BacMap

Definition	Chlamydophila pneumoniae J138, complete genome.
Accession	NC_002491
Length	1,226,565

Click here to switch to the map view.

The map label for this gene is lepA

Identifier: 15835892

GI number: 15835892

Start: 401721

End: 403529

Strand: Direct

Name: lepA

Synonym: CPj0359

Alternate gene names: 15835892

Gene position: 401721-403529 (Clockwise)

Preceding gene: 15835887

Following gene: 15835897

Centisome position: 32.75

GC content: 40.85

Gene sequence:

>1809_bases
TTGAAAGAATATAAGATAGAGAACATTCGCAATTTTTCAATCATAGCGCATATTGATCACGGGAAGTCTACAATTGCTGA
TCGCCTTTTAGAAAGTACGAGCACAGTAGAAGAACGGGAGATGCGTGAGCAGCTCTTAGATTCCATGGATCTTGAAAGAG
AGCGTGGCATTACAATTAAAGCTCATCCTGTCACCATGACGTATCTATATGAAGGAGAGGTGTATCAACTGAACCTGATT
GATACCCCTGGTCACGTGGACTTTTCGTATGAAGTCTCTCGATCTCTATCTGCATGTGAGGGCGCCTTACTTATTGTAGA
TGCCGCCCAGGGGGTGCAGGCACAAAGTCTTGCTAATGTCTACCTGGCCCTTGAAAGAGATTTAGAGATCATTCCTGTAT
TAAACAAGATTGATCTACCTGCCGCTGATCCCGTGAGAATTGCTCAACAGATTGAAGATTATATAGGCCTAGACACTACG
AACATTATTGCCTGTTCTGCAAAAACAGGTCAGGGGATCCCTGCAATCCTGAAAGCAATTATCGATCTTGTTCCTCCTCC
AAAAGCACCTGCAGAAACAGAGCTTAAAGCTTTAGTCTTTGATTCTCATTATGACCCTTACGTTGGCATTATGGTCTACG
TACGCATTATTAGCGGGGAATTAAAAAAAGGAGACCGCATTACTTTTATGGCGGCTAAAGGCTCCTCGTTTGAAGTCTTA
GGTATAGGGGCCTTTCTCCCTAAAGCAACATTTATAGAAGGTTCCTTACGCCCTGGTCAGGTGGGTTTTTTTATTGCCAA
TCTCAAAAAAGTGAAGGATGTGAAGATCGGCGATACAGTCACGAAAACAAAACATCCTGCAAAAACTCCTTTGGAAGGCT
TCAAAGAGATCAATCCGGTAGTTTTTGCTGGAATTTATCCTATAGATTCTTCTGATTTTGATACTTTGAAAGATGCTTTA
GGAAGACTACAGCTCAATGATTCTGCTTTAACTATAGAACAAGAAAGCAGTCACTCTTTAGGCTTTGGTTTTCGTTGTGG
CTTCTTAGGACTTCTTCATCTTGAGATTATCTTTGAAAGAATCATTCGAGAATTTGACTTAGATATTATTGCAACGGCTC
CAAGTGTCATCTATAAAGTCGTCTTAAAAAACGGGAAAGTTCTAGATATTGATAACCCCTCAGGATATCCGGATCCTGCG
ATCATCGAGCATGTGGAAGAGCCTTGGGTTCATGTGAATATTATCACCCCTCAAGAATATCTGAGCAACATTATGAACCT
CTGTTTAGATAAACGTGGGATCTGCGTAAAAACAGAAATGCTAGATCAGCACCGTCTAGTTCTTGCTTACGAACTCCCTT
TAAATGAGATTGTCTCGGATTTCAATGACAAGCTGAAGTCAGTAACTAAAGGTTATGGATCCTTTGACTACCGTCTTGGG
GATTACCGTAAGGGATCGATCATCAAATTAGAGGTTCTTATTAACGAGGAGCCCATAGATGCTTTTTCTTGTTTAGTCCA
TAGAGATAAAGCAGAATCTCGTGGAAGAAGTATCTGCGAAAAGCTTGTGGACGTGATTCCACAACAACTCTTCAAGATTC
CCATCCAAGCTGCCATTAACAAAAAAGTCATTGCCAGAGAAACGATTCGTGCGCTTTCTAAGAACGTGACCGCAAAGTGT
TATGGCGGAGATATTACTAGGAAACGCAAGCTGTGGGAAAAGCAAAAGAAAGGAAAAAAACGTATGAAGGAATTTGGAAA
AGTTTCCATTCCCAATACAGCTTTCATTGAAGTTCTAAAATTAGATTAA

Upstream 100 bases:

>100_bases
GTCCGCAAAAGTATTTGAGAAGCTGGTCTTTGTCTGAAGGAGCCTTCTCAATGCAGCTTTATGAAACCAGGAATAAACCT
AGATAACACTACAGTACATT

Downstream 100 bases:

>100_bases
CATTGACGCTTAAAATCAGCACACTGCTTACAATTGAAAATTCGGTAGTGGTGAACTAAATCTCGAGCTACCTAGGGTCT
TCTCGAGATTTTTTATTTTT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MKEYKIENIRNFSIIAHIDHGKSTIADRLLESTSTVEEREMREQLLDSMDLERERGITIKAHPVTMTYLYEGEVYQLNLI
DTPGHVDFSYEVSRSLSACEGALLIVDAAQGVQAQSLANVYLALERDLEIIPVLNKIDLPAADPVRIAQQIEDYIGLDTT
NIIACSAKTGQGIPAILKAIIDLVPPPKAPAETELKALVFDSHYDPYVGIMVYVRIISGELKKGDRITFMAAKGSSFEVL
GIGAFLPKATFIEGSLRPGQVGFFIANLKKVKDVKIGDTVTKTKHPAKTPLEGFKEINPVVFAGIYPIDSSDFDTLKDAL
GRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFERIIREFDLDIIATAPSVIYKVVLKNGKVLDIDNPSGYPDPA
IIEHVEEPWVHVNIITPQEYLSNIMNLCLDKRGICVKTEMLDQHRLVLAYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
DYRKGSIIKLEVLINEEPIDAFSCLVHRDKAESRGRSICEKLVDVIPQQLFKIPIQAAINKKVIARETIRALSKNVTAKC
YGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLKLD

Sequences:

>Translated_602_residues
MKEYKIENIRNFSIIAHIDHGKSTIADRLLESTSTVEEREMREQLLDSMDLERERGITIKAHPVTMTYLYEGEVYQLNLI
DTPGHVDFSYEVSRSLSACEGALLIVDAAQGVQAQSLANVYLALERDLEIIPVLNKIDLPAADPVRIAQQIEDYIGLDTT
NIIACSAKTGQGIPAILKAIIDLVPPPKAPAETELKALVFDSHYDPYVGIMVYVRIISGELKKGDRITFMAAKGSSFEVL
GIGAFLPKATFIEGSLRPGQVGFFIANLKKVKDVKIGDTVTKTKHPAKTPLEGFKEINPVVFAGIYPIDSSDFDTLKDAL
GRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFERIIREFDLDIIATAPSVIYKVVLKNGKVLDIDNPSGYPDPA
IIEHVEEPWVHVNIITPQEYLSNIMNLCLDKRGICVKTEMLDQHRLVLAYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
DYRKGSIIKLEVLINEEPIDAFSCLVHRDKAESRGRSICEKLVDVIPQQLFKIPIQAAINKKVIARETIRALSKNVTAKC
YGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLKLD
>Mature_602_residues
MKEYKIENIRNFSIIAHIDHGKSTIADRLLESTSTVEEREMREQLLDSMDLERERGITIKAHPVTMTYLYEGEVYQLNLI
DTPGHVDFSYEVSRSLSACEGALLIVDAAQGVQAQSLANVYLALERDLEIIPVLNKIDLPAADPVRIAQQIEDYIGLDTT
NIIACSAKTGQGIPAILKAIIDLVPPPKAPAETELKALVFDSHYDPYVGIMVYVRIISGELKKGDRITFMAAKGSSFEVL
GIGAFLPKATFIEGSLRPGQVGFFIANLKKVKDVKIGDTVTKTKHPAKTPLEGFKEINPVVFAGIYPIDSSDFDTLKDAL
GRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFERIIREFDLDIIATAPSVIYKVVLKNGKVLDIDNPSGYPDPA
IIEHVEEPWVHVNIITPQEYLSNIMNLCLDKRGICVKTEMLDQHRLVLAYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
DYRKGSIIKLEVLINEEPIDAFSCLVHRDKAESRGRSICEKLVDVIPQQLFKIPIQAAINKKVIARETIRALSKNVTAKC
YGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLKLD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=608, Percent_Identity=48.6842105263158, Blast_Score=600, Evalue=1e-171,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=42.4242424242424, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI18390331, Length=204, Percent_Identity=34.3137254901961, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI25306283, Length=140, Percent_Identity=42.1428571428571, Blast_Score=98, Evalue=3e-20,
Organism=Homo sapiens, GI19923640, Length=140, Percent_Identity=42.1428571428571, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI25306287, Length=140, Percent_Identity=42.1428571428571, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI4503483, Length=147, Percent_Identity=36.734693877551, Blast_Score=97, Evalue=7e-20,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=41.2844036697248, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=41.2844036697248, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=41.2844036697248, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI217272894, Length=136, Percent_Identity=38.9705882352941, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI217272892, Length=136, Percent_Identity=38.9705882352941, Blast_Score=90, Evalue=8e-18,
Organism=Homo sapiens, GI53729339, Length=225, Percent_Identity=26.2222222222222, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI53729337, Length=225, Percent_Identity=26.2222222222222, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788922, Length=598, Percent_Identity=51.505016722408, Blast_Score=626, Evalue=1e-180,
Organism=Escherichia coli, GI48994988, Length=515, Percent_Identity=27.1844660194175, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=35.8490566037736, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790835, Length=167, Percent_Identity=32.3353293413174, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1789559, Length=220, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=620, Percent_Identity=38.7096774193548, Blast_Score=446, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17556745, Length=459, Percent_Identity=25.7080610021786, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=148, Percent_Identity=39.1891891891892, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=37.593984962406, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=37.593984962406, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=34.6153846153846, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=224, Percent_Identity=24.1071428571429, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=42.7152317880795, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6323098, Length=186, Percent_Identity=35.4838709677419, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=37.4149659863946, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=37.4149659863946, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=114, Percent_Identity=36.8421052631579, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=37.5886524822695, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78706572, Length=603, Percent_Identity=42.4543946932007, Blast_Score=521, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24582462, Length=185, Percent_Identity=37.2972972972973, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=41.304347826087, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=36.4238410596026, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=36.4238410596026, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585709, Length=151, Percent_Identity=36.4238410596026, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=165, Percent_Identity=35.7575757575758, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI221458488, Length=150, Percent_Identity=36.6666666666667, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI28572034, Length=225, Percent_Identity=29.3333333333333, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67287; Mature: 67287

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEYKIENIRNFSIIAHIDHGKSTIADRLLESTSTVEEREMREQLLDSMDLERERGITIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHCCCEEE
AHPVTMTYLYEGEVYQLNLIDTPGHVDFSYEVSRSLSACEGALLIVDAAQGVQAQSLANV
ECCEEEEEEECCCEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
YLALERDLEIIPVLNKIDLPAADPVRIAQQIEDYIGLDTTNIIACSAKTGQGIPAILKAI
HEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
IDLVPPPKAPAETELKALVFDSHYDPYVGIMVYVRIISGELKKGDRITFMAAKGSSFEVL
HHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEE
GIGAFLPKATFIEGSLRPGQVGFFIANLKKVKDVKIGDTVTKTKHPAKTPLEGFKEINPV
EEHHHCCCCEEEECCCCCCCEEEEEECHHHHCCEECCCCCCCCCCCCCCCHHHHHHCCCE
VFAGIYPIDSSDFDTLKDALGRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFER
EEEEEECCCCCCHHHHHHHHCCEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
IIREFDLDIIATAPSVIYKVVLKNGKVLDIDNPSGYPDPAIIEHVEEPWVHVNIITPQEY
HHHHHCCEEEECCHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCCCEEEEEEECHHHH
LSNIMNLCLDKRGICVKTEMLDQHRLVLAYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
HHHHHHHHHCCCCCEEEHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEECCCC
DYRKGSIIKLEVLINEEPIDAFSCLVHRDKAESRGRSICEKLVDVIPQQLFKIPIQAAIN
CCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
KKVIARETIRALSKNVTAKCYGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLK
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
LD
CC
>Mature Secondary Structure
MKEYKIENIRNFSIIAHIDHGKSTIADRLLESTSTVEEREMREQLLDSMDLERERGITIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHCCCEEE
AHPVTMTYLYEGEVYQLNLIDTPGHVDFSYEVSRSLSACEGALLIVDAAQGVQAQSLANV
ECCEEEEEEECCCEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHH
YLALERDLEIIPVLNKIDLPAADPVRIAQQIEDYIGLDTTNIIACSAKTGQGIPAILKAI
HEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHH
IDLVPPPKAPAETELKALVFDSHYDPYVGIMVYVRIISGELKKGDRITFMAAKGSSFEVL
HHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEE
GIGAFLPKATFIEGSLRPGQVGFFIANLKKVKDVKIGDTVTKTKHPAKTPLEGFKEINPV
EEHHHCCCCEEEECCCCCCCEEEEEECHHHHCCEECCCCCCCCCCCCCCCHHHHHHCCCE
VFAGIYPIDSSDFDTLKDALGRLQLNDSALTIEQESSHSLGFGFRCGFLGLLHLEIIFER
EEEEEECCCCCCHHHHHHHHCCEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
IIREFDLDIIATAPSVIYKVVLKNGKVLDIDNPSGYPDPAIIEHVEEPWVHVNIITPQEY
HHHHHCCEEEECCHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCCCEEEEEEECHHHH
LSNIMNLCLDKRGICVKTEMLDQHRLVLAYELPLNEIVSDFNDKLKSVTKGYGSFDYRLG
HHHHHHHHHCCCCCEEEHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEECCCC
DYRKGSIIKLEVLINEEPIDAFSCLVHRDKAESRGRSICEKLVDVIPQQLFKIPIQAAIN
CCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
KKVIARETIRALSKNVTAKCYGGDITRKRKLWEKQKKGKKRMKEFGKVSIPNTAFIEVLK
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
LD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA