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The map label for this gene is menD

Identifier: 15672713

GI number: 15672713

Start: 736397

End: 738079

Strand: Reverse

Name: menD

Synonym: L0169

Alternate gene names: 15672713

Gene position: 738079-736397 (Counterclockwise)

Preceding gene: 15672714

Following gene: 15672712

Centisome position: 31.2

GC content: 36.66

Gene sequence:

>1683_bases
ATGACCAATGAATATTTAGCTCCCTTTGTTGATGAACTTTTCAATTTAGGGGTACGTGAAGCTGTTTTTAGTCCAGGTTC
ACGTTCAACGGCTTTAGCCATGCTCTTTGAGGAATACAAAAAATACGATACTTATGTCAACATTGATGAACGTTCGGCTG
CTTTTTTTGCTCTGGGAATTGCTAAAGCGAAAAAACGTCCTGTTGTTTTAGTTTGTACTTCAGGTTCTGCTGCGGCTCAT
CATTTTCCAGCTGTCCTTGAAGCAAAAATGAGTCGAGTTCCCCTTATTATTTTAACGGCGGACAGACCGGCTGAGCTTCA
ATTCGTTGGAGCTCCTCAAACAGTCGACCAGACCAGATTTTTTGGAAATTTTGTTAATCATTTTGAAAATCTTGAAGCTC
CACATATTCAAAAGCAGTCTCAAACAGAAAACTTTTGGACCTATCCACGTAAAGTCGCACAACGAGCTGTCTTATCAGCT
ATTTCCCCTCTTTCGGGTCCTGTGCAAATCAATGTTCCCTTGCGTGATCTTTTAGTTCCAGAATTAAAAAGTGAAAATTA
TGAAAAAGGACGTTCTAAACATGCCTTTAAATTCTATGAGGGACAAGCACAAGTAATACTTCCATTTGATGAAGCATTGC
TTTCTGGAAAAACATTAATTTTAGCCGGTGCCAATTTTGACAAAGATTATTCTGAAGCACTTTTAAAACTTGCGGAACAA
TTAAAAGCTCCAATTTTAGCGGATCCACTTTCTAATCTGCGTAATCATAATCATCCTTTGGTCATAGACTCTTATGATGC
TTTTTTAGCAAATAATGATTTAAAAACTCAATTAAAAGCGGATGCTATTCTGCTTTTTGGACAAATGCCTGTTTCTAAAC
GTTTGCAACAATTCGTTGCTTTCAATAACGAAGCTGAATTTATTCAAGTTGACCCTGCTCTTGATTATCGCAACCCTAGT
TTGACAACGACAACGATGGTTCAGTCTGATGTTCTCCCTTTTGCTTCTTCAATTAAAAAAGTAAATTCTGATAGTTCTTA
TTTGGAAAAATGGCAAAATGCTCAAGAAAAAATGCGACAACTTCTTGAAAAAGTGACTTATGAAGAAAGTCCTTTTGAAG
GACGTTATGTCCAAGAACTACAAAAACATTTGGAAGCGCTGGATGCTCAACTTCTTGTTTCAAATAGTATGGAAATCAGA
GATATTGATTATTGGTGGAAAAAAGCGGATGCCAAAGTTAGAATTTTAGGAAATCGTGGTGTGAATGGCATTGACGGGAC
TGAATCAACTGCACTCGGAATTGCTACAACGGGTAAACCGACGGTTTTATTGACTGGGGATTTGTCAATGCTACACGATT
TAAATGGTTTAATTGTTGGTAAAACACATGAACTTAATCTGACTATTGTGCTATTTAATAATGACGGTGGTGGGATTTTT
CATCATTTAGCTCAAAAAGGCGTACCGAATTTTGATTATCTTTTTTCAACTCCACATGGTTTAAATTTTGCTGGACTGGC
TGAGTTGACTGGTTTAGATTATCATTTGGTCAGTGGCTATGCTGATTTTGGTCAGCAATTTGAAGCTTCTCTTCATCAAT
CTGGCATTCATCTTTTGGAAATTAAAACGGATAAAGATTTAAGTCTTGCTTTACATCAAAAATATACAACTTATGAAAAT
TGA

Upstream 100 bases:

>100_bases
TTAGGGAAACCAAGGTAAAATTAAAAACCATTTTAGAAGCTTTGTAACTTTTTAAAATATTTTACGATTTTAGATTTTTA
AATTAGAAATGGAGATAGTC

Downstream 100 bases:

>100_bases
TAAAAAAATAATTACTATTGATGGTTTTGATTATGCTGTCCGTATTTGGGGGACTGGAGAGGTCCTATTTGCACTGCATG
GTTTTTCTGAAAGTTCTAAT

Product: 2-oxoglutarate decarboxylase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGIAKAKKRPVVLVCTSGSAAAH
HFPAVLEAKMSRVPLIILTADRPAELQFVGAPQTVDQTRFFGNFVNHFENLEAPHIQKQSQTENFWTYPRKVAQRAVLSA
ISPLSGPVQINVPLRDLLVPELKSENYEKGRSKHAFKFYEGQAQVILPFDEALLSGKTLILAGANFDKDYSEALLKLAEQ
LKAPILADPLSNLRNHNHPLVIDSYDAFLANNDLKTQLKADAILLFGQMPVSKRLQQFVAFNNEAEFIQVDPALDYRNPS
LTTTTMVQSDVLPFASSIKKVNSDSSYLEKWQNAQEKMRQLLEKVTYEESPFEGRYVQELQKHLEALDAQLLVSNSMEIR
DIDYWWKKADAKVRILGNRGVNGIDGTESTALGIATTGKPTVLLTGDLSMLHDLNGLIVGKTHELNLTIVLFNNDGGGIF
HHLAQKGVPNFDYLFSTPHGLNFAGLAELTGLDYHLVSGYADFGQQFEASLHQSGIHLLEIKTDKDLSLALHQKYTTYEN

Sequences:

>Translated_560_residues
MTNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGIAKAKKRPVVLVCTSGSAAAH
HFPAVLEAKMSRVPLIILTADRPAELQFVGAPQTVDQTRFFGNFVNHFENLEAPHIQKQSQTENFWTYPRKVAQRAVLSA
ISPLSGPVQINVPLRDLLVPELKSENYEKGRSKHAFKFYEGQAQVILPFDEALLSGKTLILAGANFDKDYSEALLKLAEQ
LKAPILADPLSNLRNHNHPLVIDSYDAFLANNDLKTQLKADAILLFGQMPVSKRLQQFVAFNNEAEFIQVDPALDYRNPS
LTTTTMVQSDVLPFASSIKKVNSDSSYLEKWQNAQEKMRQLLEKVTYEESPFEGRYVQELQKHLEALDAQLLVSNSMEIR
DIDYWWKKADAKVRILGNRGVNGIDGTESTALGIATTGKPTVLLTGDLSMLHDLNGLIVGKTHELNLTIVLFNNDGGGIF
HHLAQKGVPNFDYLFSTPHGLNFAGLAELTGLDYHLVSGYADFGQQFEASLHQSGIHLLEIKTDKDLSLALHQKYTTYEN
>Mature_559_residues
TNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGIAKAKKRPVVLVCTSGSAAAHH
FPAVLEAKMSRVPLIILTADRPAELQFVGAPQTVDQTRFFGNFVNHFENLEAPHIQKQSQTENFWTYPRKVAQRAVLSAI
SPLSGPVQINVPLRDLLVPELKSENYEKGRSKHAFKFYEGQAQVILPFDEALLSGKTLILAGANFDKDYSEALLKLAEQL
KAPILADPLSNLRNHNHPLVIDSYDAFLANNDLKTQLKADAILLFGQMPVSKRLQQFVAFNNEAEFIQVDPALDYRNPSL
TTTTMVQSDVLPFASSIKKVNSDSSYLEKWQNAQEKMRQLLEKVTYEESPFEGRYVQELQKHLEALDAQLLVSNSMEIRD
IDYWWKKADAKVRILGNRGVNGIDGTESTALGIATTGKPTVLLTGDLSMLHDLNGLIVGKTHELNLTIVLFNNDGGGIFH
HLAQKGVPNFDYLFSTPHGLNFAGLAELTGLDYHLVSGYADFGQQFEASLHQSGIHLLEIKTDKDLSLALHQKYTTYEN

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=568, Percent_Identity=26.4084507042254, Blast_Score=159, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_LACLA (Q9CHK0)

Other databases:

- EMBL:   AE005176
- PIR:   C86716
- RefSeq:   NP_266887.1
- ProteinModelPortal:   Q9CHK0
- GeneID:   1114357
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0169
- NMPDR:   fig|272623.1.peg.750
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   CLSK697379
- BioCyc:   LLAC272623:L0169-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 62525; Mature: 62394

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGI
CCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEH
AKAKKRPVVLVCTSGSAAAHHFPAVLEAKMSRVPLIILTADRPAELQFVGAPQTVDQTRF
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCHHHHHH
FGNFVNHFENLEAPHIQKQSQTENFWTYPRKVAQRAVLSAISPLSGPVQINVPLRDLLVP
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCC
ELKSENYEKGRSKHAFKFYEGQAQVILPFDEALLSGKTLILAGANFDKDYSEALLKLAEQ
HHCCCCCCCCCCCCCEEEECCCEEEEEEHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHH
LKAPILADPLSNLRNHNHPLVIDSYDAFLANNDLKTQLKADAILLFGQMPVSKRLQQFVA
HCCCHHHHHHHHHHCCCCCEEEECCHHHHCCCCCHHHHCCCEEEEECCCCHHHHHHHHHH
FNNEAEFIQVDPALDYRNPSLTTTTMVQSDVLPFASSIKKVNSDSSYLEKWQNAQEKMRQ
CCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLEKVTYEESPFEGRYVQELQKHLEALDAQLLVSNSMEIRDIDYWWKKADAKVRILGNRG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHCCCCEEEEEECCC
VNGIDGTESTALGIATTGKPTVLLTGDLSMLHDLNGLIVGKTHELNLTIVLFNNDGGGIF
CCCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCEEEECEEEEEEEEEEEECCCCHHH
HHLAQKGVPNFDYLFSTPHGLNFAGLAELTGLDYHLVSGYADFGQQFEASLHQSGIHLLE
HHHHHCCCCCCCEEECCCCCCCCHHHHHHCCCCHHHHHCHHHHHHHHHHHHHHCCEEEEE
IKTDKDLSLALHQKYTTYEN
EECCCCCEEHHHHCCCCCCC
>Mature Secondary Structure 
TNEYLAPFVDELFNLGVREAVFSPGSRSTALAMLFEEYKKYDTYVNIDERSAAFFALGI
CCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEEEH
AKAKKRPVVLVCTSGSAAAHHFPAVLEAKMSRVPLIILTADRPAELQFVGAPQTVDQTRF
HHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCHHHHHH
FGNFVNHFENLEAPHIQKQSQTENFWTYPRKVAQRAVLSAISPLSGPVQINVPLRDLLVP
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCC
ELKSENYEKGRSKHAFKFYEGQAQVILPFDEALLSGKTLILAGANFDKDYSEALLKLAEQ
HHCCCCCCCCCCCCCEEEECCCEEEEEEHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHH
LKAPILADPLSNLRNHNHPLVIDSYDAFLANNDLKTQLKADAILLFGQMPVSKRLQQFVA
HCCCHHHHHHHHHHCCCCCEEEECCHHHHCCCCCHHHHCCCEEEEECCCCHHHHHHHHHH
FNNEAEFIQVDPALDYRNPSLTTTTMVQSDVLPFASSIKKVNSDSSYLEKWQNAQEKMRQ
CCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLEKVTYEESPFEGRYVQELQKHLEALDAQLLVSNSMEIRDIDYWWKKADAKVRILGNRG
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHCCCCEEEEEECCC
VNGIDGTESTALGIATTGKPTVLLTGDLSMLHDLNGLIVGKTHELNLTIVLFNNDGGGIF
CCCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCCCEEEECEEEEEEEEEEEECCCCHHH
HHLAQKGVPNFDYLFSTPHGLNFAGLAELTGLDYHLVSGYADFGQQFEASLHQSGIHLLE
HHHHHCCCCCCCEEECCCCCCCCHHHHHHCCCCHHHHHCHHHHHHHHHHHHHHCCEEEEE
IKTDKDLSLALHQKYTTYEN
EECCCCCEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471