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The map label for this gene is mdr

Identifier: 255767085

GI number: 255767085

Start: 332441

End: 333979

Strand: Reverse

Name: mdr

Synonym: BSU03070

Alternate gene names: 255767085

Gene position: 333979-332441 (Counterclockwise)

Preceding gene: 255767088

Following gene: 255767079

Centisome position: 7.92

GC content: 46.98

Gene sequence:

>1539_bases
ATGGACACAACAACAGCAAAACAGGCTTCCACCAAATTTGTGGTCCTCGGTCTCCTGCTGGGCATCTTGATGTCTGCAAT
GGACAATACGATTGTTGCCACCGCGATGGGCAATATCGTAGCCGATCTTGGAAGCTTCGATAAATTTGCCTGGGTGACGG
CATCCTATATGGTGGCGGTTATGGCTGGCATGCCGATTTACGGCAAGCTTTCCGATATGTACGGCCGAAAACGTTTTTTC
CTGTTTGGACTTATTTTTTTCTTAATCGGTTCTGCTTTATGCGGGATTGCCCAGACGATGAATCAGCTGATCATCTTCCG
GGCCATTCAAGGGATCGGAGGCGGCGCGCTCCTGCCGATTGCCTTTACCATTATCTTTGATTTGTTTCCGCCGGAAAAAC
GCGGAAAAATGTCCGGCATGTTCGGTGCTGTATTTGGATTATCCAGTGTTCTTGGTCCGCTCTTAGGCGCGATCATTACA
GATTCGATCAGCTGGCACTGGGTGTTTTACATCAATGTGCCGATCGGCGCATTGTCATTGTTTTTCATTATTCGCTACTA
CAAAGAATCTCTGGAGCACAGAAAGCAGAAAATAGACTGGGGCGGCGCGATTACCTTAGTCGTATCCATTGTATGCCTGA
TGTTCGCCCTTGAGCTTGGCGGCAAAACATACGATTGGAACTCCATTCAGATCATCGGGCTGTTTATTGTATTCGCCGTT
TTCTTCATTGCCTTTTTTATTGTGGAGAGAAAAGCAGAAGAGCCGATTATTTCTTTCTGGATGTTTAAGAACCGCTTGTT
TGCCACAGCACAGATTCTCGCTTTCCTGTATGGCGGAACATTTATTATTTTAGCGGTATTTATTCCGATTTTCGTTCAGG
CGGTCTACGGCAGCTCGGCGACAAGCGCGGGCTTCATTCTGACGCCGATGATGATTGGATCAGTCATCGGAAGCATGATC
GGCGGGATCTTCCAAACGAAGGCAAGCTTCCGCAATTTGATGCTGATATCTGTTATAGCTTTCTTTATCGGCATGCTGCT
TCTTTCCAATATGACGCCTGACACAGCACGGGTATGGCTGACAGTCTTTATGATGATTTCAGGCTTTGGTGTAGGCTTTA
ACTTCTCCCTGCTGCCGGCGGCATCGATGAATGATCTTGAACCTCGCTTTCGGGGAACGGCAAACTCTACAAATTCATTT
TTGCGATCATTTGGCATGACCTTAGGTGTCACCATTTTCGGAACGGTGCAAACAAACGTATTCACAAACAAGCTGAATGA
CGCCTTCAGCGGTATGAAAGGCTCAGCGGGATCAGGCGCTGCGCAAAATATTGGCGACCCGCAGGAGATATTCCAAGCGG
GAACACGTTCCCAGATTCCTGATGCGATCCTGAATCGCATTATTGACGCGATGTCATCAAGTATCACGTATGTCTTTTTG
CTCGCTTTGATTCCAATTGTCCTCGCGGCGGTTACCATTTTATTTATGGGAAAAGCCAGAGTGAAAACAACAGCCGAAAT
GACAAAGAAAGCAAATTAA

Upstream 100 bases:

>100_bases
CCTATAAAATCGAGTTTATCTTGATAATCGAGATATTCAACAATCAAACTATTATCATGATATCAGAACTTACATAGAAA
AGTAAGAGGAGGTAGAACAA

Downstream 100 bases:

>100_bases
CATAAAAAAAGCAGTACATGCTGGGCATGTACTGCTTTTTTCTATTACACTCCTAATCTTGCAAGAATAAACGTCCATAT
ACAAATGATCAATAACAGAA

Product: multidrug-efflux transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MDTTTAKQASTKFVVLGLLLGILMSAMDNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFF
LFGLIFFLIGSALCGIAQTMNQLIIFRAIQGIGGGALLPIAFTIIFDLFPPEKRGKMSGMFGAVFGLSSVLGPLLGAIIT
DSISWHWVFYINVPIGALSLFFIIRYYKESLEHRKQKIDWGGAITLVVSIVCLMFALELGGKTYDWNSIQIIGLFIVFAV
FFIAFFIVERKAEEPIISFWMFKNRLFATAQILAFLYGGTFIILAVFIPIFVQAVYGSSATSAGFILTPMMIGSVIGSMI
GGIFQTKASFRNLMLISVIAFFIGMLLLSNMTPDTARVWLTVFMMISGFGVGFNFSLLPAASMNDLEPRFRGTANSTNSF
LRSFGMTLGVTIFGTVQTNVFTNKLNDAFSGMKGSAGSGAAQNIGDPQEIFQAGTRSQIPDAILNRIIDAMSSSITYVFL
LALIPIVLAAVTILFMGKARVKTTAEMTKKAN

Sequences:

>Translated_512_residues
MDTTTAKQASTKFVVLGLLLGILMSAMDNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFF
LFGLIFFLIGSALCGIAQTMNQLIIFRAIQGIGGGALLPIAFTIIFDLFPPEKRGKMSGMFGAVFGLSSVLGPLLGAIIT
DSISWHWVFYINVPIGALSLFFIIRYYKESLEHRKQKIDWGGAITLVVSIVCLMFALELGGKTYDWNSIQIIGLFIVFAV
FFIAFFIVERKAEEPIISFWMFKNRLFATAQILAFLYGGTFIILAVFIPIFVQAVYGSSATSAGFILTPMMIGSVIGSMI
GGIFQTKASFRNLMLISVIAFFIGMLLLSNMTPDTARVWLTVFMMISGFGVGFNFSLLPAASMNDLEPRFRGTANSTNSF
LRSFGMTLGVTIFGTVQTNVFTNKLNDAFSGMKGSAGSGAAQNIGDPQEIFQAGTRSQIPDAILNRIIDAMSSSITYVFL
LALIPIVLAAVTILFMGKARVKTTAEMTKKAN
>Mature_512_residues
MDTTTAKQASTKFVVLGLLLGILMSAMDNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFF
LFGLIFFLIGSALCGIAQTMNQLIIFRAIQGIGGGALLPIAFTIIFDLFPPEKRGKMSGMFGAVFGLSSVLGPLLGAIIT
DSISWHWVFYINVPIGALSLFFIIRYYKESLEHRKQKIDWGGAITLVVSIVCLMFALELGGKTYDWNSIQIIGLFIVFAV
FFIAFFIVERKAEEPIISFWMFKNRLFATAQILAFLYGGTFIILAVFIPIFVQAVYGSSATSAGFILTPMMIGSVIGSMI
GGIFQTKASFRNLMLISVIAFFIGMLLLSNMTPDTARVWLTVFMMISGFGVGFNFSLLPAASMNDLEPRFRGTANSTNSF
LRSFGMTLGVTIFGTVQTNVFTNKLNDAFSGMKGSAGSGAAQNIGDPQEIFQAGTRSQIPDAILNRIIDAMSSSITYVFL
LALIPIVLAAVTILFMGKARVKTTAEMTKKAN

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family

Homologues:

Organism=Escherichia coli, GI1789042, Length=415, Percent_Identity=33.2530120481928, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1788710, Length=496, Percent_Identity=27.8225806451613, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1790195, Length=425, Percent_Identity=27.5294117647059, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788392, Length=417, Percent_Identity=28.0575539568345, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87081983, Length=312, Percent_Identity=25.6410256410256, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790146, Length=154, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788509, Length=196, Percent_Identity=27.0408163265306, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=468, Percent_Identity=25.6410256410256, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323735, Length=555, Percent_Identity=26.6666666666667, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321663, Length=542, Percent_Identity=24.169741697417, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6319783, Length=386, Percent_Identity=26.1658031088083, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319770, Length=403, Percent_Identity=26.7990074441687, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324954, Length=392, Percent_Identity=24.7448979591837, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325455, Length=447, Percent_Identity=22.8187919463087, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6323520, Length=406, Percent_Identity=26.6009852216749, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323935, Length=428, Percent_Identity=24.5327102803738, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BMR3_BACSU (P96712)

Other databases:

- EMBL:   D50098
- EMBL:   AL009126
- RefSeq:   NP_388189.2
- ProteinModelPortal:   P96712
- EnsemblBacteria:   EBBACT00000003736
- GeneID:   938345
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03070
- GenoList:   BSU03070
- GeneTree:   EBGT00070000031956
- HOGENOM:   HBG679341
- ProtClustDB:   CLSK980576
- BioCyc:   BSUB:BSU03070-MONOMER
- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- TIGRFAMs:   TIGR00711

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 55690; Mature: 55690

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xca0b664)-; HASH(0xc665b68)-; HASH(0xc777828)-; HASH(0xcc1e3bc)-; HASH(0x40a7c860)-; HASH(0xc40ca2c)-; HASH(0xaf9a4a4)-; HASH(0xbd6b870)-; HASH(0xbfd9e54)-; HASH(0xbcddea0)-; HASH(0xc619f28)-; HASH(0xc743f14)-; HASH(0xcc58cdc)-; HASH(0xc308fe4)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377