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The map label for this gene is yqjE

Identifier: 255767520

GI number: 255767520

Start: 2485680

End: 2486795

Strand: Reverse

Name: yqjE

Synonym: BSU23910

Alternate gene names: 255767520

Gene position: 2486795-2485680 (Counterclockwise)

Preceding gene: 255767521

Following gene: 255767518

Centisome position: 58.99

GC content: 46.24

Gene sequence:

>1116_bases
ATGGTGAATGAAAAACGCCTGCTGGAAGAATTTTTAGAGCTTGTCCAAATTGACTCAGAAACAAAGCACGAAGCTGAAAT
CTGCAAAGTGCTGAAACGAAAATTTTCTGATTTAGGTGTGGACGTTAAAGAAGACGATACAATGGATATCACGGGCCACG
GAGCCGGTAACCTGATTTGCACGCTAAAAGGGACAAAACAAACAGATACGATCTATTTTACATCTCATATGGACACAGTT
GTACCCGGAAACGGCGTAAAACCAGTTGTAGAGAACGGTTACGTCAAAACTGACGGCACGACGATTTTAGGTGCTGATGA
TAAAGCGGGATTGGCTGCAATGTTTGAGGCGATTAAGGTGCTGAAAGAAGAAAATATTGAACACGGCACGATTGAATTCA
TCATCACAGTAGGGGAAGAATCGGGTCTGATCGGAGCGAAGGCGCTTGACCGTTCTATGATCACTGCATCCTACGGCTAT
GCTCTTGATTCAGACGGAAAAGTCGGAAATATCATTGTGGCCGCGCCGACACAGGCGAAGGTAAGAGCGGCAATTTTCGG
GAAAACCGCTCACGCCGGGGTTGAACCTGAAAAAGGCATTTCAGCGATTACAATCGCTAGCAAAGCCATCTCAAAAATGC
CATTGGGCCGTATCGATGAAGAAACAACAGCTAATATCGGCCGGTTTGAAGGCGGCACACAAACAAACATTGTGTGCGAC
GAAGTTCATATTCTTGCTGAAGCGCGCTCTTTAGTTCCTGAGAAAATGGAAGCTCAGGTTCAAAAAATGAAAGCTGCTTT
TGAAGAGGCAGCTGCTGATATGGGCGGACGCGCAGAAGTCGAAATTGAGGTTATGTATCCGGGCTTTAAATATCAAGACG
GCGACCAAGTTGTTGAAATCGCGAAAAAAGCGGCTGCTAAAATCGGACGTCCAAGCGAGCTGCAAACGAGCGGCGGCGGA
AGCGACGCGAACGTAATCGCTGGTCATGGCATCCCGACGGTCAATCTTGCTGTCGGTTATGAGCAAATCCATACAAAAAA
TGAAAAAATGCCGATCGAAGAGCTCGTTAAAACGGCAGAAATGGTTGTTGCCATTATTGAAGAGGCGGCAAAATAA

Upstream 100 bases:

>100_bases
GAAGACTGATTCAAGCTTTTCATATGCTTACACATAAGACAGAAGAGAGACCGAAAAAAAAGCACGGTAACATACCGCTT
TAATATGGAGGGACTTACAA

Downstream 100 bases:

>100_bases
AAAAAGAAAACCCGAGTGCGATTTCCCGCATCGGGTTTTTTTAGCTCCATTTCCTAAGCGGCCAAAATAACACATCCTGT
TTTTTAGCATAATGAAGCAA

Product: deacylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MVNEKRLLEEFLELVQIDSETKHEAEICKVLKRKFSDLGVDVKEDDTMDITGHGAGNLICTLKGTKQTDTIYFTSHMDTV
VPGNGVKPVVENGYVKTDGTTILGADDKAGLAAMFEAIKVLKEENIEHGTIEFIITVGEESGLIGAKALDRSMITASYGY
ALDSDGKVGNIIVAAPTQAKVRAAIFGKTAHAGVEPEKGISAITIASKAISKMPLGRIDEETTANIGRFEGGTQTNIVCD
EVHILAEARSLVPEKMEAQVQKMKAAFEEAAADMGGRAEVEIEVMYPGFKYQDGDQVVEIAKKAAAKIGRPSELQTSGGG
SDANVIAGHGIPTVNLAVGYEQIHTKNEKMPIEELVKTAEMVVAIIEEAAK

Sequences:

>Translated_371_residues
MVNEKRLLEEFLELVQIDSETKHEAEICKVLKRKFSDLGVDVKEDDTMDITGHGAGNLICTLKGTKQTDTIYFTSHMDTV
VPGNGVKPVVENGYVKTDGTTILGADDKAGLAAMFEAIKVLKEENIEHGTIEFIITVGEESGLIGAKALDRSMITASYGY
ALDSDGKVGNIIVAAPTQAKVRAAIFGKTAHAGVEPEKGISAITIASKAISKMPLGRIDEETTANIGRFEGGTQTNIVCD
EVHILAEARSLVPEKMEAQVQKMKAAFEEAAADMGGRAEVEIEVMYPGFKYQDGDQVVEIAKKAAAKIGRPSELQTSGGG
SDANVIAGHGIPTVNLAVGYEQIHTKNEKMPIEELVKTAEMVVAIIEEAAK
>Mature_371_residues
MVNEKRLLEEFLELVQIDSETKHEAEICKVLKRKFSDLGVDVKEDDTMDITGHGAGNLICTLKGTKQTDTIYFTSHMDTV
VPGNGVKPVVENGYVKTDGTTILGADDKAGLAAMFEAIKVLKEENIEHGTIEFIITVGEESGLIGAKALDRSMITASYGY
ALDSDGKVGNIIVAAPTQAKVRAAIFGKTAHAGVEPEKGISAITIASKAISKMPLGRIDEETTANIGRFEGGTQTNIVCD
EVHILAEARSLVPEKMEAQVQKMKAAFEEAAADMGGRAEVEIEVMYPGFKYQDGDQVVEIAKKAAAKIGRPSELQTSGGG
SDANVIAGHGIPTVNLAVGYEQIHTKNEKMPIEELVKTAEMVVAIIEEAAK

Specific function: Could be a peptidase

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family

Homologues:

Organism=Escherichia coli, GI1787372, Length=418, Percent_Identity=26.3157894736842, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YQJE_BACSU (P54542)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   E69963
- RefSeq:   NP_390271.2
- ProteinModelPortal:   P54542
- SMR:   P54542
- MEROPS:   M20.018
- EnsemblBacteria:   EBBACT00000001686
- GeneID:   938687
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23910
- NMPDR:   fig|224308.1.peg.2395
- GenoList:   BSU23910
- GeneTree:   EBGT00050000000947
- HOGENOM:   HBG360563
- PhylomeDB:   P54542
- ProtClustDB:   CLSK873035
- BioCyc:   BSUB:BSU23910-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001261
- InterPro:   IPR010162
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01883

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: 3.4.11.4 [C]

Molecular weight: Translated: 39648; Mature: 39648

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 78-78 ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNEKRLLEEFLELVQIDSETKHEAEICKVLKRKFSDLGVDVKEDDTMDITGHGAGNLIC
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEE
TLKGTKQTDTIYFTSHMDTVVPGNGVKPVVENGYVKTDGTTILGADDKAGLAAMFEAIKV
EEECCCCCCEEEEECCCCEECCCCCCCHHHHCCEEEECCCEEEECCCCCHHHHHHHHHHH
LKEENIEHGTIEFIITVGEESGLIGAKALDRSMITASYGYALDSDGKVGNIIVAAPTQAK
HHHCCCCCCEEEEEEEECCCCCCEEHHHHHHHHHEEECCEEECCCCCCCEEEEECCCCHH
VRAAIFGKTAHAGVEPEKGISAITIASKAISKMPLGRIDEETTANIGRFEGGTQTNIVCD
EEEEEECCHHCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEHH
EVHILAEARSLVPEKMEAQVQKMKAAFEEAAADMGGRAEVEIEVMYPGFKYQDGDQVVEI
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHH
AKKAAAKIGRPSELQTSGGGSDANVIAGHGIPTVNLAVGYEQIHTKNEKMPIEELVKTAE
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCCCCCCHHHHHHHHH
MVVAIIEEAAK
HHHHHHHHHCC
>Mature Secondary Structure
MVNEKRLLEEFLELVQIDSETKHEAEICKVLKRKFSDLGVDVKEDDTMDITGHGAGNLIC
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEEE
TLKGTKQTDTIYFTSHMDTVVPGNGVKPVVENGYVKTDGTTILGADDKAGLAAMFEAIKV
EEECCCCCCEEEEECCCCEECCCCCCCHHHHCCEEEECCCEEEECCCCCHHHHHHHHHHH
LKEENIEHGTIEFIITVGEESGLIGAKALDRSMITASYGYALDSDGKVGNIIVAAPTQAK
HHHCCCCCCEEEEEEEECCCCCCEEHHHHHHHHHEEECCEEECCCCCCCEEEEECCCCHH
VRAAIFGKTAHAGVEPEKGISAITIASKAISKMPLGRIDEETTANIGRFEGGTQTNIVCD
EEEEEECCHHCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEHH
EVHILAEARSLVPEKMEAQVQKMKAAFEEAAADMGGRAEVEIEVMYPGFKYQDGDQVVEI
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHH
AKKAAAKIGRPSELQTSGGGSDANVIAGHGIPTVNLAVGYEQIHTKNEKMPIEELVKTAE
HHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCCCCCCHHHHHHHHH
MVVAIIEEAAK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Aminopeptidases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377