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The map label for this gene is citR

Identifier: 255767223

GI number: 255767223

Start: 1020073

End: 1020948

Strand: Reverse

Name: citR

Synonym: BSU09430

Alternate gene names: 255767223

Gene position: 1020948-1020073 (Counterclockwise)

Preceding gene: 16078013

Following gene: 255767221

Centisome position: 24.22

GC content: 43.72

Gene sequence:

>876_bases
ATGGATTTCAAATGGCTTCACACCTTTGTGACCGCTGCAAAATATGAGAACTTCCGAAAAACGGCGGAAACGCTTTTCTT
ATCTCAGCCTACTGTGACCGTACATATCAAGCAATTAGAAAAAGAAATCAGCTGCAAGCTGTTCGAGCGTAAAGGCAGGC
AAATCCAGCTGACTGATGAAGGCAGGGCGTATTTGCCGTATGCGCTCAGGCTTCTTGATGATTATGAAAACAGCATGGCA
GAGCTTCACAGAGTGAGACAAGGCTACTCTCAAACCTTACAGCTCGCGGTTTCTCCGCTTATTGCAGATACAGTGCTTCC
TTCTGTCATGAAGCGGTACACAGCAATGAATACGGAAACAGAAATGGCGGTTACGATTTTCGAATCAGCGGAAATCGCGT
CACTGATTAAAGCCGGGGAAGCAGATATCGGACTAAGCTGTTTAAAGGTACAGTCTTCTTCTTTAAGCTGCCACTGCTTA
TACAAAGACCCTGTCGTCCTTGTTGCCCCGCCCGATAAGCGTTTCATAGAAGACAACGAAATTGATGCCAAAGAGGTGCT
CGAACAATACCTGCTTTTGACCCACAATCATCCTGATTACTGGGACGACTTGCTCCGTCAAGTCAGAATGACATTTCCAT
TTGTGAGAACAATGAAGGTCACTCAAACACATATAACGAAACGGTTTATCAAGGAAGGACTCGGAGTTTCATTTCTGCCT
TTATCTACAGTAAAGCGTGAGTTAGCAGAGAAACAGATGATCAGAATTCCTTATCAGTCTGTACAGCTGCCCTATGCCGG
CGCTTACGCGATTGCCCTTTATGAAAATAAGAAAGAAAAAAAGTTCTTGGATTTTTTATCACATTTTCATTTTTAG

Upstream 100 bases:

>100_bases
CCCCTATCCTTTTCTGTTATTATAGTAAATATAATATTTAAAAAATAATCAATATTTTAAAGGGAATTAATTAAAAATAT
TAATCAGAGGGAGAATAGAA

Downstream 100 bases:

>100_bases
GAGCAACCAAAACGCCTGTGCTCCGTCTAATGAAAAAAACCCGTTCCAATGAACGGGTTTTCTCTAAAAATTAGAATCTT
TTCGCACCGAGGTAACGAGG

Product: transcriptional regulator CitR

Products: NA

Alternate protein names: Citrate synthase I repressor

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MDFKWLHTFVTAAKYENFRKTAETLFLSQPTVTVHIKQLEKEISCKLFERKGRQIQLTDEGRAYLPYALRLLDDYENSMA
ELHRVRQGYSQTLQLAVSPLIADTVLPSVMKRYTAMNTETEMAVTIFESAEIASLIKAGEADIGLSCLKVQSSSLSCHCL
YKDPVVLVAPPDKRFIEDNEIDAKEVLEQYLLLTHNHPDYWDDLLRQVRMTFPFVRTMKVTQTHITKRFIKEGLGVSFLP
LSTVKRELAEKQMIRIPYQSVQLPYAGAYAIALYENKKEKKFLDFLSHFHF

Sequences:

>Translated_291_residues
MDFKWLHTFVTAAKYENFRKTAETLFLSQPTVTVHIKQLEKEISCKLFERKGRQIQLTDEGRAYLPYALRLLDDYENSMA
ELHRVRQGYSQTLQLAVSPLIADTVLPSVMKRYTAMNTETEMAVTIFESAEIASLIKAGEADIGLSCLKVQSSSLSCHCL
YKDPVVLVAPPDKRFIEDNEIDAKEVLEQYLLLTHNHPDYWDDLLRQVRMTFPFVRTMKVTQTHITKRFIKEGLGVSFLP
LSTVKRELAEKQMIRIPYQSVQLPYAGAYAIALYENKKEKKFLDFLSHFHF
>Mature_291_residues
MDFKWLHTFVTAAKYENFRKTAETLFLSQPTVTVHIKQLEKEISCKLFERKGRQIQLTDEGRAYLPYALRLLDDYENSMA
ELHRVRQGYSQTLQLAVSPLIADTVLPSVMKRYTAMNTETEMAVTIFESAEIASLIKAGEADIGLSCLKVQSSSLSCHCL
YKDPVVLVAPPDKRFIEDNEIDAKEVLEQYLLLTHNHPDYWDDLLRQVRMTFPFVRTMKVTQTHITKRFIKEGLGVSFLP
LSTVKRELAEKQMIRIPYQSVQLPYAGAYAIALYENKKEKKFLDFLSHFHF

Specific function: Negative regulatory protein for the citA gene for citrate synthase I

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI87081904, Length=259, Percent_Identity=26.6409266409266, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI87082132, Length=266, Percent_Identity=26.3157894736842, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=26.8292682926829, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788296, Length=200, Percent_Identity=30.5, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787879, Length=268, Percent_Identity=26.1194029850746, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI157672245, Length=168, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367136, Length=241, Percent_Identity=24.0663900414938, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790783, Length=303, Percent_Identity=24.0924092409241, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI145693105, Length=237, Percent_Identity=21.9409282700422, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786401, Length=302, Percent_Identity=21.8543046357616, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790399, Length=271, Percent_Identity=21.0332103321033, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788706, Length=169, Percent_Identity=30.7692307692308, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): CITR_BACSU (P39127)

Other databases:

- EMBL:   U05256
- EMBL:   Y14082
- EMBL:   AL009126
- PIR:   I40379
- RefSeq:   NP_388824.2
- ProteinModelPortal:   P39127
- SMR:   P39127
- EnsemblBacteria:   EBBACT00000003211
- GeneID:   939267
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09430
- NMPDR:   fig|224308.1.peg.943
- GenoList:   BSU09430
- GeneTree:   EBGT00050000000807
- HOGENOM:   HBG338672
- ProtClustDB:   CLSK886957
- BioCyc:   BSUB:BSU09430-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33667; Mature: 33667

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKWLHTFVTAAKYENFRKTAETLFLSQPTVTVHIKQLEKEISCKLFERKGRQIQLTDE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECC
GRAYLPYALRLLDDYENSMAELHRVRQGYSQTLQLAVSPLIADTVLPSVMKRYTAMNTET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EMAVTIFESAEIASLIKAGEADIGLSCLKVQSSSLSCHCLYKDPVVLVAPPDKRFIEDNE
CEEEEEECHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCCCCCCCC
IDAKEVLEQYLLLTHNHPDYWDDLLRQVRMTFPFVRTMKVTQTHITKRFIKEGLGVSFLP
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
LSTVKRELAEKQMIRIPYQSVQLPYAGAYAIALYENKKEKKFLDFLSHFHF
HHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFKWLHTFVTAAKYENFRKTAETLFLSQPTVTVHIKQLEKEISCKLFERKGRQIQLTDE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCEEEEECC
GRAYLPYALRLLDDYENSMAELHRVRQGYSQTLQLAVSPLIADTVLPSVMKRYTAMNTET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EMAVTIFESAEIASLIKAGEADIGLSCLKVQSSSLSCHCLYKDPVVLVAPPDKRFIEDNE
CEEEEEECHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCCCCCCCC
IDAKEVLEQYLLLTHNHPDYWDDLLRQVRMTFPFVRTMKVTQTHITKRFIKEGLGVSFLP
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
LSTVKRELAEKQMIRIPYQSVQLPYAGAYAIALYENKKEKKFLDFLSHFHF
HHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377; 8045899