Click here to switch to the map view.

The map label for this gene is atpB [H]

Identifier: 15594439

GI number: 15594439

Start: 89811

End: 91115

Strand: Reverse

Name: atpB [H]

Synonym: BB0093

Alternate gene names: 15594439

Gene position: 91115-89811 (Counterclockwise)

Preceding gene: 161511082

Following gene: 15594438

Centisome position: 10.0

GC content: 35.33

Gene sequence:

>1305_bases
ATGAAAAGAGTCTATAGTAAAATAGAGTCTATAGCAGGCAATGTAATAACTGTTACAGCTCAAGGTATTAAGTATGGTGA
GCTTGCTATTGTAAAAGCAAAAGATACAAGTTCTCTAGCCGAAGTAATTAAACTTGATCGAGAAAAAGTTTCTCTTCAGG
TTTATGGTGGTACAAGAGGTGTTTCCACGTCAGACGAGATAAAGTTTTTAGGGCATTCAATGCAGGTTTCATTTTCTGAC
AATTTGTTGGGCAGAATTTTTGATGGTTCTGGGAATCCTAGAGATGGGGGCCCTTCTCTTGATGATAATTTGATTGAAAT
TGGTGGGCCTTCTGCAAATCCTACAAAACGCATTGTTCCTAGAAATATGATAAGGACAGGGCTTCCAATGATAGATGTTT
TTAATACTCTTGTTGAATCTCAAAAATTGCCAATTTTTTCTGTTTCTGGTGAGCCTTATAATGAGCTTCTTATAAGAATT
GCACTTCAAGCAGAAGTTGATTTAATAATTCTTGGCGGAATGGGACTTAAGCATGATGATTATTTAACTTTTAAAGATTC
TTTAGAAAAGGGAGGTGCTTTAAGTAGAGCAATTTTTTTTGTTCATACTGCTAATGATTCTGTTGTTGAATCTTTAACTG
TTCCTGATATTTCACTTTCTGTTGCTGAAAAGTTTGCTCTAAAGGGCAAAAAAGTTTTGGTGCTTCTCACAGACATGACA
AATTTTGCTGATGCAATGAAAGAAATATCTATTACAATGGAACAAGTGCCTTCTAATAGAGGTTATCCCGGGGATTTGTA
TTCTCAGCTTGCATATCGTTATGAGAAGGCTATTGACTTTGAAGGCGCAGGATCAATTACAATACTTGCAGTTACAACAA
TGCCGGGTGACGATGTTACTCATCCTGTTCCTGACAATACTGGATACATTACAGAAGGTCAATACTATTTAAAAGGTGGC
AGAATAGAGCCTTTTGGGTCTCTTTCAAGACTTAAGCAAATGGTAAATAGTAGAACTAGAGACGATCACAGGACTATAAT
GGATTCAATGATCAAGCTTTATGCATCTTCAAAAGAGTCTGTAGAAAAAAAGGCTATGGGATTTAATATGACTAAGTGGG
ATGAAAAATTGCTCAAGTATAGCAATATGTTTGAAAGTAAGATGATGGATTTGTCTGTTAATATTCCTTTAGAAGAGGCT
TTAGATTTAGGTTGGAGCATTCTTGCTAGTTGTTTTAGCCCAAAAGAAACGGGAATAAAAACAGATCTTATTGAAAAATA
TTGGCCTAAAAAAGAGACTTATTGA

Upstream 100 bases:

>100_bases
TTCTTCTTTTAAGGATCATAAGTTTAATAAATTGGAGCATGCTTTGGGTGAATTGATAAATTTTAAAAAGGTAATTTAGG
GGGTTTGGAGAAATATATAT

Downstream 100 bases:

>100_bases
TTATGTCTAAAATTAAATTAACCAAAAATGATCTTAAAAAGCAAAAAGACGAACTTAAAATGTTTAAGAGATATTTGCCT
ACTTTGCAGCTTAAGAAGCA

Product: V-type ATP synthase subunit B

Products: ADP; phosphate; H+

Alternate protein names: V-ATPase subunit B [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKRVYSKIESIAGNVITVTAQGIKYGELAIVKAKDTSSLAEVIKLDREKVSLQVYGGTRGVSTSDEIKFLGHSMQVSFSD
NLLGRIFDGSGNPRDGGPSLDDNLIEIGGPSANPTKRIVPRNMIRTGLPMIDVFNTLVESQKLPIFSVSGEPYNELLIRI
ALQAEVDLIILGGMGLKHDDYLTFKDSLEKGGALSRAIFFVHTANDSVVESLTVPDISLSVAEKFALKGKKVLVLLTDMT
NFADAMKEISITMEQVPSNRGYPGDLYSQLAYRYEKAIDFEGAGSITILAVTTMPGDDVTHPVPDNTGYITEGQYYLKGG
RIEPFGSLSRLKQMVNSRTRDDHRTIMDSMIKLYASSKESVEKKAMGFNMTKWDEKLLKYSNMFESKMMDLSVNIPLEEA
LDLGWSILASCFSPKETGIKTDLIEKYWPKKETY

Sequences:

>Translated_434_residues
MKRVYSKIESIAGNVITVTAQGIKYGELAIVKAKDTSSLAEVIKLDREKVSLQVYGGTRGVSTSDEIKFLGHSMQVSFSD
NLLGRIFDGSGNPRDGGPSLDDNLIEIGGPSANPTKRIVPRNMIRTGLPMIDVFNTLVESQKLPIFSVSGEPYNELLIRI
ALQAEVDLIILGGMGLKHDDYLTFKDSLEKGGALSRAIFFVHTANDSVVESLTVPDISLSVAEKFALKGKKVLVLLTDMT
NFADAMKEISITMEQVPSNRGYPGDLYSQLAYRYEKAIDFEGAGSITILAVTTMPGDDVTHPVPDNTGYITEGQYYLKGG
RIEPFGSLSRLKQMVNSRTRDDHRTIMDSMIKLYASSKESVEKKAMGFNMTKWDEKLLKYSNMFESKMMDLSVNIPLEEA
LDLGWSILASCFSPKETGIKTDLIEKYWPKKETY
>Mature_434_residues
MKRVYSKIESIAGNVITVTAQGIKYGELAIVKAKDTSSLAEVIKLDREKVSLQVYGGTRGVSTSDEIKFLGHSMQVSFSD
NLLGRIFDGSGNPRDGGPSLDDNLIEIGGPSANPTKRIVPRNMIRTGLPMIDVFNTLVESQKLPIFSVSGEPYNELLIRI
ALQAEVDLIILGGMGLKHDDYLTFKDSLEKGGALSRAIFFVHTANDSVVESLTVPDISLSVAEKFALKGKKVLVLLTDMT
NFADAMKEISITMEQVPSNRGYPGDLYSQLAYRYEKAIDFEGAGSITILAVTTMPGDDVTHPVPDNTGYITEGQYYLKGG
RIEPFGSLSRLKQMVNSRTRDDHRTIMDSMIKLYASSKESVEKKAMGFNMTKWDEKLLKYSNMFESKMMDLSVNIPLEEA
LDLGWSILASCFSPKETGIKTDLIEKYWPKKETY

Specific function: Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit [H]

COG id: COG1156

COG function: function code C; Archaeal/vacuolar-type H+-ATPase subunit B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family [H]

Homologues:

Organism=Homo sapiens, GI19913426, Length=463, Percent_Identity=33.9092872570194, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI19913428, Length=442, Percent_Identity=35.2941176470588, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI50345984, Length=290, Percent_Identity=26.551724137931, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI4757810, Length=290, Percent_Identity=26.551724137931, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788251, Length=330, Percent_Identity=24.8484848484848, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790172, Length=328, Percent_Identity=25.609756097561, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790170, Length=264, Percent_Identity=24.6212121212121, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17570191, Length=463, Percent_Identity=35.6371490280778, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17510931, Length=426, Percent_Identity=36.8544600938967, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71988080, Length=292, Percent_Identity=27.3972602739726, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71988063, Length=292, Percent_Identity=27.3972602739726, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71988074, Length=148, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319603, Length=446, Percent_Identity=34.9775784753363, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6319370, Length=297, Percent_Identity=26.5993265993266, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281361666, Length=443, Percent_Identity=35.665914221219, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24646341, Length=443, Percent_Identity=35.665914221219, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17136796, Length=443, Percent_Identity=35.665914221219, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24658560, Length=292, Percent_Identity=26.027397260274, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR004100
- InterPro:   IPR022879 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF02874 ATP-synt_ab_N [H]

EC number: 3.6.3.14

Molecular weight: Translated: 48027; Mature: 48027

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVYSKIESIAGNVITVTAQGIKYGELAIVKAKDTSSLAEVIKLDREKVSLQVYGGTRG
CCHHHHHHHHHCCCEEEEEECCCEECCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCC
VSTSDEIKFLGHSMQVSFSDNLLGRIFDGSGNPRDGGPSLDDNLIEIGGPSANPTKRIVP
CCCCCHHHEECCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHCC
RNMIRTGLPMIDVFNTLVESQKLPIFSVSGEPYNELLIRIALQAEVDLIILGGMGLKHDD
HHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHEEEEEECCCCEEEECCCCCCCCC
YLTFKDSLEKGGALSRAIFFVHTANDSVVESLTVPDISLSVAEKFALKGKKVLVLLTDMT
CEEEHHHHHCCCCCEEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECHH
NFADAMKEISITMEQVPSNRGYPGDLYSQLAYRYEKAIDFEGAGSITILAVTTMPGDDVT
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCC
HPVPDNTGYITEGQYYLKGGRIEPFGSLSRLKQMVNSRTRDDHRTIMDSMIKLYASSKES
CCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHH
VEKKAMGFNMTKWDEKLLKYSNMFESKMMDLSVNIPLEEALDLGWSILASCFSPKETGIK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHCCHHHHHHHCCCCCCCCH
TDLIEKYWPKKETY
HHHHHHHCCCCCCC
>Mature Secondary Structure
MKRVYSKIESIAGNVITVTAQGIKYGELAIVKAKDTSSLAEVIKLDREKVSLQVYGGTRG
CCHHHHHHHHHCCCEEEEEECCCEECCEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCC
VSTSDEIKFLGHSMQVSFSDNLLGRIFDGSGNPRDGGPSLDDNLIEIGGPSANPTKRIVP
CCCCCHHHEECCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHCC
RNMIRTGLPMIDVFNTLVESQKLPIFSVSGEPYNELLIRIALQAEVDLIILGGMGLKHDD
HHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHEEEEEECCCCEEEECCCCCCCCC
YLTFKDSLEKGGALSRAIFFVHTANDSVVESLTVPDISLSVAEKFALKGKKVLVLLTDMT
CEEEHHHHHCCCCCEEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECHH
NFADAMKEISITMEQVPSNRGYPGDLYSQLAYRYEKAIDFEGAGSITILAVTTMPGDDVT
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCC
HPVPDNTGYITEGQYYLKGGRIEPFGSLSRLKQMVNSRTRDDHRTIMDSMIKLYASSKES
CCCCCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHH
VEKKAMGFNMTKWDEKLLKYSNMFESKMMDLSVNIPLEEALDLGWSILASCFSPKETGIK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHCCHHHHHHHCCCCCCCCH
TDLIEKYWPKKETY
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Borate; Diphosphate; HCO3- [C]

Metal ions: Co2+; Fe2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; H+

Specific reaction: ATP + H2O + H+(in) = ADP + phosphate + H+(out)

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Ca2+; CN-; Efrapeptin; Ethidiumbromide; Guanidines various reactions catalyzed by ATPase, analogs; Oligomycin; Quercetin; Trialkyl tinderivatives; Venturicidin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA