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The map label for this gene is pyrI

Identifier: 16120220

GI number: 16120220

Start: 240304

End: 240783

Strand: Direct

Name: pyrI

Synonym: VNG6311G

Alternate gene names: 16120220

Gene position: 240304-240783 (Clockwise)

Preceding gene: 16120219

Following gene: 16120221

Centisome position: 65.76

GC content: 61.46

Gene sequence:

>480_bases
ATGGGGGTGTCGACGATGAGTAGCGACCAACACCTCCAGGTCAGCAAAATCCAGGCCGGAACCGTCATCGACCACATCCC
GGCCGGGCAGGCGCTGCAAGTGCTTCAGATCCTCGGGACCAACGGAGCAAGCGACGACCAGATCACCGTCGGGATGAACG
TCACCTCGGAACGCCACCACCGCAAGGACATCGTCAAGATCGAGGGTCGAGAGCTGAGCCAGGACGAAGTCGACGTCCTG
TCGCTGATCGCGCCCGACGCAACGATCAACATCGTCCGTGACTACGAGGTCGACGAGAAACGTCGCGTCGACCGTCCCGA
CGTCGTTGCGGGTGTGCTCTCCTGTCCGAACCCCAACTGCATCACCGCCAACGACGAGCCCGTGCGATCCGGGTTCGACG
TGCTCGACGACGGGATGCGATGTCAGTACTGCGAACAGATCGTCAGTGAGGACATCCCGTCACTGATAGATCCGGAGTAG

Upstream 100 bases:

>100_bases
GCACCCGAAATCGACGACACCGACCACGCGGCCTACTTCGAGCAGGCACACAACGGGGTGCCGGTTCGCATGGCACTGCT
GGATCTGGTGCTGTCCGCCG

Downstream 100 bases:

>100_bases
GCGGCCGTACTCGTGGTTTTTCGTGGCTTTCTCTCGTTATTGTGTTGATTACTGTTCGACTGCTGCCGGGCGAACCACTG
GCCCGGATGTAGCAGAATTT

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MGVSTMSSDQHLQVSKIQAGTVIDHIPAGQALQVLQILGTNGASDDQITVGMNVTSERHHRKDIVKIEGRELSQDEVDVL
SLIAPDATINIVRDYEVDEKRRVDRPDVVAGVLSCPNPNCITANDEPVRSGFDVLDDGMRCQYCEQIVSEDIPSLIDPE

Sequences:

>Translated_159_residues
MGVSTMSSDQHLQVSKIQAGTVIDHIPAGQALQVLQILGTNGASDDQITVGMNVTSERHHRKDIVKIEGRELSQDEVDVL
SLIAPDATINIVRDYEVDEKRRVDRPDVVAGVLSCPNPNCITANDEPVRSGFDVLDDGMRCQYCEQIVSEDIPSLIDPE
>Mature_158_residues
GVSTMSSDQHLQVSKIQAGTVIDHIPAGQALQVLQILGTNGASDDQITVGMNVTSERHHRKDIVKIEGRELSQDEVDVLS
LIAPDATINIVRDYEVDEKRRVDRPDVVAGVLSCPNPNCITANDEPVRSGFDVLDDGMRCQYCEQIVSEDIPSLIDPE

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase [H]

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family [H]

Homologues:

Organism=Escherichia coli, GI1790692, Length=151, Percent_Identity=43.7086092715232, Blast_Score=122, Evalue=1e-29,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- ProDom:   PD006194 [H]

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C [H]

EC number: 2.1.3.2

Molecular weight: Translated: 17394; Mature: 17262

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVSTMSSDQHLQVSKIQAGTVIDHIPAGQALQVLQILGTNGASDDQITVGMNVTSERHH
CCCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHH
RKDIVKIEGRELSQDEVDVLSLIAPDATINIVRDYEVDEKRRVDRPDVVAGVLSCPNPNC
CCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCHHHHHHHCCCCCCE
ITANDEPVRSGFDVLDDGMRCQYCEQIVSEDIPSLIDPE
ECCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
GVSTMSSDQHLQVSKIQAGTVIDHIPAGQALQVLQILGTNGASDDQITVGMNVTSERHH
CCCCCCCCCCEEEEEECCCCEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHH
RKDIVKIEGRELSQDEVDVLSLIAPDATINIVRDYEVDEKRRVDRPDVVAGVLSCPNPNC
CCCEEEECCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCHHHHHHHCCCCCCE
ITANDEPVRSGFDVLDDGMRCQYCEQIVSEDIPSLIDPE
ECCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11016950 [H]