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The map label for this gene is spoIISA

Identifier: 16078348

GI number: 16078348

Start: 1348612

End: 1349358

Strand: Reverse

Name: spoIISA

Synonym: BSU12830

Alternate gene names: 16078348

Gene position: 1349358-1348612 (Counterclockwise)

Preceding gene: 255767297

Following gene: 16078347

Centisome position: 32.01

GC content: 42.17

Gene sequence:

>747_bases
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Upstream 100 bases:

>100_bases
GCTTCTGTCAAAGGAGCGGTTTTTTTGATGGAACAGGAAAACCATCCGGATCTCTCGAATACAATAAGCAGAACCTATTT
CAGATGTGAGGGATTCGAAT

Downstream 100 bases:

>100_bases
TGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAGT
GTCGCAAGCTATCAAGTCAG

Product: two-component apoptosis factor

Products: NA

Alternate protein names: Killer protein spoIISA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Sequences:

>Translated_248_residues
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG
>Mature_248_residues
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Specific function: Has a negative effect on sporulation. It is lethal when synthesized during vegetative growth in the absence of SpoIISB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SP2SA_BACSU (O34853)

Other databases:

- EMBL:   AJ002571
- EMBL:   AL009126
- PIR:   D69713
- RefSeq:   NP_389166.1
- ProteinModelPortal:   O34853
- EnsemblBacteria:   EBBACT00000000823
- GeneID:   938174
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU12830
- NMPDR:   fig|224308.1.peg.1285
- GenoList:   BSU12830
- GeneTree:   EBGT00050000001707
- HOGENOM:   HBG337938
- OMA:   YLLKNEP
- ProtClustDB:   CLSK887145
- BioCyc:   BSUB:BSU12830-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29062; Mature: 29062

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe4a24ac)-; HASH(0xe736f30)-; HASH(0xe608624)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSL
CCHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FTHWERYLIVAVSFALIDAFIFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
QYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASLCHYSTQADKDRLTEHMDDPA
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHCCCCH
DVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
HHHHHCCHHHCCHHCCCCEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
PIEEEGEG
CCCCCCCH
>Mature Secondary Structure
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSL
CCHHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
FTHWERYLIVAVSFALIDAFIFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
QYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASLCHYSTQADKDRLTEHMDDPA
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHCCCCH
DVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
HHHHHCCHHHCCHHCCCCEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
PIEEEGEG
CCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 11371520