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The map label for this gene is aapA

Identifier: 255767625

GI number: 255767625

Start: 2766558

End: 2767946

Strand: Direct

Name: aapA

Synonym: BSU27090

Alternate gene names: 255767625

Gene position: 2766558-2767946 (Clockwise)

Preceding gene: 50812271

Following gene: 16079765

Centisome position: 65.63

GC content: 42.12

Gene sequence:

>1389_bases
TTGATAGGCAATTCTAGCAAAGACAATTTTGGCCAGCAACAGAAATTGTCTAGAGGCCTTAAAAACAGGCATATACAATT
AATGGCGATTGGAGGTGCAATCGGTACAGGTTTATTTTTAGGTTCAGGTAAATCCATCCATTTTGCAGGACCATCCATTT
TATTTGCTTACTTGATCACCGGCGTATTTTGCTTTTTCATTATGCGTTCGCTCGGAGAACTGCTCTTATCAAATGCGGGA
TACCACTCTTTTGTTGATTTTGTAAGGGACTATTTAGGAAACATGGCAGCATTTATCACTGGGTGGACCTACTGGTTCTG
TTGGATTTCCCTCGCGATGGCTGATTTAACGGCGGTCGGCATTTATACGCAATATTGGCTTCCCGATGTGCCTCAATGGC
TGCCGGGTCTTCTTGCGCTTATTATCCTGCTGATCATGAACCTTGCCACCGTTAAACTTTTCGGAGAATTAGAATTTTGG
TTTGCATTGATCAAAGTCATTGCCATACTGGCTCTGATCGTAACTGGTATTCTCCTGATTGCGAAAGGTTTTTCCGCAGC
TTCCGGCCCTGCCAGCCTCAACAACCTTTGGAGCCACGGCGGCATGTTCCCAAATGGATGGCATGGGTTTATCCTATCTT
TCCAAATGGTCGTCTTCGCTTTCGTGGGTATTGAACTTGTTGGACTGACAGCAGGTGAAACAGAAAATCCCCAAAAAGTG
ATCCCTAAAGCCATCAATCAAATTCCTGTCCGGATTTTACTTTTTTATGTTGGCGCACTTTTCGTGATTATGTGTATCTA
TCCTTGGAATGTGTTGAATCCAAACGAAAGCCCATTTGTTCAGGTCTTCTCTGCAGTAGGCATCGTTGTGGCTGCCAGTT
TGATCAATTTTGTTGTATTAACATCAGCCGCATCAGCTGCAAATAGCGCTTTATTCAGCACAAGCCGAATGGTCTATTCA
CTTGCAAAGGATCATCACGCACCTGGACTATTAAAGAAATTAACTTCTTCTAACGTGCCAAGCAATGCGCTGTTCTTTTC
ATCCATCGCCATTCTGATTGGCGTTAGTCTGAATTATTTAATGCCCGAGCAAGTCTTCACGCTGATTACAAGTGTTTCAA
CAATATGCTTCATTTTTATTTGGGGCATCACGGTGATTTGCCATTTGAAATATCGAAAAACAAGGCAGCATGAAGCGAAA
GCAAACAAATTTAAAATGCCTTTTTACCCATTATCCAACTATTTAACACTTGCATTTCTCGCGTTCATTCTTGTCATATT
GGCACTGGCGAATGATACACGTATTGCTTTGTTTGTCACCCCGGTCTGGTTTGTTTTATTGATTATTTTGTATAAGGTGC
AAACCCGCAGAGGACATAAGGTGAAATAA

Upstream 100 bases:

>100_bases
AAATAGTTTTAAAATTTAAAAATAAAAGTATCTATTCTTTTAAATGTTATAATATCCGATAAAACTTTACTCATCTTTTT
ATTACTGGAGGAAAATAAAA

Downstream 100 bases:

>100_bases
AAAAAAGACAAGGGATACTCACTCCCTTGTCTTTTTATATGTGTCAAACACTAAATTAAACTTTGGTTTCAAATTTCATT
CACTTCATTTAAGTGTTAAC

Product: d-Serine/d-alanine/glycine permease

Products: D-glycine [Cytoplasm]; D-alanine [Cytoplasm]; Proton [Cytoplasm]; D-serine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MIGNSSKDNFGQQQKLSRGLKNRHIQLMAIGGAIGTGLFLGSGKSIHFAGPSILFAYLITGVFCFFIMRSLGELLLSNAG
YHSFVDFVRDYLGNMAAFITGWTYWFCWISLAMADLTAVGIYTQYWLPDVPQWLPGLLALIILLIMNLATVKLFGELEFW
FALIKVIAILALIVTGILLIAKGFSAASGPASLNNLWSHGGMFPNGWHGFILSFQMVVFAFVGIELVGLTAGETENPQKV
IPKAINQIPVRILLFYVGALFVIMCIYPWNVLNPNESPFVQVFSAVGIVVAASLINFVVLTSAASAANSALFSTSRMVYS
LAKDHHAPGLLKKLTSSNVPSNALFFSSIAILIGVSLNYLMPEQVFTLITSVSTICFIFIWGITVICHLKYRKTRQHEAK
ANKFKMPFYPLSNYLTLAFLAFILVILALANDTRIALFVTPVWFVLLIILYKVQTRRGHKVK

Sequences:

>Translated_462_residues
MIGNSSKDNFGQQQKLSRGLKNRHIQLMAIGGAIGTGLFLGSGKSIHFAGPSILFAYLITGVFCFFIMRSLGELLLSNAG
YHSFVDFVRDYLGNMAAFITGWTYWFCWISLAMADLTAVGIYTQYWLPDVPQWLPGLLALIILLIMNLATVKLFGELEFW
FALIKVIAILALIVTGILLIAKGFSAASGPASLNNLWSHGGMFPNGWHGFILSFQMVVFAFVGIELVGLTAGETENPQKV
IPKAINQIPVRILLFYVGALFVIMCIYPWNVLNPNESPFVQVFSAVGIVVAASLINFVVLTSAASAANSALFSTSRMVYS
LAKDHHAPGLLKKLTSSNVPSNALFFSSIAILIGVSLNYLMPEQVFTLITSVSTICFIFIWGITVICHLKYRKTRQHEAK
ANKFKMPFYPLSNYLTLAFLAFILVILALANDTRIALFVTPVWFVLLIILYKVQTRRGHKVK
>Mature_462_residues
MIGNSSKDNFGQQQKLSRGLKNRHIQLMAIGGAIGTGLFLGSGKSIHFAGPSILFAYLITGVFCFFIMRSLGELLLSNAG
YHSFVDFVRDYLGNMAAFITGWTYWFCWISLAMADLTAVGIYTQYWLPDVPQWLPGLLALIILLIMNLATVKLFGELEFW
FALIKVIAILALIVTGILLIAKGFSAASGPASLNNLWSHGGMFPNGWHGFILSFQMVVFAFVGIELVGLTAGETENPQKV
IPKAINQIPVRILLFYVGALFVIMCIYPWNVLNPNESPFVQVFSAVGIVVAASLINFVVLTSAASAANSALFSTSRMVYS
LAKDHHAPGLLKKLTSSNVPSNALFFSSIAILIGVSLNYLMPEQVFTLITSVSTICFIFIWGITVICHLKYRKTRQHEAK
ANKFKMPFYPLSNYLTLAFLAFILVILALANDTRIALFVTPVWFVLLIILYKVQTRRGHKVK

Specific function: Probable amino-acid or metabolite transport protein

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily

Homologues:

Organism=Homo sapiens, GI115648063, Length=489, Percent_Identity=23.9263803680982, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI115648022, Length=489, Percent_Identity=23.9263803680982, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4507047, Length=352, Percent_Identity=25.2840909090909, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI181337167, Length=335, Percent_Identity=23.5820895522388, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI110347453, Length=350, Percent_Identity=24.5714285714286, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1790653, Length=445, Percent_Identity=53.2584269662921, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1786789, Length=453, Percent_Identity=42.8256070640177, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786302, Length=455, Percent_Identity=41.7582417582418, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI48994972, Length=461, Percent_Identity=40.7809110629067, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1786602, Length=451, Percent_Identity=44.7893569844789, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI87081915, Length=406, Percent_Identity=39.6551724137931, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI1789017, Length=417, Percent_Identity=33.5731414868106, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87081708, Length=433, Percent_Identity=34.8729792147806, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1788480, Length=435, Percent_Identity=34.9425287356322, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI87081869, Length=420, Percent_Identity=22.3809523809524, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17540018, Length=463, Percent_Identity=23.3261339092873, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320772, Length=450, Percent_Identity=30.8888888888889, Blast_Score=202, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6324061, Length=448, Percent_Identity=29.6875, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324924, Length=476, Percent_Identity=31.5126050420168, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322892, Length=438, Percent_Identity=29.9086757990868, Blast_Score=176, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6324990, Length=367, Percent_Identity=33.5149863760218, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6321053, Length=433, Percent_Identity=28.175519630485, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324059, Length=440, Percent_Identity=30.2272727272727, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319824, Length=423, Percent_Identity=26.4775413711584, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320717, Length=419, Percent_Identity=25.7756563245823, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6321629, Length=431, Percent_Identity=27.1461716937355, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319543, Length=401, Percent_Identity=26.9326683291771, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319608, Length=430, Percent_Identity=26.5116279069767, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320251, Length=409, Percent_Identity=25.4278728606357, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319542, Length=411, Percent_Identity=25.0608272506083, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6324553, Length=410, Percent_Identity=27.3170731707317, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6322967, Length=409, Percent_Identity=24.6943765281174, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324981, Length=406, Percent_Identity=26.6009852216749, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320364, Length=493, Percent_Identity=26.369168356998, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI161077963, Length=370, Percent_Identity=24.0540540540541, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221512776, Length=335, Percent_Identity=22.089552238806, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24666159, Length=335, Percent_Identity=22.089552238806, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AAPA_BACSU (O06005)

Other databases:

- EMBL:   X92868
- EMBL:   AL009126
- PIR:   B69580
- RefSeq:   NP_390587.2
- ProteinModelPortal:   O06005
- EnsemblBacteria:   EBBACT00000002470
- GeneID:   937231
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU27090
- NMPDR:   fig|224308.1.peg.2712
- GenoList:   BSU27090
- GeneTree:   EBGT00070000031860
- HOGENOM:   HBG492579
- PhylomeDB:   O06005
- ProtClustDB:   CLSK882815
- BioCyc:   BSUB:BSU27090-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 51018; Mature: 51018

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x113a4ec8)-; HASH(0xd217cd8)-; HASH(0x1143b244)-; HASH(0x1024d180)-; HASH(0x102e6d70)-; HASH(0x114455f4)-; HASH(0x114075fc)-; HASH(0x1130b5d4)-; HASH(0xfc70568)-; HASH(0x102e6e9c)-; HASH(0x106d62ec)-; HASH(0x114455a0)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGNSSKDNFGQQQKLSRGLKNRHIQLMAIGGAIGTGLFLGSGKSIHFAGPSILFAYLIT
CCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCHHHHHHEECCCCEEEECCHHHHHHHHHH
GVFCFFIMRSLGELLLSNAGYHSFVDFVRDYLGNMAAFITGWTYWFCWISLAMADLTAVG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYTQYWLPDVPQWLPGLLALIILLIMNLATVKLFGELEFWFALIKVIAILALIVTGILLI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGFSAASGPASLNNLWSHGGMFPNGWHGFILSFQMVVFAFVGIELVGLTAGETENPQKV
HCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHH
IPKAINQIPVRILLFYVGALFVIMCIYPWNVLNPNESPFVQVFSAVGIVVAASLINFVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TSAASAANSALFSTSRMVYSLAKDHHAPGLLKKLTSSNVPSNALFFSSIAILIGVSLNYL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
MPEQVFTLITSVSTICFIFIWGITVICHLKYRKTRQHEAKANKFKMPFYPLSNYLTLAFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AFILVILALANDTRIALFVTPVWFVLLIILYKVQTRRGHKVK
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MIGNSSKDNFGQQQKLSRGLKNRHIQLMAIGGAIGTGLFLGSGKSIHFAGPSILFAYLIT
CCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCHHHHHHEECCCCEEEECCHHHHHHHHHH
GVFCFFIMRSLGELLLSNAGYHSFVDFVRDYLGNMAAFITGWTYWFCWISLAMADLTAVG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYTQYWLPDVPQWLPGLLALIILLIMNLATVKLFGELEFWFALIKVIAILALIVTGILLI
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKGFSAASGPASLNNLWSHGGMFPNGWHGFILSFQMVVFAFVGIELVGLTAGETENPQKV
HCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHH
IPKAINQIPVRILLFYVGALFVIMCIYPWNVLNPNESPFVQVFSAVGIVVAASLINFVVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TSAASAANSALFSTSRMVYSLAKDHHAPGLLKKLTSSNVPSNALFFSSIAILIGVSLNYL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
MPEQVFTLITSVSTICFIFIWGITVICHLKYRKTRQHEAKANKFKMPFYPLSNYLTLAFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AFILVILALANDTRIALFVTPVWFVLLIILYKVQTRRGHKVK
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glycine [Periplasm]; D-alanine [Periplasm]; Proton [Periplasm]; D-serine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + D-glycine [Periplasm] = Proton [Cytoplasm] + D-glycine [Cytoplasm] Proton [Periplasm] + D-alanine [Periplasm] = Proton [Cytoplasm] + D-alanine [Cytoplasm] Proton [Periplasm] + D-serine [Periplasm] = Proton [Cytoplasm] + D-serine [Cyto

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9141695; 9384377