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The map label for this gene is pit

Identifier: 255767297

GI number: 255767297

Start: 1349468

End: 1350469

Strand: Reverse

Name: pit

Synonym: BSU12840

Alternate gene names: 255767297

Gene position: 1350469-1349468 (Counterclockwise)

Preceding gene: 16078350

Following gene: 16078348

Centisome position: 32.03

GC content: 45.11

Gene sequence:

>1002_bases
ATGGATATTCTATTAATATTAACGATACTAATAGTCATTTGTGCTCTGGCGTTTGATTTCATCAACGGGTTTCATGATAC
GGCAAACGCAATTGCGACTTCGGTATCAACAAAAGCGTTAAAACCGCGCCATGCCATTATATTGGCTGCTGTGATGAACT
TTGTCGGAGCAATGACGTTTACAGGTGTTGCCAAAACCATTACGAAAGACATCGTAGATCCTTATACGCTGGAAAATGGC
TCTGTTGTCATTTTGGCTGCGCTACTCGCTGCAATTGCCTGGAACCTTATCACATGGTACTACGGTATCCCAAGCAGCTC
ATCCCATGCCATCATCGGCGCTATTGCCGGTGCAGCCATTGCGGCAGCCGGATTTGCAGCGTTAAACTATAAGGGCTTCA
TTAAAATCATTGAAGCACTGATTCTATCACCAATTATTGCCTTTGTCCTTGGTTTTATTCTTTATAGCATCGTTAAGCTG
ATTTTCAAAGACAGCAACTTGGCGAAAACGAATAAGCAATTCCGCAGAGTGCAGATTGTAACAGCTGCCCTGCAATCGTA
TACACACGGAACAAACGATGCGCAAAAAGCAATGGGAATTATCACAATGGCTCTCATCACAGCGAATTTACATACTTCTG
CTAATGATATTCCTACCTGGGTGCAATTCGCCTGCGCGACAGCCATGGGTCTCGGAACATCAATCGGCGGCTGGAAAATC
ATCAAAACCGTTGGCGGAAAGATCATGAAAATCCGTCCGGTAAACGGTGTTTCCGCTGACTTAACCGGAGCAGCCATTAT
TTTCGGCGCCACGTTTATTCATTTGCCGGTCAGTACAACCCACGTCATTTCTTCATCAATTCTTGGCGTCGGTGCGTCCC
ACCGCGTAAAAGGCGTAAACTGGGGTACGGCCAAGCGAATGCTCATTACATGGGTCATCACGCTTCCGATTTCAGCGACA
CTTGGTGCCATCGCCTACTTTATTTTAAATATGATATTTTAA

Upstream 100 bases:

>100_bases
TGAAATATACGAAACGCTTGAAGAAATCGCCGACTCTTGTCAAAGTGTTGCCAATAATCTTGAAACCATCATTATGAAAA
ACGCGTAACGAGGGGTAAAA

Downstream 100 bases:

>100_bases
TGTCACACCGCTTCTGTCAAAGGAGCGGTTTTTTTGATGGAACAGGAAAACCATCCGGATCTCTCGAATACAATAAGCAG
AACCTATTTCAGATGTGAGG

Product: low-affinity inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MDILLILTILIVICALAFDFINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAMTFTGVAKTITKDIVDPYTLENG
SVVILAALLAAIAWNLITWYYGIPSSSSHAIIGAIAGAAIAAAGFAALNYKGFIKIIEALILSPIIAFVLGFILYSIVKL
IFKDSNLAKTNKQFRRVQIVTAALQSYTHGTNDAQKAMGIITMALITANLHTSANDIPTWVQFACATAMGLGTSIGGWKI
IKTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPISAT
LGAIAYFILNMIF

Sequences:

>Translated_333_residues
MDILLILTILIVICALAFDFINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAMTFTGVAKTITKDIVDPYTLENG
SVVILAALLAAIAWNLITWYYGIPSSSSHAIIGAIAGAAIAAAGFAALNYKGFIKIIEALILSPIIAFVLGFILYSIVKL
IFKDSNLAKTNKQFRRVQIVTAALQSYTHGTNDAQKAMGIITMALITANLHTSANDIPTWVQFACATAMGLGTSIGGWKI
IKTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPISAT
LGAIAYFILNMIF
>Mature_333_residues
MDILLILTILIVICALAFDFINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAMTFTGVAKTITKDIVDPYTLENG
SVVILAALLAAIAWNLITWYYGIPSSSSHAIIGAIAGAAIAAAGFAALNYKGFIKIIEALILSPIIAFVLGFILYSIVKL
IFKDSNLAKTNKQFRRVQIVTAALQSYTHGTNDAQKAMGIITMALITANLHTSANDIPTWVQFACATAMGLGTSIGGWKI
IKTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPISAT
LGAIAYFILNMIF

Specific function: Low-affinity inorganic phosphate transport

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily

Homologues:

Organism=Homo sapiens, GI31543630, Length=175, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=30.2631578947368, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789360, Length=217, Percent_Identity=32.7188940092166, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=32.258064516129, Blast_Score=112, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI25146401, Length=163, Percent_Identity=31.2883435582822, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17539280, Length=165, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71981576, Length=163, Percent_Identity=30.6748466257669, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI32566716, Length=178, Percent_Identity=24.7191011235955, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=151, Percent_Identity=31.1258278145695, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIT_BACSU (O34436)

Other databases:

- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   A69855
- RefSeq:   NP_389167.2
- EnsemblBacteria:   EBBACT00000001755
- GeneID:   936281
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12840
- NMPDR:   fig|224308.1.peg.1286
- GenoList:   BSU12840
- GeneTree:   EBGT00050000001970
- HOGENOM:   HBG681698
- ProtClustDB:   CLSK884804
- BioCyc:   BSUB:BSU12840-MONOMER
- InterPro:   IPR001204

Pfam domain/function: PF01384 PHO4

EC number: NA

Molecular weight: Translated: 35231; Mature: 35231

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe34e5cc)-; HASH(0xe682214)-; HASH(0xc95a81c)-; HASH(0xe549f80)-; HASH(0xcb3ee58)-; HASH(0xc95a0c0)-; HASH(0xe0e8d30)-; HASH(0xb544f68)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILLILTILIVICALAFDFINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAMTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVDPYTLENGSVVILAALLAAIAWNLITWYYGIPSSSSHAIIGAIAGAAI
HHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AAAGFAALNYKGFIKIIEALILSPIIAFVLGFILYSIVKLIFKDSNLAKTNKQFRRVQIV
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TAALQSYTHGTNDAQKAMGIITMALITANLHTSANDIPTWVQFACATAMGLGTSIGGWKI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
IKTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVN
HHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCC
WGTAKRMLITWVITLPISATLGAIAYFILNMIF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
>Mature Secondary Structure
MDILLILTILIVICALAFDFINGFHDTANAIATSVSTKALKPRHAIILAAVMNFVGAMTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITKDIVDPYTLENGSVVILAALLAAIAWNLITWYYGIPSSSSHAIIGAIAGAAI
HHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
AAAGFAALNYKGFIKIIEALILSPIIAFVLGFILYSIVKLIFKDSNLAKTNKQFRRVQIV
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TAALQSYTHGTNDAQKAMGIITMALITANLHTSANDIPTWVQFACATAMGLGTSIGGWKI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
IKTVGGKIMKIRPVNGVSADLTGAAIIFGATFIHLPVSTTHVISSSILGVGASHRVKGVN
HHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHCCCCCCCCCCCC
WGTAKRMLITWVITLPISATLGAIAYFILNMIF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377