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The map label for this gene is amhX

Identifier: 255767079

GI number: 255767079

Start: 324038

End: 325189

Strand: Reverse

Name: amhX

Synonym: BSU03010

Alternate gene names: 255767079

Gene position: 325189-324038 (Counterclockwise)

Preceding gene: 255767085

Following gene: 16077366

Centisome position: 7.71

GC content: 51.22

Gene sequence:

>1152_bases
ATGCAAAAGCTTTCAGAAGAAATGAAACAAACCATTATGGACATCTTCGAGCATCTGCACGCGAACCCTGAAGTCAGCTG
GAAGGAATATGAGACAACTTCATTTTTGAAACAAAAGCTTGAGGATTTAGGGTGCCGGACGCGCACGTTTTCAGACTGCA
CCGGGGTTGTCGGCGAAATCGGCTCAGGCTCACCTGTTGTAGCGGTACGCGCTGATATTGACGCGCTGTGGCAGGAGGTT
AACGGCACATTTCGCGCCAACCATTCCTGCGGACACGATTCACATATGACAATGGCATTGGGCACGTTAATGCTGCTAAA
AAAACAACCTGAGCTTCCGAAGGGCACAATCCGTTTTATCTTTCAGCCGGCAGAAGAAAAAGGCGGCGGCGCCTTGAAAA
TGATAGAAGAAGGCGTGCTTGATGATATCGACTATTTGTATGGCGTTCACGTCCGTCCGATCCAGGAAACACAGAACGGC
CGCTGCGCTCCTTCTATTCTGCACGGATCAAGCCAGCACATTGAGGGAACGATCATCGGGGAAGAGGCTCATGGCGCCCG
TCCGCACCTCGGGAAAAACAGCATTGAAATTGCGGCATTTCTTGTACACAAGCTCGGACTCATTCATATTGACCCGCAAA
TCCCGCATACCGTCAAAATGACAAAACTGCAAGCCGGCGGTGAAAGCTCTAACATCATTCCAGGAAAAGCATCGTTCAGC
CTTGATTTGCGCGCACAGACCAATGAAGCCATGGAGGCATTAATCGCCGAAACAGAAAGAGCCTGTGAAGCGGCCGCCGC
TGCATTTGGCGCAAAGATCGAGCTGCACAAAGAACACAGCCTTCCTGCGGCAACACAAAATAAAGAAGCTGAAGCGATCA
TGGCAGAAGCCATTACGGAAATCATCGGGGCTGAGCGGCTTGATGACCCGCTTGTCACCACCGGCGGCGAGGATTTCCAT
TTTTACGCCGTGAAGGTGCCGAATCTGAAAACAACGATGCTCGGACTAGGCTGCGGGCTCCAGCCGGGGCTCCATCATCC
GCACATGACCTTTGACAGAAATGCGATGTTTACCGGCATTCATATTTTGGCGAACGCCGTGCTCAAAACATTTCAGAAGG
CGGAATCACTTGCTGCCGCTAACGCTTCTTAA

Upstream 100 bases:

>100_bases
GCAATATGTGCTATATTATATACAGCCTATCTTCCTGTTTCAATATAAATAAAGTAAAGAAGGTAAAAATTCATTCTGAT
AGAAGGAGGTATTTCTTACC

Downstream 100 bases:

>100_bases
CGCAAAAAAAGCAGCCTGACACAGGCTGCTTTTTTGATTACTTCTTCAAATCTTCAATTGAGTTGACATTTTTCATATAT
TGAGGAACAACAAGCCCCAT

Product: amidohydrolase

Products: NA

Alternate protein names: Aminoacylase

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MQKLSEEMKQTIMDIFEHLHANPEVSWKEYETTSFLKQKLEDLGCRTRTFSDCTGVVGEIGSGSPVVAVRADIDALWQEV
NGTFRANHSCGHDSHMTMALGTLMLLKKQPELPKGTIRFIFQPAEEKGGGALKMIEEGVLDDIDYLYGVHVRPIQETQNG
RCAPSILHGSSQHIEGTIIGEEAHGARPHLGKNSIEIAAFLVHKLGLIHIDPQIPHTVKMTKLQAGGESSNIIPGKASFS
LDLRAQTNEAMEALIAETERACEAAAAAFGAKIELHKEHSLPAATQNKEAEAIMAEAITEIIGAERLDDPLVTTGGEDFH
FYAVKVPNLKTTMLGLGCGLQPGLHHPHMTFDRNAMFTGIHILANAVLKTFQKAESLAAANAS

Sequences:

>Translated_383_residues
MQKLSEEMKQTIMDIFEHLHANPEVSWKEYETTSFLKQKLEDLGCRTRTFSDCTGVVGEIGSGSPVVAVRADIDALWQEV
NGTFRANHSCGHDSHMTMALGTLMLLKKQPELPKGTIRFIFQPAEEKGGGALKMIEEGVLDDIDYLYGVHVRPIQETQNG
RCAPSILHGSSQHIEGTIIGEEAHGARPHLGKNSIEIAAFLVHKLGLIHIDPQIPHTVKMTKLQAGGESSNIIPGKASFS
LDLRAQTNEAMEALIAETERACEAAAAAFGAKIELHKEHSLPAATQNKEAEAIMAEAITEIIGAERLDDPLVTTGGEDFH
FYAVKVPNLKTTMLGLGCGLQPGLHHPHMTFDRNAMFTGIHILANAVLKTFQKAESLAAANAS
>Mature_383_residues
MQKLSEEMKQTIMDIFEHLHANPEVSWKEYETTSFLKQKLEDLGCRTRTFSDCTGVVGEIGSGSPVVAVRADIDALWQEV
NGTFRANHSCGHDSHMTMALGTLMLLKKQPELPKGTIRFIFQPAEEKGGGALKMIEEGVLDDIDYLYGVHVRPIQETQNG
RCAPSILHGSSQHIEGTIIGEEAHGARPHLGKNSIEIAAFLVHKLGLIHIDPQIPHTVKMTKLQAGGESSNIIPGKASFS
LDLRAQTNEAMEALIAETERACEAAAAAFGAKIELHKEHSLPAATQNKEAEAIMAEAITEIIGAERLDDPLVTTGGEDFH
FYAVKVPNLKTTMLGLGCGLQPGLHHPHMTFDRNAMFTGIHILANAVLKTFQKAESLAAANAS

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family

Homologues:

Organism=Escherichia coli, GI87081880, Length=348, Percent_Identity=27.2988505747126, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787598, Length=309, Percent_Identity=25.2427184466019, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMHX_BACSU (P54983)

Other databases:

- EMBL:   U47860
- EMBL:   D50453
- EMBL:   AL009126
- EMBL:   U17292
- PIR:   B69585
- RefSeq:   NP_388183.2
- ProteinModelPortal:   P54983
- SMR:   P54983
- EnsemblBacteria:   EBBACT00000003045
- GeneID:   938351
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU03010
- NMPDR:   fig|224308.1.peg.302
- GenoList:   BSU03010
- GeneTree:   EBGT00070000031832
- HOGENOM:   HBG708500
- ProtClustDB:   CLSK886656
- BioCyc:   BSUB:BSU03010-MONOMER
- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650
- PIRSF:   PIRSF005962
- TIGRFAMs:   TIGR01891

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: NA

Molecular weight: Translated: 41592; Mature: 41592

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLSEEMKQTIMDIFEHLHANPEVSWKEYETTSFLKQKLEDLGCRTRTFSDCTGVVGEI
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
GSGSPVVAVRADIDALWQEVNGTFRANHSCGHDSHMTMALGTLMLLKKQPELPKGTIRFI
CCCCCEEEEECCHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
FQPAEEKGGGALKMIEEGVLDDIDYLYGVHVRPIQETQNGRCAPSILHGSSQHIEGTIIG
EECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCHHHHCCCCCCEEEEEEC
EEAHGARPHLGKNSIEIAAFLVHKLGLIHIDPQIPHTVKMTKLQAGGESSNIIPGKASFS
CCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEE
LDLRAQTNEAMEALIAETERACEAAAAAFGAKIELHKEHSLPAATQNKEAEAIMAEAITE
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
IIGAERLDDPLVTTGGEDFHFYAVKVPNLKTTMLGLGCGLQPGLHHPHMTFDRNAMFTGI
HHHHHHCCCCCEECCCCCEEEEEEECCCCCEEHEECCCCCCCCCCCCCCEECCCHHHHHH
HILANAVLKTFQKAESLAAANAS
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKLSEEMKQTIMDIFEHLHANPEVSWKEYETTSFLKQKLEDLGCRTRTFSDCTGVVGEI
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
GSGSPVVAVRADIDALWQEVNGTFRANHSCGHDSHMTMALGTLMLLKKQPELPKGTIRFI
CCCCCEEEEECCHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEE
FQPAEEKGGGALKMIEEGVLDDIDYLYGVHVRPIQETQNGRCAPSILHGSSQHIEGTIIG
EECCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCCCHHHHCCCCCCEEEEEEC
EEAHGARPHLGKNSIEIAAFLVHKLGLIHIDPQIPHTVKMTKLQAGGESSNIIPGKASFS
CCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEE
LDLRAQTNEAMEALIAETERACEAAAAAFGAKIELHKEHSLPAATQNKEAEAIMAEAITE
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHH
IIGAERLDDPLVTTGGEDFHFYAVKVPNLKTTMLGLGCGLQPGLHHPHMTFDRNAMFTGI
HHHHHHCCCCCEECCCCCEEEEEEECCCCCEEHEECCCCCCCCCCCCCCEECCCHHHHHH
HILANAVLKTFQKAESLAAANAS
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8768514; 8969502; 9384377; 7622480