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The map label for this gene is blt

Identifier: 15672105

GI number: 15672105

Start: 125152

End: 126315

Strand: Direct

Name: blt

Synonym: L125116

Alternate gene names: 15672105

Gene position: 125152-126315 (Clockwise)

Preceding gene: 15672104

Following gene: 30023975

Centisome position: 5.29

GC content: 35.4

Gene sequence:

>1164_bases
ATGAAAAACAAAAAAAGCATGATGAATTTAGCAATTTCTAATCTCTTTTTAGTATTTTTGGGTGCAGGTTTAGTCATTCC
TGTTTTGCCAACCCTTAAAGAACAAATGCACTTTTCAGGAACAACAATGGGAATGATGATTTCTATTTTTGCAATTGCTC
AATTGGTTGCTTCACCAGTCGCTGGTGCTTTATCTGATAAAATTGGGCGCAAAAAACTCATTGCCATTGGAATGATTATT
TTTTCTTTTTCTGAACTTTTATTTGGTTTAGCACAAGCGAAAACAGGTTTTTATATTTCGCGAGCGCTAGGGGGTGTCGC
AGCTGCGATGCTCATGCCTTCAGTTACTGCTTACGTGGCTGATATGACCACAATTGCCGAACGTCCTAAAGCGATGGGAC
TTGTTTCAGCAGCAATCAGTGGTGGCTTTATTATTGGTCCTGGAGTGGGTGGTTTTATTGCCCATTTTGGAATACGTGTT
CCTTTTTATGTTGCAGCAATTTTAGCTTTCCTTGGTTTTATTTTAACAATCACAATTTTAAAAGAACCAGAGCGCACAAT
TGAAAGTCATCAAGAAATCGAAAAAGTTTCATTTCTTGATATTCTCAAAAATCCATTATTTGGTTCACTCTTTATTATAA
TTTTGATTTCATCATTTGGTTTACAGGCCTTTGAGTCAATTTATAGTATCATGGCAAGTATTAACTTTGGTTTTAGTATG
AGTGAAATTGCTTTGGTAATTACAGTGAGTGGAATTTTAGCTCTCTTTTTCCAACTTTTCTTATTTGATGCAATTGTCAA
TAAAATTGGTGAACTTGGCTTGATTCAATTAACTTTCTTTGCCAGTGCAATCTTTATTGCTGTTATTGCCTTTACAAAAT
CAAATTTAGTCGTTGCTCTTTCTACATTTGTTGTTTTTCTGGCCTTTGATTTATTTAGACCGGCTGTAACAACCTATCTT
TCAAAGCATGCGGGCAATCGGCAAGGGGCAATCAATGGTTTGAACTCAGCCTTTACGAGTTTTGGAAATATTTTAGGGCC
AATGGCCGCGGGTTATATGTTTGACCTTAACCATTTATTTCCATATTATATCTCTGCGATTATTCTACTGGGAACCGGAT
TTTTATCACTATTCTTAAACAGAAAAAATTTCAGTAAAATCTAA

Upstream 100 bases:

>100_bases
TTTTAGTATATAGATTTCATGATATAATAAGAGAATTGGTAAAAAATAAAAGAAATAGCAATAGAATAATCTATTTGCTA
TTTTTAATGAGGAGGAAAAA

Downstream 100 bases:

>100_bases
ATAAAACTTTCAATTTTATTATATTTATAACTATTACCTTGCAAAAAGTGGTAGTTTTTTTCATCAACGAAAATTTTTTT
GAATAAAAAAACCTCCTGAC

Product: multidrug efflux transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2 [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLVASPVAGALSDKIGRKKLIAIGMII
FSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVADMTTIAERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRV
PFYVAAILAFLGFILTITILKEPERTIESHQEIEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
SEIALVITVSGILALFFQLFLFDAIVNKIGELGLIQLTFFASAIFIAVIAFTKSNLVVALSTFVVFLAFDLFRPAVTTYL
SKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDLNHLFPYYISAIILLGTGFLSLFLNRKNFSKI

Sequences:

>Translated_387_residues
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLVASPVAGALSDKIGRKKLIAIGMII
FSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVADMTTIAERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRV
PFYVAAILAFLGFILTITILKEPERTIESHQEIEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
SEIALVITVSGILALFFQLFLFDAIVNKIGELGLIQLTFFASAIFIAVIAFTKSNLVVALSTFVVFLAFDLFRPAVTTYL
SKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDLNHLFPYYISAIILLGTGFLSLFLNRKNFSKI
>Mature_387_residues
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLVASPVAGALSDKIGRKKLIAIGMII
FSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVADMTTIAERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRV
PFYVAAILAFLGFILTITILKEPERTIESHQEIEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
SEIALVITVSGILALFFQLFLFDAIVNKIGELGLIQLTFFASAIFIAVIAFTKSNLVVALSTFVVFLAFDLFRPAVTTYL
SKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDLNHLFPYYISAIILLGTGFLSLFLNRKNFSKI

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=357, Percent_Identity=27.4509803921569, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081738, Length=359, Percent_Identity=26.7409470752089, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137160, Length=228, Percent_Identity=25.8771929824561, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221513297, Length=358, Percent_Identity=25.9776536312849, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41672; Mature: 41672

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLVASPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AGALSDKIGRKKLIAIGMIIFSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DMTTIAERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRVPFYVAAILAFLGFILTITIL
HHHHHHHCHHHHHHHHHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
KEPERTIESHQEIEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCH
SEIALVITVSGILALFFQLFLFDAIVNKIGELGLIQLTFFASAIFIAVIAFTKSNLVVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
STFVVFLAFDLFRPAVTTYLSKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDLNHLF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PYYISAIILLGTGFLSLFLNRKNFSKI
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKNKKSMMNLAISNLFLVFLGAGLVIPVLPTLKEQMHFSGTTMGMMISIFAIAQLVASPV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AGALSDKIGRKKLIAIGMIIFSFSELLFGLAQAKTGFYISRALGGVAAAMLMPSVTAYVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DMTTIAERPKAMGLVSAAISGGFIIGPGVGGFIAHFGIRVPFYVAAILAFLGFILTITIL
HHHHHHHCHHHHHHHHHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
KEPERTIESHQEIEKVSFLDILKNPLFGSLFIIILISSFGLQAFESIYSIMASINFGFSM
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCH
SEIALVITVSGILALFFQLFLFDAIVNKIGELGLIQLTFFASAIFIAVIAFTKSNLVVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
STFVVFLAFDLFRPAVTTYLSKHAGNRQGAINGLNSAFTSFGNILGPMAAGYMFDLNHLF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PYYISAIILLGTGFLSLFLNRKNFSKI
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377 [H]