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The map label for this gene is yqjH

Identifier: 255767518

GI number: 255767518

Start: 2482269

End: 2483513

Strand: Reverse

Name: yqjH

Synonym: BSU23870

Alternate gene names: 255767518

Gene position: 2483513-2482269 (Counterclockwise)

Preceding gene: 255767520

Following gene: 50812264

Centisome position: 58.91

GC content: 46.18

Gene sequence:

>1245_bases
ATGCCGGGAAAGAGCCGAATTATTTTCCATATTGATATGAATAGCTTTTATGCGTCGGTTGAAATGGCATATGATCCGGC
TCTCCGGGGAAAGCCGGTCGCAGTTGCCGGAAACGTAAAGGAGCGAAAGGGCATTGTGGTAACATGCAGCTACGAAGCAA
GAGCGCGCGGCGTCAAAACAACGATGCCGGTTTGGCAAGCGAAGCGCCATTGTCCTGAGCTCATCGTTCTTCCGCCGAAC
TTTGACCGCTATAGAAACTCTTCACGAGCGATGTTTACCATTCTTCGTGAATATACTGACCTAGTGGAGCCTGTCTCCAT
CGACGAAGGCTATATGGACATGACCGATACACCATACAGCAGCCGCGCTCTAGAGACGGCAAAAGAAATCCAAAGCAGGC
TGCAAAAAGAGCTGCTGCTCCCGTCAAGCATAGGGATTGCACCGAATAAATTTCTCGCTAAGATGGCGTCTGATATGAAA
AAACCGCTTGGAATTACGATCTTAAGAAAGAGGCAAGTGCCCGATATCCTGTGGCCGCTTCCTGTTGGAGAGATGCACGG
AGTCGGCAAAAAGACAGCGGAAAAACTGAAGGGACTCGGCATCCATACAATCGGAGAGCTAGCTGCAGCTGATGAACACT
CGCTGAAGCGCCTGCTTGGCATCAACGGTCCGCGCCTTAAAAATAAAGCAAATGGCATTCACCATGCGCCGGTTGACCCG
GAGCGCATTTACGAATTTAAAAGCGTCGGCAACTCATCGACACTTTCCCATGATTCCAGTGATGAAGAAGAGCTGTTAGG
CGTTTTTAGAAAGCTTGCCGCTTCTGTGAGTGACCGTTTACAGCGCAAGGAAGTCATGGCTTCGAAGCTTTTTATTATGA
TTAGATATGCGGATTGGAGAACCATCACAAGAAGCACTACGCTGAGAAACCCGATTGATCAAAAAAATGACATTTTGAAA
GAAGCGGAGCACCTCTTTTTTAAACATTGGAATAAAAATCCTGTCAGGCTGCTTGGCATTACCGGAACGGATTTAGTAGA
AAAAGAGCAGGCCTATAAACAGCTCGATTTGTTCAGCTTTAATGAAGATGCGAAGGATGAGCCCATTCAGCAGATGATGG
AAAAGCTGAATAAAAAGTACGGTACCAAACTGATCAGAAAAGGGGCAACGTTAAAAAAAGAGGAGAGCAAAACAAAGGGA
ACAAGCTTTAATAAAGATTTTTTTCAAGATGAAAAGAAAAGCTGA

Upstream 100 bases:

>100_bases
CATTTATCGATTCCACAAACATTGTCATCACATCCATTCAATAGCTTCATTTTACTATATGTACAAGCTGTTGTCTAATG
ACCGAAAAGGAGATTTGTTT

Downstream 100 bases:

>100_bases
ATCGCTTGAAAAAAAGGGTGAATGTATGGTAATTTCTTTATTATAAATATCATTTTATTTTAATTTAAATTTTAAAATGA
TTAAACGTTTGCAGAAGGGA

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV 1

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MPGKSRIIFHIDMNSFYASVEMAYDPALRGKPVAVAGNVKERKGIVVTCSYEARARGVKTTMPVWQAKRHCPELIVLPPN
FDRYRNSSRAMFTILREYTDLVEPVSIDEGYMDMTDTPYSSRALETAKEIQSRLQKELLLPSSIGIAPNKFLAKMASDMK
KPLGITILRKRQVPDILWPLPVGEMHGVGKKTAEKLKGLGIHTIGELAAADEHSLKRLLGINGPRLKNKANGIHHAPVDP
ERIYEFKSVGNSSTLSHDSSDEEELLGVFRKLAASVSDRLQRKEVMASKLFIMIRYADWRTITRSTTLRNPIDQKNDILK
EAEHLFFKHWNKNPVRLLGITGTDLVEKEQAYKQLDLFSFNEDAKDEPIQQMMEKLNKKYGTKLIRKGATLKKEESKTKG
TSFNKDFFQDEKKS

Sequences:

>Translated_414_residues
MPGKSRIIFHIDMNSFYASVEMAYDPALRGKPVAVAGNVKERKGIVVTCSYEARARGVKTTMPVWQAKRHCPELIVLPPN
FDRYRNSSRAMFTILREYTDLVEPVSIDEGYMDMTDTPYSSRALETAKEIQSRLQKELLLPSSIGIAPNKFLAKMASDMK
KPLGITILRKRQVPDILWPLPVGEMHGVGKKTAEKLKGLGIHTIGELAAADEHSLKRLLGINGPRLKNKANGIHHAPVDP
ERIYEFKSVGNSSTLSHDSSDEEELLGVFRKLAASVSDRLQRKEVMASKLFIMIRYADWRTITRSTTLRNPIDQKNDILK
EAEHLFFKHWNKNPVRLLGITGTDLVEKEQAYKQLDLFSFNEDAKDEPIQQMMEKLNKKYGTKLIRKGATLKKEESKTKG
TSFNKDFFQDEKKS
>Mature_413_residues
PGKSRIIFHIDMNSFYASVEMAYDPALRGKPVAVAGNVKERKGIVVTCSYEARARGVKTTMPVWQAKRHCPELIVLPPNF
DRYRNSSRAMFTILREYTDLVEPVSIDEGYMDMTDTPYSSRALETAKEIQSRLQKELLLPSSIGIAPNKFLAKMASDMKK
PLGITILRKRQVPDILWPLPVGEMHGVGKKTAEKLKGLGIHTIGELAAADEHSLKRLLGINGPRLKNKANGIHHAPVDPE
RIYEFKSVGNSSTLSHDSSDEEELLGVFRKLAASVSDRLQRKEVMASKLFIMIRYADWRTITRSTTLRNPIDQKNDILKE
AEHLFFKHWNKNPVRLLGITGTDLVEKEQAYKQLDLFSFNEDAKDEPIQQMMEKLNKKYGTKLIRKGATLKKEESKTKGT
SFNKDFFQDEKKS

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain

Homologues:

Organism=Homo sapiens, GI154350220, Length=307, Percent_Identity=29.9674267100977, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI84043967, Length=414, Percent_Identity=26.0869565217391, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI7706681, Length=415, Percent_Identity=26.0240963855422, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI7705344, Length=111, Percent_Identity=44.1441441441441, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI5729982, Length=157, Percent_Identity=31.8471337579618, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1786425, Length=343, Percent_Identity=34.9854227405248, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1787432, Length=397, Percent_Identity=25.6926952141058, Blast_Score=101, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17537959, Length=370, Percent_Identity=26.2162162162162, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI193205700, Length=422, Percent_Identity=24.6445497630332, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI115534089, Length=160, Percent_Identity=31.875, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI193205702, Length=365, Percent_Identity=22.4657534246575, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324921, Length=209, Percent_Identity=29.1866028708134, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19923006, Length=445, Percent_Identity=26.5168539325843, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21355641, Length=274, Percent_Identity=31.021897810219, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24644984, Length=274, Percent_Identity=31.021897810219, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24668444, Length=510, Percent_Identity=24.7058823529412, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DPO41_BACSU (P54545)

Other databases:

- EMBL:   D84432
- EMBL:   AL009126
- PIR:   H69963
- RefSeq:   NP_390268.2
- ProteinModelPortal:   P54545
- SMR:   P54545
- EnsemblBacteria:   EBBACT00000000054
- GeneID:   938691
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23870
- NMPDR:   fig|224308.1.peg.2392
- GenoList:   BSU23870
- GeneTree:   EBGT00050000001510
- HOGENOM:   HBG734504
- PhylomeDB:   P54545
- ProtClustDB:   PRK01810
- BioCyc:   BSUB:BSU23870-MONOMER
- BRENDA:   2.7.7.7
- GO:   GO:0005737
- HAMAP:   MF_01113
- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880
- Gene3D:   G3DSA:3.30.1490.100
- PANTHER:   PTHR11076

Pfam domain/function: PF00817 IMS; SSF100879 DNA_pol_Y-fam_little_finger

EC number: =2.7.7.7

Molecular weight: Translated: 47007; Mature: 46876

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50173 UMUC

Important sites: ACT_SITE 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGKSRIIFHIDMNSFYASVEMAYDPALRGKPVAVAGNVKERKGIVVTCSYEARARGVKT
CCCCCEEEEEEECCCHHHHEEEEECCCCCCCCEEEECCCHHCCCEEEEEECHHHHCCCCC
TMPVWQAKRHCPELIVLPPNFDRYRNSSRAMFTILREYTDLVEPVSIDEGYMDMTDTPYS
CCHHHHHHHCCCCEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SRALETAKEIQSRLQKELLLPSSIGIAPNKFLAKMASDMKKPLGITILRKRQVPDILWPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
PVGEMHGVGKKTAEKLKGLGIHTIGELAAADEHSLKRLLGINGPRLKNKANGIHHAPVDP
CCHHHCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
ERIYEFKSVGNSSTLSHDSSDEEELLGVFRKLAASVSDRLQRKEVMASKLFIMIRYADWR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCH
TITRSTTLRNPIDQKNDILKEAEHLFFKHWNKNPVRLLGITGTDLVEKEQAYKQLDLFSF
HHHHCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCC
NEDAKDEPIQQMMEKLNKKYGTKLIRKGATLKKEESKTKGTSFNKDFFQDEKKS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
PGKSRIIFHIDMNSFYASVEMAYDPALRGKPVAVAGNVKERKGIVVTCSYEARARGVKT
CCCCEEEEEEECCCHHHHEEEEECCCCCCCCEEEECCCHHCCCEEEEEECHHHHCCCCC
TMPVWQAKRHCPELIVLPPNFDRYRNSSRAMFTILREYTDLVEPVSIDEGYMDMTDTPYS
CCHHHHHHHCCCCEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SRALETAKEIQSRLQKELLLPSSIGIAPNKFLAKMASDMKKPLGITILRKRQVPDILWPL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
PVGEMHGVGKKTAEKLKGLGIHTIGELAAADEHSLKRLLGINGPRLKNKANGIHHAPVDP
CCHHHCCCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCH
ERIYEFKSVGNSSTLSHDSSDEEELLGVFRKLAASVSDRLQRKEVMASKLFIMIRYADWR
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCH
TITRSTTLRNPIDQKNDILKEAEHLFFKHWNKNPVRLLGITGTDLVEKEQAYKQLDLFSF
HHHHCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCC
NEDAKDEPIQQMMEKLNKKYGTKLIRKGATLKKEESKTKGTSFNKDFFQDEKKS
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377