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The map label for this gene is glpD

Identifier: 255767218

GI number: 255767218

Start: 1004975

End: 1006642

Strand: Direct

Name: glpD

Synonym: BSU09300

Alternate gene names: 255767218

Gene position: 1004975-1006642 (Clockwise)

Preceding gene: 255767217

Following gene: 255767219

Centisome position: 23.84

GC content: 45.44

Gene sequence:

>1668_bases
ATGATGAATCATCAATTTTCAAGTCTTGAAAGAGATCGCATGCTGACAGACATGACGAAAAAAACATATGACCTATTTAT
TATCGGAGGAGGAATTACAGGAGCCGGAACAGCTCTTGACGCGGCATCAAGGGGAATGAAGGTCGCACTAAGCGAAATGC
AGGACTTTGCGGCGGGAACATCAAGCCGATCAACGAAGCTGGTACATGGCGGCTTGCGCTATTTAAAACAATTTGAAGTG
AAAATGGTCGCTGAGGTAGGAAAAGAGCGGGCGATTGTGTATGAAAACGGCCCGCATGTCACTACACCGGAATGGATGCT
GCTTCCGTTTCATAAAGGCGGCACATTCGGTTCATTTACAACATCTATTGGGTTAAGGGTTTATGACTTCCTTGCAGGTG
TGAAAAAGTCAGAACGAAGAAGCATGCTTTCAGCAAAAGAAACGCTGCAAAAAGAGCCTTTGGTGAAAAAAGACGGCTTA
AAAGGCGGCGGGTACTATGTGGAATACCGCACTGACGATGCGAGACTGACCATCGAAGTCATGAAGGAAGCGGTTAAATT
CGGGGCAGAGCCTGTGAATTACTCCAAAGTGAAGGAGCTTCTTTACGAAAAAGGCAAAGCCGTCGGCGTATTAATTGAAG
ATGTGCTGACAAAGAAAGAATATAAAGTGTATGCGAAAAAAATTGTCAATGCTACAGGCCCTTGGGTCGATCAGCTCAGA
GAAAAAGACCATTCGAAAAACGGAAAGCATTTGCAGCATACAAAAGGCATTCACCTTGTATTTGACCAGTCTGTCTTTCC
GCTGAAACAGGCTGTATATTTCGATACACCTGATGGCCGGATGGTATTTGCGATTCCTCGTGAAGGCAAAACATACGTGG
GAACAACAGACACTGTTTACAAAGAGGCGCTGGAGCATCCGCGGATGACAACGGAGGATCGTGATTATGTCATCAAATCA
ATCAATTACATGTTCCCGGAACTGAATATCACTGCGAATGACATCGAATCCAGCTGGGCGGGGCTGCGTCCGCTGATTCA
TGAAGAAGGCAAAGATCCTTCTGAAATTTCACGGAAAGACGAGATTTGGACATCTGACTCAGGCCTGATCACCATTGCCG
GCGGAAAACTGACTGGATACAGAAAAATGGCGGAGCACATCGTTGATCTTGTCCGTGATCGCTTAAAAGAAGAGGGCGAA
AAGGATTTTGGACCATGTAAAACGAAAAACATGCCAATCTCTGGCGGGCACGTCGGCGGTTCGAAAAATCTTATGTCCTT
CGTTACCGCGAAAACAAAAGAAGGAATTGCAGCCGGTTTATCAGAAAAAGACGCAAAACAGCTTGCGATCAGATACGGCT
CTAACGTAGATCGCGTCTTTGACCGGGTAGAAGCGCTGAAAGATGAGGCCGCGAAACGCAACATTCCGGTTCATATTCTT
GCTGAGGCAGAATACAGTATAGAAGAAGAGATGACTGCAACCCCTGCTGACTTCTTTGTCCGCAGAACGGGACGTTTATT
TTTTGATATCAATTGGGTAAGAACATATAAAGATGCCGTTATTGATTTTATGAGCGAGCGATTCCAATGGGATGAGCAGG
CGAAAAACAAACATACAGAAAACCTCAACAAGCTTTTACACGATGCGGTCGTACCGCTTGAGCAATAA

Upstream 100 bases:

>100_bases
GTCCTGAGATGAGGAGAGACCACAGCACCAAAGTGTAAGCATGCACTTTGGCTGTTGTGGTCTCTTTTTCTATTTACCGT
GACAACAAGGAGGAAACGTA

Downstream 100 bases:

>100_bases
ATCATAACGGGCTGTCTGCAGCCCGTTATTTCTTTTTACGTGCCGAAAGGGGGAGATCTCAGGTTATCAAGTGACAGAGG
CACCCGCTTCATATAAAATA

Product: glycerol-3-phosphate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MMNHQFSSLERDRMLTDMTKKTYDLFIIGGGITGAGTALDAASRGMKVALSEMQDFAAGTSSRSTKLVHGGLRYLKQFEV
KMVAEVGKERAIVYENGPHVTTPEWMLLPFHKGGTFGSFTTSIGLRVYDFLAGVKKSERRSMLSAKETLQKEPLVKKDGL
KGGGYYVEYRTDDARLTIEVMKEAVKFGAEPVNYSKVKELLYEKGKAVGVLIEDVLTKKEYKVYAKKIVNATGPWVDQLR
EKDHSKNGKHLQHTKGIHLVFDQSVFPLKQAVYFDTPDGRMVFAIPREGKTYVGTTDTVYKEALEHPRMTTEDRDYVIKS
INYMFPELNITANDIESSWAGLRPLIHEEGKDPSEISRKDEIWTSDSGLITIAGGKLTGYRKMAEHIVDLVRDRLKEEGE
KDFGPCKTKNMPISGGHVGGSKNLMSFVTAKTKEGIAAGLSEKDAKQLAIRYGSNVDRVFDRVEALKDEAAKRNIPVHIL
AEAEYSIEEEMTATPADFFVRRTGRLFFDINWVRTYKDAVIDFMSERFQWDEQAKNKHTENLNKLLHDAVVPLEQ

Sequences:

>Translated_555_residues
MMNHQFSSLERDRMLTDMTKKTYDLFIIGGGITGAGTALDAASRGMKVALSEMQDFAAGTSSRSTKLVHGGLRYLKQFEV
KMVAEVGKERAIVYENGPHVTTPEWMLLPFHKGGTFGSFTTSIGLRVYDFLAGVKKSERRSMLSAKETLQKEPLVKKDGL
KGGGYYVEYRTDDARLTIEVMKEAVKFGAEPVNYSKVKELLYEKGKAVGVLIEDVLTKKEYKVYAKKIVNATGPWVDQLR
EKDHSKNGKHLQHTKGIHLVFDQSVFPLKQAVYFDTPDGRMVFAIPREGKTYVGTTDTVYKEALEHPRMTTEDRDYVIKS
INYMFPELNITANDIESSWAGLRPLIHEEGKDPSEISRKDEIWTSDSGLITIAGGKLTGYRKMAEHIVDLVRDRLKEEGE
KDFGPCKTKNMPISGGHVGGSKNLMSFVTAKTKEGIAAGLSEKDAKQLAIRYGSNVDRVFDRVEALKDEAAKRNIPVHIL
AEAEYSIEEEMTATPADFFVRRTGRLFFDINWVRTYKDAVIDFMSERFQWDEQAKNKHTENLNKLLHDAVVPLEQ
>Mature_555_residues
MMNHQFSSLERDRMLTDMTKKTYDLFIIGGGITGAGTALDAASRGMKVALSEMQDFAAGTSSRSTKLVHGGLRYLKQFEV
KMVAEVGKERAIVYENGPHVTTPEWMLLPFHKGGTFGSFTTSIGLRVYDFLAGVKKSERRSMLSAKETLQKEPLVKKDGL
KGGGYYVEYRTDDARLTIEVMKEAVKFGAEPVNYSKVKELLYEKGKAVGVLIEDVLTKKEYKVYAKKIVNATGPWVDQLR
EKDHSKNGKHLQHTKGIHLVFDQSVFPLKQAVYFDTPDGRMVFAIPREGKTYVGTTDTVYKEALEHPRMTTEDRDYVIKS
INYMFPELNITANDIESSWAGLRPLIHEEGKDPSEISRKDEIWTSDSGLITIAGGKLTGYRKMAEHIVDLVRDRLKEEGE
KDFGPCKTKNMPISGGHVGGSKNLMSFVTAKTKEGIAAGLSEKDAKQLAIRYGSNVDRVFDRVEALKDEAAKRNIPVHIL
AEAEYSIEEEMTATPADFFVRRTGRLFFDINWVRTYKDAVIDFMSERFQWDEQAKNKHTENLNKLLHDAVVPLEQ

Specific function: Conversion Of Glycerol 3-Phosphate To Dihydroxyacetone. Uses Molecular Oxygen Or Nitrate As Electron Acceptor. [C]

COG id: COG0578

COG function: function code C; Glycerol-3-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI285002233, Length=561, Percent_Identity=32.7985739750446, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI285002231, Length=561, Percent_Identity=32.7985739750446, Blast_Score=281, Evalue=1e-75,
Organism=Escherichia coli, GI2367226, Length=467, Percent_Identity=30.6209850107066, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI1788574, Length=392, Percent_Identity=26.0204081632653, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17509253, Length=553, Percent_Identity=31.4647377938517, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI115534726, Length=533, Percent_Identity=32.8330206378987, Blast_Score=238, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6322036, Length=596, Percent_Identity=31.2080536912752, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI161077125, Length=560, Percent_Identity=33.2142857142857, Blast_Score=257, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24653942, Length=560, Percent_Identity=33.2142857142857, Blast_Score=257, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24653944, Length=560, Percent_Identity=33.2142857142857, Blast_Score=257, Evalue=2e-68,
Organism=Drosophila melanogaster, GI20130025, Length=560, Percent_Identity=33.2142857142857, Blast_Score=257, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24586295, Length=566, Percent_Identity=27.5618374558304, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI19921278, Length=528, Percent_Identity=26.1363636363636, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI45550977, Length=407, Percent_Identity=24.3243243243243, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPD_BACSU (P18158)

Other databases:

- EMBL:   M34393
- EMBL:   Y14079
- EMBL:   AL009126
- PIR:   C45868
- RefSeq:   NP_388811.2
- ProteinModelPortal:   P18158
- SMR:   P18158
- EnsemblBacteria:   EBBACT00000002306
- GeneID:   936250
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU09300
- NMPDR:   fig|224308.1.peg.930
- GenoList:   BSU09300
- GeneTree:   EBGT00050000001494
- HOGENOM:   HBG720894
- ProtClustDB:   CLSK2523426
- BioCyc:   BSUB:BSU09300-MONOMER
- BRENDA:   1.1.99.5
- InterPro:   IPR006076
- InterPro:   IPR000447
- PRINTS:   PR01001

Pfam domain/function: PF01266 DAO

EC number: =1.1.5.3

Molecular weight: Translated: 62568; Mature: 62568

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00977 FAD_G3PDH_1; PS00978 FAD_G3PDH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNHQFSSLERDRMLTDMTKKTYDLFIIGGGITGAGTALDAASRGMKVALSEMQDFAAGT
CCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHCCC
SSRSTKLVHGGLRYLKQFEVKMVAEVGKERAIVYENGPHVTTPEWMLLPFHKGGTFGSFT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCHH
TSIGLRVYDFLAGVKKSERRSMLSAKETLQKEPLVKKDGLKGGGYYVEYRTDDARLTIEV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEHHHH
MKEAVKFGAEPVNYSKVKELLYEKGKAVGVLIEDVLTKKEYKVYAKKIVNATGPWVDQLR
HHHHHHCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EKDHSKNGKHLQHTKGIHLVFDQSVFPLKQAVYFDTPDGRMVFAIPREGKTYVGTTDTVY
HHHCCCCCCEEHHCCCEEEEECCCCCCHHHEEEEECCCCEEEEEECCCCCEEECCCHHHH
KEALEHPRMTTEDRDYVIKSINYMFPELNITANDIESSWAGLRPLIHEEGKDPSEISRKD
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCHHHHHCCCCHHHHCCCC
EIWTSDSGLITIAGGKLTGYRKMAEHIVDLVRDRLKEEGEKDFGPCKTKNMPISGGHVGG
CCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SKNLMSFVTAKTKEGIAAGLSEKDAKQLAIRYGSNVDRVFDRVEALKDEAAKRNIPVHIL
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
AEAEYSIEEEMTATPADFFVRRTGRLFFDINWVRTYKDAVIDFMSERFQWDEQAKNKHTE
ECCCCCHHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
NLNKLLHDAVVPLEQ
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MMNHQFSSLERDRMLTDMTKKTYDLFIIGGGITGAGTALDAASRGMKVALSEMQDFAAGT
CCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHCCC
SSRSTKLVHGGLRYLKQFEVKMVAEVGKERAIVYENGPHVTTPEWMLLPFHKGGTFGSFT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCHH
TSIGLRVYDFLAGVKKSERRSMLSAKETLQKEPLVKKDGLKGGGYYVEYRTDDARLTIEV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEHHHH
MKEAVKFGAEPVNYSKVKELLYEKGKAVGVLIEDVLTKKEYKVYAKKIVNATGPWVDQLR
HHHHHHCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EKDHSKNGKHLQHTKGIHLVFDQSVFPLKQAVYFDTPDGRMVFAIPREGKTYVGTTDTVY
HHHCCCCCCEEHHCCCEEEEECCCCCCHHHEEEEECCCCEEEEEECCCCCEEECCCHHHH
KEALEHPRMTTEDRDYVIKSINYMFPELNITANDIESSWAGLRPLIHEEGKDPSEISRKD
HHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCHHHHHCCCCHHHHCCCC
EIWTSDSGLITIAGGKLTGYRKMAEHIVDLVRDRLKEEGEKDFGPCKTKNMPISGGHVGG
CCEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
SKNLMSFVTAKTKEGIAAGLSEKDAKQLAIRYGSNVDRVFDRVEALKDEAAKRNIPVHIL
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
AEAEYSIEEEMTATPADFFVRRTGRLFFDINWVRTYKDAVIDFMSERFQWDEQAKNKHTE
ECCCCCHHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
NLNKLLHDAVVPLEQ
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2127799; 9579061; 9384377; 1809833