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The map label for this gene is argG

Identifier: 30023975

GI number: 30023975

Start: 126757

End: 127950

Strand: Direct

Name: argG

Synonym: L126739

Alternate gene names: 30023975

Gene position: 126757-127950 (Clockwise)

Preceding gene: 15672105

Following gene: 15672107

Centisome position: 5.36

GC content: 37.6

Gene sequence:

>1194_bases
ATGGGAAACAAAAAAATTGTCTTGGCATATTCGGGTGGATTGGATACAAGTGTTGCTGTAAAATGGTTGACTGATAAAGG
ATTTGACGTCATTGCTGACTGCATGGACGTTGGTGAAGGAAAAGACTTGAATTTTATTCATGATAAGGCGCTTCAAGTAG
GGGCAGTGGAATCTGTTGTTTTGGATTGCAAGGAAGAATTCGCTAAAATATTTGTTGGAGCTGCACTCAAAGGAAATTTA
ATGTATGAAAATAAATATCCTTTAGTATCCGCTTTGAGTCGCCCTTTGATTGCTCAAAAATTAGTAGAAGTAGCCAAAGA
AAAAGGGGCAACGGCTATTGCTCATGGTTGTACAGGAAAAGGAAATGACCAAGTGCGTTTTGAAGTAGCCATCCATTCCC
TTGCGCCTGAGCTTGAAGTCATTGCTCCAGTTCGTGAATGGCATTGGGCTCGTGAAGAAGAAATCGAATATGCCAATCAA
AATGGTGTTCCTATTCCTGCTGATTTAGACAATCCCTACTCAATTGACATGAATTTATGGGGACGTGCGATTGAAGCTGG
TGTTCTTGAAAATCCTTGGAATACTTGTCCAGAGGATGCTTTCTTTATGACTAATTCTGTAGAGAACGCTCCAAACGAAG
CTGAATTTATCGAGGTTGAGTTTAAAGAAGGATTACCAATCGCCTTAAATGGAAAAAGCTTAGAATTGCATGAAATCATT
AAAAAAGTGAATATTATTGCAGGAAAACATGGAATTGGTCGGATTGACCATATTGAAAATCGCCTGGTTGGGATTAAATC
CCGTGAATTTTATGAATGTCCTGCTGCCATTACTTTATTAAAAGCACATAAAGATTTGGAAGATTTAACTTTCGTTCGAG
AACTTGCACATTTTAAACCAGTTCTTGAAAATGAATTAGCAAACTTGATTTACAATGGTTTGTGGTTCAATCCTGCTACT
AAGGCTTTGATCGCTTATCTTGATGAAACTCAAAAAGTAGTTAACGGGATTGTAAAAATTAAATTATATAAAGGTTTAGC
AACACCAGTGGGACGTAAATCAACTAATTCGCTTTACAGCGAAAAATTAGCAACATATACTGCTGCAGATGAGTTTGACC
AAGCAGCGGCAGTTGGATTTATTAAATTGTGGGGTCTACCTACTCAAGTCAATGCTCAAGTAAATCTCAAATAA

Upstream 100 bases:

>100_bases
TGCTAGAATGAAAATAAAACGCATATTTATACAAAAATGTTGATAAATGAATAAATATGCTGTACAATTGCATTTATATT
AATTTGAAAGAAGAAAAATG

Downstream 100 bases:

>100_bases
TAGCTATAGGAAAATTTGGAAAGTAAGAGAATGGTAGAAAAACTTTGGGGAGGACGTTTTGAGGCGTCACTAGATAAACA
AACGGAAGAATTTGGAGCAT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MGNKKIVLAYSGGLDTSVAVKWLTDKGFDVIADCMDVGEGKDLNFIHDKALQVGAVESVVLDCKEEFAKIFVGAALKGNL
MYENKYPLVSALSRPLIAQKLVEVAKEKGATAIAHGCTGKGNDQVRFEVAIHSLAPELEVIAPVREWHWAREEEIEYANQ
NGVPIPADLDNPYSIDMNLWGRAIEAGVLENPWNTCPEDAFFMTNSVENAPNEAEFIEVEFKEGLPIALNGKSLELHEII
KKVNIIAGKHGIGRIDHIENRLVGIKSREFYECPAAITLLKAHKDLEDLTFVRELAHFKPVLENELANLIYNGLWFNPAT
KALIAYLDETQKVVNGIVKIKLYKGLATPVGRKSTNSLYSEKLATYTAADEFDQAAAVGFIKLWGLPTQVNAQVNLK

Sequences:

>Translated_397_residues
MGNKKIVLAYSGGLDTSVAVKWLTDKGFDVIADCMDVGEGKDLNFIHDKALQVGAVESVVLDCKEEFAKIFVGAALKGNL
MYENKYPLVSALSRPLIAQKLVEVAKEKGATAIAHGCTGKGNDQVRFEVAIHSLAPELEVIAPVREWHWAREEEIEYANQ
NGVPIPADLDNPYSIDMNLWGRAIEAGVLENPWNTCPEDAFFMTNSVENAPNEAEFIEVEFKEGLPIALNGKSLELHEII
KKVNIIAGKHGIGRIDHIENRLVGIKSREFYECPAAITLLKAHKDLEDLTFVRELAHFKPVLENELANLIYNGLWFNPAT
KALIAYLDETQKVVNGIVKIKLYKGLATPVGRKSTNSLYSEKLATYTAADEFDQAAAVGFIKLWGLPTQVNAQVNLK
>Mature_396_residues
GNKKIVLAYSGGLDTSVAVKWLTDKGFDVIADCMDVGEGKDLNFIHDKALQVGAVESVVLDCKEEFAKIFVGAALKGNLM
YENKYPLVSALSRPLIAQKLVEVAKEKGATAIAHGCTGKGNDQVRFEVAIHSLAPELEVIAPVREWHWAREEEIEYANQN
GVPIPADLDNPYSIDMNLWGRAIEAGVLENPWNTCPEDAFFMTNSVENAPNEAEFIEVEFKEGLPIALNGKSLELHEIIK
KVNIIAGKHGIGRIDHIENRLVGIKSREFYECPAAITLLKAHKDLEDLTFVRELAHFKPVLENELANLIYNGLWFNPATK
ALIAYLDETQKVVNGIVKIKLYKGLATPVGRKSTNSLYSEKLATYTAADEFDQAAAVGFIKLWGLPTQVNAQVNLK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=391, Percent_Identity=41.1764705882353, Blast_Score=297, Evalue=9e-81,
Organism=Homo sapiens, GI16950633, Length=391, Percent_Identity=41.1764705882353, Blast_Score=297, Evalue=9e-81,
Organism=Escherichia coli, GI1789563, Length=377, Percent_Identity=30.2387267904509, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=402, Percent_Identity=35.5721393034826, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI21358151, Length=400, Percent_Identity=37.5, Blast_Score=277, Evalue=1e-74,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_LACLA (P57799)

Other databases:

- EMBL:   AE005176
- PIR:   D86640
- RefSeq:   NP_266280.2
- ProteinModelPortal:   P57799
- SMR:   P57799
- GeneID:   1113730
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L126739
- NMPDR:   fig|272623.1.peg.131
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   LLAC272623:L126739-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 43830; Mature: 43699

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 87-87 BINDING 117-117 BINDING 119-119 BINDING 123-123 BINDING 123-123 BINDING 124-124 BINDING 127-127 BINDING 175-175 BINDING 260-260 BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKKIVLAYSGGLDTSVAVKWLTDKGFDVIADCMDVGEGKDLNFIHDKALQVGAVESVV
CCCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LDCKEEFAKIFVGAALKGNLMYENKYPLVSALSRPLIAQKLVEVAKEKGATAIAHGCTGK
HHHHHHHHHHHHHHEEECCEEECCCCCHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFEVAIHSLAPELEVIAPVREWHWAREEEIEYANQNGVPIPADLDNPYSIDMNLW
CCCEEEEEEEEEHHCCCHHEECCHHHHCCCCHHHHHHCCCCCCCCCCCCCCCEEEEHHHH
GRAIEAGVLENPWNTCPEDAFFMTNSVENAPNEAEFIEVEFKEGLPIALNGKSLELHEII
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEECCCCCHHHHHH
KKVNIIAGKHGIGRIDHIENRLVGIKSREFYECPAAITLLKAHKDLEDLTFVRELAHFKP
HHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHH
VLENELANLIYNGLWFNPATKALIAYLDETQKVVNGIVKIKLYKGLATPVGRKSTNSLYS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHH
EKLATYTAADEFDQAAAVGFIKLWGLPTQVNAQVNLK
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECC
>Mature Secondary Structure 
GNKKIVLAYSGGLDTSVAVKWLTDKGFDVIADCMDVGEGKDLNFIHDKALQVGAVESVV
CCCEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LDCKEEFAKIFVGAALKGNLMYENKYPLVSALSRPLIAQKLVEVAKEKGATAIAHGCTGK
HHHHHHHHHHHHHHEEECCEEECCCCCHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFEVAIHSLAPELEVIAPVREWHWAREEEIEYANQNGVPIPADLDNPYSIDMNLW
CCCEEEEEEEEEHHCCCHHEECCHHHHCCCCHHHHHHCCCCCCCCCCCCCCCEEEEHHHH
GRAIEAGVLENPWNTCPEDAFFMTNSVENAPNEAEFIEVEFKEGLPIALNGKSLELHEII
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCCEEEECCCCCHHHHHH
KKVNIIAGKHGIGRIDHIENRLVGIKSREFYECPAAITLLKAHKDLEDLTFVRELAHFKP
HHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHH
VLENELANLIYNGLWFNPATKALIAYLDETQKVVNGIVKIKLYKGLATPVGRKSTNSLYS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHH
EKLATYTAADEFDQAAAVGFIKLWGLPTQVNAQVNLK
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471