEEEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]