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The map label for this gene is glpK

Identifier: 255767217

GI number: 255767217

Start: 1003344

End: 1004834

Strand: Direct

Name: glpK

Synonym: BSU09290

Alternate gene names: 255767217

Gene position: 1003344-1004834 (Clockwise)

Preceding gene: 16077993

Following gene: 255767218

Centisome position: 23.8

GC content: 46.41

Gene sequence:

>1491_bases
ATGGAAACGTACATTTTATCCTTAGATCAGGGGACGACAAGTTCAAGAGCGATTCTGTTTAATAAAGAAGGCAAAATTGT
CCACTCTGCTCAAAAGGAATTTACACAATACTTCCCGCATCCTGGCTGGGTTGAGCATAATGCCAATGAAATTTGGGGCT
CTGTCCTCGCGGTTATCGCCTCAGTCATCTCTGAATCAGGAATCAGCGCTTCTCAAATTGCCGGCATCGGCATCACGAAC
CAGCGCGAGACGACGGTTGTGTGGGATAAAGATACAGGAAGTCCTGTCTATAATGCAATCGTTTGGCAGTCCAGACAGAC
GTCCGGCATTTGTGAGGAACTTCGTGAAAAAGGATATAATGATAAATTCAGAGAAAAAACAGGGCTTTTAATCGATCCTT
ACTTCTCCGGCACGAAGGTGAAGTGGATTTTAGACAATGTGGAAGGCGCAAGAGAAAAAGCGGAAAAAGGCGAGCTGCTG
TTTGGAACGATTGATACGTGGCTCATTTGGAAAATGTCAGGCGGAAAAGCGCATGTGACCGATTACTCCAATGCCTCAAG
AACACTGATGTTTAATATTTACGATTTAAAATGGGACGATGAACTGCTCGACATTCTAGGCGTACCGAAATCCATGCTCC
CTGAAGTGAAGCCGTCCTCTCATGTGTATGCGGAGACTGTTGATTATCACTTCTTCGGAAAAAATATCCCGATTGCTGGA
GCGGCAGGCGACCAGCAGTCCGCATTGTTCGGCCAGGCATGCTTTGAAGAAGGCATGGGGAAAAACACTTACGGCACAGG
ATGTTTCATGCTGATGAATACCGGGGAAAAAGCAATTAAGTCCGAACATGGGCTTTTGACAACAATCGCTTGGGGCATTG
ACGGAAAAGTGAACTATGCGTTAGAAGGGAGCATTTTTGTCGCAGGCTCTGCCATCCAGTGGCTTAGAGACGGTTTGAGA
ATGTTCCAGGATTCATCGCTAAGCGAATCTTATGCAGAAAAAGTGGATTCAACTGACGGCGTGTATGTTGTTCCAGCATT
TGTCGGACTGGGAACGCCTTACTGGGACAGCGATGTGCGCGGTTCGGTTTTCGGCCTGACAAGAGGGACAACAAAAGAGC
ACTTTATCCGTGCGACACTGGAGTCATTGGCTTATCAGACCAAAGATGTGCTTGACGCAATGGAAGCAGATTCAAACATT
TCATTAAAGACGCTCCGTGTAGACGGAGGAGCTGTAAAAAACAATTTCCTAATGCAGTTCCAAGGAGACCTGTTGAATGT
TCCTGTGGAGCGCCCGGAAATTAATGAAACGACTGCACTTGGCGCGGCTTATTTGGCGGGTATCGCTGTGGGATTCTGGA
AGGACCGTTCTGAAATCGCGAACCAGTGGAATCTGGATAAACGGTTTGAGCCTGAATTGGAAGAAGAAAAACGAAATGAG
CTGTATAAAGGCTGGCAAAAAGCCGTGAAAGCAGCTATGGCTTTTAAATAA

Upstream 100 bases:

>100_bases
ATTGGTTGTGGTATTGTTAGGACTCTATGTTTATACGAAATCACATTCTGCTAAAACATTATCAAATTCTAAATATATTT
AATCAAAGGGGAGACATCTT

Downstream 100 bases:

>100_bases
AGTAATACTATGGTATAATGGTTACAAGTTAATAAGAACGGTCCTGAGATGAGGAGAGACCACAGCACCAAAGTGTAAGC
ATGCACTTTGGCTGTTGTGG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
METYILSLDQGTTSSRAILFNKEGKIVHSAQKEFTQYFPHPGWVEHNANEIWGSVLAVIASVISESGISASQIAGIGITN
QRETTVVWDKDTGSPVYNAIVWQSRQTSGICEELREKGYNDKFREKTGLLIDPYFSGTKVKWILDNVEGAREKAEKGELL
FGTIDTWLIWKMSGGKAHVTDYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
AAGDQQSALFGQACFEEGMGKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVNYALEGSIFVAGSAIQWLRDGLR
MFQDSSLSESYAEKVDSTDGVYVVPAFVGLGTPYWDSDVRGSVFGLTRGTTKEHFIRATLESLAYQTKDVLDAMEADSNI
SLKTLRVDGGAVKNNFLMQFQGDLLNVPVERPEINETTALGAAYLAGIAVGFWKDRSEIANQWNLDKRFEPELEEEKRNE
LYKGWQKAVKAAMAFK

Sequences:

>Translated_496_residues
METYILSLDQGTTSSRAILFNKEGKIVHSAQKEFTQYFPHPGWVEHNANEIWGSVLAVIASVISESGISASQIAGIGITN
QRETTVVWDKDTGSPVYNAIVWQSRQTSGICEELREKGYNDKFREKTGLLIDPYFSGTKVKWILDNVEGAREKAEKGELL
FGTIDTWLIWKMSGGKAHVTDYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
AAGDQQSALFGQACFEEGMGKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVNYALEGSIFVAGSAIQWLRDGLR
MFQDSSLSESYAEKVDSTDGVYVVPAFVGLGTPYWDSDVRGSVFGLTRGTTKEHFIRATLESLAYQTKDVLDAMEADSNI
SLKTLRVDGGAVKNNFLMQFQGDLLNVPVERPEINETTALGAAYLAGIAVGFWKDRSEIANQWNLDKRFEPELEEEKRNE
LYKGWQKAVKAAMAFK
>Mature_496_residues
METYILSLDQGTTSSRAILFNKEGKIVHSAQKEFTQYFPHPGWVEHNANEIWGSVLAVIASVISESGISASQIAGIGITN
QRETTVVWDKDTGSPVYNAIVWQSRQTSGICEELREKGYNDKFREKTGLLIDPYFSGTKVKWILDNVEGAREKAEKGELL
FGTIDTWLIWKMSGGKAHVTDYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
AAGDQQSALFGQACFEEGMGKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVNYALEGSIFVAGSAIQWLRDGLR
MFQDSSLSESYAEKVDSTDGVYVVPAFVGLGTPYWDSDVRGSVFGLTRGTTKEHFIRATLESLAYQTKDVLDAMEADSNI
SLKTLRVDGGAVKNNFLMQFQGDLLNVPVERPEINETTALGAAYLAGIAVGFWKDRSEIANQWNLDKRFEPELEEEKRNE
LYKGWQKAVKAAMAFK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=500, Percent_Identity=45.2, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI42794763, Length=504, Percent_Identity=44.8412698412698, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI4504007, Length=500, Percent_Identity=45.2, Blast_Score=427, Evalue=1e-120,
Organism=Homo sapiens, GI41393575, Length=497, Percent_Identity=44.466800804829, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI88196792, Length=506, Percent_Identity=33.9920948616601, Blast_Score=323, Evalue=2e-88,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=62.2718052738337, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=502, Percent_Identity=23.3067729083665, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1787789, Length=516, Percent_Identity=23.4496124031008, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789168, Length=492, Percent_Identity=23.1707317073171, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789987, Length=506, Percent_Identity=21.9367588932806, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789138, Length=495, Percent_Identity=22.020202020202, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=41.2, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=520, Percent_Identity=25.7692307692308, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI71986147, Length=299, Percent_Identity=30.4347826086957, Blast_Score=142, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=551, Percent_Identity=35.5716878402904, Blast_Score=312, Evalue=7e-86,
Organism=Drosophila melanogaster, GI17864214, Length=504, Percent_Identity=42.2619047619048, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24654573, Length=432, Percent_Identity=44.6759259259259, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24655555, Length=496, Percent_Identity=38.1048387096774, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655560, Length=496, Percent_Identity=38.1048387096774, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655570, Length=496, Percent_Identity=38.1048387096774, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655574, Length=496, Percent_Identity=38.1048387096774, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655565, Length=496, Percent_Identity=38.1048387096774, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI19922058, Length=526, Percent_Identity=30.2281368821293, Blast_Score=265, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24652871, Length=493, Percent_Identity=31.0344827586207, Blast_Score=254, Evalue=7e-68,
Organism=Drosophila melanogaster, GI24656367, Length=493, Percent_Identity=27.789046653144, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI21357867, Length=508, Percent_Identity=26.9685039370079, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24656372, Length=340, Percent_Identity=30, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI45552909, Length=306, Percent_Identity=26.1437908496732, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55081; Mature: 55081

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METYILSLDQGTTSSRAILFNKEGKIVHSAQKEFTQYFPHPGWVEHNANEIWGSVLAVIA
CCEEEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
SVISESGISASQIAGIGITNQRETTVVWDKDTGSPVYNAIVWQSRQTSGICEELREKGYN
HHHHHCCCCHHHEEECCCCCCCCEEEEEECCCCCHHHHHEEECCCCCHHHHHHHHHCCCC
DKFREKTGLLIDPYFSGTKVKWILDNVEGAREKAEKGELLFGTIDTWLIWKMSGGKAHVT
CHHHHCCCCEEECCCCCCEEEEEEECCHHHHHHHHCCCEEEEEECEEEEEEECCCEEEEE
DYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
ECCCCCEEEEEEEEECCCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEEEEEECCCCCEEC
AAGDQQSALFGQACFEEGMGKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVNYA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEEEEE
LEGSIFVAGSAIQWLRDGLRMFQDSSLSESYAEKVDSTDGVYVVPAFVGLGTPYWDSDVR
EECCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCC
GSVFGLTRGTTKEHFIRATLESLAYQTKDVLDAMEADSNISLKTLRVDGGAVKNNFLMQF
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEE
QGDLLNVPVERPEINETTALGAAYLAGIAVGFWKDRSEIANQWNLDKRFEPELEEEKRNE
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHH
LYKGWQKAVKAAMAFK
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
METYILSLDQGTTSSRAILFNKEGKIVHSAQKEFTQYFPHPGWVEHNANEIWGSVLAVIA
CCEEEEEECCCCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
SVISESGISASQIAGIGITNQRETTVVWDKDTGSPVYNAIVWQSRQTSGICEELREKGYN
HHHHHCCCCHHHEEECCCCCCCCEEEEEECCCCCHHHHHEEECCCCCHHHHHHHHHCCCC
DKFREKTGLLIDPYFSGTKVKWILDNVEGAREKAEKGELLFGTIDTWLIWKMSGGKAHVT
CHHHHCCCCEEECCCCCCEEEEEEECCHHHHHHHHCCCEEEEEECEEEEEEECCCEEEEE
DYSNASRTLMFNIYDLKWDDELLDILGVPKSMLPEVKPSSHVYAETVDYHFFGKNIPIAG
ECCCCCEEEEEEEEECCCCHHHHHHHCCCHHHCCCCCCCCCEEEEEEEEEEECCCCCEEC
AAGDQQSALFGQACFEEGMGKNTYGTGCFMLMNTGEKAIKSEHGLLTTIAWGIDGKVNYA
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHCCCCEEEEEEECCCCEEEEE
LEGSIFVAGSAIQWLRDGLRMFQDSSLSESYAEKVDSTDGVYVVPAFVGLGTPYWDSDVR
EECCEEEECHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCC
GSVFGLTRGTTKEHFIRATLESLAYQTKDVLDAMEADSNISLKTLRVDGGAVKNNFLMQF
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEE
QGDLLNVPVERPEINETTALGAAYLAGIAVGFWKDRSEIANQWNLDKRFEPELEEEKRNE
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHH
LYKGWQKAVKAAMAFK
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA