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Identifier: 15834757

GI number: 15834757

Start: 167900

End: 169258

Strand: Direct

Name: Not Available

Synonym: TC0137

Alternate gene names: 15834757

Gene position: 167900-169258 (Clockwise)

Preceding gene: 15834753

Following gene: 15834758

Centisome position: 15.65

GC content: 41.35

Gene sequence:

>1359_bases
TTGGTTATGCGCCCTATTTTATTAAAAGAGTGGTCTTCTTTGTTATTAGATATTGACATTCCTTGCTCAGGGAAGAAAGT
AACGGGAGTCGCTATTGACAGCCGGCTTGTATTGCCAGGGGATGTATTTTTTGCTCTTCCAGGGAATCGAACTACTGGGC
ACCTATTTTTAAAGCAGGCTGCGCAATCCGGAGCTGTTGCTGCTGTAGTAGCGAGTGATTATCGTGGACCAGATTATGGG
TTACAGTTGTTACATGTGGCAGATCCTAGAGAAGCTTTAAGAGCGGCTGGACGAACTCAGGGAGCTCTTTTCCATGGAGA
AGTGATAGGGATAACTGGCTCTGTAGGGAAAACGACAACAAAAAGTTTTACCCAACAATTACTTTCTCCTTTTTACAAAG
TCTTTGCAAGTCCGAAAAGTTATAATTCTCAGCTCACTCTACCCCTAAGTATATTAACGGCCGATGGAGATGAGGATTTT
CTTGTTTTAGAGATGGGAGTTTCTGAACCCAATAATATGAAGGATCTTTTAGATGTAATTGAGCCTTCTATTGGAGTGAT
TACTCACATCGACGTTCAGCATGCAATGAATTTTGTGGATAAAGGGGCTCAAGGTATTGCTGAAGAGAAGAGTTTGTTGT
TAGAGAGATGCGGGATTCATCTATTGCCCAGAGATTCTTCATGGTTTAACTTTTTCATGAAAAAGAATGCTGCAGCTGAT
CAGTTTTCTTTTTCTATGAATAATGAAACAGCAGATTTTTACTATCGAGCCATTCATTCCGAAGGGGTCCTCATTAGTGC
TCCCGATGGGGATATTGAGCTGCCTGTAGTGTTCCCTTATACTCCCGCTTATATGAATTTTATTATTGCTGTAGCTTTAG
CTTGGCTTACTAACGTTCCAATGGATCGTTTAGAACAGGCAAGTCAATCTTTATTTTTGCCATCTATGCGCTTTGAACAA
CAGGAACACAATGGTATTCGAATTATTAATGATGCATACAATGCAAGCCCAGATGCAATGCTAGCTGCTTTAGATGCTGT
CCCTATCCCCTCTGAGGGAGGTAAGACAGTATTTATCTTGGGCCATATGGCGGAGTTAGGAAGATATTCGGACGAATGTC
ATATTGCTGTAGCAAGAAAAGCTGTGACAAAAGCACACGTAGTTTTTTTCGTTGGAGAAAAGTGGTCCCCTGTTAGAGAG
GTTGTTTCTGATGCAAATTGTCAGATAGAGTTTTACGGTTCTGTTAGCGAGATTATGCATGTTGTTAAATCTTTAGTGCG
ACGGGGAGATGTTGTTCTGCTCAAAGGATCGCGTAGTTTGGAATTGGAGACTTTGTTGCCATGTTTTTCCATCTCGTGA

Upstream 100 bases:

>100_bases
AAGAGATCTCTTTAGGAGTTTATTTTGTTAGTGTGCTCTCAAAGATTATTTCTTATTTTGAGAATTTGGAGTTGTTTGTT
TTCAGTGTGAAGATAAGGGA

Downstream 100 bases:

>100_bases
GTGGAAGACAGCAGTTTCTTTTTAAAATAAGAAAAGAAATAGTTAGTTTTTGATGCTTAAGTTTAAGATAGAGAACTCCT
TATAGTCGACACTCTCATAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase MurF, putative

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MVMRPILLKEWSSLLLDIDIPCSGKKVTGVAIDSRLVLPGDVFFALPGNRTTGHLFLKQAAQSGAVAAVVASDYRGPDYG
LQLLHVADPREALRAAGRTQGALFHGEVIGITGSVGKTTTKSFTQQLLSPFYKVFASPKSYNSQLTLPLSILTADGDEDF
LVLEMGVSEPNNMKDLLDVIEPSIGVITHIDVQHAMNFVDKGAQGIAEEKSLLLERCGIHLLPRDSSWFNFFMKKNAAAD
QFSFSMNNETADFYYRAIHSEGVLISAPDGDIELPVVFPYTPAYMNFIIAVALAWLTNVPMDRLEQASQSLFLPSMRFEQ
QEHNGIRIINDAYNASPDAMLAALDAVPIPSEGGKTVFILGHMAELGRYSDECHIAVARKAVTKAHVVFFVGEKWSPVRE
VVSDANCQIEFYGSVSEIMHVVKSLVRRGDVVLLKGSRSLELETLLPCFSIS

Sequences:

>Translated_452_residues
MVMRPILLKEWSSLLLDIDIPCSGKKVTGVAIDSRLVLPGDVFFALPGNRTTGHLFLKQAAQSGAVAAVVASDYRGPDYG
LQLLHVADPREALRAAGRTQGALFHGEVIGITGSVGKTTTKSFTQQLLSPFYKVFASPKSYNSQLTLPLSILTADGDEDF
LVLEMGVSEPNNMKDLLDVIEPSIGVITHIDVQHAMNFVDKGAQGIAEEKSLLLERCGIHLLPRDSSWFNFFMKKNAAAD
QFSFSMNNETADFYYRAIHSEGVLISAPDGDIELPVVFPYTPAYMNFIIAVALAWLTNVPMDRLEQASQSLFLPSMRFEQ
QEHNGIRIINDAYNASPDAMLAALDAVPIPSEGGKTVFILGHMAELGRYSDECHIAVARKAVTKAHVVFFVGEKWSPVRE
VVSDANCQIEFYGSVSEIMHVVKSLVRRGDVVLLKGSRSLELETLLPCFSIS
>Mature_452_residues
MVMRPILLKEWSSLLLDIDIPCSGKKVTGVAIDSRLVLPGDVFFALPGNRTTGHLFLKQAAQSGAVAAVVASDYRGPDYG
LQLLHVADPREALRAAGRTQGALFHGEVIGITGSVGKTTTKSFTQQLLSPFYKVFASPKSYNSQLTLPLSILTADGDEDF
LVLEMGVSEPNNMKDLLDVIEPSIGVITHIDVQHAMNFVDKGAQGIAEEKSLLLERCGIHLLPRDSSWFNFFMKKNAAAD
QFSFSMNNETADFYYRAIHSEGVLISAPDGDIELPVVFPYTPAYMNFIIAVALAWLTNVPMDRLEQASQSLFLPSMRFEQ
QEHNGIRIINDAYNASPDAMLAALDAVPIPSEGGKTVFILGHMAELGRYSDECHIAVARKAVTKAHVVFFVGEKWSPVRE
VVSDANCQIEFYGSVSEIMHVVKSLVRRGDVVLLKGSRSLELETLLPCFSIS

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=433, Percent_Identity=27.2517321016166, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49525; Mature: 49525

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMRPILLKEWSSLLLDIDIPCSGKKVTGVAIDSRLVLPGDVFFALPGNRTTGHLFLKQA
CCCCCHHHHHHCCEEEEEECCCCCCEEEEEEECCEEEECCCEEEEECCCCCCHHHHHHHH
AQSGAVAAVVASDYRGPDYGLQLLHVADPREALRAAGRTQGALFHGEVIGITGSVGKTTT
HCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEECEEEEEECCCCCCHH
KSFTQQLLSPFYKVFASPKSYNSQLTLPLSILTADGDEDFLVLEMGVSEPNNMKDLLDVI
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHH
EPSIGVITHIDVQHAMNFVDKGAQGIAEEKSLLLERCGIHLLPRDSSWFNFFMKKNAAAD
CCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCC
QFSFSMNNETADFYYRAIHSEGVLISAPDGDIELPVVFPYTPAYMNFIIAVALAWLTNVP
CEEEECCCCCHHHHHEEECCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC
MDRLEQASQSLFLPSMRFEQQEHNGIRIINDAYNASPDAMLAALDAVPIPSEGGKTVFIL
HHHHHHHHHHEECCCHHHCHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEE
GHMAELGRYSDECHIAVARKAVTKAHVVFFVGEKWSPVREVVSDANCQIEFYGSVSEIMH
ECHHHHCCCCCCCEEEHHHHHHCEEEEEEEECCCCCHHHHHHCCCCCEEEECCCHHHHHH
VVKSLVRRGDVVLLKGSRSLELETLLPCFSIS
HHHHHHHCCCEEEEECCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MVMRPILLKEWSSLLLDIDIPCSGKKVTGVAIDSRLVLPGDVFFALPGNRTTGHLFLKQA
CCCCCHHHHHHCCEEEEEECCCCCCEEEEEEECCEEEECCCEEEEECCCCCCHHHHHHHH
AQSGAVAAVVASDYRGPDYGLQLLHVADPREALRAAGRTQGALFHGEVIGITGSVGKTTT
HCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEECEEEEEECCCCCCHH
KSFTQQLLSPFYKVFASPKSYNSQLTLPLSILTADGDEDFLVLEMGVSEPNNMKDLLDVI
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHH
EPSIGVITHIDVQHAMNFVDKGAQGIAEEKSLLLERCGIHLLPRDSSWFNFFMKKNAAAD
CCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCC
QFSFSMNNETADFYYRAIHSEGVLISAPDGDIELPVVFPYTPAYMNFIIAVALAWLTNVP
CEEEECCCCCHHHHHEEECCCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCC
MDRLEQASQSLFLPSMRFEQQEHNGIRIINDAYNASPDAMLAALDAVPIPSEGGKTVFIL
HHHHHHHHHHEECCCHHHCHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEE
GHMAELGRYSDECHIAVARKAVTKAHVVFFVGEKWSPVREVVSDANCQIEFYGSVSEIMH
ECHHHHCCCCCCCEEEHHHHHHCEEEEEEEECCCCCHHHHHHCCCCCEEEECCCHHHHHH
VVKSLVRRGDVVLLKGSRSLELETLLPCFSIS
HHHHHHHCCCEEEEECCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]