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The map label for this gene is fba

Identifier: 15594790

GI number: 15594790

Start: 464053

End: 465132

Strand: Direct

Name: fba

Synonym: BB0445

Alternate gene names: 15594790

Gene position: 464053-465132 (Clockwise)

Preceding gene: 15594789

Following gene: 15594798

Centisome position: 50.95

GC content: 32.78

Gene sequence:

>1080_bases
ATGGGTGTTTTAGATAAGATTAAACCAGGTGTAGTTTATGGGAAGGAACTACATTTTTTATATGAAATATGTAAAAAGGA
AGGATTTGCTATTCCTTCTATTAATTGTATAGGAACAAATTCTATTAATGCAGTTTTGGAGGCAGCAAAAGAAATTAATT
CTCCTATTATGATACAATTTTCTAATAGTGGATCTGCTTTTATTTCTGGAAAAGGATTAAAGATGGAAAAACCACAAGGA
GTTTCAATAGTTGGAGCTATTTCTGGTGCTATGCATGTTCATTTAATGGCAGAGCATTATGGTGTTCCTGTTGTTCTTCA
TACTGATCACTGTGCTAAAAATTTGCTTCCTTGGGTTGAAGGCCTTTTAGAATATGGAGAGAAATACTATAGTCAGCACA
AAAAACCATTATTTTCTTCACATATGTTAGATTTATCAGAAGAACCTATTAAAGAAAATATTGAAATTTCTAAAAAATTC
TTAGAAAGAATGGCAAAAATTGAAATGTTTTTGGAAATAGAGCTTGGAATTACGGGTGGGGAAGAGGATGGAGTTGACAA
TTCAGATAGAGCTTTGCATGAACTATTTTCTACTCCTGAGGATATTTATTATGGATATTCAGAACTTTTAAAAGTTAGCC
CAAATTTTCAGATTGCAGCAGCTTTTGGAAATGTTCATGGGGTATATAAACCGGGGAATGTTAAGCTTACTCCAAAAGTT
TTAAAAGATGGTCAAGATTATGTCATATCAAAAACAGGAGTAAATATGGCTAAGCCAGTTTCTTATGTTTTTCATGGAGG
GTCTGGATCTACAATTGATGAGATTAATGAGGCGCTTTCTTATGGCGTTGTAAAGATGAATATTGACACAGATACACAGT
GGGCTGCCTGGGAGGGTGTTTTAAATTATTACAAAAAAAATGAAAGTCGTTTGCAAGGTCAATTAGGAGATGGCAAGGAT
ATTGATATTCCAAATAAGAAATTTTATGATCCAAGGGTTTGGTTAAGAGAAGCTGAAGTTTCTATGAAAGACCGTGTGAA
GATTGCATGCAAAAATCTTAATAATATTAATAGAAATTAA

Upstream 100 bases:

>100_bases
TTATTTATTTTTTGATTTAAATTCAATTCAACTTCTTAGGTGTATAATTTATTATAGGCATTTGATAAAAGAAAAAATAA
AATGGAAAGGAGTGATATAA

Downstream 100 bases:

>100_bases
ATAAAAATTCACTAATTTTTATTTGCAAGCTGCTTTTTGAGCGGCTTTTTTATTATTCTAATTATATTTTAGATATTTAT
AAAATTATTGATTGTTTGAT

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MGVLDKIKPGVVYGKELHFLYEICKKEGFAIPSINCIGTNSINAVLEAAKEINSPIMIQFSNSGSAFISGKGLKMEKPQG
VSIVGAISGAMHVHLMAEHYGVPVVLHTDHCAKNLLPWVEGLLEYGEKYYSQHKKPLFSSHMLDLSEEPIKENIEISKKF
LERMAKIEMFLEIELGITGGEEDGVDNSDRALHELFSTPEDIYYGYSELLKVSPNFQIAAAFGNVHGVYKPGNVKLTPKV
LKDGQDYVISKTGVNMAKPVSYVFHGGSGSTIDEINEALSYGVVKMNIDTDTQWAAWEGVLNYYKKNESRLQGQLGDGKD
IDIPNKKFYDPRVWLREAEVSMKDRVKIACKNLNNINRN

Sequences:

>Translated_359_residues
MGVLDKIKPGVVYGKELHFLYEICKKEGFAIPSINCIGTNSINAVLEAAKEINSPIMIQFSNSGSAFISGKGLKMEKPQG
VSIVGAISGAMHVHLMAEHYGVPVVLHTDHCAKNLLPWVEGLLEYGEKYYSQHKKPLFSSHMLDLSEEPIKENIEISKKF
LERMAKIEMFLEIELGITGGEEDGVDNSDRALHELFSTPEDIYYGYSELLKVSPNFQIAAAFGNVHGVYKPGNVKLTPKV
LKDGQDYVISKTGVNMAKPVSYVFHGGSGSTIDEINEALSYGVVKMNIDTDTQWAAWEGVLNYYKKNESRLQGQLGDGKD
IDIPNKKFYDPRVWLREAEVSMKDRVKIACKNLNNINRN
>Mature_358_residues
GVLDKIKPGVVYGKELHFLYEICKKEGFAIPSINCIGTNSINAVLEAAKEINSPIMIQFSNSGSAFISGKGLKMEKPQGV
SIVGAISGAMHVHLMAEHYGVPVVLHTDHCAKNLLPWVEGLLEYGEKYYSQHKKPLFSSHMLDLSEEPIKENIEISKKFL
ERMAKIEMFLEIELGITGGEEDGVDNSDRALHELFSTPEDIYYGYSELLKVSPNFQIAAAFGNVHGVYKPGNVKLTPKVL
KDGQDYVISKTGVNMAKPVSYVFHGGSGSTIDEINEALSYGVVKMNIDTDTQWAAWEGVLNYYKKNESRLQGQLGDGKDI
DIPNKKFYDPRVWLREAEVSMKDRVKIACKNLNNINRN

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family

Homologues:

Organism=Escherichia coli, GI1789293, Length=355, Percent_Identity=61.6901408450704, Blast_Score=476, Evalue=1e-136,
Organism=Escherichia coli, GI87082041, Length=327, Percent_Identity=22.3241590214067, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788072, Length=271, Percent_Identity=26.9372693726937, Blast_Score=65, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322790, Length=359, Percent_Identity=45.9610027855153, Blast_Score=336, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ALF_BORBU (O51401)

Other databases:

- EMBL:   AE000783
- PIR:   D70155
- RefSeq:   NP_212579.1
- ProteinModelPortal:   O51401
- SMR:   O51401
- EnsemblBacteria:   EBBORT00000009029
- GeneID:   1195290
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0445
- NMPDR:   fig|224326.1.peg.829
- TIGR:   BB_0445
- GeneTree:   EBGT00050000006966
- HOGENOM:   HBG327581
- OMA:   EEDGVSH
- PhylomeDB:   O51401
- ProtClustDB:   PRK09197
- BioCyc:   BBUR224326:BB_0445-MONOMER
- BRENDA:   4.1.2.13
- GO:   GO:0006096
- InterPro:   IPR013785
- InterPro:   IPR006411
- InterPro:   IPR000771
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF001359
- TIGRFAMs:   TIGR00167
- TIGRFAMs:   TIGR01520

Pfam domain/function: PF01116 F_bP_aldolase

EC number: =4.1.2.13

Molecular weight: Translated: 39981; Mature: 39850

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00602 ALDOLASE_CLASS_II_1; PS00806 ALDOLASE_CLASS_II_2

Important sites: ACT_SITE 109-109 BINDING 61-61 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVLDKIKPGVVYGKELHFLYEICKKEGFAIPSINCIGTNSINAVLEAAKEINSPIMIQF
CCCCHHCCCCEEECHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEE
SNSGSAFISGKGLKMEKPQGVSIVGAISGAMHVHLMAEHYGVPVVLHTDHCAKNLLPWVE
CCCCCEEEECCCCEECCCCCCEEEEECCCCEEEEEEHHHCCCEEEEECCHHHHHHHHHHH
GLLEYGEKYYSQHKKPLFSSHMLDLSEEPIKENIEISKKFLERMAKIEMFLEIELGITGG
HHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEECCC
EEDGVDNSDRALHELFSTPEDIYYGYSELLKVSPNFQIAAAFGNVHGVYKPGNVKLTPKV
CCCCCCCHHHHHHHHHCCHHHHHHCHHHHHCCCCCCEEEEEECCCCCEECCCCEEECHHH
LKDGQDYVISKTGVNMAKPVSYVFHGGSGSTIDEINEALSYGVVKMNIDTDTQWAAWEGV
HCCCCCEEEEECCCCHHCCEEEEEECCCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHH
LNYYKKNESRLQGQLGDGKDIDIPNKKFYDPRVWLREAEVSMKDRVKIACKNLNNINRN
HHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GVLDKIKPGVVYGKELHFLYEICKKEGFAIPSINCIGTNSINAVLEAAKEINSPIMIQF
CCCHHCCCCEEECHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEE
SNSGSAFISGKGLKMEKPQGVSIVGAISGAMHVHLMAEHYGVPVVLHTDHCAKNLLPWVE
CCCCCEEEECCCCEECCCCCCEEEEECCCCEEEEEEHHHCCCEEEEECCHHHHHHHHHHH
GLLEYGEKYYSQHKKPLFSSHMLDLSEEPIKENIEISKKFLERMAKIEMFLEIELGITGG
HHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEECCC
EEDGVDNSDRALHELFSTPEDIYYGYSELLKVSPNFQIAAAFGNVHGVYKPGNVKLTPKV
CCCCCCCHHHHHHHHHCCHHHHHHCHHHHHCCCCCCEEEEEECCCCCEECCCCEEECHHH
LKDGQDYVISKTGVNMAKPVSYVFHGGSGSTIDEINEALSYGVVKMNIDTDTQWAAWEGV
HCCCCCEEEEECCCCHHCCEEEEEECCCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHH
LNYYKKNESRLQGQLGDGKDIDIPNKKFYDPRVWLREAEVSMKDRVKIACKNLNNINRN
HHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685; 2738156