Definition Halobacterium sp. NRC-1 plasmid pNRC200, complete sequence.
Accession NC_002608
Length 365,425

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The map label for this gene is argS

Identifier: 16120221

GI number: 16120221

Start: 240914

End: 242809

Strand: Direct

Name: argS

Synonym: VNG6312G

Alternate gene names: 16120221

Gene position: 240914-242809 (Clockwise)

Preceding gene: 16120220

Following gene: 16120227

Centisome position: 65.93

GC content: 62.39

Gene sequence:

>1896_bases
ATGCTTTATAACCTTCGGCAGGAACTGCTCGCTGGAATCCGGGCTGCCACCTCTGACGCGGGGTACGACTATGAAGTCGA
CCAGTCCGCGATCGAACTCGAAGACATCACCGATGAGGAGAAAGGCGAGTTCTCCTCACCGATCTCGTTCTCGATCGCGG
CCGCGGCCGGAGCGCCCCCGGTCGATGTCGCGGCGGCGATCGCCGACGCCCACCGCTCCAACGGGCTGCCGGCTGAAGTC
GAGGCGGTGACCGTCGAAGGCGGCCACATCAACTACCACGCCGACACGACCGATCTGGCAGACGCGACGCTGTCGACGAT
CCTCCGCGACGGTTCGGAGTACGGCACGCGGACGGACGCCGACCCGGACACCATCCTCGCCGACGTGTCCTCGCCCAACA
TCGCCAAGCCGCTGCACGTCGGCCACTTGCGGAACACGATCCTCAGCGATGCAGTGATGAACATCCTGGAGGCGCGCGGC
CACGACGTCACGCGGGACAACCATCTCGGGGATTGGGGCGTGCAGTTCGGGAACCTGATGCACGAGTACACCGAGTTCGG
CGACGAAGCGACGCTGGAAGACGACGCCATCGAACACCTCCTGGATCTCTACCAGCAGTTCGAGCAGCGGGACTCGATGC
TCGCGGATCTCGAAGACGATGAGACCGTGACCGACCAGTTCGCCGACGCCGTCACCGAGGAACGTGACTACCACGCCGAC
AGCGGCAAGGAGTGGTTCACCCGGCTCGAGCAGGGCGACGAGGACGCGACTGCCCTCTGGGAGCGGTTCCGTACCGTGAG
CATCGATCGCTTCAAGCAGACCTACGACGACCTCGATGTCGCATTCGACGTTTGGAACGGGGAGAGCTTCTACGCTCAGG
AGGGCTGGAACGACGTCATCATCGAGAAGGCGATCGAGAACGACGTCGCGATGCGCGGCGAGGGCGAGTCGGTGTACATC
CCGGTGTACCCTGACGACTACGAGAACGTCGGCGACCCGCAAGCGGCAGACGTGGACGCCTCGCTCGATCGCGCACGCCA
GATGCGGGAGGCCAACGACGACCTCGAAGACGCCGACTTCGATCCGTTCTACATCGTCAAGTCCGATGGATCGACGCTGT
ACGGAACCCGCGACCTGGCGACGATCGAGTACCGGATCGAGGAATACGACGCCGATCAGTCGGTCTACGTGGTGGCAAAC
GAACAGAACCAGTACTTCCAACAGCTGTTCGTCGCTGCCCGAAAGATGGGATACAACGACATCAAACTTAAGCACATCGA
TTACGGGCTGATCAGCCTCCCCGAAGGGAGCATGTCGACACGGAAAGGACAGATCATCACCGCCCGGGAAGTCCTTGATC
GTGCCCAGGACCGCGCCGAGGAGATCATCGCGGAGAAAGGCCGAATCGACGACGCCGAAGCCCAATCGGTCGCAACCAAG
ATCGCGCTCGCAACCATCAAATACGAGATGGTGGCGGCCAAACGCGAACGCGACACGACGTTCGACATCGACGAGTCGGT
TGCACTGGAGGGCGACACCGGACCGTACGTTCAGTACGCGGCAACCCGTGGCTACAGCATTCTGGACGGAGCGGACGCCG
CTCCCGAGATCGACGACCTCGATCCATCGGTGTTCAACGACACCGATGTGGAGCTGCTGTTCGAGCTGGCCCGCTACCCA
CTCGTCCTGGAACGCTGTGAGGAACGGTACGACGCGGCACCGCTTGCCCACTACCTCCTCCAGCTCGCACACGTGTTCAA
CTCCTTCTACCACAAGAACGCGGTCTTGGACGCCGAAAACGCACGCACGGAGCGCCTCCTGCTGACGAAGGCAACGACAC
AGATCTTCGACAACGGCCTCGGCCTGCTCGGCATCGACGTGCTCGAAGAGATGTAA

Upstream 100 bases:

>100_bases
CTCTCGTTATTGTGTTGATTACTGTTCGACTGCTGCCGGGCGAACCACTGGCCCGGATGTAGCAGAATTTATCGTTCTGG
AGGGTGAAGGCTCGCATATA

Downstream 100 bases:

>100_bases
CCGCGCTGGACATCGTTGAATCGGTTTTGAGGTCCGTGAAACTCGGTTGGCAGCTACTACGATCGCTGTAAGAACGACAC
AGGAGCGCCGGTGTCTTCCG

Product: arginine-tRNA synthetase

Products: NA

Alternate protein names: Arginine--tRNA ligase; ArgRS

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MLYNLRQELLAGIRAATSDAGYDYEVDQSAIELEDITDEEKGEFSSPISFSIAAAAGAPPVDVAAAIADAHRSNGLPAEV
EAVTVEGGHINYHADTTDLADATLSTILRDGSEYGTRTDADPDTILADVSSPNIAKPLHVGHLRNTILSDAVMNILEARG
HDVTRDNHLGDWGVQFGNLMHEYTEFGDEATLEDDAIEHLLDLYQQFEQRDSMLADLEDDETVTDQFADAVTEERDYHAD
SGKEWFTRLEQGDEDATALWERFRTVSIDRFKQTYDDLDVAFDVWNGESFYAQEGWNDVIIEKAIENDVAMRGEGESVYI
PVYPDDYENVGDPQAADVDASLDRARQMREANDDLEDADFDPFYIVKSDGSTLYGTRDLATIEYRIEEYDADQSVYVVAN
EQNQYFQQLFVAARKMGYNDIKLKHIDYGLISLPEGSMSTRKGQIITAREVLDRAQDRAEEIIAEKGRIDDAEAQSVATK
IALATIKYEMVAAKRERDTTFDIDESVALEGDTGPYVQYAATRGYSILDGADAAPEIDDLDPSVFNDTDVELLFELARYP
LVLERCEERYDAAPLAHYLLQLAHVFNSFYHKNAVLDAENARTERLLLTKATTQIFDNGLGLLGIDVLEEM

Sequences:

>Translated_631_residues
MLYNLRQELLAGIRAATSDAGYDYEVDQSAIELEDITDEEKGEFSSPISFSIAAAAGAPPVDVAAAIADAHRSNGLPAEV
EAVTVEGGHINYHADTTDLADATLSTILRDGSEYGTRTDADPDTILADVSSPNIAKPLHVGHLRNTILSDAVMNILEARG
HDVTRDNHLGDWGVQFGNLMHEYTEFGDEATLEDDAIEHLLDLYQQFEQRDSMLADLEDDETVTDQFADAVTEERDYHAD
SGKEWFTRLEQGDEDATALWERFRTVSIDRFKQTYDDLDVAFDVWNGESFYAQEGWNDVIIEKAIENDVAMRGEGESVYI
PVYPDDYENVGDPQAADVDASLDRARQMREANDDLEDADFDPFYIVKSDGSTLYGTRDLATIEYRIEEYDADQSVYVVAN
EQNQYFQQLFVAARKMGYNDIKLKHIDYGLISLPEGSMSTRKGQIITAREVLDRAQDRAEEIIAEKGRIDDAEAQSVATK
IALATIKYEMVAAKRERDTTFDIDESVALEGDTGPYVQYAATRGYSILDGADAAPEIDDLDPSVFNDTDVELLFELARYP
LVLERCEERYDAAPLAHYLLQLAHVFNSFYHKNAVLDAENARTERLLLTKATTQIFDNGLGLLGIDVLEEM
>Mature_631_residues
MLYNLRQELLAGIRAATSDAGYDYEVDQSAIELEDITDEEKGEFSSPISFSIAAAAGAPPVDVAAAIADAHRSNGLPAEV
EAVTVEGGHINYHADTTDLADATLSTILRDGSEYGTRTDADPDTILADVSSPNIAKPLHVGHLRNTILSDAVMNILEARG
HDVTRDNHLGDWGVQFGNLMHEYTEFGDEATLEDDAIEHLLDLYQQFEQRDSMLADLEDDETVTDQFADAVTEERDYHAD
SGKEWFTRLEQGDEDATALWERFRTVSIDRFKQTYDDLDVAFDVWNGESFYAQEGWNDVIIEKAIENDVAMRGEGESVYI
PVYPDDYENVGDPQAADVDASLDRARQMREANDDLEDADFDPFYIVKSDGSTLYGTRDLATIEYRIEEYDADQSVYVVAN
EQNQYFQQLFVAARKMGYNDIKLKHIDYGLISLPEGSMSTRKGQIITAREVLDRAQDRAEEIIAEKGRIDDAEAQSVATK
IALATIKYEMVAAKRERDTTFDIDESVALEGDTGPYVQYAATRGYSILDGADAAPEIDDLDPSVFNDTDVELLFELARYP
LVLERCEERYDAAPLAHYLLQLAHVFNSFYHKNAVLDAENARTERLLLTKATTQIFDNGLGLLGIDVLEEM

Specific function: Unknown

COG id: COG0018

COG function: function code J; Arginyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI197100773, Length=587, Percent_Identity=29.1311754684838, Blast_Score=232, Evalue=6e-61,
Organism=Homo sapiens, GI15149476, Length=573, Percent_Identity=28.0977312390925, Blast_Score=180, Evalue=3e-45,
Organism=Escherichia coli, GI1788184, Length=639, Percent_Identity=30.0469483568075, Blast_Score=222, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI71985061, Length=631, Percent_Identity=28.2091917591125, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71985068, Length=592, Percent_Identity=28.8851351351351, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17532153, Length=546, Percent_Identity=26.007326007326, Blast_Score=140, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6321883, Length=636, Percent_Identity=30.8176100628931, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6320548, Length=620, Percent_Identity=30, Blast_Score=255, Evalue=2e-68,
Organism=Drosophila melanogaster, GI18859963, Length=577, Percent_Identity=27.0363951473137, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI28571570, Length=526, Percent_Identity=26.9961977186312, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: 789 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYR_HALSA (Q9HHN2)

Other databases:

- EMBL:   AE004438
- RefSeq:   NP_395809.1
- ProteinModelPortal:   Q9HHN2
- SMR:   Q9HHN2
- GeneID:   1449192
- GenomeReviews:   AE004438_GR
- KEGG:   hal:VNG6312G
- NMPDR:   fig|64091.1.peg.2493
- HOGENOM:   HBG695395
- OMA:   AYKKWGN
- ProtClustDB:   CLSK2301553
- BioCyc:   HSP64091:VNG6312G-MONOMER
- BRENDA:   6.1.1.19
- GO:   GO:0005737
- HAMAP:   MF_00123
- InterPro:   IPR001412
- InterPro:   IPR001278
- InterPro:   IPR015945
- InterPro:   IPR005148
- InterPro:   IPR008909
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.30.1360.70
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11956
- PRINTS:   PR01038
- SMART:   SM00836
- TIGRFAMs:   TIGR00456

Pfam domain/function: PF03485 Arg_tRNA_synt_N; PF05746 DALR_1; PF00750 tRNA-synt_1d; SSF55190 Arg-tRNA-synth_Ic_N; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.19

Molecular weight: Translated: 70472; Mature: 70472

Theoretical pI: Translated: 3.95; Mature: 3.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MLYNLRQELLAGIRAATSDAGYDYEVDQSAIELEDITDEEKGEFSSPISFSIAAAAGAPP
CCCCHHHHHHHHHHHHCCCCCCCEECCCCCEEHHHCCCCCCCCCCCCCEEEEEECCCCCC
VDVAAAIADAHRSNGLPAEVEAVTVEGGHINYHADTTDLADATLSTILRDGSEYGTRTDA
HHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHCCHHCCCCCCC
DPDTILADVSSPNIAKPLHVGHLRNTILSDAVMNILEARGHDVTRDNHLGDWGVQFGNLM
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
HEYTEFGDEATLEDDAIEHLLDLYQQFEQRDSMLADLEDDETVTDQFADAVTEERDYHAD
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
SGKEWFTRLEQGDEDATALWERFRTVSIDRFKQTYDDLDVAFDVWNGESFYAQEGWNDVI
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCHHH
IEKAIENDVAMRGEGESVYIPVYPDDYENVGDPQAADVDASLDRARQMREANDDLEDADF
HHHHHHCCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
DPFYIVKSDGSTLYGTRDLATIEYRIEEYDADQSVYVVANEQNQYFQQLFVAARKMGYND
CCEEEEECCCCEEECCCCEEEEEEEEHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCC
IKLKHIDYGLISLPEGSMSTRKGQIITAREVLDRAQDRAEEIIAEKGRIDDAEAQSVATK
EEEEEECCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IALATIKYEMVAAKRERDTTFDIDESVALEGDTGPYVQYAATRGYSILDGADAAPEIDDL
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEHHCCCCEEECCCCCCCCCCCC
DPSVFNDTDVELLFELARYPLVLERCEERYDAAPLAHYLLQLAHVFNSFYHKNAVLDAEN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECCC
ARTERLLLTKATTQIFDNGLGLLGIDVLEEM
CCCCEEEEHHHHHHHHHCCCCEEHHHHHHCC
>Mature Secondary Structure
MLYNLRQELLAGIRAATSDAGYDYEVDQSAIELEDITDEEKGEFSSPISFSIAAAAGAPP
CCCCHHHHHHHHHHHHCCCCCCCEECCCCCEEHHHCCCCCCCCCCCCCEEEEEECCCCCC
VDVAAAIADAHRSNGLPAEVEAVTVEGGHINYHADTTDLADATLSTILRDGSEYGTRTDA
HHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHHCCHHCCCCCCC
DPDTILADVSSPNIAKPLHVGHLRNTILSDAVMNILEARGHDVTRDNHLGDWGVQFGNLM
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
HEYTEFGDEATLEDDAIEHLLDLYQQFEQRDSMLADLEDDETVTDQFADAVTEERDYHAD
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
SGKEWFTRLEQGDEDATALWERFRTVSIDRFKQTYDDLDVAFDVWNGESFYAQEGWNDVI
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCHHH
IEKAIENDVAMRGEGESVYIPVYPDDYENVGDPQAADVDASLDRARQMREANDDLEDADF
HHHHHHCCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
DPFYIVKSDGSTLYGTRDLATIEYRIEEYDADQSVYVVANEQNQYFQQLFVAARKMGYND
CCEEEEECCCCEEECCCCEEEEEEEEHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCC
IKLKHIDYGLISLPEGSMSTRKGQIITAREVLDRAQDRAEEIIAEKGRIDDAEAQSVATK
EEEEEECCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IALATIKYEMVAAKRERDTTFDIDESVALEGDTGPYVQYAATRGYSILDGADAAPEIDDL
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEHHCCCCEEECCCCCCCCCCCC
DPSVFNDTDVELLFELARYPLVLERCEERYDAAPLAHYLLQLAHVFNSFYHKNAVLDAEN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEECCC
ARTERLLLTKATTQIFDNGLGLLGIDVLEEM
CCCCEEEEHHHHHHHHHCCCCEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11016950