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The map label for this gene is ykoW

Identifier: 50812223

GI number: 50812223

Start: 1407329

End: 1409578

Strand: Reverse

Name: ykoW

Synonym: BSU13420

Alternate gene names: 50812223

Gene position: 1409578-1407329 (Counterclockwise)

Preceding gene: 16078411

Following gene: 16078406

Centisome position: 33.44

GC content: 40.58

Gene sequence:

>2250_bases
ATGGGATTCGGTATTTGGTCAATGCATTTTGTAGGGATGATGGCCGTCCATATGGAAATGCCGATGGAATATGAATTCAT
GCCTTTAATGGCAGCCATTGGCGCATCTGTTTCGGGATCATTTGTGTCATTATATTTTGTCAGCCGGCACATTCTTACAT
ATTACAGACTGCTTACCGGATCAGTTGTGCTTGGCGCATCTATTGCTTCTATGCACTATATCGGAATGTCTGCCATTTCC
CGGGTAATGATTATTTACGAACCTATTTTATTTACAGTTTCAATTATCATTGCGATTGCAGCCTCTTTCGTCTCGTTAAA
AATATTCTTTGACTTGGCCGTAAAAAAACATTCTGAGCATCTTATATTCTATAAAGGCGTCAGTTCAATTGTAATGGGCA
TTGGGATTTCGGGCATGCACTACACAGGGATGCTTGCGGCAACTTTCCATAAGGACATGGCTCCGCCCGGAAGCCATATG
GAGGTGCAAACGTTTCATTGGTCAATTTTTGTTACTTTAATTATTTTTTGTATCCAAACGTTATTGTTATTCAGTTCCCA
TGCAGATCGAAAGTTTATTAAACAGAGCGAACGCATAAAGGATAACGAACAGCGATTTCAGTCTTTAATCGTCCACAATA
TTGATGCAATTTTTATTTTATCATTAGAAGGAGACATAATATCTTCCAATCACGCTGGTGAGGAAATGATCAGCAAATTT
GGCTTCAGCATGCATGACTGGAGGAATTACACATCACTGAAGGTTAAGCGCCTTTTTGAGCAAGTTAAAAAAGATAAACA
AGCGATGAACAGCGATAGCGATCTTATCACTGAAAAAGGCCAGTTTCACTTAAATATTACGCTTATACCTGTTGAGGTCA
ATCAGGAACTAGACAGCATTTATGTCATTTGTAAAGACATGACGAAGCAGTACAAAGCAGAAAAAGAAATCCATAGAATG
GCTCATTATGATTCGCTTACAGACTTACCTAACAGAAGGCATGCCATCTCTCATCTAACAAAGGTGCTGAATCGCGAGCA
TTCCTTGCATTATAACACTGTTGTATTCTTTCTTGACTTAAACCGGTTTAAAGTGATTAACGATGCATTAGGGCATAATG
TCGGTGATCAATTGCTTCAGTTTGCAGCTAAACGCCTGTCATCTGTAGTCCCTGATAACGGATTTATCGCCCGTCTTGGC
GGTGATGAATTTATTATTATATTAACAGACGCCAATACAGGAACAGGCGAAGCAGATGTTTTAGCAAGAAAAATTATCCA
AAAATTCAAAAAACCTTTCAAAATTCAAGATCACACCCTTGTCACATCTGTCAGTATCGGGATTGCGATATCTCCAAAAG
ACGGGACGGATGGCCTTGAATTGATGAAAAAGGCTGATATGGCGATGTATGCGGCTAAAGAACGAAACAAAAGCAAATAC
AGATACTATTCTTTTTCAATCGGCAACAAAGAAACCGTTAAGCTTAACCAAGAGATGGTACTCAGAGAAGCAATCGAAAA
CGATAGATTTGTCTTGCATTATCAGCCGCAATTCAGTGTGAAAAAACAAAAAATGACAGGAGCAGAAGCACTGATACGCC
TTGTGACACCAGACGGGCAACTGCGTCCGCCCGGAGAATTCATCGGTGTTGCAGAAGAAACGGGACTTATTATTGATATC
GGAAAGTGGATTATAGATGAAGCATGCAAGCAGGCAAGGATTTGGCATGACAAAGGCTATGATCTTTCTGTCGCCATTAA
TATTTCCGCCAGACAGTTTCAATCTAAAGATCTGATTCCCCTCATCAAGGATACGCTTAATAAGTACCAGCTTCCTCCGC
AGCTTCTTGAAGTGGAGGTGACGGAAAGCATGACAATGGACAATCTCAATCACTCCAAAAAGGTTTTGTCATCCCTAACA
GAATTAGGAATCAGAATCAGTATTGACGATTTCGGTACTGGTCACAGCTCTCTTAGCTATTTGAAGGACTTCCCGATTCA
CAGGCTGAAGATTGACAAATCATTTATTGACGATATTCAGACCCATCCTAAATCAGAGCAAATTACCGGAGCTATTATTG
CCATGGGGCATCAGCTGTCTTTGCAGGTGATTGCCGAAGGTGTGGAAAATGCAGCTCAAAAGCAGCTTCTTTTTGAAAAA
GGCTGCGACCATTTGCAGGGCTTCTTTTTCAGCAGGCCAATACCTCCGGAACAATTCGAACAATTCATTATTGAACAGCC
GTCGCAATAA

Upstream 100 bases:

>100_bases
TGCCTGTACCGCCTCGTATATTTCCCTTGAACTTTCAAGGAAAGTCACCATCAATACAGGCTTAAAAAGTAAAATTTGGC
TGATCGGCGGCTCACTTATT

Downstream 100 bases:

>100_bases
CAGCGCCGGCTTTTTTTCACCCATAAGTTACTACACATGAATCGTACTCCTTCTCTTCACACTAAAGAAAAAAAGGGGTT
TATCGATATGCATACAATGT

Product: sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MGFGIWSMHFVGMMAVHMEMPMEYEFMPLMAAIGASVSGSFVSLYFVSRHILTYYRLLTGSVVLGASIASMHYIGMSAIS
RVMIIYEPILFTVSIIIAIAASFVSLKIFFDLAVKKHSEHLIFYKGVSSIVMGIGISGMHYTGMLAATFHKDMAPPGSHM
EVQTFHWSIFVTLIIFCIQTLLLFSSHADRKFIKQSERIKDNEQRFQSLIVHNIDAIFILSLEGDIISSNHAGEEMISKF
GFSMHDWRNYTSLKVKRLFEQVKKDKQAMNSDSDLITEKGQFHLNITLIPVEVNQELDSIYVICKDMTKQYKAEKEIHRM
AHYDSLTDLPNRRHAISHLTKVLNREHSLHYNTVVFFLDLNRFKVINDALGHNVGDQLLQFAAKRLSSVVPDNGFIARLG
GDEFIIILTDANTGTGEADVLARKIIQKFKKPFKIQDHTLVTSVSIGIAISPKDGTDGLELMKKADMAMYAAKERNKSKY
RYYSFSIGNKETVKLNQEMVLREAIENDRFVLHYQPQFSVKKQKMTGAEALIRLVTPDGQLRPPGEFIGVAEETGLIIDI
GKWIIDEACKQARIWHDKGYDLSVAINISARQFQSKDLIPLIKDTLNKYQLPPQLLEVEVTESMTMDNLNHSKKVLSSLT
ELGIRISIDDFGTGHSSLSYLKDFPIHRLKIDKSFIDDIQTHPKSEQITGAIIAMGHQLSLQVIAEGVENAAQKQLLFEK
GCDHLQGFFFSRPIPPEQFEQFIIEQPSQ

Sequences:

>Translated_749_residues
MGFGIWSMHFVGMMAVHMEMPMEYEFMPLMAAIGASVSGSFVSLYFVSRHILTYYRLLTGSVVLGASIASMHYIGMSAIS
RVMIIYEPILFTVSIIIAIAASFVSLKIFFDLAVKKHSEHLIFYKGVSSIVMGIGISGMHYTGMLAATFHKDMAPPGSHM
EVQTFHWSIFVTLIIFCIQTLLLFSSHADRKFIKQSERIKDNEQRFQSLIVHNIDAIFILSLEGDIISSNHAGEEMISKF
GFSMHDWRNYTSLKVKRLFEQVKKDKQAMNSDSDLITEKGQFHLNITLIPVEVNQELDSIYVICKDMTKQYKAEKEIHRM
AHYDSLTDLPNRRHAISHLTKVLNREHSLHYNTVVFFLDLNRFKVINDALGHNVGDQLLQFAAKRLSSVVPDNGFIARLG
GDEFIIILTDANTGTGEADVLARKIIQKFKKPFKIQDHTLVTSVSIGIAISPKDGTDGLELMKKADMAMYAAKERNKSKY
RYYSFSIGNKETVKLNQEMVLREAIENDRFVLHYQPQFSVKKQKMTGAEALIRLVTPDGQLRPPGEFIGVAEETGLIIDI
GKWIIDEACKQARIWHDKGYDLSVAINISARQFQSKDLIPLIKDTLNKYQLPPQLLEVEVTESMTMDNLNHSKKVLSSLT
ELGIRISIDDFGTGHSSLSYLKDFPIHRLKIDKSFIDDIQTHPKSEQITGAIIAMGHQLSLQVIAEGVENAAQKQLLFEK
GCDHLQGFFFSRPIPPEQFEQFIIEQPSQ
>Mature_748_residues
GFGIWSMHFVGMMAVHMEMPMEYEFMPLMAAIGASVSGSFVSLYFVSRHILTYYRLLTGSVVLGASIASMHYIGMSAISR
VMIIYEPILFTVSIIIAIAASFVSLKIFFDLAVKKHSEHLIFYKGVSSIVMGIGISGMHYTGMLAATFHKDMAPPGSHME
VQTFHWSIFVTLIIFCIQTLLLFSSHADRKFIKQSERIKDNEQRFQSLIVHNIDAIFILSLEGDIISSNHAGEEMISKFG
FSMHDWRNYTSLKVKRLFEQVKKDKQAMNSDSDLITEKGQFHLNITLIPVEVNQELDSIYVICKDMTKQYKAEKEIHRMA
HYDSLTDLPNRRHAISHLTKVLNREHSLHYNTVVFFLDLNRFKVINDALGHNVGDQLLQFAAKRLSSVVPDNGFIARLGG
DEFIIILTDANTGTGEADVLARKIIQKFKKPFKIQDHTLVTSVSIGIAISPKDGTDGLELMKKADMAMYAAKERNKSKYR
YYSFSIGNKETVKLNQEMVLREAIENDRFVLHYQPQFSVKKQKMTGAEALIRLVTPDGQLRPPGEFIGVAEETGLIIDIG
KWIIDEACKQARIWHDKGYDLSVAINISARQFQSKDLIPLIKDTLNKYQLPPQLLEVEVTESMTMDNLNHSKKVLSSLTE
LGIRISIDDFGTGHSSLSYLKDFPIHRLKIDKSFIDDIQTHPKSEQITGAIIAMGHQLSLQVIAEGVENAAQKQLLFEKG
CDHLQGFFFSRPIPPEQFEQFIIEQPSQ

Specific function: Probable signaling protein whose physiological role is not yet known

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain

Homologues:

Organism=Escherichia coli, GI1787541, Length=543, Percent_Identity=34.2541436464088, Blast_Score=324, Evalue=2e-89,
Organism=Escherichia coli, GI87081921, Length=438, Percent_Identity=31.7351598173516, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI226510982, Length=427, Percent_Identity=29.7423887587822, Blast_Score=206, Evalue=6e-54,
Organism=Escherichia coli, GI1790496, Length=255, Percent_Identity=36.078431372549, Blast_Score=170, Evalue=3e-43,
Organism=Escherichia coli, GI87081743, Length=242, Percent_Identity=35.9504132231405, Blast_Score=152, Evalue=1e-37,
Organism=Escherichia coli, GI87081845, Length=251, Percent_Identity=30.6772908366534, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786507, Length=237, Percent_Identity=30.8016877637131, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1788502, Length=256, Percent_Identity=30.078125, Blast_Score=123, Evalue=5e-29,
Organism=Escherichia coli, GI87081980, Length=237, Percent_Identity=32.4894514767932, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788849, Length=434, Percent_Identity=26.2672811059908, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1788381, Length=434, Percent_Identity=25.3456221198157, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1787055, Length=238, Percent_Identity=30.672268907563, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87082096, Length=255, Percent_Identity=27.843137254902, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=34.1935483870968, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1787262, Length=183, Percent_Identity=28.4153005464481, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787802, Length=176, Percent_Identity=34.0909090909091, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI87081881, Length=199, Percent_Identity=31.1557788944724, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1786584, Length=192, Percent_Identity=25.5208333333333, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=32.7272727272727, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI87082007, Length=186, Percent_Identity=32.258064516129, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693134, Length=171, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1789650, Length=454, Percent_Identity=18.7224669603524, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=31.7073170731707, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=26.3473053892216, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YKOW_BACSU (O34311)

Other databases:

- EMBL:   AL009126
- PIR:   A69861
- RefSeq:   YP_054577.1
- ProteinModelPortal:   O34311
- EnsemblBacteria:   EBBACT00000000592
- GeneID:   2914252
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU13420
- NMPDR:   fig|224308.1.peg.1344
- GenoList:   BSU13420
- GeneTree:   EBGT00070000031763
- HOGENOM:   HBG751764
- PhylomeDB:   O34311
- ProtClustDB:   CLSK928128
- BioCyc:   BSUB:BSU13420-MONOMER
- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330
- InterPro:   IPR000014
- SMART:   SM00052
- SMART:   SM00267
- SMART:   SM00091
- TIGRFAMs:   TIGR00254

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT; SSF55073 A/G_cyclase

EC number: NA

Molecular weight: Translated: 84818; Mature: 84686

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS50883 EAL; PS50887 GGDEF; PS50924 MHYT; PS50112 PAS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe3da454)-; HASH(0xddbc938)-; HASH(0xe1293f0)-; HASH(0xe7133fc)-; HASH(0xdff5628)-; HASH(0xe6c30e8)-; HASH(0xe126c98)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFGIWSMHFVGMMAVHMEMPMEYEFMPLMAAIGASVSGSFVSLYFVSRHILTYYRLLTG
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
SVVLGASIASMHYIGMSAISRVMIIYEPILFTVSIIIAIAASFVSLKIFFDLAVKKHSEH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LIFYKGVSSIVMGIGISGMHYTGMLAATFHKDMAPPGSHMEVQTFHWSIFVTLIIFCIQT
EEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
LLLFSSHADRKFIKQSERIKDNEQRFQSLIVHNIDAIFILSLEGDIISSNHAGEEMISKF
HHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCEECCCCCCHHHHHHH
GFSMHDWRNYTSLKVKRLFEQVKKDKQAMNSDSDLITEKGQFHLNITLIPVEVNQELDSI
CCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEEEEEEEEEECCCCCHHH
YVICKDMTKQYKAEKEIHRMAHYDSLTDLPNRRHAISHLTKVLNREHSLHYNTVVFFLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEC
NRFKVINDALGHNVGDQLLQFAAKRLSSVVPDNGFIARLGGDEFIIILTDANTGTGEADV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCHHHH
LARKIIQKFKKPFKIQDHTLVTSVSIGIAISPKDGTDGLELMKKADMAMYAAKERNKSKY
HHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
RYYSFSIGNKETVKLNQEMVLREAIENDRFVLHYQPQFSVKKQKMTGAEALIRLVTPDGQ
EEEEEECCCCCEEEECHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCHHHEEEEECCCCC
LRPPGEFIGVAEETGLIIDIGKWIIDEACKQARIWHDKGYDLSVAINISARQFQSKDLIP
CCCCHHHEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHCCCCCCHH
LIKDTLNKYQLPPQLLEVEVTESMTMDNLNHSKKVLSSLTELGIRISIDDFGTGHSSLSY
HHHHHHHHHCCCHHHEEEEHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHH
LKDFPIHRLKIDKSFIDDIQTHPKSEQITGAIIAMGHQLSLQVIAEGVENAAQKQLLFEK
HHCCCCCEEEECHHHHHHHHCCCCCCHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
GCDHLQGFFFSRPIPPEQFEQFIIEQPSQ
CCHHHHHHHHCCCCCHHHHHHHHHCCCCH
>Mature Secondary Structure 
GFGIWSMHFVGMMAVHMEMPMEYEFMPLMAAIGASVSGSFVSLYFVSRHILTYYRLLTG
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHC
SVVLGASIASMHYIGMSAISRVMIIYEPILFTVSIIIAIAASFVSLKIFFDLAVKKHSEH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LIFYKGVSSIVMGIGISGMHYTGMLAATFHKDMAPPGSHMEVQTFHWSIFVTLIIFCIQT
EEEECCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
LLLFSSHADRKFIKQSERIKDNEQRFQSLIVHNIDAIFILSLEGDIISSNHAGEEMISKF
HHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCEECCCCCCHHHHHHH
GFSMHDWRNYTSLKVKRLFEQVKKDKQAMNSDSDLITEKGQFHLNITLIPVEVNQELDSI
CCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCEEEEEEEEEEEECCCCCHHH
YVICKDMTKQYKAEKEIHRMAHYDSLTDLPNRRHAISHLTKVLNREHSLHYNTVVFFLDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEC
NRFKVINDALGHNVGDQLLQFAAKRLSSVVPDNGFIARLGGDEFIIILTDANTGTGEADV
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECCCCCCCHHHH
LARKIIQKFKKPFKIQDHTLVTSVSIGIAISPKDGTDGLELMKKADMAMYAAKERNKSKY
HHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCE
RYYSFSIGNKETVKLNQEMVLREAIENDRFVLHYQPQFSVKKQKMTGAEALIRLVTPDGQ
EEEEEECCCCCEEEECHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCHHHEEEEECCCCC
LRPPGEFIGVAEETGLIIDIGKWIIDEACKQARIWHDKGYDLSVAINISARQFQSKDLIP
CCCCHHHEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEHHHCCCCCCHH
LIKDTLNKYQLPPQLLEVEVTESMTMDNLNHSKKVLSSLTELGIRISIDDFGTGHSSLSY
HHHHHHHHHCCCHHHEEEEHHHHHHHCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHH
LKDFPIHRLKIDKSFIDDIQTHPKSEQITGAIIAMGHQLSLQVIAEGVENAAQKQLLFEK
HHCCCCCEEEECHHHHHHHHCCCCCCHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
GCDHLQGFFFSRPIPPEQFEQFIIEQPSQ
CCHHHHHHHHCCCCCHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11728710