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The map label for this gene is narK

Identifier: 255767791

GI number: 255767791

Start: 3832327

End: 3833514

Strand: Reverse

Name: narK

Synonym: BSU37320

Alternate gene names: 255767791

Gene position: 3833514-3832327 (Counterclockwise)

Preceding gene: 16080786

Following gene: 16080784

Centisome position: 90.94

GC content: 47.31

Gene sequence:

>1188_bases
ATGATCAACCGTCAACACATTCAATTATCGTTACAGTCATTAAGCTTGGTGGCAGGGTTTATGGTTTGGGTGCTGATTTC
ATCACTCATTTCCCAAATCACATTAGATATTCATTTAAGCAAGGGTGAGATTTCTTTAGTGACCGCGATTCCTGTGATCC
TCGGGTCGCTTCTCCGCATTCCTTTAGGGTATTTAACGAACAGGTTTGGCGCGCGGCTCATGTTTATGGTCAGCTTCATC
CTGCTTTTATTTCCTGTATTTTGGATCAGTATCGCGGATTCTCTGTTTGATTTAATCGCAGGCGGCTTTTTCTTAGGGAT
CGGGGGAGCGGTGTTTTCCATTGGGGTGACCTCCCTCCCGAAATATTATCCGAAAGAAAAGCACGGTGTCGTCAATGGGA
TTTACGGTGCAGGAAATATCGGGACAGCCGTTACGACATTTGCAGCGCCGGTTATCGCTCAAGCCGTCGGGTGGAAATCA
ACCGTGCAAATGTATCTGATTTTGCTGGCTGTCTTTGCCTTACTGCACGTATTGTTTGGCGACAGGCATGAGAAGAAGGT
CAAAGTTTCTGTTAAGACTCAAATCAAAGCTGTTTACCGAAATCATGTGCTATGGTTTCTGAGCCTGTTTTATTTTATTA
CGTTCGGTGCCTTTGTCGCCTTCACTATCTATCTGCCGAACTTTCTGGTGGAGCATTTTGGGCTGAATCCTGCAGATGCC
GGCTTGCGGACGGCTGGCTTTATTGCGGTTTCTACGCTGCTCAGGCCTGCGGGCGGATTCCTGGCTGATAAAATGAGCCC
GCTCCGCATCTTGATGTTTGTTTTTGCCGGGCTTACATTATCAGGCATTATCCTATCGTTCTCGCCGACAATCGGCCTTT
ATACGTTTGGATCATTAACCGTGGCCGTCTGCTCAGGCATAGGAAACGGAACTGTTTTTAAACTGGTGCCTTTCTATTTT
TCGAAGCAAGCGGGGATTGCCAATGGGATTGTGTCTGCCATGGGCGGTTTAGGCGGCTTTTTTCCTCCGCTCATATTGGC
GAGTGTATTTCAGGCAACAGGCCAGTACGCGATCGGGTTTATGGCGCTTTCAGAGGTGGCGTTAGCCAGTTTTGTCCTTG
TCATATGGATGTATTGGCAGGAAAGAATGAAAACACACACGGAGAGAAACAGCCAGAGCATCAACTAA

Upstream 100 bases:

>100_bases
CTTTTTTTATGAGAAAAACGTGTGATGTAATTCACAATCCTGTTTGGCTAGTTTTTGTATGATAAGACTGATTATTGAAT
CATTGAAAGGGGCAAGCCAA

Downstream 100 bases:

>100_bases
CACTGGGGCATTCACAAGATTGTTAGTTTTTTCTCGATGGGTACAAATCAATTCACGTTACAATAAACACACAACGTGAC
TTTCTTCGAGGTGAGCTCAT

Product: nitrite extrusion permease

Products: NA

Alternate protein names: Nitrite facilitator

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MINRQHIQLSLQSLSLVAGFMVWVLISSLISQITLDIHLSKGEISLVTAIPVILGSLLRIPLGYLTNRFGARLMFMVSFI
LLLFPVFWISIADSLFDLIAGGFFLGIGGAVFSIGVTSLPKYYPKEKHGVVNGIYGAGNIGTAVTTFAAPVIAQAVGWKS
TVQMYLILLAVFALLHVLFGDRHEKKVKVSVKTQIKAVYRNHVLWFLSLFYFITFGAFVAFTIYLPNFLVEHFGLNPADA
GLRTAGFIAVSTLLRPAGGFLADKMSPLRILMFVFAGLTLSGIILSFSPTIGLYTFGSLTVAVCSGIGNGTVFKLVPFYF
SKQAGIANGIVSAMGGLGGFFPPLILASVFQATGQYAIGFMALSEVALASFVLVIWMYWQERMKTHTERNSQSIN

Sequences:

>Translated_395_residues
MINRQHIQLSLQSLSLVAGFMVWVLISSLISQITLDIHLSKGEISLVTAIPVILGSLLRIPLGYLTNRFGARLMFMVSFI
LLLFPVFWISIADSLFDLIAGGFFLGIGGAVFSIGVTSLPKYYPKEKHGVVNGIYGAGNIGTAVTTFAAPVIAQAVGWKS
TVQMYLILLAVFALLHVLFGDRHEKKVKVSVKTQIKAVYRNHVLWFLSLFYFITFGAFVAFTIYLPNFLVEHFGLNPADA
GLRTAGFIAVSTLLRPAGGFLADKMSPLRILMFVFAGLTLSGIILSFSPTIGLYTFGSLTVAVCSGIGNGTVFKLVPFYF
SKQAGIANGIVSAMGGLGGFFPPLILASVFQATGQYAIGFMALSEVALASFVLVIWMYWQERMKTHTERNSQSIN
>Mature_395_residues
MINRQHIQLSLQSLSLVAGFMVWVLISSLISQITLDIHLSKGEISLVTAIPVILGSLLRIPLGYLTNRFGARLMFMVSFI
LLLFPVFWISIADSLFDLIAGGFFLGIGGAVFSIGVTSLPKYYPKEKHGVVNGIYGAGNIGTAVTTFAAPVIAQAVGWKS
TVQMYLILLAVFALLHVLFGDRHEKKVKVSVKTQIKAVYRNHVLWFLSLFYFITFGAFVAFTIYLPNFLVEHFGLNPADA
GLRTAGFIAVSTLLRPAGGFLADKMSPLRILMFVFAGLTLSGIILSFSPTIGLYTFGSLTVAVCSGIGNGTVFKLVPFYF
SKQAGIANGIVSAMGGLGGFFPPLILASVFQATGQYAIGFMALSEVALASFVLVIWMYWQERMKTHTERNSQSIN

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family

Homologues:

Organism=Escherichia coli, GI1787475, Length=441, Percent_Identity=25.1700680272109, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787743, Length=443, Percent_Identity=25.0564334085779, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NARK_BACSU (P46907)

Other databases:

- EMBL:   Z49884
- EMBL:   AL009126
- EMBL:   Z97024
- PIR:   S60080
- RefSeq:   NP_391613.2
- ProteinModelPortal:   P46907
- EnsemblBacteria:   EBBACT00000003910
- GeneID:   937055
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU37320
- NMPDR:   fig|224308.1.peg.3739
- GenoList:   BSU37320
- GeneTree:   EBGT00050000000110
- HOGENOM:   HBG479449
- PhylomeDB:   P46907
- ProtClustDB:   CLSK885946
- BioCyc:   BSUB:BSU37320-MONOMER
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 42927; Mature: 42927

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12ffe3c4)-; HASH(0x1325497c)-; HASH(0x1324fac4)-; HASH(0x12cf5dcc)-; HASH(0x12a12fc8)-; HASH(0xf971934)-; HASH(0x12fed92c)-; HASH(0x1324fcc8)-; HASH(0x130b2af0)-; HASH(0x1324fcbc)-; HASH(0x1324fc44)-; HASH(0x13256ab0)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933