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The map label for this gene is phoA

Identifier: 255767221

GI number: 255767221

Start: 1017083

End: 1018468

Strand: Reverse

Name: phoA

Synonym: BSU09410

Alternate gene names: 255767221

Gene position: 1018468-1017083 (Counterclockwise)

Preceding gene: 255767223

Following gene: 16078004

Centisome position: 24.16

GC content: 44.95

Gene sequence:

>1386_bases
ATGAAAAAAATGAGTTTGTTTCAAAATATGAAATCAAAACTTCTGCCAATCGCCGCTGTTTCTGTCCTTACAGCTGGAAT
CTTTGCCGGAGCTGAGCTTCAGCAAACAGAAAAGGCCAGCGCCAAAAAACAAGACAAAGCTGAGATCAGAAATGTCATTG
TGATGATAGGCGACGGCATGGGGACGCCTTACATAAGAGCCTACCGTTCCATGAAAAATAACGGTGACACACCGAATAAC
CCGAAGTTAACAGAATTTGACCGGAACCTGACAGGCATGATGATGACGCATCCGGATGACCCTGACTATAATATTACAGA
TTCAGCAGCAGCCGGAACAGCATTAGCGACAGGCGTTAAGACATATAACAATGCAATTGGCGTCGATAAAAACGGAAAAA
AAGTGAAATCTGTACTTGAAGAGGCCAAACAGCAAGGCAAGTCAACAGGGCTTGTCGCCACGTCTGAAATTAACCACGCC
ACTCCAGCCGCATATGGCGCCCACAATGAATCACGGAAAAACATGGACCAAATCGCCAACAGCTATATGGATGACAAGAT
AAAAGGCAAACATAAAATAGACGTGCTGCTCGGCGGCGGAAAATCTTATTTTAACCGCAAGGACAGAAACTTGACAAAGG
AATTCAAACAAGCCGGCTACAGCTATGTGACAACTAAACAAGCATTGAAAAAAAATAAAGATCAGCAGGTGCTCGGGCTT
TTCGCAGATGGAGGGCTTGCTAAAGCGCTCGACCGTGACAGTAAAACACCGTCTCTCAAAGACATGACGGTTTCAGCAAT
TGATCGCCTGAACCAAAATAAAAAAGGATTTTTCTTGATGGTCGAAGGGAGCCAGATTGACTGGGCGGCCCATGACAATG
ATACAGTAGGAGCCATGAGCGAGGTTAAAGATTTTGAACAGGCCTATAAAGCCGCGATTGAATTTGCGAAAAAAGACAAA
CATACACTTGTGATTGCAACTGCTGACCATACAACCGGCGGCTTTACCATTGGCGCAAACGGGGAAAAGAATTGGCACGC
AGAACCGATTCTCTCCGCTAAGAAAACACCTGAATTCATGGCCAAAAAAATCAGTGAAGGCAAGCCGGTTAAAGATGTGC
TCGCCCGCTATGCCAATCTGAAAGTCACATCTGAAGAAATCAAAAGCGTTGAAGCAGCTGCACAGGCTGACAAAAGCAAA
GGGGCCTCCAAAGCCATCATCAAGATTTTTAATACCCGCTCCAACAGCGGATGGACGAGTACCGATCATACCGGCGAAGA
AGTACCGGTATACGCGTACGGCCCCGGAAAAGAAAAATTCCGCGGATTGATTAACAATACGGACCAGGCAAACATCATAT
TTAAGATTTTAAAAACTGGAAAATAA

Upstream 100 bases:

>100_bases
TTTTCATTTCCTTACAAGGCTTTACTTATTGTTTACATGATCAACAGCCGCATTTAACAAAGTTTCCCTAACATGATAAA
CGGAATACATTAAAGGAGGC

Downstream 100 bases:

>100_bases
AAAGGATGCAGAACGCTGCATCCTCTTTTTTATAAATATCTGCGGTGAACACCTTTTCCGTCGTACGAAAACATGACTTC
CTTATCCTCAAGCACAGACT

Product: alkaline phosphatase A

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKKMSLFQNMKSKLLPIAAVSVLTAGIFAGAELQQTEKASAKKQDKAEIRNVIVMIGDGMGTPYIRAYRSMKNNGDTPNN
PKLTEFDRNLTGMMMTHPDDPDYNITDSAAAGTALATGVKTYNNAIGVDKNGKKVKSVLEEAKQQGKSTGLVATSEINHA
TPAAYGAHNESRKNMDQIANSYMDDKIKGKHKIDVLLGGGKSYFNRKDRNLTKEFKQAGYSYVTTKQALKKNKDQQVLGL
FADGGLAKALDRDSKTPSLKDMTVSAIDRLNQNKKGFFLMVEGSQIDWAAHDNDTVGAMSEVKDFEQAYKAAIEFAKKDK
HTLVIATADHTTGGFTIGANGEKNWHAEPILSAKKTPEFMAKKISEGKPVKDVLARYANLKVTSEEIKSVEAAAQADKSK
GASKAIIKIFNTRSNSGWTSTDHTGEEVPVYAYGPGKEKFRGLINNTDQANIIFKILKTGK

Sequences:

>Translated_461_residues
MKKMSLFQNMKSKLLPIAAVSVLTAGIFAGAELQQTEKASAKKQDKAEIRNVIVMIGDGMGTPYIRAYRSMKNNGDTPNN
PKLTEFDRNLTGMMMTHPDDPDYNITDSAAAGTALATGVKTYNNAIGVDKNGKKVKSVLEEAKQQGKSTGLVATSEINHA
TPAAYGAHNESRKNMDQIANSYMDDKIKGKHKIDVLLGGGKSYFNRKDRNLTKEFKQAGYSYVTTKQALKKNKDQQVLGL
FADGGLAKALDRDSKTPSLKDMTVSAIDRLNQNKKGFFLMVEGSQIDWAAHDNDTVGAMSEVKDFEQAYKAAIEFAKKDK
HTLVIATADHTTGGFTIGANGEKNWHAEPILSAKKTPEFMAKKISEGKPVKDVLARYANLKVTSEEIKSVEAAAQADKSK
GASKAIIKIFNTRSNSGWTSTDHTGEEVPVYAYGPGKEKFRGLINNTDQANIIFKILKTGK
>Mature_461_residues
MKKMSLFQNMKSKLLPIAAVSVLTAGIFAGAELQQTEKASAKKQDKAEIRNVIVMIGDGMGTPYIRAYRSMKNNGDTPNN
PKLTEFDRNLTGMMMTHPDDPDYNITDSAAAGTALATGVKTYNNAIGVDKNGKKVKSVLEEAKQQGKSTGLVATSEINHA
TPAAYGAHNESRKNMDQIANSYMDDKIKGKHKIDVLLGGGKSYFNRKDRNLTKEFKQAGYSYVTTKQALKKNKDQQVLGL
FADGGLAKALDRDSKTPSLKDMTVSAIDRLNQNKKGFFLMVEGSQIDWAAHDNDTVGAMSEVKDFEQAYKAAIEFAKKDK
HTLVIATADHTTGGFTIGANGEKNWHAEPILSAKKTPEFMAKKISEGKPVKDVLARYANLKVTSEEIKSVEAAAQADKSK
GASKAIIKIFNTRSNSGWTSTDHTGEEVPVYAYGPGKEKFRGLINNTDQANIIFKILKTGK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family

Homologues:

Organism=Homo sapiens, GI116734717, Length=452, Percent_Identity=27.8761061946903, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI157266292, Length=337, Percent_Identity=30.2670623145401, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI94721246, Length=349, Percent_Identity=29.512893982808, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI157266296, Length=350, Percent_Identity=30.5714285714286, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI294660770, Length=445, Percent_Identity=27.4157303370787, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI294660772, Length=391, Percent_Identity=27.6214833759591, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI48994877, Length=460, Percent_Identity=31.9565217391304, Blast_Score=165, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6320689, Length=445, Percent_Identity=31.9101123595506, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI18859923, Length=468, Percent_Identity=26.4957264957265, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24651554, Length=493, Percent_Identity=29.4117647058824, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21355981, Length=458, Percent_Identity=29.9126637554585, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21355151, Length=473, Percent_Identity=27.906976744186, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24651556, Length=417, Percent_Identity=29.7362110311751, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI19921912, Length=368, Percent_Identity=32.3369565217391, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355149, Length=468, Percent_Identity=27.5641025641026, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI85725200, Length=448, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI85815833, Length=448, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24585213, Length=492, Percent_Identity=25.4065040650407, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358067, Length=450, Percent_Identity=28, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24657842, Length=472, Percent_Identity=26.271186440678, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24657835, Length=353, Percent_Identity=27.1954674220963, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24657827, Length=338, Percent_Identity=28.698224852071, Blast_Score=93, Evalue=5e-19,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PPB4_BACSU (P19406)

Other databases:

- EMBL:   U02550
- EMBL:   Y14082
- EMBL:   AL009126
- EMBL:   L26337
- PIR:   B69676
- RefSeq:   NP_388822.2
- ProteinModelPortal:   P19406
- SMR:   P19406
- EnsemblBacteria:   EBBACT00000003817
- GeneID:   936265
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09410
- NMPDR:   fig|224308.1.peg.941
- GenoList:   BSU09410
- GeneTree:   EBGT00050000002942
- HOGENOM:   HBG617166
- ProtClustDB:   CLSK2460902
- BioCyc:   BSUB:BSU09410-MONOMER
- BRENDA:   3.1.3.1
- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850
- Gene3D:   G3DSA:3.40.720.10
- PRINTS:   PR00113
- SMART:   SM00098

Pfam domain/function: PF00245 Alk_phosphatase; SSF53649 Alkaline_phosphatase_core

EC number: =3.1.3.1

Molecular weight: Translated: 50275; Mature: 50275

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: ACT_SITE 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMSLFQNMKSKLLPIAAVSVLTAGIFAGAELQQTEKASAKKQDKAEIRNVIVMIGDGM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCC
GTPYIRAYRSMKNNGDTPNNPKLTEFDRNLTGMMMTHPDDPDYNITDSAAAGTALATGVK
CCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCHHCCHHHHHHHH
TYNNAIGVDKNGKKVKSVLEEAKQQGKSTGLVATSEINHATPAAYGAHNESRKNMDQIAN
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
SYMDDKIKGKHKIDVLLGGGKSYFNRKDRNLTKEFKQAGYSYVTTKQALKKNKDQQVLGL
HHHCCCCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEE
FADGGLAKALDRDSKTPSLKDMTVSAIDRLNQNKKGFFLMVEGSQIDWAAHDNDTVGAMS
ECCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCCHHHHH
EVKDFEQAYKAAIEFAKKDKHTLVIATADHTTGGFTIGANGEKNWHAEPILSAKKTPEFM
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHH
AKKISEGKPVKDVLARYANLKVTSEEIKSVEAAAQADKSKGASKAIIKIFNTRSNSGWTS
HHHHCCCCCHHHHHHHHHCCEECHHHHHHHHHHHHHHHHCCCHHEEHEEEECCCCCCCCC
TDHTGEEVPVYAYGPGKEKFRGLINNTDQANIIFKILKTGK
CCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKMSLFQNMKSKLLPIAAVSVLTAGIFAGAELQQTEKASAKKQDKAEIRNVIVMIGDGM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCEEEEEEECCC
GTPYIRAYRSMKNNGDTPNNPKLTEFDRNLTGMMMTHPDDPDYNITDSAAAGTALATGVK
CCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEECCCCCCCCCCCCHHCCHHHHHHHH
TYNNAIGVDKNGKKVKSVLEEAKQQGKSTGLVATSEINHATPAAYGAHNESRKNMDQIAN
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
SYMDDKIKGKHKIDVLLGGGKSYFNRKDRNLTKEFKQAGYSYVTTKQALKKNKDQQVLGL
HHHCCCCCCCEEEEEEECCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEE
FADGGLAKALDRDSKTPSLKDMTVSAIDRLNQNKKGFFLMVEGSQIDWAAHDNDTVGAMS
ECCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCEEEEECCCCCCHHHHH
EVKDFEQAYKAAIEFAKKDKHTLVIATADHTTGGFTIGANGEKNWHAEPILSAKKTPEFM
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHH
AKKISEGKPVKDVLARYANLKVTSEEIKSVEAAAQADKSKGASKAIIKIFNTRSNSGWTS
HHHHCCCCCHHHHHHHHHCCEECHHHHHHHHHHHHHHHHCCCHHEEHEEEECCCCCCCCC
TDHTGEEVPVYAYGPGKEKFRGLINNTDQANIIFKILKTGK
CCCCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469