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The map label for this gene is capI [H]

Identifier: 15594789

GI number: 15594789

Start: 462819

End: 463886

Strand: Direct

Name: capI [H]

Synonym: BB0444

Alternate gene names: 15594789

Gene position: 462819-463886 (Clockwise)

Preceding gene: 15594788

Following gene: 15594790

Centisome position: 50.82

GC content: 31.27

Gene sequence:

>1068_bases
ATGAAGATTTTTTTAACCGGAATTGCAGGGTTTATTGGATTTCATGTAGCTAAAAAGCTTGTGGAAAAAGGGCATGAAGT
TTTGGGAATAGACGTATTAAATGATTATTATGAACTCAAATTTAAGCATGAAAGGTTAGAAGCTTTAGGGTTTTGTTCTA
AGGATGTTAAAACGCATAAGATTATTAAGAGTGAAAAATATAATAATTTATCTTTTGCTTATCTTGATATACTAAATAAG
GATAAACTGCTAGAACTTTTTAAAGATCATAAGTTTACACATGTTTGCCATTTGGCTGCTCAAGCGGGCATTAGAGATAG
TCTTGAAAATCCTGATAGCTATGTTTCAATAAATATTGTTGGGTTTTTTAATGTTTTGGATGTATGTAGAGTTTATAAAG
AAAACATCGAGCATTTTGTTTATGCATCAACGTCATCGGTTTATGGCATAAATGAAAATATGCCGTCTAGTGAAGATTCT
ATTACGGATCACCCTTTAAATTTATATGCAGCTAGTAAAAAATCTAATGAGATGATGGCGCATGCTTATAGTGCATCTTT
TAATATTCCCACAACAGGGCTTAGATTTTTCACAGTTTATGGGACTTATGGAAGACCCGATATGGCTTTATATTTATTTT
CAGATGGAATTAAAAATGGCAAGGCTATTAATATTTTTAATAATGGGAATATGGCTAGAGATTTTACATATGTGGGTGAC
ATTGCAGACGGTGTTTACAAAGTTTTGAAAAATCCGGCTAAGAGTGATTGTAATTTTGACGTTAAAAATCCAAATTCATC
AACTTCTTCTTTTCCTTACAGAATATATAATATAGGAACTGGACACGCAACTAAACTGCTAGATTTTATTAGCGAGCTTG
AAGCAAATTTTGATAAAAAGGCCTTAAAAAATTATATGCCTATGCAAAAAGCAGATGTTGTAGAAAGTTGTTGTGATATT
TTAAAGCTTAAAAATGATGTTGGGTATGAGGCTAAAGTTTCTATTAAAGAAGGAATAAAAGAATTTTCACAGTGGTATAA
AATGCTAGAGTCTACCAAGAAGACTTGA

Upstream 100 bases:

>100_bases
TGGTATCAACAATTCTCGTGGTTATCAGAAAAGAGATATTGGTTTTAAAAAATAGATTAATGGGCTTGCTTTGTATTGAA
TTTTAAAGAGGAATAATAGT

Downstream 100 bases:

>100_bases
TTTTAAACTTTTTTATGCAAAATAATAAATTTTTAATAAATTTTATGTTTGGTTTTGATTTAATAATTATTTATTTTTTG
ATTTAAATTCAATTCAACTT

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIFLTGIAGFIGFHVAKKLVEKGHEVLGIDVLNDYYELKFKHERLEALGFCSKDVKTHKIIKSEKYNNLSFAYLDILNK
DKLLELFKDHKFTHVCHLAAQAGIRDSLENPDSYVSINIVGFFNVLDVCRVYKENIEHFVYASTSSVYGINENMPSSEDS
ITDHPLNLYAASKKSNEMMAHAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNGKAINIFNNGNMARDFTYVGD
IADGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFISELEANFDKKALKNYMPMQKADVVESCCDI
LKLKNDVGYEAKVSIKEGIKEFSQWYKMLESTKKT

Sequences:

>Translated_355_residues
MKIFLTGIAGFIGFHVAKKLVEKGHEVLGIDVLNDYYELKFKHERLEALGFCSKDVKTHKIIKSEKYNNLSFAYLDILNK
DKLLELFKDHKFTHVCHLAAQAGIRDSLENPDSYVSINIVGFFNVLDVCRVYKENIEHFVYASTSSVYGINENMPSSEDS
ITDHPLNLYAASKKSNEMMAHAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNGKAINIFNNGNMARDFTYVGD
IADGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFISELEANFDKKALKNYMPMQKADVVESCCDI
LKLKNDVGYEAKVSIKEGIKEFSQWYKMLESTKKT
>Mature_355_residues
MKIFLTGIAGFIGFHVAKKLVEKGHEVLGIDVLNDYYELKFKHERLEALGFCSKDVKTHKIIKSEKYNNLSFAYLDILNK
DKLLELFKDHKFTHVCHLAAQAGIRDSLENPDSYVSINIVGFFNVLDVCRVYKENIEHFVYASTSSVYGINENMPSSEDS
ITDHPLNLYAASKKSNEMMAHAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNGKAINIFNNGNMARDFTYVGD
IADGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFISELEANFDKKALKNYMPMQKADVVESCCDI
LKLKNDVGYEAKVSIKEGIKEFSQWYKMLESTKKT

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=364, Percent_Identity=23.9010989010989, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI56118217, Length=364, Percent_Identity=23.9010989010989, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI189083684, Length=364, Percent_Identity=23.9010989010989, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI7657641, Length=353, Percent_Identity=25.7790368271955, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI42516563, Length=361, Percent_Identity=24.9307479224377, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI48994969, Length=376, Percent_Identity=23.936170212766, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788353, Length=381, Percent_Identity=26.7716535433071, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786974, Length=363, Percent_Identity=21.4876033057851, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71982038, Length=365, Percent_Identity=24.1095890410959, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=374, Percent_Identity=25.1336898395722, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=357, Percent_Identity=24.0896358543417, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=275, Percent_Identity=24, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=366, Percent_Identity=24.8633879781421, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=366, Percent_Identity=24.8633879781421, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=366, Percent_Identity=24.8633879781421, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=366, Percent_Identity=24.8633879781421, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=365, Percent_Identity=24.3835616438356, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=360, Percent_Identity=23.0555555555556, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=370, Percent_Identity=22.4324324324324, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=23.2686980609418, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356223, Length=358, Percent_Identity=23.7430167597765, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 40183; Mature: 40183

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFLTGIAGFIGFHVAKKLVEKGHEVLGIDVLNDYYELKFKHERLEALGFCSKDVKTHK
CEEEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IIKSEKYNNLSFAYLDILNKDKLLELFKDHKFTHVCHLAAQAGIRDSLENPDSYVSINIV
HHHHCCCCCCEEEEEEHHCHHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCEEEEEEE
GFFNVLDVCRVYKENIEHFVYASTSSVYGINENMPSSEDSITDHPLNLYAASKKSNEMMA
EHHHHHHHHHHHHHCHHHEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHH
HAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNGKAINIFNNGNMARDFTYVGD
HHHHCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEECCCCCEEEEEHHH
IADGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFISELEANFDKK
HHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHH
ALKNYMPMQKADVVESCCDILKLKNDVGYEAKVSIKEGIKEFSQWYKMLESTKKT
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIFLTGIAGFIGFHVAKKLVEKGHEVLGIDVLNDYYELKFKHERLEALGFCSKDVKTHK
CEEEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IIKSEKYNNLSFAYLDILNKDKLLELFKDHKFTHVCHLAAQAGIRDSLENPDSYVSINIV
HHHHCCCCCCEEEEEEHHCHHHHHHHHHHCCHHHHHHHHHHHCCHHHCCCCCCEEEEEEE
GFFNVLDVCRVYKENIEHFVYASTSSVYGINENMPSSEDSITDHPLNLYAASKKSNEMMA
EHHHHHHHHHHHHHCHHHEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCHHHH
HAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNGKAINIFNNGNMARDFTYVGD
HHHHCCCCCCCCCEEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEECCCCCEEEEEHHH
IADGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFISELEANFDKK
HHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHH
ALKNYMPMQKADVVESCCDILKLKNDVGYEAKVSIKEGIKEFSQWYKMLESTKKT
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]