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The map label for this gene is dapA

Identifier: 16078740

GI number: 16078740

Start: 1748368

End: 1749240

Strand: Direct

Name: dapA

Synonym: BSU16770

Alternate gene names: 16078740

Gene position: 1748368-1749240 (Clockwise)

Preceding gene: 255767377

Following gene: 255767378

Centisome position: 41.47

GC content: 44.56

Gene sequence:

>873_bases
ATGAATTTCGGAAATGTGTCTACAGCGATGATTACACCCTTTGACAATAAAGGGAACGTAGACTTTCAAAAACTGTCTAC
ACTGATTGATTACTTGTTGAAAAACGGAACGGATTCTTTAGTAGTAGCGGGAACAACTGGAGAATCTCCGACCCTTTCAA
CTGAAGAAAAAATTGCGCTTTTTGAATATACGGTAAAAGAAGTAAACGGACGGGTGCCCGTTATCGCTGGTACTGGGAGC
AACAACACGAAAGATTCCATTAAGCTGACAAAAAAAGCTGAGGAAGCGGGCGTGGACGCTGTTATGCTTGTTACCCCGTA
TTACAATAAACCTTCTCAAGAAGGAATGTACCAGCATTTTAAAGCAATTGCGGCAGAGACATCTCTTCCGGTTATGCTCT
ATAATGTTCCTGGCCGTACGGTTGCTTCTCTTGCTCCTGAAACGACGATCCGTTTGGCGGCAGACATTCCGAATGTGGTT
GCGATTAAAGAAGCGAGCGGAGACCTCGAAGCGATTACAAAAATTATTGCTGAAACACCTGAAGACTTCTATGTCTATTC
AGGGGATGATGCTCTAACGCTTCCAATTCTTTCAGTTGGAGGTAGAGGAGTTGTGTCAGTGGCGAGCCATATTGCAGGCA
CTGATATGCAGCAAATGATCAAAAATTATACGAATGGGCAAACAGCTAATGCGGCACTGATTCATCAGAAACTGCTGCCG
ATTATGAAGGAACTGTTTAAAGCGCCTAATCCTGCTCCAGTCAAAACAGCGCTTCAGCTGAGAGGTCTTGATGTCGGTTC
TGTGCGTCTGCCTCTAGTCCCATTAACTGAGGATGAACGTCTGAGCTTAAGCAGCACGATCAGCGAACTGTAA

Upstream 100 bases:

>100_bases
GCTCGTCCATGAAGCGGATATGGTTCCTGCTGTAAACGCTTTGCATGAAGTGTTTGAACTTTCGAAGTAAATGTGATTGA
ATCATGATGAGGTGAATAAA

Downstream 100 bases:

>100_bases
GAAAATTTCATACAGTGAAACAAACGCGGTCATTCTCACATTCAGCTGAGTTTGACCGTTTCTTTTACATATTGGTTTTC
CCTAAACCTTCTGCTTTATC

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS; Vegetative protein 81; VEG81

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNFGNVSTAMITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAGTTGESPTLSTEEKIALFEYTVKEVNGRVPVIAGTGS
NNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQHFKAIAAETSLPVMLYNVPGRTVASLAPETTIRLAADIPNVV
AIKEASGDLEAITKIIAETPEDFYVYSGDDALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYTNGQTANAALIHQKLLP
IMKELFKAPNPAPVKTALQLRGLDVGSVRLPLVPLTEDERLSLSSTISEL

Sequences:

>Translated_290_residues
MNFGNVSTAMITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAGTTGESPTLSTEEKIALFEYTVKEVNGRVPVIAGTGS
NNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQHFKAIAAETSLPVMLYNVPGRTVASLAPETTIRLAADIPNVV
AIKEASGDLEAITKIIAETPEDFYVYSGDDALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYTNGQTANAALIHQKLLP
IMKELFKAPNPAPVKTALQLRGLDVGSVRLPLVPLTEDERLSLSSTISEL
>Mature_290_residues
MNFGNVSTAMITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAGTTGESPTLSTEEKIALFEYTVKEVNGRVPVIAGTGS
NNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQHFKAIAAETSLPVMLYNVPGRTVASLAPETTIRLAADIPNVV
AIKEASGDLEAITKIIAETPEDFYVYSGDDALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYTNGQTANAALIHQKLLP
IMKELFKAPNPAPVKTALQLRGLDVGSVRLPLVPLTEDERLSLSSTISEL

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=283, Percent_Identity=26.8551236749117, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI13540533, Length=274, Percent_Identity=26.2773722627737, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1788823, Length=282, Percent_Identity=45.7446808510638, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1786463, Length=275, Percent_Identity=33.0909090909091, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI87082415, Length=274, Percent_Identity=31.3868613138686, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1789620, Length=280, Percent_Identity=31.0714285714286, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_BACSU (Q04796)

Other databases:

- EMBL:   L08471
- EMBL:   AL009126
- PIR:   E46665
- RefSeq:   NP_389559.1
- ProteinModelPortal:   Q04796
- SMR:   Q04796
- EnsemblBacteria:   EBBACT00000002157
- GeneID:   939657
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU16770
- NMPDR:   fig|224308.1.peg.1680
- GenoList:   BSU16770
- GeneTree:   EBGT00050000000637
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- PhylomeDB:   Q04796
- ProtClustDB:   PRK03170
- BioCyc:   BSUB:BSU16770-MONOMER
- BRENDA:   4.2.1.52
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31042; Mature: 31042

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 163-163 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFGNVSTAMITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAGTTGESPTLSTEEKIAL
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEH
FEYTVKEVNGRVPVIAGTGSNNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQHF
HHHHHHHHCCCEEEEEECCCCCCCCCEEEEHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
KAIAAETSLPVMLYNVPGRTVASLAPETTIRLAADIPNVVAIKEASGDLEAITKIIAETP
HHHHHCCCCCEEEEECCCCHHHHCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHCCC
EDFYVYSGDDALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYTNGQTANAALIHQKLLP
CCEEEECCCCEEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
IMKELFKAPNPAPVKTALQLRGLDVGSVRLPLVPLTEDERLSLSSTISEL
HHHHHHCCCCCCCHHHHHEEECCCCCCEEEEEEECCCCCCCHHHHHHHCC
>Mature Secondary Structure
MNFGNVSTAMITPFDNKGNVDFQKLSTLIDYLLKNGTDSLVVAGTTGESPTLSTEEKIAL
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEH
FEYTVKEVNGRVPVIAGTGSNNTKDSIKLTKKAEEAGVDAVMLVTPYYNKPSQEGMYQHF
HHHHHHHHCCCEEEEEECCCCCCCCCEEEEHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
KAIAAETSLPVMLYNVPGRTVASLAPETTIRLAADIPNVVAIKEASGDLEAITKIIAETP
HHHHHCCCCCEEEEECCCCHHHHCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHCCC
EDFYVYSGDDALTLPILSVGGRGVVSVASHIAGTDMQQMIKNYTNGQTANAALIHQKLLP
CCEEEECCCCEEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
IMKELFKAPNPAPVKTALQLRGLDVGSVRLPLVPLTEDERLSLSSTISEL
HHHHHHCCCCCCCHHHHHEEECCCCCCEEEEEEECCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8098035; 9384377; 9298659