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The map label for this gene is iolD

Identifier: 255767852

GI number: 255767852

Start: 4079083

End: 4080996

Strand: Reverse

Name: iolD

Synonym: BSU39730

Alternate gene names: 255767852

Gene position: 4080996-4079083 (Counterclockwise)

Preceding gene: 16081025

Following gene: 16081023

Centisome position: 96.81

GC content: 48.64

Gene sequence:

>1914_bases
GTGGGTAAGAAAATTCGCTTAACAACTGCGCAGGCGCTGATTAAATTCTTAAACCAGCAGTACATCCATGTAGATGGAAA
GGAAGAGCCTTTTGTCGAAGGGATCTTCACGATTTTCGGTCATGGAAACGTATTAGGGATCGGGCAGGCGCTTGAGCAGG
ATGCAGGCCATTTGAAAGTCTATCAAGGGAAAAATGAACAAGGAATGGCGCATGCGGCCATGGCGTACAGCAAGCAAATG
CTGAGAAGAAAAATATATGCGGTGTCTACATCCGTCGGACCTGGAGCGGCTAACTTGGTGGCGGCAGCCGGCACTGCATT
GGCAAATAATATCCCAGTTCTCTTGATTCCGGCAGACACATTTGCGACAAGACAGCCAGACCCTGTACTGCAGCAAATGG
AGCAAGAATACAGTGCGGCGATTACGACAAACGATGCATTGAAGCCTGTATCGAGATACTGGGACCGCATTACGCGCCCT
GAGCAGCTGATGAGCAGTTTGCTGCGCGCGTTTGAAGTCATGACTGATCCGGCAAAAGCAGGTCCGGCAACGATTTGTAT
TTCTCAGGATGTTGAAGGGGAAGCATACGATTTTGATGAAAGTTTCTTCGTCAAACGGGTTCACTATATTGATCGCATGC
AGCCGAGCGAGCGCGAGCTTCAAGGTGCGGCGGAGCTGATTAAATCCAGCAAAAAACCTGTGATTCTCGTCGGCGGAGGC
GCAAAATATTCCGGTGCGCGCGATGAATTGGTTGCCATTTCTGAAGCCTATAACATTCCGTTAGTTGAAACGCAGGCAGG
AAAGTCTACCGTTGAGGCAGATTTTGCGAACAACCTTGGCGGAATGGGCATCACAGGTACACTTGCGGCAAACAAAGCGG
CCCGCCAAGCTGATTTGATTATCGGCATCGGCACAAGGTATACAGATTTTGCGACATCCTCTAAGACCGCTTTTGATTTT
GATAAAGCGAAGTTTTTGAACATTAACGTCAGCCGAATGCAGGCGTATAAACTTGATGCATTCCAAGTGGTGGCGGATGC
GAAAGTGACGCTTGGCAAACTGCACGGCTTGCTTGAAGGCTATGAGAGCGAGTTCGGCACAACGATCCGGGAGCTGAAGG
ACGAATGGCTAGCTGAACGCGAGCGTCTCAGCAAAGTGACGTTTAAGCGGGAAGCATTTGATCCGGAAATCAAAAATCAC
TTTTCTCAAGAGGTCCTGAATGAATATGCTGACGCGTTGAATACGGAGCTTCCGCAAACAACGGCATTGCTGACAATCAA
CGAGACGATTCCTGAAGACAGCGTCATCATCTGTTCAGCAGGCTCACTCCCAGGAGATTTGCAGCGTCTGTGGCATTCTA
ATGTTCCGAATACGTATCACCTGGAGTATGGATATTCTTGCATGGGCTATGAAGTGTCCGGGACACTCGGTCTTAAACTG
GCCCACCCTGACAGAGAAGTGTATTCAATCGTCGGAGACGGCAGCTTCCTGATGCTTCATTCTGAACTGATCACGGCGAT
TCAGTACAACAAGAAAATCAATGTGCTGCTCTTTGACAACTCAGGATTCGGATGCATCAACAACCTGCAAATGGATCACG
GCAGCGGCAGCTACTATTGCGAGTTCCGCACAGATGACAACCAAATCCTGAATGTCGATTACGCGAAAGTCGCTGAGGGA
TACGGCGCGAAAACCTACCGTGCAAACACAGTAGAAGAATTAAAAGCTGCGTTAGAGGATGCGAAGAAACAGGATGTATC
AACATTAATTGAAATGAAGGTGCTGCCTAAAACAATGACGGACGGCTATGACAGCTGGTGGCATGTCGGGGTGGCAGAGG
TATCTGAACAAGAAAGCGTTCAGAAAGCATACGAAGCAAAAGAGAAAAAGCTGGAATCTGCGAAGCAGTATTAG

Upstream 100 bases:

>100_bases
AGCACAGTTCGTCAGAAGCGATGCCGACTGCGGAAGAAATCGAACAGCTTATTGAAGCACAGTCATAATTGAAAAAGATC
GAGTAAAGGTGGGATCGTTT

Downstream 100 bases:

>100_bases
AGGGGAGGCACCATAGATGGGCAAAAATGAAATCCTGTGGGGAATCGCTCCCATTGGGTGGCGGAATGATGACATGCCTG
AAATTGGAGCGGGAAATACA

Product: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase

Products: NA

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MGKKIRLTTAQALIKFLNQQYIHVDGKEEPFVEGIFTIFGHGNVLGIGQALEQDAGHLKVYQGKNEQGMAHAAMAYSKQM
LRRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADTFATRQPDPVLQQMEQEYSAAITTNDALKPVSRYWDRITRP
EQLMSSLLRAFEVMTDPAKAGPATICISQDVEGEAYDFDESFFVKRVHYIDRMQPSERELQGAAELIKSSKKPVILVGGG
AKYSGARDELVAISEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARQADLIIGIGTRYTDFATSSKTAFDF
DKAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEGYESEFGTTIRELKDEWLAERERLSKVTFKREAFDPEIKNH
FSQEVLNEYADALNTELPQTTALLTINETIPEDSVIICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKL
AHPDREVYSIVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGSGSYYCEFRTDDNQILNVDYAKVAEG
YGAKTYRANTVEELKAALEDAKKQDVSTLIEMKVLPKTMTDGYDSWWHVGVAEVSEQESVQKAYEAKEKKLESAKQY

Sequences:

>Translated_637_residues
MGKKIRLTTAQALIKFLNQQYIHVDGKEEPFVEGIFTIFGHGNVLGIGQALEQDAGHLKVYQGKNEQGMAHAAMAYSKQM
LRRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADTFATRQPDPVLQQMEQEYSAAITTNDALKPVSRYWDRITRP
EQLMSSLLRAFEVMTDPAKAGPATICISQDVEGEAYDFDESFFVKRVHYIDRMQPSERELQGAAELIKSSKKPVILVGGG
AKYSGARDELVAISEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARQADLIIGIGTRYTDFATSSKTAFDF
DKAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEGYESEFGTTIRELKDEWLAERERLSKVTFKREAFDPEIKNH
FSQEVLNEYADALNTELPQTTALLTINETIPEDSVIICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKL
AHPDREVYSIVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGSGSYYCEFRTDDNQILNVDYAKVAEG
YGAKTYRANTVEELKAALEDAKKQDVSTLIEMKVLPKTMTDGYDSWWHVGVAEVSEQESVQKAYEAKEKKLESAKQY
>Mature_636_residues
GKKIRLTTAQALIKFLNQQYIHVDGKEEPFVEGIFTIFGHGNVLGIGQALEQDAGHLKVYQGKNEQGMAHAAMAYSKQML
RRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADTFATRQPDPVLQQMEQEYSAAITTNDALKPVSRYWDRITRPE
QLMSSLLRAFEVMTDPAKAGPATICISQDVEGEAYDFDESFFVKRVHYIDRMQPSERELQGAAELIKSSKKPVILVGGGA
KYSGARDELVAISEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARQADLIIGIGTRYTDFATSSKTAFDFD
KAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEGYESEFGTTIRELKDEWLAERERLSKVTFKREAFDPEIKNHF
SQEVLNEYADALNTELPQTTALLTINETIPEDSVIICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKLA
HPDREVYSIVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGSGSYYCEFRTDDNQILNVDYAKVAEGY
GAKTYRANTVEELKAALEDAKKQDVSTLIEMKVLPKTMTDGYDSWWHVGVAEVSEQESVQKAYEAKEKKLESAKQY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=595, Percent_Identity=22.0168067226891, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI21361361, Length=604, Percent_Identity=22.5165562913907, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1790104, Length=598, Percent_Identity=22.4080267558528, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=24.7330960854093, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1786717, Length=563, Percent_Identity=22.5577264653641, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1787096, Length=521, Percent_Identity=22.2648752399232, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788716, Length=521, Percent_Identity=20.1535508637236, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=590, Percent_Identity=23.728813559322, Blast_Score=130, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.-.-.-

Molecular weight: Translated: 70380; Mature: 70249

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKIRLTTAQALIKFLNQQYIHVDGKEEPFVEGIFTIFGHGNVLGIGQALEQDAGHLKV
CCCEEEEHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEEECCHHHHCCCCCEEE
YQGKNEQGMAHAAMAYSKQMLRRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADT
EECCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCC
FATRQPDPVLQQMEQEYSAAITTNDALKPVSRYWDRITRPEQLMSSLLRAFEVMTDPAKA
CCCCCCCHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
GPATICISQDVEGEAYDFDESFFVKRVHYIDRMQPSERELQGAAELIKSSKKPVILVGGG
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECC
AKYSGARDELVAISEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARQADLI
CCCCCCCCCEEEEHHHCCCCEEEECCCCCCCHHHHHHCCCCCCEEEEHHHCHHHCCCCEE
IGIGTRYTDFATSSKTAFDFDKAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEG
EECCCCCCCCCCCCCCEECCCCEEEEEEEHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHH
YESEFGTTIRELKDEWLAERERLSKVTFKREAFDPEIKNHFSQEVLNEYADALNTELPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
TALLTINETIPEDSVIICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKL
EEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEECCEEEEEE
AHPDREVYSIVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGSGSYYC
ECCCHHHEEEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCEECCCEEECCCCCEEE
EFRTDDNQILNVDYAKVAEGYGAKTYRANTVEELKAALEDAKKQDVSTLIEMKVLPKTMT
EEECCCCCEEECCHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
DGYDSWWHVGVAEVSEQESVQKAYEAKEKKLESAKQY
CCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKKIRLTTAQALIKFLNQQYIHVDGKEEPFVEGIFTIFGHGNVLGIGQALEQDAGHLKV
CCEEEEHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCCCCCEEECCHHHHCCCCCEEE
YQGKNEQGMAHAAMAYSKQMLRRKIYAVSTSVGPGAANLVAAAGTALANNIPVLLIPADT
EECCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCC
FATRQPDPVLQQMEQEYSAAITTNDALKPVSRYWDRITRPEQLMSSLLRAFEVMTDPAKA
CCCCCCCHHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
GPATICISQDVEGEAYDFDESFFVKRVHYIDRMQPSERELQGAAELIKSSKKPVILVGGG
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECC
AKYSGARDELVAISEAYNIPLVETQAGKSTVEADFANNLGGMGITGTLAANKAARQADLI
CCCCCCCCCEEEEHHHCCCCEEEECCCCCCCHHHHHHCCCCCCEEEEHHHCHHHCCCCEE
IGIGTRYTDFATSSKTAFDFDKAKFLNINVSRMQAYKLDAFQVVADAKVTLGKLHGLLEG
EECCCCCCCCCCCCCCEECCCCEEEEEEEHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHH
YESEFGTTIRELKDEWLAERERLSKVTFKREAFDPEIKNHFSQEVLNEYADALNTELPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
TALLTINETIPEDSVIICSAGSLPGDLQRLWHSNVPNTYHLEYGYSCMGYEVSGTLGLKL
EEEEEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCCEEEEEEECCEEEEEE
AHPDREVYSIVGDGSFLMLHSELITAIQYNKKINVLLFDNSGFGCINNLQMDHGSGSYYC
ECCCHHHEEEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCEECCCEEECCCCCEEE
EFRTDDNQILNVDYAKVAEGYGAKTYRANTVEELKAALEDAKKQDVSTLIEMKVLPKTMT
EEECCCCCEEECCHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
DGYDSWWHVGVAEVSEQESVQKAYEAKEKKLESAKQY
CCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA