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The map label for this gene is pyrB

Identifier: 16120219

GI number: 16120219

Start: 239301

End: 240326

Strand: Direct

Name: pyrB

Synonym: VNG6309G

Alternate gene names: 16120219

Gene position: 239301-240326 (Clockwise)

Preceding gene: 16120214

Following gene: 16120220

Centisome position: 65.49

GC content: 61.79

Gene sequence:

>1026_bases
ATGCTGTCGACGGATGCCGTTGAAACGTTCGATCAGTTAGTTGTTCATGATAGAAAGGAAGATTCAAGTCACCTGGACCG
GAGACACAGAGTGATGGATCTCGACCACATCATCACGAGCAAACAACTCTCCCGGGCGGACATCGAGACCGTGCTCGACC
GCGCGGCAGAGATCGACGCCGACCCGTCGGCGTTCGCCGACCGGTACGAAAACACCGTGCTCGGACTCCTCTTTTTCGAG
CCGAGCACCCGCACCAAGATGAGCTTCGACACCGCGATGAAACGCCTGGGCGGCGACATCGTCGATATGGGGTCCGTAGA
CTCCTCAAGCGTCAAGAAGGGGGAGACACTCGCAGACACGGTTCGCGTCATCGAAGGGTACGCGGACGCGTTGGTGCTGC
GCCACCCCAACCAGGGAGCAGCCCAGATGGCGAGCGAGTTCGTCGACGTTCCGCTTTTGAACGCCGGCGACGGCGCCGGC
CATCACCCGACACAGACCCTCCTGGACCTCTACACCATCCGCGAGAACGCCGGCCTCGAAGACCTCACGATCGGCATCAT
GGGGGATCTCAAGTACGGGCGGACGGTCCACTCGCTGTCCCGCGCGCTCACGAACTTCGACGTCGACCAACACTTCATCA
GTCCCGAGAGCCTCCAGCTCCCCCGAGACGTCGTCTACGACCTCGAACAGGAGTCCGGCACTCGTGTCCGCGAACACGAC
TCCTTCGAGGACGTGCTCCCGGAGCTGGACATCCTTTACGTGACTCGGATCCAGCGCGAGCGGTTCCCCGACGAAAACGA
GTACCAGAAGGTTGCCGGAGAGTACCAACTCACCGCCACCCACCTCGAAGCCGCCAAAGACGACCTGACCATCATGCATC
CGCTCCCGCGAGTCGACGAGATCGCACCCGAAATCGACGACACCGACCACGCGGCCTACTTCGAGCAGGCACACAACGGG
GTGCCGGTTCGCATGGCACTGCTGGATCTGGTGCTGTCCGCCGATGGGGGTGTCGACGATGAGTAG

Upstream 100 bases:

>100_bases
CGGGAACTCAACACGCGTTTGAACTCCCACGAATAAAGCTCCTGTTTGGATATTCCGGAAAAACGCGCCATAGCTAGCGA
GACCACAGCGGGATCGGCGG

Downstream 100 bases:

>100_bases
CGACCAACACCTCCAGGTCAGCAAAATCCAGGCCGGAACCGTCATCGACCACATCCCGGCCGGGCAGGCGCTGCAAGTGC
TTCAGATCCTCGGGACCAAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MLSTDAVETFDQLVVHDRKEDSSHLDRRHRVMDLDHIITSKQLSRADIETVLDRAAEIDADPSAFADRYENTVLGLLFFE
PSTRTKMSFDTAMKRLGGDIVDMGSVDSSSVKKGETLADTVRVIEGYADALVLRHPNQGAAQMASEFVDVPLLNAGDGAG
HHPTQTLLDLYTIRENAGLEDLTIGIMGDLKYGRTVHSLSRALTNFDVDQHFISPESLQLPRDVVYDLEQESGTRVREHD
SFEDVLPELDILYVTRIQRERFPDENEYQKVAGEYQLTATHLEAAKDDLTIMHPLPRVDEIAPEIDDTDHAAYFEQAHNG
VPVRMALLDLVLSADGGVDDE

Sequences:

>Translated_341_residues
MLSTDAVETFDQLVVHDRKEDSSHLDRRHRVMDLDHIITSKQLSRADIETVLDRAAEIDADPSAFADRYENTVLGLLFFE
PSTRTKMSFDTAMKRLGGDIVDMGSVDSSSVKKGETLADTVRVIEGYADALVLRHPNQGAAQMASEFVDVPLLNAGDGAG
HHPTQTLLDLYTIRENAGLEDLTIGIMGDLKYGRTVHSLSRALTNFDVDQHFISPESLQLPRDVVYDLEQESGTRVREHD
SFEDVLPELDILYVTRIQRERFPDENEYQKVAGEYQLTATHLEAAKDDLTIMHPLPRVDEIAPEIDDTDHAAYFEQAHNG
VPVRMALLDLVLSADGGVDDE
>Mature_341_residues
MLSTDAVETFDQLVVHDRKEDSSHLDRRHRVMDLDHIITSKQLSRADIETVLDRAAEIDADPSAFADRYENTVLGLLFFE
PSTRTKMSFDTAMKRLGGDIVDMGSVDSSSVKKGETLADTVRVIEGYADALVLRHPNQGAAQMASEFVDVPLLNAGDGAG
HHPTQTLLDLYTIRENAGLEDLTIGIMGDLKYGRTVHSLSRALTNFDVDQHFISPESLQLPRDVVYDLEQESGTRVREHD
SFEDVLPELDILYVTRIQRERFPDENEYQKVAGEYQLTATHLEAAKDDLTIMHPLPRVDEIAPEIDDTDHAAYFEQAHNG
VPVRMALLDLVLSADGGVDDE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=267, Percent_Identity=47.565543071161, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=25.2427184466019, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=50.328947368421, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1790703, Length=273, Percent_Identity=23.4432234432234, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=328, Percent_Identity=42.0731707317073, Blast_Score=227, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=303, Percent_Identity=42.5742574257426, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322373, Length=332, Percent_Identity=22.289156626506, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24642586, Length=263, Percent_Identity=49.0494296577947, Blast_Score=259, Evalue=1e-69,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 38072; Mature: 38072

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTDAVETFDQLVVHDRKEDSSHLDRRHRVMDLDHIITSKQLSRADIETVLDRAAEIDA
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DPSAFADRYENTVLGLLFFEPSTRTKMSFDTAMKRLGGDIVDMGSVDSSSVKKGETLADT
CHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHCCHHHHHH
VRVIEGYADALVLRHPNQGAAQMASEFVDVPLLNAGDGAGHHPTQTLLDLYTIRENAGLE
HHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
DLTIGIMGDLKYGRTVHSLSRALTNFDVDQHFISPESLQLPRDVVYDLEQESGTRVREHD
CEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCHHHCCCHHHHHHHHHCCCCCCHHCC
SFEDVLPELDILYVTRIQRERFPDENEYQKVAGEYQLTATHLEAAKDDLTIMHPLPRVDE
CHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEHHHHHHCCCCEEEECCCCCHHH
IAPEIDDTDHAAYFEQAHNGVPVRMALLDLVLSADGGVDDE
HCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLSTDAVETFDQLVVHDRKEDSSHLDRRHRVMDLDHIITSKQLSRADIETVLDRAAEIDA
CCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DPSAFADRYENTVLGLLFFEPSTRTKMSFDTAMKRLGGDIVDMGSVDSSSVKKGETLADT
CHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHCCHHHHHH
VRVIEGYADALVLRHPNQGAAQMASEFVDVPLLNAGDGAGHHPTQTLLDLYTIRENAGLE
HHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
DLTIGIMGDLKYGRTVHSLSRALTNFDVDQHFISPESLQLPRDVVYDLEQESGTRVREHD
CEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHCCCHHHCCCHHHHHHHHHCCCCCCHHCC
SFEDVLPELDILYVTRIQRERFPDENEYQKVAGEYQLTATHLEAAKDDLTIMHPLPRVDE
CHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEHHHHHHCCCCEEEECCCCCHHH
IAPEIDDTDHAAYFEQAHNGVPVRMALLDLVLSADGGVDDE
HCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11016950 [H]