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The map label for this gene is nupC

Identifier: 255767837

GI number: 255767837

Start: 4050340

End: 4051521

Strand: Reverse

Name: nupC

Synonym: BSU39410

Alternate gene names: 255767837

Gene position: 4051521-4050340 (Counterclockwise)

Preceding gene: 255767838

Following gene: 50812307

Centisome position: 96.11

GC content: 43.23

Gene sequence:

>1182_bases
ATGAAGTATTTGATTGGGATTATCGGTTTAATCGTGTTTTTAGGCCTCGCGTGGATCGCGAGCAGCGGCAAAAAAAGAAT
TAAGATCCGCCCAATTGTTGTTATGCTCATTTTGCAATTTATTCTTGGCTACATTCTCCTCAATACCGGAATAGGGAATT
TCCTCGTGGGAGGATTTGCAAAAGGATTCGGTTACCTGCTTGAATACGCGGCAGAGGGAATTAACTTTGTGTTTGGCGGC
TTGGTGAATGCGGACCAAACGACATTCTTTATGAATGTTCTCTTGCCAATCGTGTTTATTTCCGCTCTGATCGGGATTCT
GCAAAAGTGGAAAGTCCTCCCGTTTATCATTAGATATATCGGCCTTGCCCTCAGCAAGGTAAACGGTATGGGAAGATTGG
AATCGTATAACGCAGTGGCTTCTGCGATTTTAGGGCAGTCAGAAGTATTTATCTCCTTGAAGAAAGAACTCGGTCTTTTA
AATCAGCAGCGCTTGTACACGCTTTGCGCATCTGCGATGTCAACTGTATCAATGTCGATTGTCGGTGCGTATATGACAAT
GCTGAAACCGGAATATGTTGTAACAGCGCTTGTTTTGAACTTATTTGGCGGTTTCATTATCGCTTCTATTATCAATCCGT
ACGAGGTTGCAAAAGAAGAGGATATGCTTCGTGTTGAGGAAGAAGAAAAACAATCCTTCTTCGAAGTGCTCGGAGAATAC
ATTCTTGACGGTTTCAAAGTAGCGGTTGTCGTCGCTGCGATGCTGATTGGATTTGTCGCGATTATTGCATTGATCAATGG
CATTTTTAATGCAGTATTCGGTATTTCGTTCCAAGGCATTCTTGGATATGTGTTTGCTCCATTCGCTTTTCTTGTCGGTA
TCCCATGGAATGAAGCTGTTAATGCGGGAAGCATTATGGCAACAAAAATGGTATCGAATGAATTTGTCGCCATGACGTCG
CTTACGCAAAACGGTTTCCATTTCAGCGGCCGTACAACAGCGATCGTATCGGTATTCCTTGTGTCATTTGCGAACTTCTC
CTCAATCGGAATCATTGCCGGTGCCGTAAAAGGACTGAATGAAAAGCAAGGAAATGTCGTCGCTCGTTTCGGCTTGAAAT
TATTATACGGTGCTACGCTTGTCAGCTTTTTATCAGCAGCAATTGTGGGCTTGATTTACTGA

Upstream 100 bases:

>100_bases
CAGGCGGAGACAACTATTAAGAGCTGACGGAAGGCAGACTGCTTTCCGTTTTCTGAGGAAACAGTAATTGTAAGCGAATA
CAAATAAAGGAGAAACACAT

Downstream 100 bases:

>100_bases
ACTTAATCGAAAAGGATCGGTGACCAAACATGAGAATGGTAGATATCATCATCAAAAAACAAAACGGAAAAGAACTCACC
ACTGAAGAAATTCAATTTTT

Product: pyrimidine-nucleoside Na+(H+) cotransporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKYLIGIIGLIVFLGLAWIASSGKKRIKIRPIVVMLILQFILGYILLNTGIGNFLVGGFAKGFGYLLEYAAEGINFVFGG
LVNADQTTFFMNVLLPIVFISALIGILQKWKVLPFIIRYIGLALSKVNGMGRLESYNAVASAILGQSEVFISLKKELGLL
NQQRLYTLCASAMSTVSMSIVGAYMTMLKPEYVVTALVLNLFGGFIIASIINPYEVAKEEDMLRVEEEEKQSFFEVLGEY
ILDGFKVAVVVAAMLIGFVAIIALINGIFNAVFGISFQGILGYVFAPFAFLVGIPWNEAVNAGSIMATKMVSNEFVAMTS
LTQNGFHFSGRTTAIVSVFLVSFANFSSIGIIAGAVKGLNEKQGNVVARFGLKLLYGATLVSFLSAAIVGLIY

Sequences:

>Translated_393_residues
MKYLIGIIGLIVFLGLAWIASSGKKRIKIRPIVVMLILQFILGYILLNTGIGNFLVGGFAKGFGYLLEYAAEGINFVFGG
LVNADQTTFFMNVLLPIVFISALIGILQKWKVLPFIIRYIGLALSKVNGMGRLESYNAVASAILGQSEVFISLKKELGLL
NQQRLYTLCASAMSTVSMSIVGAYMTMLKPEYVVTALVLNLFGGFIIASIINPYEVAKEEDMLRVEEEEKQSFFEVLGEY
ILDGFKVAVVVAAMLIGFVAIIALINGIFNAVFGISFQGILGYVFAPFAFLVGIPWNEAVNAGSIMATKMVSNEFVAMTS
LTQNGFHFSGRTTAIVSVFLVSFANFSSIGIIAGAVKGLNEKQGNVVARFGLKLLYGATLVSFLSAAIVGLIY
>Mature_393_residues
MKYLIGIIGLIVFLGLAWIASSGKKRIKIRPIVVMLILQFILGYILLNTGIGNFLVGGFAKGFGYLLEYAAEGINFVFGG
LVNADQTTFFMNVLLPIVFISALIGILQKWKVLPFIIRYIGLALSKVNGMGRLESYNAVASAILGQSEVFISLKKELGLL
NQQRLYTLCASAMSTVSMSIVGAYMTMLKPEYVVTALVLNLFGGFIIASIINPYEVAKEEDMLRVEEEEKQSFFEVLGEY
ILDGFKVAVVVAAMLIGFVAIIALINGIFNAVFGISFQGILGYVFAPFAFLVGIPWNEAVNAGSIMATKMVSNEFVAMTS
LTQNGFHFSGRTTAIVSVFLVSFANFSSIGIIAGAVKGLNEKQGNVVARFGLKLLYGATLVSFLSAAIVGLIY

Specific function: Transports Nucleosides With A High Affinity Except Guanosine And Deoxyguanosine. Driven By A Proton Motive Force. [C]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family

Homologues:

Organism=Homo sapiens, GI42542381, Length=420, Percent_Identity=25.2380952380952, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI11545853, Length=419, Percent_Identity=25.7756563245823, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI227116277, Length=418, Percent_Identity=22.9665071770335, Blast_Score=109, Evalue=5e-24,
Organism=Escherichia coli, GI1788737, Length=392, Percent_Identity=60.2040816326531, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1788485, Length=410, Percent_Identity=32.6829268292683, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788488, Length=410, Percent_Identity=33.1707317073171, Blast_Score=181, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17560276, Length=391, Percent_Identity=24.5524296675192, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71991794, Length=405, Percent_Identity=21.7283950617284, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45552519, Length=409, Percent_Identity=23.7163814180929, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45552517, Length=409, Percent_Identity=23.7163814180929, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19921868, Length=409, Percent_Identity=23.7163814180929, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281360430, Length=337, Percent_Identity=24.3323442136499, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUPC_BACSU (P39141)

Other databases:

- EMBL:   X82174
- EMBL:   D45912
- EMBL:   AL009126
- PIR:   S78769
- RefSeq:   NP_391820.2
- EnsemblBacteria:   EBBACT00000001425
- GeneID:   937534
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39410
- NMPDR:   fig|224308.1.peg.3946
- GenoList:   BSU39410
- GeneTree:   EBGT00070000031826
- HOGENOM:   HBG713614
- PhylomeDB:   P39141
- ProtClustDB:   CLSK2523464
- BioCyc:   BSUB:BSU39410-MONOMER
- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668
- PANTHER:   PTHR10590
- TIGRFAMs:   TIGR00804

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N

EC number: NA

Molecular weight: Translated: 42496; Mature: 42496

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13583e1c)-; HASH(0x13218e40)-; HASH(0x13601f3c)-; HASH(0x13616838)-; HASH(0x134eab28)-; HASH(0x13568d00)-; HASH(0x136536c0)-; HASH(0x135ae448)-; HASH(0x11fea8a4)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure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CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8550462; 8867804; 9384377