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The map label for this gene is dapG

Identifier: 255767377

GI number: 255767377

Start: 1747123

End: 1748337

Strand: Direct

Name: dapG

Synonym: BSU16760

Alternate gene names: 255767377

Gene position: 1747123-1748337 (Clockwise)

Preceding gene: 16078738

Following gene: 16078740

Centisome position: 41.44

GC content: 47.9

Gene sequence:

>1215_bases
GTGAAGATAATTGTTCAAAAGTTCGGCGGAACATCAGTAAAAGATGATAAAGGCCGCAAACTGGCTTTAGGGCATATTAA
AGAAGCAATCAGTGAAGGATATAAGGTTGTCGTTGTCGTTTCAGCGATGGGCCGAAAAGGGGACCCGTATGCAACAGATT
CTTTGCTCGGGCTGCTTTACGGCGATCAGTCAGCGATTTCTCCTAGGGAGCAGGATTTGCTTTTATCTTGCGGAGAGACT
ATTTCCTCTGTCGTCTTTACAAGCATGCTGCTTGATAACGGTGTGAAAGCAGCTGCGCTGACAGGGGCTCAGGCTGGCTT
TTTAACGAATGATCAGCATACTAATGCGAAAATCATTGAAATGAAACCGGAGCGTTTATTCAGTGTGCTCGCCAATCATG
ATGCGGTAGTCGTCGCAGGCTTCCAGGGCGCAACTGAAAAGGGTGATACAACAACAATCGGCAGAGGGGGCAGCGATACG
TCTGCAGCCGCACTCGGAGCAGCTGTTGATGCGGAATACATCGATATTTTCACCGATGTAGAAGGCGTGATGACTGCTGA
CCCAAGAGTGGTAGAAAATGCAAAACCGCTTCCTGTTGTGACGTATACGGAAATCTGCAACCTGGCGTATCAGGGGGCCA
AGGTCATTTCTCCGCGTGCTGTGGAAATCGCAATGCAGGCCAAGGTTCCGATCCGCGTCCGTTCGACTTATTCGAATGAT
AAAGGCACATTGGTGACATCACATCACTCTTCCAAGGTCGGCAGTGATGTTTTTGAAAGGCTGATTACGGGAATCGCCCA
TGTCAAAGATGTCACGCAGTTCAAAGTGCCTGCGAAAATCGGCCAGTATAACGTTCAGACTGAAGTGTTCAAGGCTATGG
CAAACGCTGGAATCAGTGTCGATTTCTTTAACATTACACCTAGTGAAATCGTCTATACGGTAGCCGGAAATAAAACAGAA
ACAGCTCAGCGTATTTTAATGGATATGGGCTATGATCCAATGGTGACGAGAAACTGCGCGAAGGTATCGGCGGTCGGCGC
GGGCATTATGGGTGTTCCCGGTGTTACGTCCAAAATTGTATCGGCTCTTTCAGAAAAAGAAATTCCGATCCTTCAGTCGG
CTGACAGCCATACGACGATATGGGTGCTCGTCCATGAAGCGGATATGGTTCCTGCTGTAAACGCTTTGCATGAAGTGTTT
GAACTTTCGAAGTAA

Upstream 100 bases:

>100_bases
CTCGTATAAAAGAGAGAATTTTCTAAAGACGAATAGAAGAGAGTAAGGCGCTATCAGCCTGCTCTCTTCTGTTACGTCCG
AATAATTTGGAGTGAAAACA

Downstream 100 bases:

>100_bases
ATGTGATTGAATCATGATGAGGTGAATAAAATGAATTTCGGAAATGTGTCTACAGCGATGATTACACCCTTTGACAATAA
AGGGAACGTAGACTTTCAAA

Product: aspartate kinase I

Products: NA

Alternate protein names: Aspartate kinase 1; Aspartokinase I

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMGRKGDPYATDSLLGLLYGDQSAISPREQDLLLSCGET
ISSVVFTSMLLDNGVKAAALTGAQAGFLTNDQHTNAKIIEMKPERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDT
SAAALGAAVDAEYIDIFTDVEGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISPRAVEIAMQAKVPIRVRSTYSND
KGTLVTSHHSSKVGSDVFERLITGIAHVKDVTQFKVPAKIGQYNVQTEVFKAMANAGISVDFFNITPSEIVYTVAGNKTE
TAQRILMDMGYDPMVTRNCAKVSAVGAGIMGVPGVTSKIVSALSEKEIPILQSADSHTTIWVLVHEADMVPAVNALHEVF
ELSK

Sequences:

>Translated_404_residues
MKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMGRKGDPYATDSLLGLLYGDQSAISPREQDLLLSCGET
ISSVVFTSMLLDNGVKAAALTGAQAGFLTNDQHTNAKIIEMKPERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDT
SAAALGAAVDAEYIDIFTDVEGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISPRAVEIAMQAKVPIRVRSTYSND
KGTLVTSHHSSKVGSDVFERLITGIAHVKDVTQFKVPAKIGQYNVQTEVFKAMANAGISVDFFNITPSEIVYTVAGNKTE
TAQRILMDMGYDPMVTRNCAKVSAVGAGIMGVPGVTSKIVSALSEKEIPILQSADSHTTIWVLVHEADMVPAVNALHEVF
ELSK
>Mature_404_residues
MKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMGRKGDPYATDSLLGLLYGDQSAISPREQDLLLSCGET
ISSVVFTSMLLDNGVKAAALTGAQAGFLTNDQHTNAKIIEMKPERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDT
SAAALGAAVDAEYIDIFTDVEGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISPRAVEIAMQAKVPIRVRSTYSND
KGTLVTSHHSSKVGSDVFERLITGIAHVKDVTQFKVPAKIGQYNVQTEVFKAMANAGISVDFFNITPSEIVYTVAGNKTE
TAQRILMDMGYDPMVTRNCAKVSAVGAGIMGVPGVTSKIVSALSEKEIPILQSADSHTTIWVLVHEADMVPAVNALHEVF
ELSK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartokinase family

Homologues:

Organism=Escherichia coli, GI1786183, Length=286, Percent_Identity=32.8671328671329, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790455, Length=358, Percent_Identity=28.2122905027933, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790376, Length=338, Percent_Identity=24.8520710059172, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320893, Length=372, Percent_Identity=25.5376344086022, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AK1_BACSU (Q04795)

Other databases:

- EMBL:   L08471
- EMBL:   AL009126
- PIR:   C46665
- RefSeq:   NP_389558.2
- ProteinModelPortal:   Q04795
- SMR:   Q04795
- EnsemblBacteria:   EBBACT00000000346
- GeneID:   936710
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16760
- NMPDR:   fig|224308.1.peg.1679
- GenoList:   BSU16760
- GeneTree:   EBGT00050000001782
- HOGENOM:   HBG724395
- ProtClustDB:   PRK08210
- BioCyc:   BSUB:BSU16760-MONOMER
- BRENDA:   2.7.2.4
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042
- Gene3D:   G3DSA:3.40.1160.10
- TIGRFAMs:   TIGR00656
- TIGRFAMs:   TIGR00657

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.4

Molecular weight: Translated: 42949; Mature: 42949

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMGRKGDPYATDSLLGLLY
CEEEEEECCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHEEE
GDQSAISPREQDLLLSCGETISSVVFTSMLLDNGVKAAALTGAQAGFLTNDQHTNAKIIE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEE
MKPERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDTSAAALGAAVDAEYIDIFTDV
ECHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHCCHHHHHHHHCC
EGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISPRAVEIAMQAKVPIRVRSTYSND
CCEEECCCHHHCCCCCCCEEEHHHHHHHHHCCCEECCCCEEEEEEECCCCEEEEECCCCC
KGTLVTSHHSSKVGSDVFERLITGIAHVKDVTQFKVPAKIGQYNVQTEVFKAMANAGISV
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCHHHHHHHHHHHCCCEE
DFFNITPSEIVYTVAGNKTETAQRILMDMGYDPMVTRNCAKVSAVGAGIMGVPGVTSKIV
EEEECCHHHEEEEECCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHCCCCCCCCCHHHHHH
SALSEKEIPILQSADSHTTIWVLVHEADMVPAVNALHEVFELSK
HHHHHCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMGRKGDPYATDSLLGLLY
CEEEEEECCCCCCCCCCCCEEHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHEEE
GDQSAISPREQDLLLSCGETISSVVFTSMLLDNGVKAAALTGAQAGFLTNDQHTNAKIIE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCEEEEE
MKPERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDTSAAALGAAVDAEYIDIFTDV
ECHHHHHHHHHCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHCCHHHHHHHHCC
EGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISPRAVEIAMQAKVPIRVRSTYSND
CCEEECCCHHHCCCCCCCEEEHHHHHHHHHCCCEECCCCEEEEEEECCCCEEEEECCCCC
KGTLVTSHHSSKVGSDVFERLITGIAHVKDVTQFKVPAKIGQYNVQTEVFKAMANAGISV
CCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHCCCCHHHHHHHHHHHCCCEE
DFFNITPSEIVYTVAGNKTETAQRILMDMGYDPMVTRNCAKVSAVGAGIMGVPGVTSKIV
EEEECCHHHEEEEECCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHCCCCCCCCCHHHHHH
SALSEKEIPILQSADSHTTIWVLVHEADMVPAVNALHEVFELSK
HHHHHCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8098035; 9384377