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The map label for this gene is iolE

Identifier: 16081023

GI number: 16081023

Start: 4078173

End: 4079066

Strand: Reverse

Name: iolE

Synonym: BSU39720

Alternate gene names: 16081023

Gene position: 4079066-4078173 (Counterclockwise)

Preceding gene: 255767852

Following gene: 255767851

Centisome position: 96.76

GC content: 48.32

Gene sequence:

>894_bases
ATGGGCAAAAATGAAATCCTGTGGGGAATCGCTCCCATTGGGTGGCGGAATGATGACATGCCTGAAATTGGAGCGGGAAA
TACACTTCAGCATTTGTTAAGTGATATCGTTGTCGCACGTTTTCAAGGCACGGAGGTCGGGGGCTTTTTCCCCGAACCTG
CCATCCTGAACAAAGAGCTGAAGCTTCGGAACTTACGCATTGCAGGAAAATGGTTCAGCAGTTTTATTTTGCGTGACGGA
CTTGGTGAAGCGGCAAAGACATTTACCCTGCATTGTGAGTATTTGCAGCAAGTAAACGCGGATGTCGCAGTTGTCTCTGA
ACAAACGTACAGCGTGCAAAGCTTGGAGAAAAATGTGTTCACAGAGAAGCCGCACTTTACGGATGATGAATGGGAGCGGC
TTTGCGAAGGGCTGAATCACCTTGGCGAAATTGCCGCTCAGCATGGCTTGAAGCTTGTCTATCATCATCATCTCGGCACT
GGTGTCCAAACAGCGGAAGAAGTGGACCGCCTGATGGCAGGAACAGACCCTGCGCATGTACACCTCCTCTATGATACAGG
CCATGCGTATATTTCTGACGGCGATTACATGGGGATGCTTGAGAAGCATATCGGCCGCATTAAGCATGTGCACTTTAAGG
ATGCCCGCCTGAATGTCATGGAACAATGCAGGCTCGAAGGACAATCGTTCCGGCAATCATTTTTAAAAGGCATGTTTACG
GTTCCCGGTGACGGCTGCATTGACTTTAGAGAAGTATATCAGCTGCTGTTGAAGCACAGTTATTCCGGATGGATTGTCAT
TGAAGCTGAACAAGACCCCGATGTTGCAAACCCGCTGGAGTATGCATTGATTGCGAGAAACTATATTGATCAGCAGTTGT
TGGATCTGGCTTAA

Upstream 100 bases:

>100_bases
GTGGCAGAGGTATCTGAACAAGAAAGCGTTCAGAAAGCATACGAAGCAAAAGAGAAAAAGCTGGAATCTGCGAAGCAGTA
TTAGAGGGGAGGCACCATAG

Downstream 100 bases:

>100_bases
TGGGGAATGAACATATGGGCAATACCAATGGAGATTCAGCCTTCAATAAACGGACAATCGCGGCAGCATTGGCCAACTAT
ATTGATGCCGGTTCAATTGT

Product: 2-keto-myo-inositol dehydratase

Products: NA

Alternate protein names: 2-keto-myo-inositol dehydratase; 2KMI dehydratase

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MGKNEILWGIAPIGWRNDDMPEIGAGNTLQHLLSDIVVARFQGTEVGGFFPEPAILNKELKLRNLRIAGKWFSSFILRDG
LGEAAKTFTLHCEYLQQVNADVAVVSEQTYSVQSLEKNVFTEKPHFTDDEWERLCEGLNHLGEIAAQHGLKLVYHHHLGT
GVQTAEEVDRLMAGTDPAHVHLLYDTGHAYISDGDYMGMLEKHIGRIKHVHFKDARLNVMEQCRLEGQSFRQSFLKGMFT
VPGDGCIDFREVYQLLLKHSYSGWIVIEAEQDPDVANPLEYALIARNYIDQQLLDLA

Sequences:

>Translated_297_residues
MGKNEILWGIAPIGWRNDDMPEIGAGNTLQHLLSDIVVARFQGTEVGGFFPEPAILNKELKLRNLRIAGKWFSSFILRDG
LGEAAKTFTLHCEYLQQVNADVAVVSEQTYSVQSLEKNVFTEKPHFTDDEWERLCEGLNHLGEIAAQHGLKLVYHHHLGT
GVQTAEEVDRLMAGTDPAHVHLLYDTGHAYISDGDYMGMLEKHIGRIKHVHFKDARLNVMEQCRLEGQSFRQSFLKGMFT
VPGDGCIDFREVYQLLLKHSYSGWIVIEAEQDPDVANPLEYALIARNYIDQQLLDLA
>Mature_296_residues
GKNEILWGIAPIGWRNDDMPEIGAGNTLQHLLSDIVVARFQGTEVGGFFPEPAILNKELKLRNLRIAGKWFSSFILRDGL
GEAAKTFTLHCEYLQQVNADVAVVSEQTYSVQSLEKNVFTEKPHFTDDEWERLCEGLNHLGEIAAQHGLKLVYHHHLGTG
VQTAEEVDRLMAGTDPAHVHLLYDTGHAYISDGDYMGMLEKHIGRIKHVHFKDARLNVMEQCRLEGQSFRQSFLKGMFTV
PGDGCIDFREVYQLLLKHSYSGWIVIEAEQDPDVANPLEYALIARNYIDQQLLDLA

Specific function: Catalyzes the dehydration of inosose (2-keto-myo- inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi- inositol)

COG id: COG1082

COG function: function code G; Sugar phosphate isomerases/epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iolE/mocC family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IOLE_BACSU (P42416)

Other databases:

- EMBL:   D14399
- EMBL:   AL009126
- PIR:   E69645
- RefSeq:   NP_391851.1
- ProteinModelPortal:   P42416
- SMR:   P42416
- EnsemblBacteria:   EBBACT00000001938
- GeneID:   936212
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39720
- NMPDR:   fig|224308.1.peg.3977
- GenoList:   BSU39720
- GeneTree:   EBGT00050000002447
- HOGENOM:   HBG654552
- OMA:   DLGKENT
- ProtClustDB:   CLSK873288
- BioCyc:   BSUB:BSU39720-MONOMER
- HAMAP:   MF_01672
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =4.2.1.44

Molecular weight: Translated: 33589; Mature: 33457

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNEILWGIAPIGWRNDDMPEIGAGNTLQHLLSDIVVARFQGTEVGGFFPEPAILNKEL
CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCH
KLRNLRIAGKWFSSFILRDGLGEAAKTFTLHCEYLQQVNADVAVVSEQTYSVQSLEKNVF
HHHEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHC
TEKPHFTDDEWERLCEGLNHLGEIAAQHGLKLVYHHHLGTGVQTAEEVDRLMAGTDPAHV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEE
HLLYDTGHAYISDGDYMGMLEKHIGRIKHVHFKDARLNVMEQCRLEGQSFRQSFLKGMFT
EEEEECCCEEECCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCHHHHHHHHHHCEEC
VPGDGCIDFREVYQLLLKHSYSGWIVIEAEQDPDVANPLEYALIARNYIDQQLLDLA
CCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKNEILWGIAPIGWRNDDMPEIGAGNTLQHLLSDIVVARFQGTEVGGFFPEPAILNKEL
CCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCH
KLRNLRIAGKWFSSFILRDGLGEAAKTFTLHCEYLQQVNADVAVVSEQTYSVQSLEKNVF
HHHEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHC
TEKPHFTDDEWERLCEGLNHLGEIAAQHGLKLVYHHHLGTGVQTAEEVDRLMAGTDPAHV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEE
HLLYDTGHAYISDGDYMGMLEKHIGRIKHVHFKDARLNVMEQCRLEGQSFRQSFLKGMFT
EEEEECCCEEECCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCHHHHHHHHHHCEEC
VPGDGCIDFREVYQLLLKHSYSGWIVIEAEQDPDVANPLEYALIARNYIDQQLLDLA
CCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7952181; 9384377