Click here to switch to the map view.

The map label for this gene is cheR

Identifier: 50812262

GI number: 50812262

Start: 2380347

End: 2381117

Strand: Reverse

Name: cheR

Synonym: BSU22720

Alternate gene names: 50812262

Gene position: 2381117-2380347 (Counterclockwise)

Preceding gene: 16079330

Following gene: 255767489

Centisome position: 56.48

GC content: 39.3

Gene sequence:

>771_bases
ATGGATACATACAGCGTATTTACAACGAAATGGAAACAATTAACCGGAGTCGATTTAACACTATATAAAGAAGCACAAAT
GAAGAGAAGGCTAACGTCACTTTATGAGAAAAAGGGGTTCCAAAGCTTTAAGGACTTTGCTGCGGCATTGGAAAAGGATC
AAGCTCTCTTAAATGAAACATTGGACAGAATGACGATCAATGTTTCAGAATTTTATCGTAATTATAAAAGATGGGAAGTT
CTTGAGACTGCAATTCTGCCGTTAATCAAGACCTCTAGGCCTTTAAAAATTTGGAGTGCCGCCTGCTCAACGGGGGAGGA
GCCATACACGCTTGCCATGCTTTTGGACCAGCAAAAAGGTCTTCCGGGCTATCAGATTTTAGCGACGGATATTGATGAAA
AAGCATTGGAAAAAGCGAAAAAAGGCGTTTATCAGGAGCGGTCTTTACAGGAAGTGCCGCTATCTGTGAAAGATCGTTAT
TTTACGCAAAACGCAAATAGAAGTTATGAAGTGAAAACGGAAATCAAAAAGAATATTACATTCAAAAAGCACAATTTGCT
GGCGGATCGTTATGAACAAGACTTTGACTTAATCGTGTGCCGCAATGTATTTATTTATTTTACAGAAAGCGCGAAGGAAG
AGCTGTATTTAAAAATGGCACATAGCTTGAAGAAGAATGGAGTTCTTTTTGTCGGCAGCACCGAGCAAATCTTCAATCCC
GAGAAATTTGGCCTAGTCCCAGCCGATACATTCTTTTATCAAAAAAGATAG

Upstream 100 bases:

>100_bases
AAAATCAGCTGAAAAGCTGATTTTTTTATTGAAACAATACAATTTCCTGATAAAACAAGCTATACTAGGACAAAAAGACA
GCGAGACTGGGAGAGTAGAG

Downstream 100 bases:

>100_bases
ACTGACTTTTTGCTGAAAAATATGTTATAGTAGTCTTACTTAAAAGCGTTAATGCGATGAAGGGAGAAGAAGTCATGAGA
TATTTAACAGCCGGAGAATC

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MDTYSVFTTKWKQLTGVDLTLYKEAQMKRRLTSLYEKKGFQSFKDFAAALEKDQALLNETLDRMTINVSEFYRNYKRWEV
LETAILPLIKTSRPLKIWSAACSTGEEPYTLAMLLDQQKGLPGYQILATDIDEKALEKAKKGVYQERSLQEVPLSVKDRY
FTQNANRSYEVKTEIKKNITFKKHNLLADRYEQDFDLIVCRNVFIYFTESAKEELYLKMAHSLKKNGVLFVGSTEQIFNP
EKFGLVPADTFFYQKR

Sequences:

>Translated_256_residues
MDTYSVFTTKWKQLTGVDLTLYKEAQMKRRLTSLYEKKGFQSFKDFAAALEKDQALLNETLDRMTINVSEFYRNYKRWEV
LETAILPLIKTSRPLKIWSAACSTGEEPYTLAMLLDQQKGLPGYQILATDIDEKALEKAKKGVYQERSLQEVPLSVKDRY
FTQNANRSYEVKTEIKKNITFKKHNLLADRYEQDFDLIVCRNVFIYFTESAKEELYLKMAHSLKKNGVLFVGSTEQIFNP
EKFGLVPADTFFYQKR
>Mature_256_residues
MDTYSVFTTKWKQLTGVDLTLYKEAQMKRRLTSLYEKKGFQSFKDFAAALEKDQALLNETLDRMTINVSEFYRNYKRWEV
LETAILPLIKTSRPLKIWSAACSTGEEPYTLAMLLDQQKGLPGYQILATDIDEKALEKAKKGVYQERSLQEVPLSVKDRY
FTQNANRSYEVKTEIKKNITFKKHNLLADRYEQDFDLIVCRNVFIYFTESAKEELYLKMAHSLKKNGVLFVGSTEQIFNP
EKFGLVPADTFFYQKR

Specific function: Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP. CheR is responsible for the chemotactic adaptation to repellents

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain

Homologues:

Organism=Escherichia coli, GI1788193, Length=243, Percent_Identity=31.6872427983539, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHER_BACSU (P31105)

Other databases:

- EMBL:   X73681
- EMBL:   M80245
- EMBL:   AL009126
- PIR:   A69599
- RefSeq:   NP_390153.2
- ProteinModelPortal:   P31105
- SMR:   P31105
- EnsemblBacteria:   EBBACT00000000735
- GeneID:   939003
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU22720
- GenoList:   BSU22720
- GeneTree:   EBGT00050000000368
- HOGENOM:   HBG661948
- OMA:   RIRRILI
- PhylomeDB:   P31105
- ProtClustDB:   CLSK928145
- BioCyc:   BSUB:BSU22720-MONOMER
- BRENDA:   2.1.1.80
- GO:   GO:0006935
- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- Gene3D:   G3DSA:1.10.155.10
- PRINTS:   PR00996
- SMART:   SM00138

Pfam domain/function: PF01739 CheR; PF03705 CheR_N; SSF47757 CheR_mtfrase

EC number: =2.1.1.80

Molecular weight: Translated: 29953; Mature: 29953

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50123 CHER

Important sites: BINDING 67-67 BINDING 69-69 BINDING 73-73 BINDING 107-107 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTYSVFTTKWKQLTGVDLTLYKEAQMKRRLTSLYEKKGFQSFKDFAAALEKDQALLNET
CCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
LDRMTINVSEFYRNYKRWEVLETAILPLIKTSRPLKIWSAACSTGEEPYTLAMLLDQQKG
HHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEEHHCCC
LPGYQILATDIDEKALEKAKKGVYQERSLQEVPLSVKDRYFTQNANRSYEVKTEIKKNIT
CCCEEEEEECCCHHHHHHHHHCHHHHCCHHHCCCCHHHHHHHCCCCCCEEEHHHHHHCCC
FKKHNLLADRYEQDFDLIVCRNVFIYFTESAKEELYLKMAHSLKKNGVLFVGSTEQIFNP
HHHHHHHHHHHCCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHHCCEEEEECCHHHCCC
EKFGLVPADTFFYQKR
HHCCCCCCHHHHEECC
>Mature Secondary Structure
MDTYSVFTTKWKQLTGVDLTLYKEAQMKRRLTSLYEKKGFQSFKDFAAALEKDQALLNET
CCCCHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
LDRMTINVSEFYRNYKRWEVLETAILPLIKTSRPLKIWSAACSTGEEPYTLAMLLDQQKG
HHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEEHHCCC
LPGYQILATDIDEKALEKAKKGVYQERSLQEVPLSVKDRYFTQNANRSYEVKTEIKKNIT
CCCEEEEEECCCHHHHHHHHHCHHHHCCHHHCCCCHHHHHHHCCCCCCEEEHHHHHHCCC
FKKHNLLADRYEQDFDLIVCRNVFIYFTESAKEELYLKMAHSLKKNGVLFVGSTEQIFNP
HHHHHHHHHHHCCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHHCCEEEEECCHHHCCC
EKFGLVPADTFFYQKR
HHCCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8244966; 9384377; 10568751