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The map label for this gene is aroF

Identifier: 255767489

GI number: 255767489

Start: 2379100

End: 2380272

Strand: Reverse

Name: aroF

Synonym: BSU22710

Alternate gene names: 255767489

Gene position: 2380272-2379100 (Counterclockwise)

Preceding gene: 50812262

Following gene: 16079327

Centisome position: 56.46

GC content: 48.42

Gene sequence:

>1173_bases
ATGAGATATTTAACAGCCGGAGAATCACACGGCCCCCAACTGACAACCATTATTGAGGGTGTACCTGCCGGGCTTTACAT
AACGGAGGAAGATATCAATTTTGAGCTTGCCAGACGCCAAAAAGGACACGGCCGCGGCCGCCGCATGCAGATCGAAAAAG
ACCAGGCCAAAATTATGAGCGGGGTGCGCCATGCACGTACACTCGGTTCGCCAATTGCTCTCGTAGTTGAAAATAACGAT
TGGAAGCATTGGACAAAAATCATGGGCGCCGCTCCGATTACAGAAGATGAAGAAAAGGAAATGAAGCGCCAGATTTCCAG
ACCGAGACCTGGGCACGCTGACTTAAACGGTGCAATTAAATATAATCATCGTGATATGAGAAATGTGCTTGAGCGTTCTT
CAGCAAGGGAAACAACTGTCAGAGTGGCGGCAGGGGCTGTAGCTAAAAAGATTCTTTCTGAGCTTGGCATTAAGGTGGCG
GGCCACGTGTTGCAAATTGGCGCTGTTAAAGCTGAAAAAACAGAGTATACGTCAATTGAAGACTTGCAGCGTGTAACGGA
AGAGTCTCCTGTCAGATGCTACGATGAAGAGGCAGGCAAAAAAATGATGGCTGCCATTGATGAAGCAAAGGCAAACGGAG
ATTCCATCGGCGGAATAGTAGAAGTCATTGTCGAGGGAATGCCGGTAGGCGTGGGCAGCTATGTCCATTATGACCGCAAG
CTGGATAGCAAGCTTGCTGCCGCTGTTCTGTCAATTAACGCATTTAAAGGTGTGGAATTCGGCATCGGATTTGAGGCAGC
AGGGCGAAATGGAAGCGAAGTCCATGACGAGATCATTTGGGACGAGGAAAAAGGATACACCCGTGCTACTAACAGACTTG
GCGGACTGGAAGGCGGCATGACGACAGGGATGCCGATTGTTGTCCGCGGAGTCATGAAGCCGATTCCGACTTTGTATAAA
CCGCTGAAAAGTGTCGATATTGAAACGAAAGAACCGTTTTCTGCCAGCATTGAACGTTCAGACAGCTGTGCTGTTCCTGC
GGCAAGTGTAGTCGCTGAAGCGGCTGTCGCTTGGGAAATTGCGAACGCAGTCGTTGAACAATTCGGATTAGATCAAATTG
ACCGCATTAGAGAAAATGTGGAGAATATGAGAAAACTGTCGAGGGAATTTTAA

Upstream 100 bases:

>100_bases
ATACATTCTTTTATCAAAAAAGATAGACTGACTTTTTGCTGAAAAATATGTTATAGTAGTCTTACTTAAAAGCGTTAATG
CGATGAAGGGAGAAGAAGTC

Downstream 100 bases:

>100_bases
TGAAGACACTGCATGTTCAAACTGCTTCCTCGTCATATCCTGTTTTTATCGGACAAGGTATCAGAAAAAAGGCTTGCGAA
CTATTAACCTCTTTAAACAG

Product: chorismate synthase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate phospholyase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MRYLTAGESHGPQLTTIIEGVPAGLYITEEDINFELARRQKGHGRGRRMQIEKDQAKIMSGVRHARTLGSPIALVVENND
WKHWTKIMGAAPITEDEEKEMKRQISRPRPGHADLNGAIKYNHRDMRNVLERSSARETTVRVAAGAVAKKILSELGIKVA
GHVLQIGAVKAEKTEYTSIEDLQRVTEESPVRCYDEEAGKKMMAAIDEAKANGDSIGGIVEVIVEGMPVGVGSYVHYDRK
LDSKLAAAVLSINAFKGVEFGIGFEAAGRNGSEVHDEIIWDEEKGYTRATNRLGGLEGGMTTGMPIVVRGVMKPIPTLYK
PLKSVDIETKEPFSASIERSDSCAVPAASVVAEAAVAWEIANAVVEQFGLDQIDRIRENVENMRKLSREF

Sequences:

>Translated_390_residues
MRYLTAGESHGPQLTTIIEGVPAGLYITEEDINFELARRQKGHGRGRRMQIEKDQAKIMSGVRHARTLGSPIALVVENND
WKHWTKIMGAAPITEDEEKEMKRQISRPRPGHADLNGAIKYNHRDMRNVLERSSARETTVRVAAGAVAKKILSELGIKVA
GHVLQIGAVKAEKTEYTSIEDLQRVTEESPVRCYDEEAGKKMMAAIDEAKANGDSIGGIVEVIVEGMPVGVGSYVHYDRK
LDSKLAAAVLSINAFKGVEFGIGFEAAGRNGSEVHDEIIWDEEKGYTRATNRLGGLEGGMTTGMPIVVRGVMKPIPTLYK
PLKSVDIETKEPFSASIERSDSCAVPAASVVAEAAVAWEIANAVVEQFGLDQIDRIRENVENMRKLSREF
>Mature_390_residues
MRYLTAGESHGPQLTTIIEGVPAGLYITEEDINFELARRQKGHGRGRRMQIEKDQAKIMSGVRHARTLGSPIALVVENND
WKHWTKIMGAAPITEDEEKEMKRQISRPRPGHADLNGAIKYNHRDMRNVLERSSARETTVRVAAGAVAKKILSELGIKVA
GHVLQIGAVKAEKTEYTSIEDLQRVTEESPVRCYDEEAGKKMMAAIDEAKANGDSIGGIVEVIVEGMPVGVGSYVHYDRK
LDSKLAAAVLSINAFKGVEFGIGFEAAGRNGSEVHDEIIWDEEKGYTRATNRLGGLEGGMTTGMPIVVRGVMKPIPTLYK
PLKSVDIETKEPFSASIERSDSCAVPAASVVAEAAVAWEIANAVVEQFGLDQIDRIRENVENMRKLSREF

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; seventh step. [C]

COG id: COG0082

COG function: function code E; Chorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788669, Length=368, Percent_Identity=35.8695652173913, Blast_Score=176, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321290, Length=376, Percent_Identity=32.9787234042553, Blast_Score=185, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000453
- InterPro:   IPR020541 [H]

Pfam domain/function: PF01264 Chorismate_synt [H]

EC number: =4.2.3.5 [H]

Molecular weight: Translated: 42806; Mature: 42806

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00787 CHORISMATE_SYNTHASE_1 ; PS00788 CHORISMATE_SYNTHASE_2 ; PS00789 CHORISMATE_SYNTHASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLTAGESHGPQLTTIIEGVPAGLYITEEDINFELARRQKGHGRGRRMQIEKDQAKIMS
CCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHH
GVRHARTLGSPIALVVENNDWKHWTKIMGAAPITEDEEKEMKRQISRPRPGHADLNGAIK
HHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEE
YNHRDMRNVLERSSARETTVRVAAGAVAKKILSELGIKVAGHVLQIGAVKAEKTEYTSIE
ECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCEECCCCCCCCHH
DLQRVTEESPVRCYDEEAGKKMMAAIDEAKANGDSIGGIVEVIVEGMPVGVGSYVHYDRK
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
LDSKLAAAVLSINAFKGVEFGIGFEAAGRNGSEVHDEIIWDEEKGYTRATNRLGGLEGGM
HHHHHHHHHHHHHHHCCCEECCCEECCCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCCCC
TTGMPIVVRGVMKPIPTLYKPLKSVDIETKEPFSASIERSDSCAVPAASVVAEAAVAWEI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
ANAVVEQFGLDQIDRIRENVENMRKLSREF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRYLTAGESHGPQLTTIIEGVPAGLYITEEDINFELARRQKGHGRGRRMQIEKDQAKIMS
CCCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHH
GVRHARTLGSPIALVVENNDWKHWTKIMGAAPITEDEEKEMKRQISRPRPGHADLNGAIK
HHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEE
YNHRDMRNVLERSSARETTVRVAAGAVAKKILSELGIKVAGHVLQIGAVKAEKTEYTSIE
ECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCEECCCCCCCCHH
DLQRVTEESPVRCYDEEAGKKMMAAIDEAKANGDSIGGIVEVIVEGMPVGVGSYVHYDRK
HHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
LDSKLAAAVLSINAFKGVEFGIGFEAAGRNGSEVHDEIIWDEEKGYTRATNRLGGLEGGM
HHHHHHHHHHHHHHHCCCEECCCEECCCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCCCC
TTGMPIVVRGVMKPIPTLYKPLKSVDIETKEPFSASIERSDSCAVPAASVVAEAAVAWEI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHH
ANAVVEQFGLDQIDRIRENVENMRKLSREF
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA